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The map label for this gene is ycdT [H]

Identifier: 238895023

GI number: 238895023

Start: 2909223

End: 2910692

Strand: Direct

Name: ycdT [H]

Synonym: KP1_3053

Alternate gene names: 238895023

Gene position: 2909223-2910692 (Clockwise)

Preceding gene: 238895022

Following gene: 238895025

Centisome position: 55.43

GC content: 49.52

Gene sequence:

>1470_bases
TTGCGCCTGCGCAATATCTTATCTGCCCGCCCCTATTACCCTCAAGAACGTAAATTAGCCCTGGTGACTCATCGTCGCTG
CCGTTGCCTTTTATGTGGAATATTGCCTATGCCCGAACATGTGCATCATATCGTTAAACGTCTAATCCTTTATATTTGCA
TTTGTTTACTGGCCGGGATTGGCATTTCCGGGATGACTGCTTTTTTTCATACCAGCTTCAGTAGTACTTCCTCAATATTA
TTTCCGCTGCTGACCAGTTTCCTGATGGTTTTTCATATTACCATTGCCTGTTTTATGGGCATGAAATACTGGAGCAGCAA
GCGGCGTCTGTACCTTACTCCAGTCTCTTTCGGGTTTGCCTGCTCAGCGCTGCTGATGCTTGGCACGCTCAGCAGCTATC
CGGACTGGCTTACCTGCAATCCGGCCCCGGTCGTTAATCAAAATGACGCCGTTATCTATTATTTCTTCCGCAATATAATG
ATGGCTGTGTTATTCATGTCATCTATTATTTTGTACTATTTTCGCCAGCGCATCATGCATTCGTGGAAAGCACACGTTCT
TACATTTACAGCATGTATACTTTTTACGTTGACCATTATTGTTTTATCATGGCTATACTCCAGCCATTCGCCGTGGCTGA
GCGTCAATTTTATCGACGATCTGAGCCATACCTTTACCCCGTTGTGGCAAAGTATTATCGGCTGGCTGCTGATGGCAGTT
TGGTTCATCACGCTGATTTTATTAATTAGCTTGAGTAAACTGCGTAATATTTTTTGGTTCAGCGGCGCTTTTTTTTGCAG
CGCTTATTTGTTCACGCTTTTCCAGCTGCTCTCCACCGCAGGGGAGCTGGATCAGACCTGGTACCAGGCGCGTTTTTTCG
AAACGCTATGCACCTTGTTCCTGATTCTGGTGCTACTGGTTGACGTCTTTATCCTCTATCGCGAGTCTAACCACAAATAT
GTCCATTCGTATCAGAATTCGATCCGCGACCCGTTGACCCGCCTCTACAACCGCAGCTTTTTCTACGACACTCTTAACCA
ACAGCTGGCGAAGGTGAATGCCCAGCATCCGCTGTCGGTGCTCATCAGCGACCTCGACCATTTCAAACGCATCAACGATA
GCTACGGCCATGTCGCCGGCGACAAAGTGATCCAGTTCGCCGCCTCGGTGCTGGAAAGCCATTCGCGGGTAGATGACGCC
GCCGCACGCATCGGCGGTGAGGAATTTGCTCTGCTGCTGGTCAACACCGGTGAAAAAGAAGCGCAAGCGATCGCCGAGCG
TATCCGCCTGGCGGTCAGTGCCGGGGAGAGCCATTTACCGGAGCGAATGACCATCAGCATGGGGGTGTATACCACCTACG
ACAATAGCGTGACAGCGGAAGCGTGCGTTCAGCGCGCGGACGAAGCCATGTATGAAGCAAAGAATAACGGACGTAACCAA
GTGATCGTCTGGCATCAGCAGGGAGGGTGA

Upstream 100 bases:

>100_bases
CGGCCGGTTGCGCCGCATCGTATTTTTCAGCCACGTTTATTTCGCCGTAATAAATTCGTAGTAACCATCAGACATCTTTA
TTTTAATATTCCATGAATCA

Downstream 100 bases:

>100_bases
AAAAAGGCCCGGATTAGCCCGGGCCGCAAAGAATTAATCTTTAGCCTGGAGATCGAGGATATTTTGTTCAAGTTTACGGA
TTAAGTGGAGCAAGGTTTCC

Product: hypothetical protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MRLRNILSARPYYPQERKLALVTHRRCRCLLCGILPMPEHVHHIVKRLILYICICLLAGIGISGMTAFFHTSFSSTSSIL
FPLLTSFLMVFHITIACFMGMKYWSSKRRLYLTPVSFGFACSALLMLGTLSSYPDWLTCNPAPVVNQNDAVIYYFFRNIM
MAVLFMSSIILYYFRQRIMHSWKAHVLTFTACILFTLTIIVLSWLYSSHSPWLSVNFIDDLSHTFTPLWQSIIGWLLMAV
WFITLILLISLSKLRNIFWFSGAFFCSAYLFTLFQLLSTAGELDQTWYQARFFETLCTLFLILVLLVDVFILYRESNHKY
VHSYQNSIRDPLTRLYNRSFFYDTLNQQLAKVNAQHPLSVLISDLDHFKRINDSYGHVAGDKVIQFAASVLESHSRVDDA
AARIGGEEFALLLVNTGEKEAQAIAERIRLAVSAGESHLPERMTISMGVYTTYDNSVTAEACVQRADEAMYEAKNNGRNQ
VIVWHQQGG

Sequences:

>Translated_489_residues
MRLRNILSARPYYPQERKLALVTHRRCRCLLCGILPMPEHVHHIVKRLILYICICLLAGIGISGMTAFFHTSFSSTSSIL
FPLLTSFLMVFHITIACFMGMKYWSSKRRLYLTPVSFGFACSALLMLGTLSSYPDWLTCNPAPVVNQNDAVIYYFFRNIM
MAVLFMSSIILYYFRQRIMHSWKAHVLTFTACILFTLTIIVLSWLYSSHSPWLSVNFIDDLSHTFTPLWQSIIGWLLMAV
WFITLILLISLSKLRNIFWFSGAFFCSAYLFTLFQLLSTAGELDQTWYQARFFETLCTLFLILVLLVDVFILYRESNHKY
VHSYQNSIRDPLTRLYNRSFFYDTLNQQLAKVNAQHPLSVLISDLDHFKRINDSYGHVAGDKVIQFAASVLESHSRVDDA
AARIGGEEFALLLVNTGEKEAQAIAERIRLAVSAGESHLPERMTISMGVYTTYDNSVTAEACVQRADEAMYEAKNNGRNQ
VIVWHQQGG
>Mature_489_residues
MRLRNILSARPYYPQERKLALVTHRRCRCLLCGILPMPEHVHHIVKRLILYICICLLAGIGISGMTAFFHTSFSSTSSIL
FPLLTSFLMVFHITIACFMGMKYWSSKRRLYLTPVSFGFACSALLMLGTLSSYPDWLTCNPAPVVNQNDAVIYYFFRNIM
MAVLFMSSIILYYFRQRIMHSWKAHVLTFTACILFTLTIIVLSWLYSSHSPWLSVNFIDDLSHTFTPLWQSIIGWLLMAV
WFITLILLISLSKLRNIFWFSGAFFCSAYLFTLFQLLSTAGELDQTWYQARFFETLCTLFLILVLLVDVFILYRESNHKY
VHSYQNSIRDPLTRLYNRSFFYDTLNQQLAKVNAQHPLSVLISDLDHFKRINDSYGHVAGDKVIQFAASVLESHSRVDDA
AARIGGEEFALLLVNTGEKEAQAIAERIRLAVSAGESHLPERMTISMGVYTTYDNSVTAEACVQRADEAMYEAKNNGRNQ
VIVWHQQGG

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=346, Percent_Identity=29.7687861271676, Blast_Score=165, Evalue=8e-42,
Organism=Escherichia coli, GI1788085, Length=404, Percent_Identity=27.2277227722772, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI87082007, Length=199, Percent_Identity=38.1909547738693, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI1788381, Length=165, Percent_Identity=40, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI87081881, Length=156, Percent_Identity=41.6666666666667, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI145693134, Length=163, Percent_Identity=36.8098159509202, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1787802, Length=158, Percent_Identity=33.5443037974684, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1787541, Length=164, Percent_Identity=32.3170731707317, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1787816, Length=165, Percent_Identity=33.3333333333333, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1786584, Length=162, Percent_Identity=32.0987654320988, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI87081974, Length=160, Percent_Identity=33.125, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788956, Length=169, Percent_Identity=31.3609467455621, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1787056, Length=178, Percent_Identity=29.2134831460674, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87081977, Length=178, Percent_Identity=28.6516853932584, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 56120; Mature: 56120

Theoretical pI: Translated: 8.51; Mature: 8.51

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLRNILSARPYYPQERKLALVTHRRCRCLLCGILPMPEHVHHIVKRLILYICICLLAGI
CCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCC
GISGMTAFFHTSFSSTSSILFPLLTSFLMVFHITIACFMGMKYWSSKRRLYLTPVSFGFA
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHH
CSALLMLGTLSSYPDWLTCNPAPVVNQNDAVIYYFFRNIMMAVLFMSSIILYYFRQRIMH
HHHHHHHHHHHCCCCCEECCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SWKAHVLTFTACILFTLTIIVLSWLYSSHSPWLSVNFIDDLSHTFTPLWQSIIGWLLMAV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHH
WFITLILLISLSKLRNIFWFSGAFFCSAYLFTLFQLLSTAGELDQTWYQARFFETLCTLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LILVLLVDVFILYRESNHKYVHSYQNSIRDPLTRLYNRSFFYDTLNQQLAKVNAQHPLSV
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHH
LISDLDHFKRINDSYGHVAGDKVIQFAASVLESHSRVDDAAARIGGEEFALLLVNTGEKE
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHH
AQAIAERIRLAVSAGESHLPERMTISMGVYTTYDNSVTAEACVQRADEAMYEAKNNGRNQ
HHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCE
VIVWHQQGG
EEEEECCCC
>Mature Secondary Structure
MRLRNILSARPYYPQERKLALVTHRRCRCLLCGILPMPEHVHHIVKRLILYICICLLAGI
CCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCC
GISGMTAFFHTSFSSTSSILFPLLTSFLMVFHITIACFMGMKYWSSKRRLYLTPVSFGFA
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHH
CSALLMLGTLSSYPDWLTCNPAPVVNQNDAVIYYFFRNIMMAVLFMSSIILYYFRQRIMH
HHHHHHHHHHHCCCCCEECCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SWKAHVLTFTACILFTLTIIVLSWLYSSHSPWLSVNFIDDLSHTFTPLWQSIIGWLLMAV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHH
WFITLILLISLSKLRNIFWFSGAFFCSAYLFTLFQLLSTAGELDQTWYQARFFETLCTLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LILVLLVDVFILYRESNHKYVHSYQNSIRDPLTRLYNRSFFYDTLNQQLAKVNAQHPLSV
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHH
LISDLDHFKRINDSYGHVAGDKVIQFAASVLESHSRVDDAAARIGGEEFALLLVNTGEKE
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHH
AQAIAERIRLAVSAGESHLPERMTISMGVYTTYDNSVTAEACVQRADEAMYEAKNNGRNQ
HHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCE
VIVWHQQGG
EEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]