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The map label for this gene is amiC [H]

Identifier: 238894645

GI number: 238894645

Start: 2536849

End: 2538120

Strand: Direct

Name: amiC [H]

Synonym: KP1_2648

Alternate gene names: 238894645

Gene position: 2536849-2538120 (Clockwise)

Preceding gene: 238894644

Following gene: 238894646

Centisome position: 48.33

GC content: 58.57

Gene sequence:

>1272_bases
ATGCAACGTAGAACGTTATTAAAAGCTTTTGCACTCTCCGCCTCTGTTATGGCTATGGGTCTCTCCTTTCAGGCCTATGC
CGCCGACACCATCAAAGTCGGGATCATGCATTCGCTCTCCGGGACGATGGCGATTTCCGAAACGCCGCTGAAAGACGTCG
CGCTGATGGCCATCGACGACATCAACGCCAAAGGCGGGGTGCTGGGCAAGAAACTGGAGCCGGTGGTGGTCGACCCGGCC
TCCAACTGGCCGCTGTTTGCCGAGAAGGCGCGCCAGCTGCTGGCCCAGGATAAAGTGGCGGTGGTGTTTGGCTGCTGGAC
GTCGGTCTCGCGTAAATCGGTCCTGCCGGTGTTTGAGGAACTGAACGGCCTGCTGTTCTACCCGGTGCAGTACGAAGGGG
AAGAGATGTCCCCCAACGTCTTCTATACCGGCGCGGCGCCTAACCAGCAGGCTATCCCGGCGGTGGAATACCTGCTGAGC
GAGGACGGCGGCGGCGCTAAACGTTTCTTTCTGCTGGGGACGGACTACGTCTATCCGCGCACCACCAACAAGATCCTCCG
CGCCTTTCTGCATGCCAAAGGGATCCAGGACAAAGATATCGAGGAGGTCTACACCCCGTTCGGCTACAGCGATTACCAGA
CCATCGTCGCCAATATCAAAAAATTCGCCGCCGGTGGCAAAACGGCAGTGGTCTCCACCATCAATGGCGATTCCAACGTA
CCGTTCTACAAAGAGCTGGCTAACCAGGGTCTGAAAGCGACCGATGTACCGGTGGTGGCGTTCTCGGTGGGGGAAGAGGA
GCTGCGCGGCATCGATACCAAACCGCTGGTCGGCAACCTCGCGGCATGGAACTACTTTGAGTCCGTCGATAATCCGACCA
ACAAAGCCTTTGTCGCCGATTATCGCGCGTATGCCAAAGCGCACAAGCTGCCGAATGCCGATACGGTGGTGACCAACGAT
CCGATGGAAGCGACCTGGGTGGGCCTGCATATGTGGGCGCAGGCGGTGACCAAAGCGGGCACCACCGATGTTGACAAAGT
ACGCGAGGCGATGGCCGGGCAGACCTTCAACGCGCCGTCCGGATTTACCCTGACCATGGATGCCACCAACCACCATCTGC
ATAAGCCGGTGATGATCGGTGAAATTGAAGGCAACGGCCAGTTCAACGTGGTCTGGCAGACCGATGAGCCCGTTCGCGCG
CAGCCGTGGAGTCCGTGGATCCCCGGTAACGATAAGAAACCTGACCATCCGGTGAGAACCGTCAGCCAGTAA

Upstream 100 bases:

>100_bases
GCTCCGCATCCTGCTGTTTTCACAACCTGGCACCTTTTTTGCCTTATCACCTCTGGTTAGCTGTTAATTCTTTATTTTTT
GCGCTACTCAGGAGTGAATC

Downstream 100 bases:

>100_bases
GCGCCACGGCCGGCGGGGCTGGAGCATAGCGCCCGTCGGCCGCTGACCAGAGGAGAACGACATGAACGCATTGCGTCTCA
TGAATGTGTTGGCGCTATTG

Product: high-affinity branched-chain amino acid transporter periplasmic binding component

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MQRRTLLKAFALSASVMAMGLSFQAYAADTIKVGIMHSLSGTMAISETPLKDVALMAIDDINAKGGVLGKKLEPVVVDPA
SNWPLFAEKARQLLAQDKVAVVFGCWTSVSRKSVLPVFEELNGLLFYPVQYEGEEMSPNVFYTGAAPNQQAIPAVEYLLS
EDGGGAKRFFLLGTDYVYPRTTNKILRAFLHAKGIQDKDIEEVYTPFGYSDYQTIVANIKKFAAGGKTAVVSTINGDSNV
PFYKELANQGLKATDVPVVAFSVGEEELRGIDTKPLVGNLAAWNYFESVDNPTNKAFVADYRAYAKAHKLPNADTVVTND
PMEATWVGLHMWAQAVTKAGTTDVDKVREAMAGQTFNAPSGFTLTMDATNHHLHKPVMIGEIEGNGQFNVVWQTDEPVRA
QPWSPWIPGNDKKPDHPVRTVSQ

Sequences:

>Translated_423_residues
MQRRTLLKAFALSASVMAMGLSFQAYAADTIKVGIMHSLSGTMAISETPLKDVALMAIDDINAKGGVLGKKLEPVVVDPA
SNWPLFAEKARQLLAQDKVAVVFGCWTSVSRKSVLPVFEELNGLLFYPVQYEGEEMSPNVFYTGAAPNQQAIPAVEYLLS
EDGGGAKRFFLLGTDYVYPRTTNKILRAFLHAKGIQDKDIEEVYTPFGYSDYQTIVANIKKFAAGGKTAVVSTINGDSNV
PFYKELANQGLKATDVPVVAFSVGEEELRGIDTKPLVGNLAAWNYFESVDNPTNKAFVADYRAYAKAHKLPNADTVVTND
PMEATWVGLHMWAQAVTKAGTTDVDKVREAMAGQTFNAPSGFTLTMDATNHHLHKPVMIGEIEGNGQFNVVWQTDEPVRA
QPWSPWIPGNDKKPDHPVRTVSQ
>Mature_423_residues
MQRRTLLKAFALSASVMAMGLSFQAYAADTIKVGIMHSLSGTMAISETPLKDVALMAIDDINAKGGVLGKKLEPVVVDPA
SNWPLFAEKARQLLAQDKVAVVFGCWTSVSRKSVLPVFEELNGLLFYPVQYEGEEMSPNVFYTGAAPNQQAIPAVEYLLS
EDGGGAKRFFLLGTDYVYPRTTNKILRAFLHAKGIQDKDIEEVYTPFGYSDYQTIVANIKKFAAGGKTAVVSTINGDSNV
PFYKELANQGLKATDVPVVAFSVGEEELRGIDTKPLVGNLAAWNYFESVDNPTNKAFVADYRAYAKAHKLPNADTVVTND
PMEATWVGLHMWAQAVTKAGTTDVDKVREAMAGQTFNAPSGFTLTMDATNHHLHKPVMIGEIEGNGQFNVVWQTDEPVRA
QPWSPWIPGNDKKPDHPVRTVSQ

Specific function: Negatively regulates the expression of the aliphatic amidase operon. AmiC functions by inhibiting the action of AmiR at the protein level. It exhibits protein kinase activity [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000709 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 46240; Mature: 46240

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRRTLLKAFALSASVMAMGLSFQAYAADTIKVGIMHSLSGTMAISETPLKDVALMAIDD
CCHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEEEEECCCCCEEECCCCCHHEEEEEEEC
INAKGGVLGKKLEPVVVDPASNWPLFAEKARQLLAQDKVAVVFGCWTSVSRKSVLPVFEE
CCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHH
LNGLLFYPVQYEGEEMSPNVFYTGAAPNQQAIPAVEYLLSEDGGGAKRFFLLGTDYVYPR
CCCEEEEEEEECCCCCCCCEEEECCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCEECCC
TTNKILRAFLHAKGIQDKDIEEVYTPFGYSDYQTIVANIKKFAAGGKTAVVSTINGDSNV
CHHHHHHHHHHHCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
PFYKELANQGLKATDVPVVAFSVGEEELRGIDTKPLVGNLAAWNYFESVDNPTNKAFVAD
CHHHHHHHCCCCCCCCCEEEEECCHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCEEHHH
YRAYAKAHKLPNADTVVTNDPMEATWVGLHMWAQAVTKAGTTDVDKVREAMAGQTFNAPS
HHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCC
GFTLTMDATNHHLHKPVMIGEIEGNGQFNVVWQTDEPVRAQPWSPWIPGNDKKPDHPVRT
CEEEEEECCCCCCCCCEEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
VSQ
CCC
>Mature Secondary Structure
MQRRTLLKAFALSASVMAMGLSFQAYAADTIKVGIMHSLSGTMAISETPLKDVALMAIDD
CCHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEEEEECCCCCEEECCCCCHHEEEEEEEC
INAKGGVLGKKLEPVVVDPASNWPLFAEKARQLLAQDKVAVVFGCWTSVSRKSVLPVFEE
CCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHH
LNGLLFYPVQYEGEEMSPNVFYTGAAPNQQAIPAVEYLLSEDGGGAKRFFLLGTDYVYPR
CCCEEEEEEEECCCCCCCCEEEECCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCEECCC
TTNKILRAFLHAKGIQDKDIEEVYTPFGYSDYQTIVANIKKFAAGGKTAVVSTINGDSNV
CHHHHHHHHHHHCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
PFYKELANQGLKATDVPVVAFSVGEEELRGIDTKPLVGNLAAWNYFESVDNPTNKAFVAD
CHHHHHHHCCCCCCCCCEEEEECCHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCEEHHH
YRAYAKAHKLPNADTVVTNDPMEATWVGLHMWAQAVTKAGTTDVDKVREAMAGQTFNAPS
HHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCC
GFTLTMDATNHHLHKPVMIGEIEGNGQFNVVWQTDEPVRAQPWSPWIPGNDKKPDHPVRT
CEEEEEECCCCCCCCCEEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
VSQ
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1907262; 10984043; 1762155; 7813419; 10508151; 10708652 [H]