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The map label for this gene is gcvA [H]

Identifier: 238894323

GI number: 238894323

Start: 2214095

End: 2214991

Strand: Direct

Name: gcvA [H]

Synonym: KP1_2302

Alternate gene names: 238894323

Gene position: 2214095-2214991 (Clockwise)

Preceding gene: 238894321

Following gene: 238894324

Centisome position: 42.19

GC content: 63.77

Gene sequence:

>897_bases
ATGAAACTCCCGCCGCTGAATGCGTTGCGCTGCTTTGAGGCGGCGGCGCGGCTGCTCAGCCTGAAGCTGGCCGCCAGTGA
GCTTTGCGTCACGCCCAGCGCCGTCAGCCAGCAGATTGCCCGCCTTGAGGAGACGCTGAATGTCCCGCTATTTATCCGTA
CTCCGCGACGGTTACAGTTAACGGCGGTGGGAGAGATCTACCTGCGGGCCGTTCAGCCGGCGTTTCATCAGATAGCCGCC
GCCACCCAGCGCCTGCAGGTGCCCCCGGGACCAGAAAAGGTAGCTATCAGCTGTACCAGCGGCTTCGCCATTCAGTGGCT
GCTGCCGCGGCTGGCTGATTTTGAACGACGCTATCCGCAGATAGAGATCCAGATCGGCACCACCAACCGGCGAGTGGATT
TGCTGAGTGAAGGGATCGATTTTGCCGTGCGTCATGGTGTCGGCGGCTGGCCGGAACTGCAGGCTTACCGCCTGCTGGAT
GACGATCTGATGCCGGTCTGTAGCCCGCAACTGATTGCTCCGCGCCGGACGCTCACCGATGCGAGCGATCTACTGCAGTA
TCCGCTGCTGCACGATGAACATCGTGAAGACTGGGCGCTGTGGTGTGAGGCCGTGGGGCTGGCGCCGCACTTCGCGCGGC
GTGGGCCGGTGTTCACTGATTCCAACGGCGTGACGGAGGCGGCGTTCGCCGGTATGGGGATGGCATTGCTGCGACGCTCA
TTCATAGCGCCTGCGCTGACGCAGGGGCGGCTGGTTAATCCTCTGGCGCAGCCCATCGCCTGTCCGCTGGCCTATCATCT
GGTTTACCACGAGACCGCGCTGCTGGCGCCTGCCAACCGCTGTTTTCGCGACTGGCTGCTTGGCCAGCGCCCGGCCGTTG
CTGAGGAGCGCGCGTAG

Upstream 100 bases:

>100_bases
TGTAGTCACGAAGGAAGTCAACTGTAGAACCCACGTGCAGCGTGCGACAAACGCGTTATGCTTACATGATTAAGTTTTTC
TTAACTGAGGTTCCATACCC

Downstream 100 bases:

>100_bases
TGAGGGGGGCGCTGTCCGATAAAGATCTGTATGCGTGAATGGGCACAGGAGAATGAGGAAAATCTGTTCCATACTAAAGA
CGGGGCGCGGCGAACAGAAG

Product: putative LysR family regulatory protein

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MKLPPLNALRCFEAAARLLSLKLAASELCVTPSAVSQQIARLEETLNVPLFIRTPRRLQLTAVGEIYLRAVQPAFHQIAA
ATQRLQVPPGPEKVAISCTSGFAIQWLLPRLADFERRYPQIEIQIGTTNRRVDLLSEGIDFAVRHGVGGWPELQAYRLLD
DDLMPVCSPQLIAPRRTLTDASDLLQYPLLHDEHREDWALWCEAVGLAPHFARRGPVFTDSNGVTEAAFAGMGMALLRRS
FIAPALTQGRLVNPLAQPIACPLAYHLVYHETALLAPANRCFRDWLLGQRPAVAEERA

Sequences:

>Translated_298_residues
MKLPPLNALRCFEAAARLLSLKLAASELCVTPSAVSQQIARLEETLNVPLFIRTPRRLQLTAVGEIYLRAVQPAFHQIAA
ATQRLQVPPGPEKVAISCTSGFAIQWLLPRLADFERRYPQIEIQIGTTNRRVDLLSEGIDFAVRHGVGGWPELQAYRLLD
DDLMPVCSPQLIAPRRTLTDASDLLQYPLLHDEHREDWALWCEAVGLAPHFARRGPVFTDSNGVTEAAFAGMGMALLRRS
FIAPALTQGRLVNPLAQPIACPLAYHLVYHETALLAPANRCFRDWLLGQRPAVAEERA
>Mature_298_residues
MKLPPLNALRCFEAAARLLSLKLAASELCVTPSAVSQQIARLEETLNVPLFIRTPRRLQLTAVGEIYLRAVQPAFHQIAA
ATQRLQVPPGPEKVAISCTSGFAIQWLLPRLADFERRYPQIEIQIGTTNRRVDLLSEGIDFAVRHGVGGWPELQAYRLLD
DDLMPVCSPQLIAPRRTLTDASDLLQYPLLHDEHREDWALWCEAVGLAPHFARRGPVFTDSNGVTEAAFAGMGMALLRRS
FIAPALTQGRLVNPLAQPIACPLAYHLVYHETALLAPANRCFRDWLLGQRPAVAEERA

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=295, Percent_Identity=38.6440677966102, Blast_Score=210, Evalue=9e-56,
Organism=Escherichia coli, GI1788706, Length=277, Percent_Identity=31.4079422382672, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI1786448, Length=290, Percent_Identity=32.7586206896552, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI87081978, Length=261, Percent_Identity=31.8007662835249, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786401, Length=275, Percent_Identity=26.5454545454545, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI145693193, Length=294, Percent_Identity=28.2312925170068, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI157672245, Length=259, Percent_Identity=28.957528957529, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1787128, Length=137, Percent_Identity=32.8467153284672, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787589, Length=286, Percent_Identity=26.9230769230769, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787806, Length=252, Percent_Identity=28.968253968254, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790262, Length=123, Percent_Identity=34.9593495934959, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1789440, Length=246, Percent_Identity=25.609756097561, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787879, Length=297, Percent_Identity=28.2828282828283, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33092; Mature: 33092

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLPPLNALRCFEAAARLLSLKLAASELCVTPSAVSQQIARLEETLNVPLFIRTPRRLQL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCEEEE
TAVGEIYLRAVQPAFHQIAAATQRLQVPPGPEKVAISCTSGFAIQWLLPRLADFERRYPQ
EEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCE
IEIQIGTTNRRVDLLSEGIDFAVRHGVGGWPELQAYRLLDDDLMPVCSPQLIAPRRTLTD
EEEEECCCCCHHHHHHHCHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHCC
ASDLLQYPLLHDEHREDWALWCEAVGLAPHFARRGPVFTDSNGVTEAAFAGMGMALLRRS
HHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHH
FIAPALTQGRLVNPLAQPIACPLAYHLVYHETALLAPANRCFRDWLLGQRPAVAEERA
HHHHHHHCCCHHCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKLPPLNALRCFEAAARLLSLKLAASELCVTPSAVSQQIARLEETLNVPLFIRTPRRLQL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCEEEE
TAVGEIYLRAVQPAFHQIAAATQRLQVPPGPEKVAISCTSGFAIQWLLPRLADFERRYPQ
EEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCE
IEIQIGTTNRRVDLLSEGIDFAVRHGVGGWPELQAYRLLDDDLMPVCSPQLIAPRRTLTD
EEEEECCCCCHHHHHHHCHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHCC
ASDLLQYPLLHDEHREDWALWCEAVGLAPHFARRGPVFTDSNGVTEAAFAGMGMALLRRS
HHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHH
FIAPALTQGRLVNPLAQPIACPLAYHLVYHETALLAPANRCFRDWLLGQRPAVAEERA
HHHHHHHCCCHHCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]