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The map label for this gene is lacY [H]

Identifier: 238894636

GI number: 238894636

Start: 2527242

End: 2528513

Strand: Reverse

Name: lacY [H]

Synonym: KP1_2638

Alternate gene names: 238894636

Gene position: 2528513-2527242 (Counterclockwise)

Preceding gene: 238894637

Following gene: 238894633

Centisome position: 48.18

GC content: 56.13

Gene sequence:

>1272_bases
ATGGTGATGAAATTCTCAGAACTGGCGCCACGAGAACGGCATAATTTCGTCTATTTCCTGCTGTTCTTTTTCTTTTACTA
TTTCATTATGTCGGCCTACTTCCCGTTTTTTCCGGTGTGGCTGGCGGACGTTAACCATTTAACTAAAACGGAAACCGGGA
TCGTTTTCTCGTCTATCTCGTTATTCGCCATTATTTTTCAGCCGGTGTTCGGCCTGATGTCGGATAAGCTCGGCCTGCGC
AAACATCTGCTGTGGACCATTACGGTATTGTTAATTCTGTTCGCGCCATTCTTTATTTTCGTTTTCTCTCCGCTGCTGCA
GATGAATATTATCGCTGGTTCGCTGGTAGGCGGGATCTACCTGGGGATTGTTTTCTCCAGCGGCTCCGGGGCGGTGGAAG
CTTATATCGAACGCGTCAGCCGGGCCAACCGCTTTGAATATGGCAAAGTGCGGGTCGCAGGCTGCGTCGGCTGGGCGCTA
TGCGCCTCGATAACCGGCGTGCTGTTCGGCATCGATCCCAATATCACCTTCTGGATCGCCTCCGGCTTTGCGCTGGTGCT
CGGTCTGCTGCTCTGGCTGTCGCGGCCGGAAAGCAGCAACAGCGCCCAGGTTATCGAGGCGCTGGGCGCCAATCGCCAGG
CCTTTTCGCTGCGTACGGCGGCAGAGCTGCTGCGTATGCCGCGCTTCTGGGGCTTTATCGTCTATGTGGTGGGTGTCGCC
AGCGTCTACGACGTGTTTGACCAGCAGTTCGCCAACTTTTTCAAAAGCTTTTTCGTCAGTCCGCAGCGCGGTACCGAAGT
GTTTGGCTTCGTCACCACCGGCGGCGAGTTGCTCAACGCGCTGATCATGTTCTGCGCGCCGGCCATCGTTAACCGCATCG
GCGCCAAAAACGCCCTGCTGACCGCGGGGATGATCATGTCGGTGCGTATTCTCGGGTCGTCCTTCGCCTCATCGGCAGTC
GAGGTGGTGATCCTCAAGATGCTGCATATGTTTGAGATCCCCTTCCTGCTGGTGGGAACTTTTAAATATATCTCTTCCGC
CTTTAACCCGCGCCTCTCGGCCACCCTGTTCCTGATCGGTTTTAATCTGTCAAAACAGTTGTCCGGGGTGGTGCTTTCCG
CCTGGGTGGGCCGGATGTACGACACCGTCGGCTTCCATCAGGCCTATCTGATCCTCGGCTGCATAACCCTGAGCTTCACC
CTGCTCTCGTTCGTCACCCTACGCGGCGGCAACCGTCTGCTGCCGACCGCAGAGACGCAGAGCCCCGCCTGA

Upstream 100 bases:

>100_bases
GCTCCTGAGCCAAACGCGCTGGCAGTACGAGGTCTCATTGCGTTGCTTTTAATCCGTGGGGGCGACAGCCCCCACCCCAC
AAACAGAATAACAAGGGATC

Downstream 100 bases:

>100_bases
CTCCAGCGCCCCCGTCAGGGATGACGGGCTTCACTCGACAATCCGCGTCTCACCGCCGCGATTCTCCAGCGCATAGCGCT
GACAAGGCGCCCCGGCGGCG

Product: putative proton/sugar symporter

Products: Proton [Cytoplasm]; lactose [Cytoplasm] [C]

Alternate protein names: Lactose-proton symport [H]

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MVMKFSELAPRERHNFVYFLLFFFFYYFIMSAYFPFFPVWLADVNHLTKTETGIVFSSISLFAIIFQPVFGLMSDKLGLR
KHLLWTITVLLILFAPFFIFVFSPLLQMNIIAGSLVGGIYLGIVFSSGSGAVEAYIERVSRANRFEYGKVRVAGCVGWAL
CASITGVLFGIDPNITFWIASGFALVLGLLLWLSRPESSNSAQVIEALGANRQAFSLRTAAELLRMPRFWGFIVYVVGVA
SVYDVFDQQFANFFKSFFVSPQRGTEVFGFVTTGGELLNALIMFCAPAIVNRIGAKNALLTAGMIMSVRILGSSFASSAV
EVVILKMLHMFEIPFLLVGTFKYISSAFNPRLSATLFLIGFNLSKQLSGVVLSAWVGRMYDTVGFHQAYLILGCITLSFT
LLSFVTLRGGNRLLPTAETQSPA

Sequences:

>Translated_423_residues
MVMKFSELAPRERHNFVYFLLFFFFYYFIMSAYFPFFPVWLADVNHLTKTETGIVFSSISLFAIIFQPVFGLMSDKLGLR
KHLLWTITVLLILFAPFFIFVFSPLLQMNIIAGSLVGGIYLGIVFSSGSGAVEAYIERVSRANRFEYGKVRVAGCVGWAL
CASITGVLFGIDPNITFWIASGFALVLGLLLWLSRPESSNSAQVIEALGANRQAFSLRTAAELLRMPRFWGFIVYVVGVA
SVYDVFDQQFANFFKSFFVSPQRGTEVFGFVTTGGELLNALIMFCAPAIVNRIGAKNALLTAGMIMSVRILGSSFASSAV
EVVILKMLHMFEIPFLLVGTFKYISSAFNPRLSATLFLIGFNLSKQLSGVVLSAWVGRMYDTVGFHQAYLILGCITLSFT
LLSFVTLRGGNRLLPTAETQSPA
>Mature_423_residues
MVMKFSELAPRERHNFVYFLLFFFFYYFIMSAYFPFFPVWLADVNHLTKTETGIVFSSISLFAIIFQPVFGLMSDKLGLR
KHLLWTITVLLILFAPFFIFVFSPLLQMNIIAGSLVGGIYLGIVFSSGSGAVEAYIERVSRANRFEYGKVRVAGCVGWAL
CASITGVLFGIDPNITFWIASGFALVLGLLLWLSRPESSNSAQVIEALGANRQAFSLRTAAELLRMPRFWGFIVYVVGVA
SVYDVFDQQFANFFKSFFVSPQRGTEVFGFVTTGGELLNALIMFCAPAIVNRIGAKNALLTAGMIMSVRILGSSFASSAV
EVVILKMLHMFEIPFLLVGTFKYISSAFNPRLSATLFLIGFNLSKQLSGVVLSAWVGRMYDTVGFHQAYLILGCITLSFT
LLSFVTLRGGNRLLPTAETQSPA

Specific function: Responsible for transport of beta-galactosides into the cell, with the concomitant import of a proton (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lacY/rafB permease family [H]

Homologues:

Organism=Escherichia coli, GI1786538, Length=381, Percent_Identity=61.6797900262467, Blast_Score=493, Evalue=1e-140,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022814
- InterPro:   IPR000576
- InterPro:   IPR018457
- InterPro:   IPR020846
- InterPro:   IPR016196 [H]

Pfam domain/function: PF01306 LacY_symp [H]

EC number: NA

Molecular weight: Translated: 46881; Mature: 46881

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: PS50850 MFS ; PS00896 LACY_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVMKFSELAPRERHNFVYFLLFFFFYYFIMSAYFPFFPVWLADVNHLTKTETGIVFSSIS
CCCCCHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LFAIIFQPVFGLMSDKLGLRKHLLWTITVLLILFAPFFIFVFSPLLQMNIIAGSLVGGIY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGIVFSSGSGAVEAYIERVSRANRFEYGKVRVAGCVGWALCASITGVLFGIDPNITFWIA
HHHEEECCCCHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHEEECCCCEEEEHH
SGFALVLGLLLWLSRPESSNSAQVIEALGANRQAFSLRTAAELLRMPRFWGFIVYVVGVA
HHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHH
SVYDVFDQQFANFFKSFFVSPQRGTEVFGFVTTGGELLNALIMFCAPAIVNRIGAKNALL
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHH
TAGMIMSVRILGSSFASSAVEVVILKMLHMFEIPFLLVGTFKYISSAFNPRLSATLFLIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEE
FNLSKQLSGVVLSAWVGRMYDTVGFHQAYLILGCITLSFTLLSFVTLRGGNRLLPTAETQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCC
SPA
CCC
>Mature Secondary Structure
MVMKFSELAPRERHNFVYFLLFFFFYYFIMSAYFPFFPVWLADVNHLTKTETGIVFSSIS
CCCCCHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LFAIIFQPVFGLMSDKLGLRKHLLWTITVLLILFAPFFIFVFSPLLQMNIIAGSLVGGIY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGIVFSSGSGAVEAYIERVSRANRFEYGKVRVAGCVGWALCASITGVLFGIDPNITFWIA
HHHEEECCCCHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHEEECCCCEEEEHH
SGFALVLGLLLWLSRPESSNSAQVIEALGANRQAFSLRTAAELLRMPRFWGFIVYVVGVA
HHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHH
SVYDVFDQQFANFFKSFFVSPQRGTEVFGFVTTGGELLNALIMFCAPAIVNRIGAKNALL
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHH
TAGMIMSVRILGSSFASSAVEVVILKMLHMFEIPFLLVGTFKYISSAFNPRLSATLFLIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEE
FNLSKQLSGVVLSAWVGRMYDTVGFHQAYLILGCITLSFTLLSFVTLRGGNRLLPTAETQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCC
SPA
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; lactose [Periplasm] [C]

Specific reaction: Proton [Periplasm] + lactose [Periplasm] = Proton [Cytoplasm] + lactose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3056524 [H]