Click here to switch to the map view.

The map label for this gene is fruB [H]

Identifier: 238894722

GI number: 238894722

Start: 2605119

End: 2605568

Strand: Reverse

Name: fruB [H]

Synonym: KP1_2729

Alternate gene names: 238894722

Gene position: 2605568-2605119 (Counterclockwise)

Preceding gene: 238894723

Following gene: 238894721

Centisome position: 49.64

GC content: 45.78

Gene sequence:

>450_bases
TTGGTTATGATCGATAACATCACGCTGGATAATCTGCATATTGGCTGCAAAGCCAGTAATAAAGCGGAAGTCATCGCCAT
GATCGGCGCGGAGTTTAAAGCCAAAGGCTATGTCAATCAGGAATGTGTCCACTTTCTGCTGGAGCGTGAACATCAGGTCT
CGACCTTTTTAGGCAATGGCATCACCCTGCCGCATTTGCCGAAATCCGCGACGGATATCATCCTTAAAACCGGGATTGAG
ATCTATCAGTTTCCTGATGGCGTAATCTGGGATCGGAGCAATGTAATGTTTATTGCCATCGGCGTGATCGCGAAAGAGAA
AGAGCATATTGACGTACTCAAGGAAATTGCCTCGATATTTAGCGATGAGCTGATCGCTAACGCACTGTCATTAATCTCCG
ACAAACATGATTTCCTGAAAATTCTGCAGCATAACGCCTTGCCCCATTAA

Upstream 100 bases:

>100_bases
TTTGCCCTGCCCGAGACCTCCAATAAATCGCTTGAGCAGTTAGAAGCAGAGCTGTCAGCAAACCATGAAGATAAAAAATC
CACTCGTCTCACCAGGGAGG

Downstream 100 bases:

>100_bases
GTATGACACCGACCGGAGCTCTGGCCCCGGTCGGATTTTCACCCCTCGCCCAGCAAACATGGCGAATAATCTGATTCTCC
CCGTTATAACCTCAATGGCA

Product: phosphoenolpyruvate-dependent PTS family enzyme IIA component

Products: protein histidine; sugar phosphate

Alternate protein names: MTP; Phosphotransferase FPr protein; Pseudo-HPr; Phosphocarrier protein HPr; Protein H; Fructose-specific phosphotransferase enzyme IIA component; PTS system fructose-specific EIIA component [H]

Number of amino acids: Translated: 149; Mature: 149

Protein sequence:

>149_residues
MVMIDNITLDNLHIGCKASNKAEVIAMIGAEFKAKGYVNQECVHFLLEREHQVSTFLGNGITLPHLPKSATDIILKTGIE
IYQFPDGVIWDRSNVMFIAIGVIAKEKEHIDVLKEIASIFSDELIANALSLISDKHDFLKILQHNALPH

Sequences:

>Translated_149_residues
MVMIDNITLDNLHIGCKASNKAEVIAMIGAEFKAKGYVNQECVHFLLEREHQVSTFLGNGITLPHLPKSATDIILKTGIE
IYQFPDGVIWDRSNVMFIAIGVIAKEKEHIDVLKEIASIFSDELIANALSLISDKHDFLKILQHNALPH
>Mature_149_residues
MVMIDNITLDNLHIGCKASNKAEVIAMIGAEFKAKGYVNQECVHFLLEREHQVSTFLGNGITLPHLPKSATDIILKTGIE
IYQFPDGVIWDRSNVMFIAIGVIAKEKEHIDVLKEIASIFSDELIANALSLISDKHDFLKILQHNALPH

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG4668

COG function: function code G; Mannitol/fructose-specific phosphotransferase system, IIA domain

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788494, Length=136, Percent_Identity=36.0294117647059, Blast_Score=112, Evalue=1e-26,
Organism=Escherichia coli, GI1790027, Length=135, Percent_Identity=32.5925925925926, Blast_Score=83, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR002114 [H]

Pfam domain/function: PF00381 PTS-HPr; PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 16617; Mature: 16617

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVMIDNITLDNLHIGCKASNKAEVIAMIGAEFKAKGYVNQECVHFLLEREHQVSTFLGNG
CEEECCEEECCEEEECCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCHHHHHHHCCC
ITLPHLPKSATDIILKTGIEIYQFPDGVIWDRSNVMFIAIGVIAKEKEHIDVLKEIASIF
CCCCCCCCHHHHHHHHCCCEEEECCCCEEECCCCEEEEEEEEHHCCHHHHHHHHHHHHHH
SDELIANALSLISDKHDFLKILQHNALPH
HHHHHHHHHHHHCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVMIDNITLDNLHIGCKASNKAEVIAMIGAEFKAKGYVNQECVHFLLEREHQVSTFLGNG
CEEECCEEECCEEEECCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCHHHHHHHCCC
ITLPHLPKSATDIILKTGIEIYQFPDGVIWDRSNVMFIAIGVIAKEKEHIDVLKEIASIF
CCCCCCCCHHHHHHHHCCCEEEECCCCEEECCCCEEEEEEEEHHCCHHHHHHHHHHHHHH
SDELIANALSLISDKHDFLKILQHNALPH
HHHHHHHHHHHHCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]