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The map label for this gene is gatA2 [H]

Identifier: 238894806

GI number: 238894806

Start: 2687745

End: 2689142

Strand: Direct

Name: gatA2 [H]

Synonym: KP1_2819

Alternate gene names: 238894806

Gene position: 2687745-2689142 (Clockwise)

Preceding gene: 238894805

Following gene: 238894807

Centisome position: 51.21

GC content: 67.31

Gene sequence:

>1398_bases
ATGACTCTTCAACACTTCACCATTGCCGATCTCCAGCGTGCGCTGCACGATGGCGAACTGAGCGCCCGGGAGATCGCCCG
CCAGACGCTGGACGATATCGCCCGCGTGAATCCGCAGATCAACGCCTGGACCGAGGTCACCGCCCAGCGCATGCTGGCGG
AGGCCGACAGCATCGACGCCCTGCGCCGGGAAAAGCGCCCCCTGCCGCCGCTGGCAGGCATTCCTTACGCAGTGAAAAAT
CTGTTTGATGTCGCCGGGCACACCACCCTCGCCGGCGCCGAACTGCTGAGCGATCGGCCGCCGGCAGCCAGCGACAGCTG
GGCGGTGCGCCAGCTGCACAGCGCCGGCGCCCTGCTCAGCGGGATGCTCAATATGGACGCCTACGCCTACGGCTTCACCA
CCGAAAACAGCCACTATGGCGCGACGCGCAACCCGCACGATCTGTCGCGGATCGCCGGCGGTTCTTCCGGCGGTTCGGCC
GCGGCGGTGGCGGCCGGGCTGGTTCATTTCAGCCTCGGCAGCGACACCAACGGCTCGATCCGTGTCCCCGCGTCGCTGTG
CGGGATCTTTGGCCTGAAACCCACTTTCGGTCGCCTGTCGCGCTCTGGCAGCCATCCGTTTGTCGCCAGTCTGGATCATA
TTGGTCCTTTTGCCCGCCGGGTGGCCGACCTCGCCGCGGTGTATGACGCCCTGCAGGGGCGCGACCCGGCCGATGACTTT
CAGGCTGATAAAGCCAGCGAGCGCACCGGCAACATGCTGGAGCGCGGACTGGAGGGGCTGCGCTGCGCGCGGCTGGGGGG
CTATTTCACCACCTGGTGCGATGACGACGCCCGGGCGGCCGTGGAGCGGGTCGCCCACGCGCTGAGCGCCGACAGCGAGC
TGCAGTTCGCGGATGCCGCCCTGGCCCGCTCGGCGGCCTTTATCATCAGCGCCAGCGAAGGCGGCAATCAGTACCTGACG
GATCTTCGCCACTCGCCCGAGCGCTTTGAACCTCACTCCCGCGAGCGGCTGCTGGCCGGCGCGATGATCCCCTCCGCGTG
GTATCTGCAGGCTCAGCGTTTTCGCCGTCATGCTCGCCAGGCAATGAAATCCCTGTTCAGCCAGGCCGACGTGCTGATCG
CCCCGGCCACCCCATGCAGCGCCACGCCAATAGGCGCGGAGGAGATGGTGATTAACGGCCAGCCGCTGCCGGTGCGCGCC
AGCATGGGAATGCTGACGCAGCCGATCTCCTTCCTCGGTCTGCCGGTGGTGACCGTGCCCCTGCGTACCGCCAGCGGCAA
GCCGATTGGCCTGCAGCTGATTGCCGCGCCGTTTAACGAACAAGCCTGTCTTCGCGCCGCGCGGGCGCTGGAAGCGATGG
GCATTACCGATGCCCGAGTGGCGGAGAGTGCAGCATGA

Upstream 100 bases:

>100_bases
AGGAGCTGGAGCGCCAGTTTGCCCGCATCGCCGCCATGGCGCAGCCGCTGATGGACTATCCCCTCGGGCCGCGCGAGGAG
ATTGCGGGGGTGTATAAAGC

Downstream 100 bases:

>100_bases
TCAATCTGGATAATGTAGACCGCCCGGCGATCCTCGCCGAGGTGACGGCGGCGTTTTATCAGTATGAAGAGGCGCTGGTC
AGCAATAACATCGAGGCGCT

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A 2 [H]

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MTLQHFTIADLQRALHDGELSAREIARQTLDDIARVNPQINAWTEVTAQRMLAEADSIDALRREKRPLPPLAGIPYAVKN
LFDVAGHTTLAGAELLSDRPPAASDSWAVRQLHSAGALLSGMLNMDAYAYGFTTENSHYGATRNPHDLSRIAGGSSGGSA
AAVAAGLVHFSLGSDTNGSIRVPASLCGIFGLKPTFGRLSRSGSHPFVASLDHIGPFARRVADLAAVYDALQGRDPADDF
QADKASERTGNMLERGLEGLRCARLGGYFTTWCDDDARAAVERVAHALSADSELQFADAALARSAAFIISASEGGNQYLT
DLRHSPERFEPHSRERLLAGAMIPSAWYLQAQRFRRHARQAMKSLFSQADVLIAPATPCSATPIGAEEMVINGQPLPVRA
SMGMLTQPISFLGLPVVTVPLRTASGKPIGLQLIAAPFNEQACLRAARALEAMGITDARVAESAA

Sequences:

>Translated_465_residues
MTLQHFTIADLQRALHDGELSAREIARQTLDDIARVNPQINAWTEVTAQRMLAEADSIDALRREKRPLPPLAGIPYAVKN
LFDVAGHTTLAGAELLSDRPPAASDSWAVRQLHSAGALLSGMLNMDAYAYGFTTENSHYGATRNPHDLSRIAGGSSGGSA
AAVAAGLVHFSLGSDTNGSIRVPASLCGIFGLKPTFGRLSRSGSHPFVASLDHIGPFARRVADLAAVYDALQGRDPADDF
QADKASERTGNMLERGLEGLRCARLGGYFTTWCDDDARAAVERVAHALSADSELQFADAALARSAAFIISASEGGNQYLT
DLRHSPERFEPHSRERLLAGAMIPSAWYLQAQRFRRHARQAMKSLFSQADVLIAPATPCSATPIGAEEMVINGQPLPVRA
SMGMLTQPISFLGLPVVTVPLRTASGKPIGLQLIAAPFNEQACLRAARALEAMGITDARVAESAA
>Mature_464_residues
TLQHFTIADLQRALHDGELSAREIARQTLDDIARVNPQINAWTEVTAQRMLAEADSIDALRREKRPLPPLAGIPYAVKNL
FDVAGHTTLAGAELLSDRPPAASDSWAVRQLHSAGALLSGMLNMDAYAYGFTTENSHYGATRNPHDLSRIAGGSSGGSAA
AVAAGLVHFSLGSDTNGSIRVPASLCGIFGLKPTFGRLSRSGSHPFVASLDHIGPFARRVADLAAVYDALQGRDPADDFQ
ADKASERTGNMLERGLEGLRCARLGGYFTTWCDDDARAAVERVAHALSADSELQFADAALARSAAFIISASEGGNQYLTD
LRHSPERFEPHSRERLLAGAMIPSAWYLQAQRFRRHARQAMKSLFSQADVLIAPATPCSATPIGAEEMVINGQPLPVRAS
MGMLTQPISFLGLPVVTVPLRTASGKPIGLQLIAAPFNEQACLRAARALEAMGITDARVAESAA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=496, Percent_Identity=27.8225806451613, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI195972892, Length=235, Percent_Identity=30.6382978723404, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI166795287, Length=155, Percent_Identity=38.0645161290323, Blast_Score=72, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17543272, Length=446, Percent_Identity=28.0269058295964, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17556264, Length=218, Percent_Identity=37.1559633027523, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17537465, Length=487, Percent_Identity=24.0246406570842, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI71990152, Length=239, Percent_Identity=30.1255230125523, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17538252, Length=243, Percent_Identity=28.8065843621399, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17556276, Length=306, Percent_Identity=28.4313725490196, Blast_Score=82, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17556278, Length=170, Percent_Identity=35.8823529411765, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17538254, Length=222, Percent_Identity=28.8288288288288, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6319685, Length=429, Percent_Identity=29.6037296037296, Blast_Score=110, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6320448, Length=232, Percent_Identity=34.051724137931, Blast_Score=87, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6323950, Length=137, Percent_Identity=39.4160583941606, Blast_Score=83, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24648113, Length=498, Percent_Identity=32.1285140562249, Blast_Score=153, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24644968, Length=504, Percent_Identity=26.1904761904762, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24652985, Length=493, Percent_Identity=27.9918864097363, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI19922090, Length=493, Percent_Identity=27.9918864097363, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24652981, Length=493, Percent_Identity=27.9918864097363, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24652983, Length=493, Percent_Identity=27.9918864097363, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24648435, Length=486, Percent_Identity=26.5432098765432, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24648437, Length=486, Percent_Identity=26.5432098765432, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI45550774, Length=486, Percent_Identity=26.5432098765432, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI161078093, Length=505, Percent_Identity=26.7326732673267, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI21356731, Length=203, Percent_Identity=34.9753694581281, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24648441, Length=420, Percent_Identity=26.9047619047619, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24648439, Length=420, Percent_Identity=26.9047619047619, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 49599; Mature: 49467

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLQHFTIADLQRALHDGELSAREIARQTLDDIARVNPQINAWTEVTAQRMLAEADSIDA
CCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
LRREKRPLPPLAGIPYAVKNLFDVAGHTTLAGAELLSDRPPAASDSWAVRQLHSAGALLS
HHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
GMLNMDAYAYGFTTENSHYGATRNPHDLSRIAGGSSGGSAAAVAAGLVHFSLGSDTNGSI
HHHCCCCEEEEEECCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHEECCCCCCCCE
RVPASLCGIFGLKPTFGRLSRSGSHPFVASLDHIGPFARRVADLAAVYDALQGRDPADDF
ECCHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHH
QADKASERTGNMLERGLEGLRCARLGGYFTTWCDDDARAAVERVAHALSADSELQFADAA
HCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHH
LARSAAFIISASEGGNQYLTDLRHSPERFEPHSRERLLAGAMIPSAWYLQAQRFRRHARQ
HHHCEEEEEEECCCCHHHHHHHHCCCHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHH
AMKSLFSQADVLIAPATPCSATPIGAEEMVINGQPLPVRASMGMLTQPISFLGLPVVTVP
HHHHHHCCCCEEEECCCCCCCCCCCCHHEEECCCCCCCHHHCCHHHCCHHHHCCCEEEEE
LRTASGKPIGLQLIAAPFNEQACLRAARALEAMGITDARVAESAA
EECCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHCCC
>Mature Secondary Structure 
TLQHFTIADLQRALHDGELSAREIARQTLDDIARVNPQINAWTEVTAQRMLAEADSIDA
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
LRREKRPLPPLAGIPYAVKNLFDVAGHTTLAGAELLSDRPPAASDSWAVRQLHSAGALLS
HHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
GMLNMDAYAYGFTTENSHYGATRNPHDLSRIAGGSSGGSAAAVAAGLVHFSLGSDTNGSI
HHHCCCCEEEEEECCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHEECCCCCCCCE
RVPASLCGIFGLKPTFGRLSRSGSHPFVASLDHIGPFARRVADLAAVYDALQGRDPADDF
ECCHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHH
QADKASERTGNMLERGLEGLRCARLGGYFTTWCDDDARAAVERVAHALSADSELQFADAA
HCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHH
LARSAAFIISASEGGNQYLTDLRHSPERFEPHSRERLLAGAMIPSAWYLQAQRFRRHARQ
HHHCEEEEEEECCCCHHHHHHHHCCCHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHH
AMKSLFSQADVLIAPATPCSATPIGAEEMVINGQPLPVRASMGMLTQPISFLGLPVVTVP
HHHHHHCCCCEEEECCCCCCCCCCCCHHEEECCCCCCCHHHCCHHHCCHHHHCCCEEEEE
LRTASGKPIGLQLIAAPFNEQACLRAARALEAMGITDARVAESAA
EECCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]