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The map label for this gene is ebgA [C]

Identifier: 238894392

GI number: 238894392

Start: 2277559

End: 2279757

Strand: Reverse

Name: ebgA [C]

Synonym: KP1_2375

Alternate gene names: 238894392

Gene position: 2279757-2277559 (Counterclockwise)

Preceding gene: 238894393

Following gene: 238894388

Centisome position: 43.44

GC content: 63.12

Gene sequence:

>2199_bases
ATGATCGCTAGACGTCTTCTGCTGCTGGTCATTTGCCTGCCGCTGGCGCACGCCGGTTCTGCGCCGGTGACCTGGTCACT
GGCCGGACAATGGCGGGTGCATGACGCCAACGACAGCGCCTTCGCCGGCGCGCAGGCTTCGGACGGCGACTGGCGCACGC
TGCGGGTACCGGCAAACTGGTACAGCGCCGGATACGATCATCAGGGCGCCCTGTGGTATCGCCATCAATTTACCCTGCAT
GGCCTGACGCCGGACGCCATGGCTACGCTGGTGTTTGACGGCGTCGATTACTTTGCCGAGGTGGCGCTCAACGGCAAGAC
GCTGACCCGGCACGAAGGCTATTTTCAGCGCTTCCCGGTGGACATCACCGGGGCCGTTCGCCGCCATAATCAGCTCGCCG
TGCGGGTCGACAGCCCCTATGAAGACCCGCAAAAGATCTGGCCACTGCACAAGACGCTGATGAAAGGGATCCTCAATCAG
CACGATACCCGTCCGGGCGGCGCCTGGTCGGTTCAGGGGCAGGATGCCAACTCCGGGGGGATCTGGGCGCCGGTGAAGCT
CCATCTGAGCCGCGGCGTCACTATCGACGAGGTGATCCTGCGCCCGGACTGGCAACAGGGGCTGGAGAAGCCCATGCTGC
GTGCCGAAATCCGCTATCGGGCCGTGACGCCCGGCCCCGTCACGCTGCGCCTGACGGCCACTCCGGACAATTTTTCCGGC
CCGCCTTTTCAGCAGGCCTTTGCGGTCACCCTGGAGAATACCGCAGGCACTCTGCGCGTCTCGCTGCCCATGCCCTCGGC
AAAGCTCTGGTGGCCGGTGGGCACAGGTAAACCGAACCTGTACCGCGTGCGCGCCACCCTCGTGGATAAGCGGGGGATCA
TGGATACCGCCGTCACCCGCACCGGATTACGTAAGGTGGAAGCGCTGGCGGATAATCAGGGCTGGCGCATCAATGACAGA
CGGCTGTTCATCAAGGGCAGCAACTATATTGGCTCGCCGTGGCTGAGCACCATGACGCGGAAAAAGTATCGTCGGGATTT
CAGACTGGTCACGGCGATGAACGCCAACGCCATTCGTGTACACGGCCACGTGGCGGGCCGCGCGCTGTATGAGGTGGCCG
ATGAGATGGGACTGATGATCTGGCAGGATGTCCCGCTGCAGTGGGGTTATGATGACAGCGCCGCCTTCGCCGATAACGCC
GTGCGCCAGACCCGGGCGATGATGGACCAGTTCGGCAATTCACCGGCGATTATTGTCTGGGGCGGTCACAACGAGCCGCC
GTGGAACTCGCCGTGGATGGAGAAACGCTTCCCCGACTGGCGTAAAACCTTAAACCAGACCCTGACGCAGCGGGTGGGCG
ATGCGCTGGCGGAAGACACCTCCCGCATCGTGCATCGCTTTTCCGCTGTCGAAGAGCACTACTGGGCAGGGTGGTATTTC
GGCACCCTGCGCGACCTGCTCGCCCCGGCGAAAACCGGGATTATTACCGAGTTTGGCGCCCAGGCGCTGCCCCGGCTCTC
CACCCTGAAGACCATTATCCCGGCCCGTCTGCTGTGGCCGAAAACCACCGCTGCCGACGACCCCGGCTGGGTGCGATGGA
AGTACCATAATTTTCAGCCTTTCCAGACCTTTAAATTTGCCGGTATTCCACGAGGCAACAATATTCAGGAGATGATTGAA
AACACCCAAGCATATCAGGCCCGGCTGGTGGCGCTGGCGGCGGAGAGCTATCGCCGCCAGCGTTATCAGCCGGTGACCGC
CCTGTTCCACTTTATGTTTGTCGAGACATGGCCCTCCATCAACTGGGGGGTGGTGGATTATCTGCGCCAGCCGAAGGCCG
GTTACTATGCCCTGCAGCGCGCCTATCAGCCGATCCTGCCGTCCATCGAGCCGGTGACCGCCAGCTGGCGTCAGGGAAGC
CCGGCGACGGTGCGCCTGTGGGCGATTAACGATACCTGGGCAGCCTGCGAGGATTGCCGCCTGACCTGGCAGGTCAGGCA
AAATGGCCAGATGCTCGCTCAGGGAGAAACCTCGCTGACCCTGCCGCCCGACGCTGGCCAAATGGTCAGGGAGTTGACCG
TCACCCCCGCCGGCCCGCAGCCCGTGACGATCGTCTACCGCATTGACAACCGTCGCGGCAAACACGTGGGTGCTAACCAG
CATACCGAGCCCGTCGCCGCGGCGCAGGCGGGGAGATGA

Upstream 100 bases:

>100_bases
TCGCCAGACCAAAGCGTGCCGGGCGACCTGCGCGACGCGCTGACCGAGGCGATCGCGGGCTGCCACCTGTGGCTGCGCCG
CAGAACGCAGGAGGTTCCCC

Downstream 100 bases:

>100_bases
AGATGGAGCCAATCGGTGCCGCGGTCAGTCGTGGATTTTCACCCCCAGACATCCGCGAACGAACTCAGGGTTGAAGTGTT
TTACCGCTTCGCCAACATAG

Product: hypothetical protein

Products: NA

Alternate protein names: Glycoside Hydrolase Family Protein; Beta-Galactosidase; Glycoside Hydrolase; Beta-Mannosidase Protein; Glycoside Hydrolase Family 2 Protein; Hydrolase; Mannosidase; Beta-Glucuronidase; Glycosyl Hydrolase; Alpha-L-Arabinofuranosidase B Family Protein; Coagulation Factor 5/8 Type Domain Protein; Glycoside Hydrolase Family 2 TIM Barrel; Exported Glycosyl Hydrolase; Beta-Galactosidase/Beta- Glucuronidase Family Protein; Glycoside Hydrolase Family 2 Gh2E; Family 2 Glycosyl Hydrolase; Beta-Mannosidase Man2A; DS Domain/Family 2 Glycosyl Hydrolase; Beta-Galactosidase/Beta-Glucuronidase-Like Protein; Beta-Glycosidase; Beta-Galactosidase/Beta; Mannosylglycoprotein Endo-Beta-Mannosidase; Beta-Mannosidase-Related Protein; BETA-Mannosidase Protein; Beta-D-Glucuronidase; Beta-Galactosidase/Beta-Glucuronidase

Number of amino acids: Translated: 732; Mature: 732

Protein sequence:

>732_residues
MIARRLLLLVICLPLAHAGSAPVTWSLAGQWRVHDANDSAFAGAQASDGDWRTLRVPANWYSAGYDHQGALWYRHQFTLH
GLTPDAMATLVFDGVDYFAEVALNGKTLTRHEGYFQRFPVDITGAVRRHNQLAVRVDSPYEDPQKIWPLHKTLMKGILNQ
HDTRPGGAWSVQGQDANSGGIWAPVKLHLSRGVTIDEVILRPDWQQGLEKPMLRAEIRYRAVTPGPVTLRLTATPDNFSG
PPFQQAFAVTLENTAGTLRVSLPMPSAKLWWPVGTGKPNLYRVRATLVDKRGIMDTAVTRTGLRKVEALADNQGWRINDR
RLFIKGSNYIGSPWLSTMTRKKYRRDFRLVTAMNANAIRVHGHVAGRALYEVADEMGLMIWQDVPLQWGYDDSAAFADNA
VRQTRAMMDQFGNSPAIIVWGGHNEPPWNSPWMEKRFPDWRKTLNQTLTQRVGDALAEDTSRIVHRFSAVEEHYWAGWYF
GTLRDLLAPAKTGIITEFGAQALPRLSTLKTIIPARLLWPKTTAADDPGWVRWKYHNFQPFQTFKFAGIPRGNNIQEMIE
NTQAYQARLVALAAESYRRQRYQPVTALFHFMFVETWPSINWGVVDYLRQPKAGYYALQRAYQPILPSIEPVTASWRQGS
PATVRLWAINDTWAACEDCRLTWQVRQNGQMLAQGETSLTLPPDAGQMVRELTVTPAGPQPVTIVYRIDNRRGKHVGANQ
HTEPVAAAQAGR

Sequences:

>Translated_732_residues
MIARRLLLLVICLPLAHAGSAPVTWSLAGQWRVHDANDSAFAGAQASDGDWRTLRVPANWYSAGYDHQGALWYRHQFTLH
GLTPDAMATLVFDGVDYFAEVALNGKTLTRHEGYFQRFPVDITGAVRRHNQLAVRVDSPYEDPQKIWPLHKTLMKGILNQ
HDTRPGGAWSVQGQDANSGGIWAPVKLHLSRGVTIDEVILRPDWQQGLEKPMLRAEIRYRAVTPGPVTLRLTATPDNFSG
PPFQQAFAVTLENTAGTLRVSLPMPSAKLWWPVGTGKPNLYRVRATLVDKRGIMDTAVTRTGLRKVEALADNQGWRINDR
RLFIKGSNYIGSPWLSTMTRKKYRRDFRLVTAMNANAIRVHGHVAGRALYEVADEMGLMIWQDVPLQWGYDDSAAFADNA
VRQTRAMMDQFGNSPAIIVWGGHNEPPWNSPWMEKRFPDWRKTLNQTLTQRVGDALAEDTSRIVHRFSAVEEHYWAGWYF
GTLRDLLAPAKTGIITEFGAQALPRLSTLKTIIPARLLWPKTTAADDPGWVRWKYHNFQPFQTFKFAGIPRGNNIQEMIE
NTQAYQARLVALAAESYRRQRYQPVTALFHFMFVETWPSINWGVVDYLRQPKAGYYALQRAYQPILPSIEPVTASWRQGS
PATVRLWAINDTWAACEDCRLTWQVRQNGQMLAQGETSLTLPPDAGQMVRELTVTPAGPQPVTIVYRIDNRRGKHVGANQ
HTEPVAAAQAGR
>Mature_732_residues
MIARRLLLLVICLPLAHAGSAPVTWSLAGQWRVHDANDSAFAGAQASDGDWRTLRVPANWYSAGYDHQGALWYRHQFTLH
GLTPDAMATLVFDGVDYFAEVALNGKTLTRHEGYFQRFPVDITGAVRRHNQLAVRVDSPYEDPQKIWPLHKTLMKGILNQ
HDTRPGGAWSVQGQDANSGGIWAPVKLHLSRGVTIDEVILRPDWQQGLEKPMLRAEIRYRAVTPGPVTLRLTATPDNFSG
PPFQQAFAVTLENTAGTLRVSLPMPSAKLWWPVGTGKPNLYRVRATLVDKRGIMDTAVTRTGLRKVEALADNQGWRINDR
RLFIKGSNYIGSPWLSTMTRKKYRRDFRLVTAMNANAIRVHGHVAGRALYEVADEMGLMIWQDVPLQWGYDDSAAFADNA
VRQTRAMMDQFGNSPAIIVWGGHNEPPWNSPWMEKRFPDWRKTLNQTLTQRVGDALAEDTSRIVHRFSAVEEHYWAGWYF
GTLRDLLAPAKTGIITEFGAQALPRLSTLKTIIPARLLWPKTTAADDPGWVRWKYHNFQPFQTFKFAGIPRGNNIQEMIE
NTQAYQARLVALAAESYRRQRYQPVTALFHFMFVETWPSINWGVVDYLRQPKAGYYALQRAYQPILPSIEPVTASWRQGS
PATVRLWAINDTWAACEDCRLTWQVRQNGQMLAQGETSLTLPPDAGQMVRELTVTPAGPQPVTIVYRIDNRRGKHVGANQ
HTEPVAAAQAGR

Specific function: The Wild-Type Enzyme Is An Ineffective Lactase. Two Classes Of Point Mutations Dramatically Improve Activity Of The Enzyme. [C]

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI84798622, Length=703, Percent_Identity=21.9061166429587, Blast_Score=113, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17550784, Length=502, Percent_Identity=21.3147410358566, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.2.1.23

Molecular weight: Translated: 82624; Mature: 82624

Theoretical pI: Translated: 10.09; Mature: 10.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIARRLLLLVICLPLAHAGSAPVTWSLAGQWRVHDANDSAFAGAQASDGDWRTLRVPANW
CHHHHHHHHHHHHHHHCCCCCCEEEEECCEEEEECCCCCCCCCCCCCCCCEEEEEECCHH
YSAGYDHQGALWYRHQFTLHGLTPDAMATLVFDGVDYFAEVALNGKTLTRHEGYFQRFPV
HCCCCCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHCCC
DITGAVRRHNQLAVRVDSPYEDPQKIWPLHKTLMKGILNQHDTRPGGAWSVQGQDANSGG
CHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCC
IWAPVKLHLSRGVTIDEVILRPDWQQGLEKPMLRAEIRYRAVTPGPVTLRLTATPDNFSG
EEEEEEEEECCCCCHHHEEECCCHHHHHHCHHHHHHEEEEEECCCCEEEEEEECCCCCCC
PPFQQAFAVTLENTAGTLRVSLPMPSAKLWWPVGTGKPNLYRVRATLVDKRGIMDTAVTR
CCCCCEEEEEEECCCCEEEEEECCCCCEEEEECCCCCCCEEEEEEEEHHCCCCHHHHHHH
TGLRKVEALADNQGWRINDRRLFIKGSNYIGSPWLSTMTRKKYRRDFRLVTAMNANAIRV
HHHHHHHHHHCCCCCEEECCEEEEECCCCCCCHHHHHHHHHHHHHCEEEEEEECCCEEEE
HGHVAGRALYEVADEMGLMIWQDVPLQWGYDDSAAFADNAVRQTRAMMDQFGNSPAIIVW
ECCHHHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEE
GGHNEPPWNSPWMEKRFPDWRKTLNQTLTQRVGDALAEDTSRIVHRFSAVEEHYWAGWYF
CCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEHH
GTLRDLLAPAKTGIITEFGAQALPRLSTLKTIIPARLLWPKTTAADDPGWVRWKYHNFQP
HHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCC
FQTFKFAGIPRGNNIQEMIENTQAYQARLVALAAESYRRQRYQPVTALFHFMFVETWPSI
CCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCC
NWGVVDYLRQPKAGYYALQRAYQPILPSIEPVTASWRQGSPATVRLWAINDTWAACEDCR
CCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHCCE
LTWQVRQNGQMLAQGETSLTLPPDAGQMVRELTVTPAGPQPVTIVYRIDNRRGKHVGANQ
EEEEECCCCCEEECCCCEEECCCCHHHHHHHEECCCCCCCCEEEEEEECCCCCCCCCCCC
HTEPVAAAQAGR
CCCCHHHHCCCC
>Mature Secondary Structure
MIARRLLLLVICLPLAHAGSAPVTWSLAGQWRVHDANDSAFAGAQASDGDWRTLRVPANW
CHHHHHHHHHHHHHHHCCCCCCEEEEECCEEEEECCCCCCCCCCCCCCCCEEEEEECCHH
YSAGYDHQGALWYRHQFTLHGLTPDAMATLVFDGVDYFAEVALNGKTLTRHEGYFQRFPV
HCCCCCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHCCC
DITGAVRRHNQLAVRVDSPYEDPQKIWPLHKTLMKGILNQHDTRPGGAWSVQGQDANSGG
CHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCC
IWAPVKLHLSRGVTIDEVILRPDWQQGLEKPMLRAEIRYRAVTPGPVTLRLTATPDNFSG
EEEEEEEEECCCCCHHHEEECCCHHHHHHCHHHHHHEEEEEECCCCEEEEEEECCCCCCC
PPFQQAFAVTLENTAGTLRVSLPMPSAKLWWPVGTGKPNLYRVRATLVDKRGIMDTAVTR
CCCCCEEEEEEECCCCEEEEEECCCCCEEEEECCCCCCCEEEEEEEEHHCCCCHHHHHHH
TGLRKVEALADNQGWRINDRRLFIKGSNYIGSPWLSTMTRKKYRRDFRLVTAMNANAIRV
HHHHHHHHHHCCCCCEEECCEEEEECCCCCCCHHHHHHHHHHHHHCEEEEEEECCCEEEE
HGHVAGRALYEVADEMGLMIWQDVPLQWGYDDSAAFADNAVRQTRAMMDQFGNSPAIIVW
ECCHHHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEE
GGHNEPPWNSPWMEKRFPDWRKTLNQTLTQRVGDALAEDTSRIVHRFSAVEEHYWAGWYF
CCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEHH
GTLRDLLAPAKTGIITEFGAQALPRLSTLKTIIPARLLWPKTTAADDPGWVRWKYHNFQP
HHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCC
FQTFKFAGIPRGNNIQEMIENTQAYQARLVALAAESYRRQRYQPVTALFHFMFVETWPSI
CCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCC
NWGVVDYLRQPKAGYYALQRAYQPILPSIEPVTASWRQGSPATVRLWAINDTWAACEDCR
CCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHCCE
LTWQVRQNGQMLAQGETSLTLPPDAGQMVRELTVTPAGPQPVTIVYRIDNRRGKHVGANQ
EEEEECCCCCEEECCCCEEECCCCHHHHHHHEECCCCCCCCEEEEEEECCCCCCCCCCCC
HTEPVAAAQAGR
CCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA