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The map label for this gene is narY [H]

Identifier: 238894924

GI number: 238894924

Start: 2813361

End: 2814905

Strand: Direct

Name: narY [H]

Synonym: KP1_2947

Alternate gene names: 238894924

Gene position: 2813361-2814905 (Clockwise)

Preceding gene: 238894923

Following gene: 238894925

Centisome position: 53.6

GC content: 59.09

Gene sequence:

>1545_bases
ATGAAAATCCGTTCTCAGGTTGGAATGGTGCTGAATCTGGACAAATGCATCGGCTGTCACACCTGCTCGGTCACCTGTAA
AAACGTCTGGAGCAGCCGCGAAGGCATGGAGTACGCGTGGTTCAATAACGTGGAGACCAAACCGGGCATTGGCTACCCCA
AAAACTGGGAGGACCAGGATCAGTGGCAAGGCGGCTGGATCCGCGGCATTAGCGGTAAGCTGACGCCCCGCCTCGGCAAT
CGGGTCAGCGTATTATCGAAGATTTTCGCCAACCCGGTGCTGCCGGCGATCGACGATTACTACGAACCCTTTACCTATGA
CTACCAGCACCTGCACAACGCGCCGGAGGGCAAATATCTGCCGACCGCCCGCCCGCGCTCGCTGATCAGCGGCGAGCGTA
TGGATAAGATTAACTGGGGCCCGAACTGGGAGGAGCTGCTCGGCGGCGAATTCGAGAAACGCGCCCGCGACCGTAACTTC
GAAGCCATGCAAAAAGAGATGTACGGCCAGTTCGAAAATACCTTCATGATGTATCTCCCGCGCCTGTGCGAGCACTGCCT
CAACCCGAGCTGCGTGGCCACCTGCCCGAGCGGCGCCATCTACAAGCGTGAGGAAGATGGCATCGTACTGATCGATCAGG
ATAAATGCCGCGGCTGGCGGATGTGCATCAGCGGCTGCCCGTACAAAAAAATCTACTTTAACTGGAAGAGCGGTAAGTCG
GAAAAATGCATCTTCTGCTATCCGCGCATTGAGTCCGGGCAGCCAACGGTCTGCTCAGAAACCTGCGTCGGGCGCATCCG
CTATCTTGGCGTACTGCTGTATGACGCCGACCGCATTGAAGAGGCGGCCAGTACCGAACATGAAACTGACCTCTATGAGC
GCCAGTGCGAGGTGTTCCTTAATCCAAACGATCCGGCGGTCATCGAGGAAGCGCTAAAGCAAGGGATCCCGCACAACGTC
ATTGAGGCGGCGCAGAAGTCTCCGGTCTATAAGCTGGCGATGGACTGGAAGCTGGCTCTGCCGCTGCATCCGGAGTATCG
CACCCTGCCGATGGTCTGGTACGTGCCGCCCCTGTCGCCGATCCAGTCGGTTGCCGACGCCGGCGGCTTACCGAGCAACG
GCAACATCCTGCCGGCGGTTGAGAGTCTGCGCATCCCGGTGCAATACCTGGCGAATCTGCTGAGCGCCGGCGATACCGGA
CCGGTCTTGCGCGCGCTGAAACGGATGATGGCGATGCGCCACTACAAGCGCTCGCAAACCGTCGAGGGCGTCACCGATAC
CCGGGCCATTGAAGAGGTGGGCCTCAGCGTTGAGCAGGTCGAAGAGATGTATCGCTATCTGGCGATCGCCAACTACGAAG
ACCGCTTCGTGATCCCCACCAGCCATCGCGAACTGGCGGAGGATGCCTTTCCGGAACGTAACGGCTGCGGCTTTACCTTC
GGCGATGGCTGCCACGGGTCAGATACGAAATTCAACCTGTTTAACAGCCGGCGGATCGACGCCATCGACGTCAGCGGCGT
GCGTAAGCATGGGGAGGGCGAGTGA

Upstream 100 bases:

>100_bases
CCGTCGGATCGAACCGCGACGAGTTCATCATGATCCGCAAAATGAAGAAGATTGCCTGGCTGGATGACGAAGGTCGCGAT
CAGGTACAGGAGGCGAAAAA

Downstream 100 bases:

>100_bases
TGCGGATCCTGAAAGTGATCGGTCTGCTGCTGGAGTACCCCGACGAGTTACTGTGGGAGAACCGCGACGACGCCCTGGCG
CTGGTCAGGGCCGATGTCCC

Product: cryptic nitrate reductase 2 beta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWSSREGMEYAWFNNVETKPGIGYPKNWEDQDQWQGGWIRGISGKLTPRLGN
RVSVLSKIFANPVLPAIDDYYEPFTYDYQHLHNAPEGKYLPTARPRSLISGERMDKINWGPNWEELLGGEFEKRARDRNF
EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRGWRMCISGCPYKKIYFNWKSGKS
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEAASTEHETDLYERQCEVFLNPNDPAVIEEALKQGIPHNV
IEAAQKSPVYKLAMDWKLALPLHPEYRTLPMVWYVPPLSPIQSVADAGGLPSNGNILPAVESLRIPVQYLANLLSAGDTG
PVLRALKRMMAMRHYKRSQTVEGVTDTRAIEEVGLSVEQVEEMYRYLAIANYEDRFVIPTSHRELAEDAFPERNGCGFTF
GDGCHGSDTKFNLFNSRRIDAIDVSGVRKHGEGE

Sequences:

>Translated_514_residues
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWSSREGMEYAWFNNVETKPGIGYPKNWEDQDQWQGGWIRGISGKLTPRLGN
RVSVLSKIFANPVLPAIDDYYEPFTYDYQHLHNAPEGKYLPTARPRSLISGERMDKINWGPNWEELLGGEFEKRARDRNF
EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRGWRMCISGCPYKKIYFNWKSGKS
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEAASTEHETDLYERQCEVFLNPNDPAVIEEALKQGIPHNV
IEAAQKSPVYKLAMDWKLALPLHPEYRTLPMVWYVPPLSPIQSVADAGGLPSNGNILPAVESLRIPVQYLANLLSAGDTG
PVLRALKRMMAMRHYKRSQTVEGVTDTRAIEEVGLSVEQVEEMYRYLAIANYEDRFVIPTSHRELAEDAFPERNGCGFTF
GDGCHGSDTKFNLFNSRRIDAIDVSGVRKHGEGE
>Mature_514_residues
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWSSREGMEYAWFNNVETKPGIGYPKNWEDQDQWQGGWIRGISGKLTPRLGN
RVSVLSKIFANPVLPAIDDYYEPFTYDYQHLHNAPEGKYLPTARPRSLISGERMDKINWGPNWEELLGGEFEKRARDRNF
EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRGWRMCISGCPYKKIYFNWKSGKS
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEAASTEHETDLYERQCEVFLNPNDPAVIEEALKQGIPHNV
IEAAQKSPVYKLAMDWKLALPLHPEYRTLPMVWYVPPLSPIQSVADAGGLPSNGNILPAVESLRIPVQYLANLLSAGDTG
PVLRALKRMMAMRHYKRSQTVEGVTDTRAIEEVGLSVEQVEEMYRYLAIANYEDRFVIPTSHRELAEDAFPERNGCGFTF
GDGCHGSDTKFNLFNSRRIDAIDVSGVRKHGEGE

Specific function: This is a second nitrate reductase enzyme which can substitute for the NRA enzyme and allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in t

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787740, Length=514, Percent_Identity=88.5214007782101, Blast_Score=981, Evalue=0.0,
Organism=Escherichia coli, GI1787478, Length=506, Percent_Identity=82.4110671936759, Blast_Score=890, Evalue=0.0,
Organism=Escherichia coli, GI1787872, Length=99, Percent_Identity=42.4242424242424, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1787122, Length=101, Percent_Identity=41.5841584158416, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI2367345, Length=111, Percent_Identity=29.7297297297297, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1789370, Length=99, Percent_Identity=37.3737373737374, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI226510944, Length=86, Percent_Identity=36.046511627907, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1787749, Length=87, Percent_Identity=34.4827586206897, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790326, Length=87, Percent_Identity=33.3333333333333, Blast_Score=62, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 58440; Mature: 58440

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWSSREGMEYAWFNNVETKPGIGYPKNWEDQD
CCCHHHCCCEEEHHHHHCCCCHHEEHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCH
QWQGGWIRGISGKLTPRLGNRVSVLSKIFANPVLPAIDDYYEPFTYDYQHLHNAPEGKYL
HHCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHCCCCCCCCC
PTARPRSLISGERMDKINWGPNWEELLGGEFEKRARDRNFEAMQKEMYGQFENTFMMYLP
CCCCCHHHHCCCCCCCCCCCCCHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRGWRMCISGCPYKKIYFNWKSGKS
HHHHHHCCCCEEEECCCCCEEEECCCCEEEEECCCCCCHHHHHCCCCCEEEEEEECCCCC
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEAASTEHETDLYERQCEVFL
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHEECHHHHHHHHCCCHHHHHHHHCCEEEE
NPNDPAVIEEALKQGIPHNVIEAAQKSPVYKLAMDWKLALPLHPEYRTLPMVWYVPPLSP
CCCCCHHHHHHHHCCCCHHHHHHHHCCCCEEEEECEEEECCCCCCCCCCCEEEECCCCCH
IQSVADAGGLPSNGNILPAVESLRIPVQYLANLLSAGDTGPVLRALKRMMAMRHYKRSQT
HHHHHHCCCCCCCCCEECCHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCC
VEGVTDTRAIEEVGLSVEQVEEMYRYLAIANYEDRFVIPTSHRELAEDAFPERNGCGFTF
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHCCCCCCCCCCCC
GDGCHGSDTKFNLFNSRRIDAIDVSGVRKHGEGE
CCCCCCCCCEEEEECCCCCCEEECCCCCCCCCCC
>Mature Secondary Structure
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWSSREGMEYAWFNNVETKPGIGYPKNWEDQD
CCCHHHCCCEEEHHHHHCCCCHHEEHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCH
QWQGGWIRGISGKLTPRLGNRVSVLSKIFANPVLPAIDDYYEPFTYDYQHLHNAPEGKYL
HHCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHCCCCCCCCC
PTARPRSLISGERMDKINWGPNWEELLGGEFEKRARDRNFEAMQKEMYGQFENTFMMYLP
CCCCCHHHHCCCCCCCCCCCCCHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRGWRMCISGCPYKKIYFNWKSGKS
HHHHHHCCCCEEEECCCCCEEEECCCCEEEEECCCCCCHHHHHCCCCCEEEEEEECCCCC
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEAASTEHETDLYERQCEVFL
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHEECHHHHHHHHCCCHHHHHHHHCCEEEE
NPNDPAVIEEALKQGIPHNVIEAAQKSPVYKLAMDWKLALPLHPEYRTLPMVWYVPPLSP
CCCCCHHHHHHHHCCCCHHHHHHHHCCCCEEEEECEEEECCCCCCCCCCCEEEECCCCCH
IQSVADAGGLPSNGNILPAVESLRIPVQYLANLLSAGDTGPVLRALKRMMAMRHYKRSQT
HHHHHHCCCCCCCCCEECCHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCC
VEGVTDTRAIEEVGLSVEQVEEMYRYLAIANYEDRFVIPTSHRELAEDAFPERNGCGFTF
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHCCCCCCCCCCCC
GDGCHGSDTKFNLFNSRRIDAIDVSGVRKHGEGE
CCCCCCCCCEEEEECCCCCCEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2233673; 9097039; 9278503 [H]