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The map label for this gene is ycjG [H]

Identifier: 238894371

GI number: 238894371

Start: 2263456

End: 2264484

Strand: Direct

Name: ycjG [H]

Synonym: KP1_2353

Alternate gene names: 238894371

Gene position: 2263456-2264484 (Clockwise)

Preceding gene: 238894369

Following gene: 238894373

Centisome position: 43.13

GC content: 64.92

Gene sequence:

>1029_bases
GTGTATGACATCCGCCAGCTACACGGCAAACCCTATTTAACATTTACCAGAGAGGACAAGGGAATGCGTAACGTGCGAGT
GTATGAGGAAGCCTGGCCGCTCCATACCCCTTTTGTGATCGCCCGCGGTAGCCGCAGCGAGGCAAAAGTGGTGGTAGTGG
AACTGGAAGAGGACGGCGTGAAAGGGGTAGGCGAATGCACACCCTATCCCCGCTATGGCGAGAGCATCGCCTCGGTCATG
GCCCAGGTGATGGCGATCGGCGAGCAACTGGAGGCGGGGCTTACCCGCGAACAGCTGCAGCGGCTGCTGCCGGCAGGCGC
CGCCCGCAATGCCATTGACTGCGCGCTATGGGATCTGCAGGCCCGGCGCGAAGGCAAAACCTTGGCCCAGCTGTTGGGCG
TGGCGTTGCCTGACCGGGTGATTACCGCCCAGACGGTGGTCATTGGCACGCCGGATCAGATGGCGGCCAGCGCCGCGGCG
CTGTGGCAGGCAGGGGCCCAGCTGTTGAAGGTGAAGCTCGACGATCGACTGATCAGCGAGCGGCTAATCGCTATCCGCCA
GGCGGCGCCGGAGGCTACGCTGATTGTTGACGCCAATGAGTCCTGGCACAGCGAGGGGCTGGCGGCGCGCTGTCAGCTGC
TGGCGGACCTCGGTGTGGCGATGCTTGAGCAGCCGCTCCCCGCCGACGACGACTCGGCGCTGGAAAACTTCGTTCATCCG
CTGCCGATTTGCGCCGACGAGAGCTGCCACACCCGGGAGAGCCTGCCGCGGCTGCGCGGGCGCTATGAGATGGTCAATAT
CAAACTGGATAAAACGGGCGGCCTGACCGAAGCGCTGGCTCTGGCCGGGGAGGCGGAACGGCAGGGATTTGAACGGATGC
TGGGCTGTATGCTCTGCACCTCGCGCGGGATCGCCGCCGCGCTCCCTCTGGCGCCGCTGGCGCGCTTCGCTGACCTCGAC
GGCCCGACATGGCTGGCGGTGGACGTTGAGCCGGCGCTGCTGTTCAGCACCGGCGTGCTTCATCTTTAA

Upstream 100 bases:

>100_bases
CCTGCGGAATGGTGCCCTGAACAGAAACGGGGTTGCCCTGGAAATGCACGGTTTGTGACATGGTTATCTTCCTGTTTACA
TATAGTTAACGTCGCGAGTA

Downstream 100 bases:

>100_bases
GCCTGCCAGCGCAGCAGATCGGTCATCGCCGGCAGGTAACGCTCCGTTGCCTCGTCGGCGGAGACCGGCGGGAACTCGGC
GGTGATGCAGGGGAGTCCGA

Product: L-Ala-D/L-Glu epimerase

Products: NA

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MYDIRQLHGKPYLTFTREDKGMRNVRVYEEAWPLHTPFVIARGSRSEAKVVVVELEEDGVKGVGECTPYPRYGESIASVM
AQVMAIGEQLEAGLTREQLQRLLPAGAARNAIDCALWDLQARREGKTLAQLLGVALPDRVITAQTVVIGTPDQMAASAAA
LWQAGAQLLKVKLDDRLISERLIAIRQAAPEATLIVDANESWHSEGLAARCQLLADLGVAMLEQPLPADDDSALENFVHP
LPICADESCHTRESLPRLRGRYEMVNIKLDKTGGLTEALALAGEAERQGFERMLGCMLCTSRGIAAALPLAPLARFADLD
GPTWLAVDVEPALLFSTGVLHL

Sequences:

>Translated_342_residues
MYDIRQLHGKPYLTFTREDKGMRNVRVYEEAWPLHTPFVIARGSRSEAKVVVVELEEDGVKGVGECTPYPRYGESIASVM
AQVMAIGEQLEAGLTREQLQRLLPAGAARNAIDCALWDLQARREGKTLAQLLGVALPDRVITAQTVVIGTPDQMAASAAA
LWQAGAQLLKVKLDDRLISERLIAIRQAAPEATLIVDANESWHSEGLAARCQLLADLGVAMLEQPLPADDDSALENFVHP
LPICADESCHTRESLPRLRGRYEMVNIKLDKTGGLTEALALAGEAERQGFERMLGCMLCTSRGIAAALPLAPLARFADLD
GPTWLAVDVEPALLFSTGVLHL
>Mature_342_residues
MYDIRQLHGKPYLTFTREDKGMRNVRVYEEAWPLHTPFVIARGSRSEAKVVVVELEEDGVKGVGECTPYPRYGESIASVM
AQVMAIGEQLEAGLTREQLQRLLPAGAARNAIDCALWDLQARREGKTLAQLLGVALPDRVITAQTVVIGTPDQMAASAAA
LWQAGAQLLKVKLDDRLISERLIAIRQAAPEATLIVDANESWHSEGLAARCQLLADLGVAMLEQPLPADDDSALENFVHP
LPICADESCHTRESLPRLRGRYEMVNIKLDKTGGLTEALALAGEAERQGFERMLGCMLCTSRGIAAALPLAPLARFADLD
GPTWLAVDVEPALLFSTGVLHL

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has a role in the recycling of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of all the L-Ala-X dipeptides, excep

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=321, Percent_Identity=80.0623052959502, Blast_Score=518, Evalue=1e-148,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 37208; Mature: 37208

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDIRQLHGKPYLTFTREDKGMRNVRVYEEAWPLHTPFVIARGSRSEAKVVVVELEEDGV
CCCCHHHCCCCEEEEECCCCCCCCEEEEHHCCCCCCCEEEEECCCCCCEEEEEEECCCCC
KGVGECTPYPRYGESIASVMAQVMAIGEQLEAGLTREQLQRLLPAGAARNAIDCALWDLQ
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHH
ARREGKTLAQLLGVALPDRVITAQTVVIGTPDQMAASAAALWQAGAQLLKVKLDDRLISE
HHHCHHHHHHHHHHCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHEEHHHHHHHHHH
RLIAIRQAAPEATLIVDANESWHSEGLAARCQLLADLGVAMLEQPLPADDDSALENFVHP
HHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCC
LPICADESCHTRESLPRLRGRYEMVNIKLDKTGGLTEALALAGEAERQGFERMLGCMLCT
CCCCCCCCCHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHCHHHHHHHHHHHHHHHHH
SRGIAAALPLAPLARFADLDGPTWLAVDVEPALLFSTGVLHL
CCCCHHHCCHHHHHHHHCCCCCEEEEEECCHHHHHHCCCCCC
>Mature Secondary Structure
MYDIRQLHGKPYLTFTREDKGMRNVRVYEEAWPLHTPFVIARGSRSEAKVVVVELEEDGV
CCCCHHHCCCCEEEEECCCCCCCCEEEEHHCCCCCCCEEEEECCCCCCEEEEEEECCCCC
KGVGECTPYPRYGESIASVMAQVMAIGEQLEAGLTREQLQRLLPAGAARNAIDCALWDLQ
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHH
ARREGKTLAQLLGVALPDRVITAQTVVIGTPDQMAASAAALWQAGAQLLKVKLDDRLISE
HHHCHHHHHHHHHHCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHEEHHHHHHHHHH
RLIAIRQAAPEATLIVDANESWHSEGLAARCQLLADLGVAMLEQPLPADDDSALENFVHP
HHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCC
LPICADESCHTRESLPRLRGRYEMVNIKLDKTGGLTEALALAGEAERQGFERMLGCMLCT
CCCCCCCCCHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHCHHHHHHHHHHHHHHHHH
SRGIAAALPLAPLARFADLDGPTWLAVDVEPALLFSTGVLHL
CCCCHHHCCHHHHHHHHCCCCCEEEEEECCHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7499381; 9097039; 9278503 [H]