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The map label for this gene is thlA [H]

Identifier: 238894738

GI number: 238894738

Start: 2620540

End: 2621730

Strand: Reverse

Name: thlA [H]

Synonym: KP1_2745

Alternate gene names: 238894738

Gene position: 2621730-2620540 (Counterclockwise)

Preceding gene: 238894739

Following gene: 238894737

Centisome position: 49.95

GC content: 62.8

Gene sequence:

>1191_bases
ATGACCCAGGCTAATGATATTGTGATTGTCAGCGGCGTCCGTACCGCTATTGGCACGTTTAACGGCAGTCTTAAACATAC
CCATCAGCATGACCTTGGGGCCGCCGTCATTCGCGAAGCGATCGCCCGCGCCGGGATTGCGCCGCAGGATATCGACGAAA
CGATCGTCGGCAACGTCGGGCAAATCGCCGAAAGCGGTTTTATCGCCCGCATCTGTCAGCTGCGCGCGGGGATCCCGCAG
GAGAGCACCGCCTATTCCGTTAACCGCCAGTGCGGCTCCGGACTTCAGGCCCTGGCGGACGGCATGATGCAGCTGCAAAG
CGGCCAGGCCGAGGTGGTCGTCGCCTGCGGCACTGAAAATATGACCCAGCTGCCCTACTACCTGCGTAAAGCGCGCGATG
GCTATCGCATGGGCCACGGCGAGCTGGAAGATGGCCTGATCTCCATTCTCACCTGGCCGGAAGGCCCTTATCACAACGGC
ATCACCGCGGAAAACGTCGCGCAACGCTTCGGTATTACCCGCGAAGCGATGGACGATTTCGCCTGGTCGAGCCAGCAGAA
GGCGCTGAAGGCGATCGCGGAAGGTCGCTTTCGCGAGCAGATCCTGGCCCTTGAGGTGCCGGACGGCAAAAAAGCGACCC
GCCTGTTTGCGACCGATGAACACCCGCGCAACACCCCGCGTGAAAAGCTCGCCACGCTGCGTCCGGCGTTTAAAGCCGAC
GGCGTGGTGACGGCGGCCAACTCATCGGGCATTAACGACGGCGCGGCGGCGCTGGTGATGATGACCCGCCAGCAGGCGGA
AAAACGCGGCTTAACCCCGCGCATGCGCATTCGCGGCTGGGCGGTCGCCGGCTGCGGGGCGGAGATTATGGGGTTTGGTC
CCTCTCCCGCCACCCGACGCCTGATGGACCGTCTCAATATAGATGTGCAGTCCATTGATTTAATTGAACTCAACGAGGCC
TTCGCCGCTCAGGCGCTGGCGGTGATGAACGATCTGCGTCTCGACCCGGCCCGCGTTAACGTCAACGGCGGCGCGATCGC
CCTCGGCCACCCGGTAGGCGCCAGCGGTGCGATCCTGCCGGTCAAACTGATGTATGAAATGGCGCGCAGCGGCGCACGCA
CTGGTCTGGTCACCATGTGCATCGGCGGCGGCCAGGGGATTTCTATGCTGTTCGAGCGCGAAGGCGCTTAG

Upstream 100 bases:

>100_bases
TCGGCGAGTGTGTGGCGTTTCTGGCGTCCGATGGCGCGCGCTATATCAACGGTGAAGTGATTAACGTCGGCGGCGGCATG
GTGCTGTAAGGAGAAAAACA

Downstream 100 bases:

>100_bases
CAAAAGACGGCCCGAAGTGGGGCTTCGGGCCGGGCGTCGTTCGGGTCAGCACCCGGCGACAACCTGTGAGCTGACGAACG
GGATCGTCTCGATCCCGGCA

Product: putative acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MTQANDIVIVSGVRTAIGTFNGSLKHTHQHDLGAAVIREAIARAGIAPQDIDETIVGNVGQIAESGFIARICQLRAGIPQ
ESTAYSVNRQCGSGLQALADGMMQLQSGQAEVVVACGTENMTQLPYYLRKARDGYRMGHGELEDGLISILTWPEGPYHNG
ITAENVAQRFGITREAMDDFAWSSQQKALKAIAEGRFREQILALEVPDGKKATRLFATDEHPRNTPREKLATLRPAFKAD
GVVTAANSSGINDGAAALVMMTRQQAEKRGLTPRMRIRGWAVAGCGAEIMGFGPSPATRRLMDRLNIDVQSIDLIELNEA
FAAQALAVMNDLRLDPARVNVNGGAIALGHPVGASGAILPVKLMYEMARSGARTGLVTMCIGGGQGISMLFEREGA

Sequences:

>Translated_396_residues
MTQANDIVIVSGVRTAIGTFNGSLKHTHQHDLGAAVIREAIARAGIAPQDIDETIVGNVGQIAESGFIARICQLRAGIPQ
ESTAYSVNRQCGSGLQALADGMMQLQSGQAEVVVACGTENMTQLPYYLRKARDGYRMGHGELEDGLISILTWPEGPYHNG
ITAENVAQRFGITREAMDDFAWSSQQKALKAIAEGRFREQILALEVPDGKKATRLFATDEHPRNTPREKLATLRPAFKAD
GVVTAANSSGINDGAAALVMMTRQQAEKRGLTPRMRIRGWAVAGCGAEIMGFGPSPATRRLMDRLNIDVQSIDLIELNEA
FAAQALAVMNDLRLDPARVNVNGGAIALGHPVGASGAILPVKLMYEMARSGARTGLVTMCIGGGQGISMLFEREGA
>Mature_395_residues
TQANDIVIVSGVRTAIGTFNGSLKHTHQHDLGAAVIREAIARAGIAPQDIDETIVGNVGQIAESGFIARICQLRAGIPQE
STAYSVNRQCGSGLQALADGMMQLQSGQAEVVVACGTENMTQLPYYLRKARDGYRMGHGELEDGLISILTWPEGPYHNGI
TAENVAQRFGITREAMDDFAWSSQQKALKAIAEGRFREQILALEVPDGKKATRLFATDEHPRNTPREKLATLRPAFKADG
VVTAANSSGINDGAAALVMMTRQQAEKRGLTPRMRIRGWAVAGCGAEIMGFGPSPATRRLMDRLNIDVQSIDLIELNEAF
AAQALAVMNDLRLDPARVNVNGGAIALGHPVGASGAILPVKLMYEMARSGARTGLVTMCIGGGQGISMLFEREGA

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=395, Percent_Identity=41.5189873417722, Blast_Score=289, Evalue=4e-78,
Organism=Homo sapiens, GI167614485, Length=389, Percent_Identity=40.1028277634961, Blast_Score=288, Evalue=6e-78,
Organism=Homo sapiens, GI4501853, Length=399, Percent_Identity=39.3483709273183, Blast_Score=248, Evalue=5e-66,
Organism=Homo sapiens, GI4557237, Length=391, Percent_Identity=34.2710997442455, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI4504327, Length=426, Percent_Identity=30.7511737089202, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI194353979, Length=396, Percent_Identity=30.5555555555556, Blast_Score=157, Evalue=1e-38,
Organism=Escherichia coli, GI1788554, Length=392, Percent_Identity=50.2551020408163, Blast_Score=338, Evalue=2e-94,
Organism=Escherichia coli, GI87082165, Length=392, Percent_Identity=43.8775510204082, Blast_Score=314, Evalue=8e-87,
Organism=Escherichia coli, GI1787663, Length=396, Percent_Identity=41.6666666666667, Blast_Score=278, Evalue=3e-76,
Organism=Escherichia coli, GI48994986, Length=396, Percent_Identity=39.3939393939394, Blast_Score=244, Evalue=5e-66,
Organism=Escherichia coli, GI1788683, Length=429, Percent_Identity=32.4009324009324, Blast_Score=179, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI133906874, Length=389, Percent_Identity=40.6169665809769, Blast_Score=301, Evalue=4e-82,
Organism=Caenorhabditis elegans, GI17535921, Length=392, Percent_Identity=33.9285714285714, Blast_Score=219, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI25147385, Length=388, Percent_Identity=31.1855670103093, Blast_Score=202, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI17551802, Length=427, Percent_Identity=29.03981264637, Blast_Score=167, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI17535917, Length=403, Percent_Identity=25.3101736972705, Blast_Score=125, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6325229, Length=397, Percent_Identity=37.7833753148615, Blast_Score=239, Evalue=8e-64,
Organism=Saccharomyces cerevisiae, GI6322031, Length=404, Percent_Identity=36.1386138613861, Blast_Score=215, Evalue=1e-56,
Organism=Drosophila melanogaster, GI24655093, Length=391, Percent_Identity=43.9897698209719, Blast_Score=327, Evalue=7e-90,
Organism=Drosophila melanogaster, GI17648125, Length=393, Percent_Identity=40.9669211195929, Blast_Score=272, Evalue=3e-73,
Organism=Drosophila melanogaster, GI24640423, Length=390, Percent_Identity=35.6410256410256, Blast_Score=233, Evalue=2e-61,
Organism=Drosophila melanogaster, GI17137578, Length=427, Percent_Identity=28.5714285714286, Blast_Score=152, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 42308; Mature: 42177

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQANDIVIVSGVRTAIGTFNGSLKHTHQHDLGAAVIREAIARAGIAPQDIDETIVGNVG
CCCCCCEEEEECHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCHH
QIAESGFIARICQLRAGIPQESTAYSVNRQCGSGLQALADGMMQLQSGQAEVVVACGTEN
HHHHHHHHHHHHHHHCCCCCCCHHEEHHHHHCCCHHHHHHHHHHHCCCCCEEEEEECCCC
MTQLPYYLRKARDGYRMGHGELEDGLISILTWPEGPYHNGITAENVAQRFGITREAMDDF
CHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCHHHHHHH
AWSSQQKALKAIAEGRFREQILALEVPDGKKATRLFATDEHPRNTPREKLATLRPAFKAD
HCCHHHHHHHHHHCCHHHHEEEEEECCCCCCCEEEEECCCCCCCCCHHHHHHHCCCHHCC
GVVTAANSSGINDGAAALVMMTRQQAEKRGLTPRMRIRGWAVAGCGAEIMGFGPSPATRR
CEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCEEECCCCHHHHH
LMDRLNIDVQSIDLIELNEAFAAQALAVMNDLRLDPARVNVNGGAIALGHPVGASGAILP
HHHHHCCCEEEEEEEECCHHHHHHHHHHHHHCCCCCEEEEECCCEEEECCCCCCCCCCHH
VKLMYEMARSGARTGLVTMCIGGGQGISMLFEREGA
HHHHHHHHHCCCCCCEEEEEECCCCCCEEHEECCCC
>Mature Secondary Structure 
TQANDIVIVSGVRTAIGTFNGSLKHTHQHDLGAAVIREAIARAGIAPQDIDETIVGNVG
CCCCCEEEEECHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCHH
QIAESGFIARICQLRAGIPQESTAYSVNRQCGSGLQALADGMMQLQSGQAEVVVACGTEN
HHHHHHHHHHHHHHHCCCCCCCHHEEHHHHHCCCHHHHHHHHHHHCCCCCEEEEEECCCC
MTQLPYYLRKARDGYRMGHGELEDGLISILTWPEGPYHNGITAENVAQRFGITREAMDDF
CHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCHHHHHHH
AWSSQQKALKAIAEGRFREQILALEVPDGKKATRLFATDEHPRNTPREKLATLRPAFKAD
HCCHHHHHHHHHHCCHHHHEEEEEECCCCCCCEEEEECCCCCCCCCHHHHHHHCCCHHCC
GVVTAANSSGINDGAAALVMMTRQQAEKRGLTPRMRIRGWAVAGCGAEIMGFGPSPATRR
CEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCEEECCCCHHHHH
LMDRLNIDVQSIDLIELNEAFAAQALAVMNDLRLDPARVNVNGGAIALGHPVGASGAILP
HHHHHCCCEEEEEEEECCHHHHHHHHHHHHHCCCCCEEEEECCCEEEECCCCCCCCCCHH
VKLMYEMARSGARTGLVTMCIGGGQGISMLFEREGA
HHHHHHHHHCCCCCCEEEEEECCCCCCEEHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]