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The map label for this gene is yneJ [H]

Identifier: 238894689

GI number: 238894689

Start: 2574466

End: 2575311

Strand: Reverse

Name: yneJ [H]

Synonym: KP1_2695

Alternate gene names: 238894689

Gene position: 2575311-2574466 (Counterclockwise)

Preceding gene: 238894691

Following gene: 238894687

Centisome position: 49.07

GC content: 64.07

Gene sequence:

>846_bases
ATGAACCACAGCACACTCGAGATCTTCATTCAGGTGGCAGAAACGCAGAGCGTCACCCAGGCAGCAAAACGGCTGGGCCG
CGCACAGTCCAATATCACGACCCGCATTCAGCAGCTGGAAGAGGAGCTTGCCGTTGAGCTCTTCGTTCGCGGCAACAAAA
AAATGGTGTTGTCCCCGGCAGGCGTGCAGTTTCTCAGCTATGCCCGGCGGATCCTGAGCCTGGCGGAAGAGGCAAAGCAG
GCTCTGCATCCGACAACCCCAGGCGGCAGTCTGCGTCTGGGCGCGATGGAGGCGACCGCCGCCAGCCGGCTGCCGCCGCT
GCTTACCCGCTTCCAACAGCAGTGCCCGCAGGTGGATATCACCCTCATCACCCAACCAACCAGCCAGTTAACCGAGGGGG
TGTTAACCGCCGCCCTTGATGCCGCGCTGGTCTGCCTGCCGCCAGGGGCCGATGGGCAACCTGCCTGTCCCGACGAGCTG
GCATTCACCCCGGTGTTTTATGAAACCCTGATGCTGGTCCGTCCGCAGACCCCGGGTCCGCTGCGGTTCGCGGCATTTGC
CAGCGGCTGCAGCTACCGCGCGCTGGGAGAACGCTGGCTGGCGGAACAGCAGGCGGCCGTCGAGGTGCATGAGGTCAATT
CCTATCACAGCATGCTTGCCTGCGTGGCGTCCGGGCGCTACGCCTGCCTGCTGCCGCAGAGCGTGCTGTCGCTGATGACG
CTTCCCGAAAACTGCCTTAGCGAACCGCTGTGCGAGGCGACCACCCAGCTTATCTGGCGCAGCGGCTTGAGCGCCCCCGC
CCTGAGCGAGTGGCGCAAGCTGCTGCAAAGCGTGGCGATTGGCTAA

Upstream 100 bases:

>100_bases
GGGTTGCTCATGGTTTCCTCATGCATTAACGATGCCGGAGGCTTACCATCTCCTTTAAAGATGGTAAGATCAACATTATT
ATCGTATTGAGAAGAAAAAT

Downstream 100 bases:

>100_bases
GCCTGGCGTTCGGTAAAGACCAGCCCTGCGGCAATCAGCAGCGCCAGCGCCGCGGCATAGAGCGCCGGGTTAAATGAAGC
CGCGATCTGATACAGCGCGC

Product: putative LysR-family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MNHSTLEIFIQVAETQSVTQAAKRLGRAQSNITTRIQQLEEELAVELFVRGNKKMVLSPAGVQFLSYARRILSLAEEAKQ
ALHPTTPGGSLRLGAMEATAASRLPPLLTRFQQQCPQVDITLITQPTSQLTEGVLTAALDAALVCLPPGADGQPACPDEL
AFTPVFYETLMLVRPQTPGPLRFAAFASGCSYRALGERWLAEQQAAVEVHEVNSYHSMLACVASGRYACLLPQSVLSLMT
LPENCLSEPLCEATTQLIWRSGLSAPALSEWRKLLQSVAIG

Sequences:

>Translated_281_residues
MNHSTLEIFIQVAETQSVTQAAKRLGRAQSNITTRIQQLEEELAVELFVRGNKKMVLSPAGVQFLSYARRILSLAEEAKQ
ALHPTTPGGSLRLGAMEATAASRLPPLLTRFQQQCPQVDITLITQPTSQLTEGVLTAALDAALVCLPPGADGQPACPDEL
AFTPVFYETLMLVRPQTPGPLRFAAFASGCSYRALGERWLAEQQAAVEVHEVNSYHSMLACVASGRYACLLPQSVLSLMT
LPENCLSEPLCEATTQLIWRSGLSAPALSEWRKLLQSVAIG
>Mature_281_residues
MNHSTLEIFIQVAETQSVTQAAKRLGRAQSNITTRIQQLEEELAVELFVRGNKKMVLSPAGVQFLSYARRILSLAEEAKQ
ALHPTTPGGSLRLGAMEATAASRLPPLLTRFQQQCPQVDITLITQPTSQLTEGVLTAALDAALVCLPPGADGQPACPDEL
AFTPVFYETLMLVRPQTPGPLRFAAFASGCSYRALGERWLAEQQAAVEVHEVNSYHSMLACVASGRYACLLPQSVLSLMT
LPENCLSEPLCEATTQLIWRSGLSAPALSEWRKLLQSVAIG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787806, Length=290, Percent_Identity=39.3103448275862, Blast_Score=191, Evalue=6e-50,
Organism=Escherichia coli, GI1790208, Length=255, Percent_Identity=27.0588235294118, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI145693105, Length=285, Percent_Identity=25.2631578947368, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI87082132, Length=238, Percent_Identity=28.9915966386555, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788626, Length=150, Percent_Identity=28.6666666666667, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081904, Length=176, Percent_Identity=27.2727272727273, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788297, Length=248, Percent_Identity=27.4193548387097, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787879, Length=175, Percent_Identity=28.5714285714286, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1787601, Length=117, Percent_Identity=31.6239316239316, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI2367136, Length=117, Percent_Identity=30.7692307692308, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1786401, Length=124, Percent_Identity=30.6451612903226, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI157672245, Length=173, Percent_Identity=29.4797687861272, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1788481, Length=158, Percent_Identity=27.8481012658228, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1787589, Length=168, Percent_Identity=28.5714285714286, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 30444; Mature: 30444

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHSTLEIFIQVAETQSVTQAAKRLGRAQSNITTRIQQLEEELAVELFVRGNKKMVLSPA
CCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEECCC
GVQFLSYARRILSLAEEAKQALHPTTPGGSLRLGAMEATAASRLPPLLTRFQQQCPQVDI
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECHHHHHHHHCCCHHHHHHHHHCCCCEE
TLITQPTSQLTEGVLTAALDAALVCLPPGADGQPACPDELAFTPVFYETLMLVRPQTPGP
EEEECCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCHHCCHHHHHHHHHHHCCCCCCC
LRFAAFASGCSYRALGERWLAEQQAAVEVHEVNSYHSMLACVASGRYACLLPQSVLSLMT
CHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHH
LPENCLSEPLCEATTQLIWRSGLSAPALSEWRKLLQSVAIG
CCHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MNHSTLEIFIQVAETQSVTQAAKRLGRAQSNITTRIQQLEEELAVELFVRGNKKMVLSPA
CCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEECCC
GVQFLSYARRILSLAEEAKQALHPTTPGGSLRLGAMEATAASRLPPLLTRFQQQCPQVDI
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECHHHHHHHHCCCHHHHHHHHHCCCCEE
TLITQPTSQLTEGVLTAALDAALVCLPPGADGQPACPDELAFTPVFYETLMLVRPQTPGP
EEEECCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCHHCCHHHHHHHHHHHCCCCCCC
LRFAAFASGCSYRALGERWLAEQQAAVEVHEVNSYHSMLACVASGRYACLLPQSVLSLMT
CHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHH
LPENCLSEPLCEATTQLIWRSGLSAPALSEWRKLLQSVAIG
CCHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]