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The map label for this gene is rihA [H]

Identifier: 238894950

GI number: 238894950

Start: 2840409

End: 2841509

Strand: Direct

Name: rihA [H]

Synonym: KP1_2975

Alternate gene names: 238894950

Gene position: 2840409-2841509 (Clockwise)

Preceding gene: 238894948

Following gene: 238894953

Centisome position: 54.12

GC content: 61.22

Gene sequence:

>1101_bases
TTGCGCCACCGCAAAACGAGGCACCATGTTTACGGACGGTCGCGTAACGATATGCGTTGCCCTCGCAGCAATCGTGGTCT
CCATCACACTATTCTGCACACCATCTCGTCTGCCTCGCCTCACAGCGTTAATCTGGTCACAGAACAACGCCAACAAGGAG
CTGCTATGGCACTGCCGATTATGATTGATTGCGATCCGGGACATGATGACGCGATCGCCCTGGTCCTCGCCCTCGCCTCG
CCGGAGCTGGAGGTGAAAGCCGTCACCGCTTCCGCGGGCAACCAGACCCCGGAGAAGACCCTGCGCAACGTGCTGCGTAT
GTTGACGCTGCTGAACCGGCCGGACATTCCGGTTGCCGGCGGCGCGTGGAAGCCGTTGATGCGCGATCTGATCATCGCCG
ATAACGTGCACGGCGAGAGCGGACTGGATGGCCCGAGCCTGCCTGAGCCGGCCTTTGCGCCGCAGAATTGCACCGCCGTC
GAGCTGATGGCCAGCGTGCTGCGGGAAAGCCAGGAGCCGGTGACTCTGGTGGCCACCGGACCGCAGACTAACGTGGCGTT
GCTGCTCGCCAGCCATCCGGAGCTGCACGCGAAGATCGCCCGCATCGTGATCATGGGGGGCGCCATGGGGCTGGGCAACT
GGCAGCCGGCGGCGGAGTTTAATATCTACGTTGATCCGCAGGCCGCGGAAATGGTCTTTCAGTCGGGGATCCCGGTGGTG
ATGGCGGGCCTTGACGTCACCCATAAGGCGCAAATCCTTCCCGCCGATATCGAGCGATTCCGCCAGATTGGCAACCCGGT
GTCGACCATCGTCGCCGAACTGCTCGACTTCTTTATGGCCTACCACAAAGATGAAAAGTGGGGCTTCGACGGCGCTCCGC
TGCACGATCCCTGCACCATCGCCTGGCTCCTCAAGCCGGAGATCTTTACCACGATCGAACGCTGGGTTGGCGTAGAAACG
GAAGGCAAATATACCCAGGGGATGACGGTGGTCGATTACTACCATCTGACCGGCAATCGGCCCAATACGACCCTGATGCT
GGATGTGGACCGCGAAGCCTTTGTCGATCTGCTCGCCCAGCGGTTAGCCTTCTACGCCTGA

Upstream 100 bases:

>100_bases
GTTTTCTTAAGGATAAGAACCCGCCCGCCGAAAAGCAAACCATTAATATCCGTAAGGATTAGCAATTTTTCTATTATATC
GCGCCGCGCCGGGCGCCATC

Downstream 100 bases:

>100_bases
CCTCTATGCCAGGCCCCTTTCCCCTGGCGGAAAAAGGGGCCTGTTCAGTCGAAGCCCGCCAGGTTAGTCGTCCGCCGCGA
CGCGGATCACTACCTTACCG

Product: ribonucleoside hydrolase 1

Products: uracil; ribose; cytosine [C]

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MRHRKTRHHVYGRSRNDMRCPRSNRGLHHTILHTISSASPHSVNLVTEQRQQGAAMALPIMIDCDPGHDDAIALVLALAS
PELEVKAVTASAGNQTPEKTLRNVLRMLTLLNRPDIPVAGGAWKPLMRDLIIADNVHGESGLDGPSLPEPAFAPQNCTAV
ELMASVLRESQEPVTLVATGPQTNVALLLASHPELHAKIARIVIMGGAMGLGNWQPAAEFNIYVDPQAAEMVFQSGIPVV
MAGLDVTHKAQILPADIERFRQIGNPVSTIVAELLDFFMAYHKDEKWGFDGAPLHDPCTIAWLLKPEIFTTIERWVGVET
EGKYTQGMTVVDYYHLTGNRPNTTLMLDVDREAFVDLLAQRLAFYA

Sequences:

>Translated_366_residues
MRHRKTRHHVYGRSRNDMRCPRSNRGLHHTILHTISSASPHSVNLVTEQRQQGAAMALPIMIDCDPGHDDAIALVLALAS
PELEVKAVTASAGNQTPEKTLRNVLRMLTLLNRPDIPVAGGAWKPLMRDLIIADNVHGESGLDGPSLPEPAFAPQNCTAV
ELMASVLRESQEPVTLVATGPQTNVALLLASHPELHAKIARIVIMGGAMGLGNWQPAAEFNIYVDPQAAEMVFQSGIPVV
MAGLDVTHKAQILPADIERFRQIGNPVSTIVAELLDFFMAYHKDEKWGFDGAPLHDPCTIAWLLKPEIFTTIERWVGVET
EGKYTQGMTVVDYYHLTGNRPNTTLMLDVDREAFVDLLAQRLAFYA
>Mature_366_residues
MRHRKTRHHVYGRSRNDMRCPRSNRGLHHTILHTISSASPHSVNLVTEQRQQGAAMALPIMIDCDPGHDDAIALVLALAS
PELEVKAVTASAGNQTPEKTLRNVLRMLTLLNRPDIPVAGGAWKPLMRDLIIADNVHGESGLDGPSLPEPAFAPQNCTAV
ELMASVLRESQEPVTLVATGPQTNVALLLASHPELHAKIARIVIMGGAMGLGNWQPAAEFNIYVDPQAAEMVFQSGIPVV
MAGLDVTHKAQILPADIERFRQIGNPVSTIVAELLDFFMAYHKDEKWGFDGAPLHDPCTIAWLLKPEIFTTIERWVGVET
EGKYTQGMTVVDYYHLTGNRPNTTLMLDVDREAFVDLLAQRLAFYA

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=311, Percent_Identity=86.4951768488746, Blast_Score=569, Evalue=1e-163,
Organism=Escherichia coli, GI1788486, Length=306, Percent_Identity=38.562091503268, Blast_Score=224, Evalue=7e-60,
Organism=Escherichia coli, GI1786213, Length=309, Percent_Identity=42.7184466019417, Blast_Score=213, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI32565474, Length=188, Percent_Identity=31.3829787234043, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17565698, Length=188, Percent_Identity=34.0425531914894, Blast_Score=88, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6320608, Length=259, Percent_Identity=35.5212355212355, Blast_Score=144, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24641839, Length=191, Percent_Identity=32.9842931937173, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24583915, Length=326, Percent_Identity=25.4601226993865, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24641837, Length=316, Percent_Identity=27.8481012658228, Blast_Score=81, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 40247; Mature: 40247

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS01247 IUNH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHRKTRHHVYGRSRNDMRCPRSNRGLHHTILHTISSASPHSVNLVTEQRQQGAAMALPI
CCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEHHHHHCCCEEEEEE
MIDCDPGHDDAIALVLALASPELEVKAVTASAGNQTPEKTLRNVLRMLTLLNRPDIPVAG
EEECCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
GAWKPLMRDLIIADNVHGESGLDGPSLPEPAFAPQNCTAVELMASVLRESQEPVTLVATG
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEC
PQTNVALLLASHPELHAKIARIVIMGGAMGLGNWQPAAEFNIYVDPQAAEMVFQSGIPVV
CCCCEEEEEECCCHHHHHHHHHEEECCCCCCCCCCCCCEEEEEECCHHHHHHHHCCCCEE
MAGLDVTHKAQILPADIERFRQIGNPVSTIVAELLDFFMAYHKDEKWGFDGAPLHDPCTI
EECCCCCCCCEECHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEE
AWLLKPEIFTTIERWVGVETEGKYTQGMTVVDYYHLTGNRPNTTLMLDVDREAFVDLLAQ
EEEECCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCEEEEEECCHHHHHHHHHH
RLAFYA
HHHHCC
>Mature Secondary Structure
MRHRKTRHHVYGRSRNDMRCPRSNRGLHHTILHTISSASPHSVNLVTEQRQQGAAMALPI
CCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEHHHHHCCCEEEEEE
MIDCDPGHDDAIALVLALASPELEVKAVTASAGNQTPEKTLRNVLRMLTLLNRPDIPVAG
EEECCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
GAWKPLMRDLIIADNVHGESGLDGPSLPEPAFAPQNCTAVELMASVLRESQEPVTLVATG
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEC
PQTNVALLLASHPELHAKIARIVIMGGAMGLGNWQPAAEFNIYVDPQAAEMVFQSGIPVV
CCCCEEEEEECCCHHHHHHHHHEEECCCCCCCCCCCCCEEEEEECCHHHHHHHHCCCCEE
MAGLDVTHKAQILPADIERFRQIGNPVSTIVAELLDFFMAYHKDEKWGFDGAPLHDPCTI
EECCCCCCCCEECHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEE
AWLLKPEIFTTIERWVGVETEGKYTQGMTVVDYYHLTGNRPNTTLMLDVDREAFVDLLAQ
EEEECCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCEEEEEECCHHHHHHHHHH
RLAFYA
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine; H2O; cytidine [C]

Specific reaction: uridine + H2O = uracil + ribose cytidine + H2O = cytosine + ribose [C]

General reaction: Pyrimidine-specific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA