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The map label for this gene is lacI [H]

Identifier: 238894638

GI number: 238894638

Start: 2531866

End: 2532930

Strand: Direct

Name: lacI [H]

Synonym: KP1_2640

Alternate gene names: 238894638

Gene position: 2531866-2532930 (Clockwise)

Preceding gene: 238894635

Following gene: 238894640

Centisome position: 48.24

GC content: 67.32

Gene sequence:

>1065_bases
ATGCCGCGTCGTACCGCAACCCTGGAGGATGTCGCCCGCGCCGCGGGCGTGTCCCAGCAGACGGTCTCCCGGGTGCTGAA
CCGTCCTGAGGTGGTGTCAGCCCGCACCCGCGAGCAGGTGATCCGCGCGATGCAGGCGCTGCACTATGTGCCTAACCGCT
CGGCGCAGCTGCTTGCCGGCAAAGCGGCGCCCTCCATCGGGCTGATTACCGCCTCGCTGACGCTGCACGCCCCCTCGCAG
ATCGCCGCCGCGATAAAGAGCCATGCCAGCCTGCATCAGCTGGAAGTGGCGATAGCGATGCCGGCGCAGGCCGATTTTGT
CGCGCTGCAGGCCCGGCTGGACGAGCTGCGCGCCCAGCATATTCGCGGGGTGATCGTCAGTCTGCCGCTGGAGAGCGCCA
CTGCCGAGCGGCTGGTGCAAGACAATCCGGATATGGCCTGCCTGTTTCTCGATGTCTCCCCGGAGGCCGATGTCTGCTGC
GTGCGCTTCGACCACCGCGACGGCTGCGGGGCCTGCGTGCGCCACCTGTGGGAGCTGGGGCATCGCGAATTTGGTCTGCT
GGCGGGACCGGAAAGTTCGGTTTCCGCCCGTCTGCGTCTCGCCAGCTGGCGCGAGGCGTTGCACAGTCTGAATATTGCCC
GCTCTACTACGGTGTTTGGCGACTGGAGCGCCGCCAGCGGCTGGCAGAAAACTTTCGAGCTCCTCCATCTGCAGCCGCGG
ATCAGCGCCATAGTGGTGGCAAACGATCAGATGGCGCTCGGCGTGCTCAGCGCGCTGGCCCAGCTCAATCGCAGCGGCAG
CCGGGCAGTATCGGTGACCGGCTACGATGACACCGCCGACAGCCTTTACTTCCAGCCGCCGCTCACCACGGTGGCGCAGG
ACTTCGATCTGTTGGGCAAAAGGGCGGTGGAGCGGCTGATTGCCCTGATGGCGGCCCCGCAGCTGCGGATCCGCGAGCTG
CTGCCGACCCGGCTCATCGTGCGCCAGTCGGCCTGGCCTGTCGCCGCCGCGGAGGACCGGCAGCAGACCCTGGCGCAGCT
GAAAGCGCTGGTGGAGAAGCTTTAG

Upstream 100 bases:

>100_bases
ATTAACAGACGATCACGAAAGCGGGGGAGAAGCGCTGTTTTTTGTCGCGCGTTAAACATAAAATGTTAGCGCACGACATT
GTTATTGCTGAGGAGAAAAG

Downstream 100 bases:

>100_bases
CTGCTGGTCGTTGAGGCTTTATTGAAGTTGAGCATGCTAACTTCGGCAGCAAACGTAGAGAGGTAAAGCTTATCTGTCTG
CGAAAAATGCAAAACACAAT

Product: lac repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MPRRTATLEDVARAAGVSQQTVSRVLNRPEVVSARTREQVIRAMQALHYVPNRSAQLLAGKAAPSIGLITASLTLHAPSQ
IAAAIKSHASLHQLEVAIAMPAQADFVALQARLDELRAQHIRGVIVSLPLESATAERLVQDNPDMACLFLDVSPEADVCC
VRFDHRDGCGACVRHLWELGHREFGLLAGPESSVSARLRLASWREALHSLNIARSTTVFGDWSAASGWQKTFELLHLQPR
ISAIVVANDQMALGVLSALAQLNRSGSRAVSVTGYDDTADSLYFQPPLTTVAQDFDLLGKRAVERLIALMAAPQLRIREL
LPTRLIVRQSAWPVAAAEDRQQTLAQLKALVEKL

Sequences:

>Translated_354_residues
MPRRTATLEDVARAAGVSQQTVSRVLNRPEVVSARTREQVIRAMQALHYVPNRSAQLLAGKAAPSIGLITASLTLHAPSQ
IAAAIKSHASLHQLEVAIAMPAQADFVALQARLDELRAQHIRGVIVSLPLESATAERLVQDNPDMACLFLDVSPEADVCC
VRFDHRDGCGACVRHLWELGHREFGLLAGPESSVSARLRLASWREALHSLNIARSTTVFGDWSAASGWQKTFELLHLQPR
ISAIVVANDQMALGVLSALAQLNRSGSRAVSVTGYDDTADSLYFQPPLTTVAQDFDLLGKRAVERLIALMAAPQLRIREL
LPTRLIVRQSAWPVAAAEDRQQTLAQLKALVEKL
>Mature_353_residues
PRRTATLEDVARAAGVSQQTVSRVLNRPEVVSARTREQVIRAMQALHYVPNRSAQLLAGKAAPSIGLITASLTLHAPSQI
AAAIKSHASLHQLEVAIAMPAQADFVALQARLDELRAQHIRGVIVSLPLESATAERLVQDNPDMACLFLDVSPEADVCCV
RFDHRDGCGACVRHLWELGHREFGLLAGPESSVSARLRLASWREALHSLNIARSTTVFGDWSAASGWQKTFELLHLQPRI
SAIVVANDQMALGVLSALAQLNRSGSRAVSVTGYDDTADSLYFQPPLTTVAQDFDLLGKRAVERLIALMAAPQLRIRELL
PTRLIVRQSAWPVAAAEDRQQTLAQLKALVEKL

Specific function: Repressor of the lactose operon. Binds lactose as an inducer [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786540, Length=327, Percent_Identity=45.8715596330275, Blast_Score=264, Evalue=6e-72,
Organism=Escherichia coli, GI1790194, Length=329, Percent_Identity=27.6595744680851, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1787948, Length=333, Percent_Identity=29.1291291291291, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1788474, Length=301, Percent_Identity=31.5614617940199, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1790369, Length=334, Percent_Identity=28.4431137724551, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1789068, Length=332, Percent_Identity=29.2168674698795, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1789202, Length=288, Percent_Identity=30.2083333333333, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI1787580, Length=324, Percent_Identity=26.5432098765432, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1787906, Length=340, Percent_Identity=27.0588235294118, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1790715, Length=311, Percent_Identity=24.4372990353698, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790689, Length=302, Percent_Identity=25.8278145695364, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI48994940, Length=310, Percent_Identity=23.2258064516129, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 38551; Mature: 38420

Theoretical pI: Translated: 8.51; Mature: 8.51

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRRTATLEDVARAAGVSQQTVSRVLNRPEVVSARTREQVIRAMQALHYVPNRSAQLLAG
CCCCCCCHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEC
KAAPSIGLITASLTLHAPSQIAAAIKSHASLHQLEVAIAMPAQADFVALQARLDELRAQH
CCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHH
IRGVIVSLPLESATAERLVQDNPDMACLFLDVSPEADVCCVRFDHRDGCGACVRHLWELG
HCEEEEECCCCHHHHHHHHHCCCCEEEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHCC
HREFGLLAGPESSVSARLRLASWREALHSLNIARSTTVFGDWSAASGWQKTFELLHLQPR
CCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCHHHHHHHHHHCCCC
ISAIVVANDQMALGVLSALAQLNRSGSRAVSVTGYDDTADSLYFQPPLTTVAQDFDLLGK
CEEEEEECCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEECCCHHHHHHHHHHHHH
RAVERLIALMAAPQLRIRELLPTRLIVRQSAWPVAAAEDRQQTLAQLKALVEKL
HHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
PRRTATLEDVARAAGVSQQTVSRVLNRPEVVSARTREQVIRAMQALHYVPNRSAQLLAG
CCCCCCHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEC
KAAPSIGLITASLTLHAPSQIAAAIKSHASLHQLEVAIAMPAQADFVALQARLDELRAQH
CCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHH
IRGVIVSLPLESATAERLVQDNPDMACLFLDVSPEADVCCVRFDHRDGCGACVRHLWELG
HCEEEEECCCCHHHHHHHHHCCCCEEEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHCC
HREFGLLAGPESSVSARLRLASWREALHSLNIARSTTVFGDWSAASGWQKTFELLHLQPR
CCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCHHHHHHHHHHCCCC
ISAIVVANDQMALGVLSALAQLNRSGSRAVSVTGYDDTADSLYFQPPLTTVAQDFDLLGK
CEEEEEECCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEECCCHHHHHHHHHHHHH
RAVERLIALMAAPQLRIRELLPTRLIVRQSAWPVAAAEDRQQTLAQLKALVEKL
HHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3897196 [H]