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The map label for this gene is sgcA [H]

Identifier: 238894784

GI number: 238894784

Start: 2664580

End: 2665017

Strand: Reverse

Name: sgcA [H]

Synonym: KP1_2794

Alternate gene names: 238894784

Gene position: 2665017-2664580 (Counterclockwise)

Preceding gene: 238894787

Following gene: 238894783

Centisome position: 50.78

GC content: 45.89

Gene sequence:

>438_bases
ATGATCAGAGACTGGTTAACCCCGGATAAAATTAAAGTCATTAACTCAATAGATAGCTGGGAAAATGCCGTTCGACTTGC
AGCAGAACCCTTGCTGATGCAAGGGTATATAGCCGGGTCGTATATTGAGGCGATTTTGCAAAGCCATAAAGAGCTTGGGC
CCTATTACGTATTAGCTCCCGGCTTGGCGATGCCTCATGCCCGTCCTGAACAGGGGGTGTTAAGAAATGGATTATCGCTT
CTTCATATTAAAGAAGGGGTGTCGTTCGGCTCGGAAGAGAACGATCCGATATACGTGGTAATCATGCTATGCGCTCGTTC
CGGGAATGAACATATCACGATGATTGGTGAACTGGCTGAAATCTTTAGCGATCAGCAAAAGTTACACCGATTGTTAAATG
CCGATGATAGCAAGGCGATTCAGGCCGTTATTGACTGA

Upstream 100 bases:

>100_bases
TAACATTTTTCTTTGTTAGCGCTAACTTTGGGAGGTGTATCACAGTTGCATTTTATTTAGCTGTTTATAGTGCAGCACAT
AGACCCCCTCGGGAGAGCAT

Downstream 100 bases:

>100_bases
AGGAAAACAGAATGAAAAAAGTGCTTATCGTGTGCGGTAATGGTTTGGGCAGCAGCTTCATCGTTGAAATGAATGTCAAA
AAGATCCTTGCCGAAATGAA

Product: ascorbate-specific PTS family enzyme IIA component

Products: protein histidine; sugar phosphate

Alternate protein names: Putative PTS system EIIA component [H]

Number of amino acids: Translated: 145; Mature: 145

Protein sequence:

>145_residues
MIRDWLTPDKIKVINSIDSWENAVRLAAEPLLMQGYIAGSYIEAILQSHKELGPYYVLAPGLAMPHARPEQGVLRNGLSL
LHIKEGVSFGSEENDPIYVVIMLCARSGNEHITMIGELAEIFSDQQKLHRLLNADDSKAIQAVID

Sequences:

>Translated_145_residues
MIRDWLTPDKIKVINSIDSWENAVRLAAEPLLMQGYIAGSYIEAILQSHKELGPYYVLAPGLAMPHARPEQGVLRNGLSL
LHIKEGVSFGSEENDPIYVVIMLCARSGNEHITMIGELAEIFSDQQKLHRLLNADDSKAIQAVID
>Mature_145_residues
MIRDWLTPDKIKVINSIDSWENAVRLAAEPLLMQGYIAGSYIEAILQSHKELGPYYVLAPGLAMPHARPEQGVLRNGLSL
LHIKEGVSFGSEENDPIYVVIMLCARSGNEHITMIGELAEIFSDQQKLHRLLNADDSKAIQAVID

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1790755, Length=125, Percent_Identity=36, Blast_Score=102, Evalue=8e-24,
Organism=Escherichia coli, GI2367359, Length=146, Percent_Identity=33.5616438356164, Blast_Score=102, Evalue=1e-23,
Organism=Escherichia coli, GI1789302, Length=115, Percent_Identity=43.4782608695652, Blast_Score=94, Evalue=3e-21,
Organism=Escherichia coli, GI1790027, Length=113, Percent_Identity=38.9380530973451, Blast_Score=70, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 16108; Mature: 16108

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRDWLTPDKIKVINSIDSWENAVRLAAEPLLMQGYIAGSYIEAILQSHKELGPYYVLAP
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
GLAMPHARPEQGVLRNGLSLLHIKEGVSFGSEENDPIYVVIMLCARSGNEHITMIGELAE
CCCCCCCCCCHHHHHCCCCEEEECCCCCCCCCCCCCEEEEEEEECCCCCCEEHHHHHHHH
IFSDQQKLHRLLNADDSKAIQAVID
HHHHHHHHHHHHCCCCHHHHHHHCC
>Mature Secondary Structure
MIRDWLTPDKIKVINSIDSWENAVRLAAEPLLMQGYIAGSYIEAILQSHKELGPYYVLAP
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
GLAMPHARPEQGVLRNGLSLLHIKEGVSFGSEENDPIYVVIMLCARSGNEHITMIGELAE
CCCCCCCCCCHHHHHCCCCEEEECCCCCCCCCCCCCEEEEEEEECCCCCCEEHHHHHHHH
IFSDQQKLHRLLNADDSKAIQAVID
HHHHHHHHHHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]