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The map label for this gene is ycgF [H]

Identifier: 238894623

GI number: 238894623

Start: 2514922

End: 2516190

Strand: Reverse

Name: ycgF [H]

Synonym: KP1_2624

Alternate gene names: 238894623

Gene position: 2516190-2514922 (Counterclockwise)

Preceding gene: 238894625

Following gene: 238894619

Centisome position: 47.94

GC content: 59.02

Gene sequence:

>1269_bases
TTGCATGCTGAGCGCGGATGTTCTTACGCCGCGGGAGCGATCGTAACAACTATGCTAACCACCCTGATTTACCGCAGCCA
GGTTCATCCTGACCGCCCGCCCGTCGATCTCGACGCTTTAGTCCATCGCGCCAGCAGTAAAAATTTGCCCCTTGGCATCA
CCGGCATTCTGCTGTTTAACGGCCTGCAGTTTTTCCAGGTCCTGGAAGGGACTGAGGAGGCACTTGAGTCGCTGTTCAGC
GAAATCCAAAGCGACCCGCGTCATCGCGACGTAGTGGAGCTGATGCGCGATTACTCAGCCTACCGACGTTTTCACGGCAC
CGGAATGCGCATACTGGATCTCCGGCTGTTTGAAACCGATGGCGCGCTGGAGGAAATTCTGCGCTTTAGCACGTTTGGGG
TCACCGAACCGGTCAACGACCGGATGTTCCGGCTGCTTAGCGCCTTCATTGCCGACGGCGGCCGCTATTGCCTGCCCGAG
CCGCTGCAGCCCTCTCGCTGGATGATGATGCCCGCGTCAGGTACGGCCGCGCCGCAGCACCTGCCCGGTCAACCTTGCCA
GTTTGCTCTGCAGGCTATTGTCGAGCCGGCTAAAAAGCGAGTTTCTTCCTTTGAGGCTCTGATCCGCAGCCCAACCGGCG
GCTCTCCCGTGGAAATGTTTGCCGCCATTGCGGCGGAGGATCGCTACCGTTTTGATCTTGAAAGCAAAGCGTTTGCATTC
GCTCTCGCCGGCCAGCTGCCGCTCGGGAAACATCAGCTGGCGATTAACCTGTTGCCCGGATCGCTGTATCACCACCCTGA
CGCCGTAGGCTGGCTGATGGACAGCCTGCTGGCGGCCGGCCTCAGACCGGATCAGGTACTGATCGAGGTGACCGAGACAG
AGGTGATCACCTGCTTTGACCAGTTCCGCAAGGTGCTCAAAGCCCTGCGCGTCGCGGGCATGAAGCTGGCCATCGACGAC
TTTGGCGCAGGTTATTCCGGCCTGTCGTTACTGACCCGTTTTCAGCCTGATAAAATCAAAGTGGATGCGGAACTGGTGCG
CGATATCCATATTAGCGGCACCAAACAGGCGATTGTCGCCTCGGTCGTCCGCTGCTGCGAGGATCTTGGCATTACGGTGG
TGGCTGAAGGTGTCGAAACCCTGGAGGAGTGGTGCTGGCTGCAGTCGGTGGGCATACGCCTGTTTCAGGGCTTTCTCTTT
TCCCGACCCTGCCTTAACGGCATCGGGGAGATTTGCTGGCCGGTAGCGCGCCAGGCAATGGACCTGTAA

Upstream 100 bases:

>100_bases
TCACAAAGTGAAGCTCGCGTTCCGCGTAGCGGACATCACGGTTGGTTGAATGTTTGTACATATCAGCCTCGCCCTCCTTT
AGGGTCAGGCCATACTCATC

Downstream 100 bases:

>100_bases
GGCCGCGCCCCCGGGCATCGTCACTGCCAGTGGGCAAGCTTTTCGCTCAGCGCCGCCAGGGCATATCGTACCGCTTTCGC
CACCACGGTCTCACAGTCGC

Product: hypothetical protein

Products: NA

Alternate protein names: Blrp [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MHAERGCSYAAGAIVTTMLTTLIYRSQVHPDRPPVDLDALVHRASSKNLPLGITGILLFNGLQFFQVLEGTEEALESLFS
EIQSDPRHRDVVELMRDYSAYRRFHGTGMRILDLRLFETDGALEEILRFSTFGVTEPVNDRMFRLLSAFIADGGRYCLPE
PLQPSRWMMMPASGTAAPQHLPGQPCQFALQAIVEPAKKRVSSFEALIRSPTGGSPVEMFAAIAAEDRYRFDLESKAFAF
ALAGQLPLGKHQLAINLLPGSLYHHPDAVGWLMDSLLAAGLRPDQVLIEVTETEVITCFDQFRKVLKALRVAGMKLAIDD
FGAGYSGLSLLTRFQPDKIKVDAELVRDIHISGTKQAIVASVVRCCEDLGITVVAEGVETLEEWCWLQSVGIRLFQGFLF
SRPCLNGIGEICWPVARQAMDL

Sequences:

>Translated_422_residues
MHAERGCSYAAGAIVTTMLTTLIYRSQVHPDRPPVDLDALVHRASSKNLPLGITGILLFNGLQFFQVLEGTEEALESLFS
EIQSDPRHRDVVELMRDYSAYRRFHGTGMRILDLRLFETDGALEEILRFSTFGVTEPVNDRMFRLLSAFIADGGRYCLPE
PLQPSRWMMMPASGTAAPQHLPGQPCQFALQAIVEPAKKRVSSFEALIRSPTGGSPVEMFAAIAAEDRYRFDLESKAFAF
ALAGQLPLGKHQLAINLLPGSLYHHPDAVGWLMDSLLAAGLRPDQVLIEVTETEVITCFDQFRKVLKALRVAGMKLAIDD
FGAGYSGLSLLTRFQPDKIKVDAELVRDIHISGTKQAIVASVVRCCEDLGITVVAEGVETLEEWCWLQSVGIRLFQGFLF
SRPCLNGIGEICWPVARQAMDL
>Mature_422_residues
MHAERGCSYAAGAIVTTMLTTLIYRSQVHPDRPPVDLDALVHRASSKNLPLGITGILLFNGLQFFQVLEGTEEALESLFS
EIQSDPRHRDVVELMRDYSAYRRFHGTGMRILDLRLFETDGALEEILRFSTFGVTEPVNDRMFRLLSAFIADGGRYCLPE
PLQPSRWMMMPASGTAAPQHLPGQPCQFALQAIVEPAKKRVSSFEALIRSPTGGSPVEMFAAIAAEDRYRFDLESKAFAF
ALAGQLPLGKHQLAINLLPGSLYHHPDAVGWLMDSLLAAGLRPDQVLIEVTETEVITCFDQFRKVLKALRVAGMKLAIDD
FGAGYSGLSLLTRFQPDKIKVDAELVRDIHISGTKQAIVASVVRCCEDLGITVVAEGVETLEEWCWLQSVGIRLFQGFLF
SRPCLNGIGEICWPVARQAMDL

Specific function: Binds to and releases the YcgE repressor from its bound DNA target in a blue-light-dependent (470 nm) fashion. A shift to low temperature also triggers a YcgF-medisted relief of repression by YcgE, suggesting YcgF may serve as a thermometer. Blue light ma

COG id: COG2200

COG function: function code T; FOG: EAL domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1787410, Length=405, Percent_Identity=41.7283950617284, Blast_Score=325, Evalue=3e-90,
Organism=Escherichia coli, GI1790496, Length=150, Percent_Identity=33.3333333333333, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1787541, Length=116, Percent_Identity=40.5172413793103, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI226510982, Length=161, Percent_Identity=32.2981366459627, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788502, Length=226, Percent_Identity=30.0884955752212, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87081921, Length=130, Percent_Identity=35.3846153846154, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87082096, Length=176, Percent_Identity=30.1136363636364, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI87081845, Length=164, Percent_Identity=33.5365853658537, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788849, Length=155, Percent_Identity=34.1935483870968, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87081980, Length=120, Percent_Identity=32.5, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1787055, Length=151, Percent_Identity=32.4503311258278, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1786507, Length=119, Percent_Identity=31.9327731092437, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI87081743, Length=131, Percent_Identity=29.7709923664122, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR007024
- InterPro:   IPR001633 [H]

Pfam domain/function: PF04940 BLUF; PF00563 EAL [H]

EC number: NA

Molecular weight: Translated: 46727; Mature: 46727

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS50925 BLUF ; PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHAERGCSYAAGAIVTTMLTTLIYRSQVHPDRPPVDLDALVHRASSKNLPLGITGILLFN
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHH
GLQFFQVLEGTEEALESLFSEIQSDPRHRDVVELMRDYSAYRRFHGTGMRILDLRLFETD
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCC
GALEEILRFSTFGVTEPVNDRMFRLLSAFIADGGRYCLPEPLQPSRWMMMPASGTAAPQH
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCC
LPGQPCQFALQAIVEPAKKRVSSFEALIRSPTGGSPVEMFAAIAAEDRYRFDLESKAFAF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEECCCCHHHHH
ALAGQLPLGKHQLAINLLPGSLYHHPDAVGWLMDSLLAAGLRPDQVLIEVTETEVITCFD
HHHCCCCCCCCEEEEEECCCCHHCCCHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHH
QFRKVLKALRVAGMKLAIDDFGAGYSGLSLLTRFQPDKIKVDAELVRDIHISGTKQAIVA
HHHHHHHHHHHCCCEEEEHHCCCCHHHHHHHHHCCCCCEEECHHHHHHHCCCCHHHHHHH
SVVRCCEDLGITVVAEGVETLEEWCWLQSVGIRLFQGFLFSRPCLNGIGEICWPVARQAM
HHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHC
DL
CC
>Mature Secondary Structure
MHAERGCSYAAGAIVTTMLTTLIYRSQVHPDRPPVDLDALVHRASSKNLPLGITGILLFN
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHH
GLQFFQVLEGTEEALESLFSEIQSDPRHRDVVELMRDYSAYRRFHGTGMRILDLRLFETD
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCC
GALEEILRFSTFGVTEPVNDRMFRLLSAFIADGGRYCLPEPLQPSRWMMMPASGTAAPQH
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCC
LPGQPCQFALQAIVEPAKKRVSSFEALIRSPTGGSPVEMFAAIAAEDRYRFDLESKAFAF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEECCCCHHHHH
ALAGQLPLGKHQLAINLLPGSLYHHPDAVGWLMDSLLAAGLRPDQVLIEVTETEVITCFD
HHHCCCCCCCCEEEEEECCCCHHCCCHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHH
QFRKVLKALRVAGMKLAIDDFGAGYSGLSLLTRFQPDKIKVDAELVRDIHISGTKQAIVA
HHHHHHHHHHHCCCEEEEHHCCCCHHHHHHHHHCCCCCEEECHHHHHHHCCCCHHHHHHH
SVVRCCEDLGITVVAEGVETLEEWCWLQSVGIRLFQGFLFSRPCLNGIGEICWPVARQAM
HHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHC
DL
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]