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The map label for this gene is trpA

Identifier: 238894315

GI number: 238894315

Start: 2204733

End: 2205542

Strand: Reverse

Name: trpA

Synonym: KP1_2294

Alternate gene names: 238894315

Gene position: 2205542-2204733 (Counterclockwise)

Preceding gene: 238894316

Following gene: 238894311

Centisome position: 42.02

GC content: 64.57

Gene sequence:

>810_bases
ATGGAACGCTATGAGACGCTGTTTGCCCAACTGAAAAATCGCCAGGAAGGCGCCTTCGTCCCCTTTGTCACCCTTGGCGA
TCCGGGACCGGAGCAGTCGCTGAAAATTATCGATGCGCTGATCGAAGGCGGCGCCGATGCCCTTGAACTGGGGATCCCTT
TCTCCGACCCGCTGGCGGACGGCCCGACCATCCAGGGCGCGGCCCTGCGCGCCTTCGCCGCGGGAGTGACCCCAGCGCAG
TGCTTTGAGATGCTGGCGGCGATCCGCCAGAAGCATCCGACCATCCCAATCGGCCTGCTGATGTATGCCAACCTCGTCTT
CAGCCCGGGCATCGATGCGTTCTATGCCCAGTGCGCCCGCGTCGGCGTCGACTCGGTGCTGGTGGCCGACGTGCCGGTGG
AAGAGTCCGCCCCGTTCCGCCAGGCGGCGATGCGCCATAACATCGCGCCGATTTTCATCTGCCCGCCCAATGCGGATGAC
GATTTACTGCGCCAGATTGCCTCTTATGGCCGCGGCTACACCTATCTGCTGTCGCGCGCCGGAGTGACGGGTGCGGAAAA
TCGCGCCGCGCTGCCGCTGCACCACCTGGTGGAGAAGCTGGCGGAATATCACGCCGCGCCGCCGCTGCAGGGCTTTGGTA
TCTCCGCGCCGGAGCAGGTGAGCGCCGCCATTGACGCCGGAGCCGCCGGGGCTATCTCCGGTTCCGCCATCGTCAAAATC
ATCGAACGCCACCTCGATGAGCCGCAGACCATGCTCGACGAGCTGAAAGCCTTCGTCCAGAGCCTGAAGGCAGCGACCAA
AACCGCCTGA

Upstream 100 bases:

>100_bases
ACCCGGAAAAAGAGCAGCTGCTGGTGGTTAATCTCTCCGGCCGCGGCGACAAAGACATTTTCACCGTACATGACATCCTG
AAAGCGCGAGGGGAAATCTG

Downstream 100 bases:

>100_bases
CCATCAGCCGCCTGGCATCGCGCCAGGCGGCGATTTCCACGCGTTACCTTCCTTTCCATGTCATCTTGCACGCCAACACA
TCAGACGGACCGATGATCGA

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MERYETLFAQLKNRQEGAFVPFVTLGDPGPEQSLKIIDALIEGGADALELGIPFSDPLADGPTIQGAALRAFAAGVTPAQ
CFEMLAAIRQKHPTIPIGLLMYANLVFSPGIDAFYAQCARVGVDSVLVADVPVEESAPFRQAAMRHNIAPIFICPPNADD
DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLHHLVEKLAEYHAAPPLQGFGISAPEQVSAAIDAGAAGAISGSAIVKI
IERHLDEPQTMLDELKAFVQSLKAATKTA

Sequences:

>Translated_269_residues
MERYETLFAQLKNRQEGAFVPFVTLGDPGPEQSLKIIDALIEGGADALELGIPFSDPLADGPTIQGAALRAFAAGVTPAQ
CFEMLAAIRQKHPTIPIGLLMYANLVFSPGIDAFYAQCARVGVDSVLVADVPVEESAPFRQAAMRHNIAPIFICPPNADD
DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLHHLVEKLAEYHAAPPLQGFGISAPEQVSAAIDAGAAGAISGSAIVKI
IERHLDEPQTMLDELKAFVQSLKAATKTA
>Mature_269_residues
MERYETLFAQLKNRQEGAFVPFVTLGDPGPEQSLKIIDALIEGGADALELGIPFSDPLADGPTIQGAALRAFAAGVTPAQ
CFEMLAAIRQKHPTIPIGLLMYANLVFSPGIDAFYAQCARVGVDSVLVADVPVEESAPFRQAAMRHNIAPIFICPPNADD
DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLHHLVEKLAEYHAAPPLQGFGISAPEQVSAAIDAGAAGAISGSAIVKI
IERHLDEPQTMLDELKAFVQSLKAATKTA

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=268, Percent_Identity=87.3134328358209, Blast_Score=457, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6321412, Length=268, Percent_Identity=32.089552238806, Blast_Score=141, Evalue=1e-34,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_KLEAE (P00930)

Other databases:

- EMBL:   V00630
- EMBL:   J01738
- ProteinModelPortal:   P00930
- SMR:   P00930
- BRENDA:   4.2.1.20
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 28544; Mature: 28544

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 49-49 ACT_SITE 60-60

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERYETLFAQLKNRQEGAFVPFVTLGDPGPEQSLKIIDALIEGGADALELGIPFSDPLAD
CCHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCC
GPTIQGAALRAFAAGVTPAQCFEMLAAIRQKHPTIPIGLLMYANLVFSPGIDAFYAQCAR
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHH
VGVDSVLVADVPVEESAPFRQAAMRHNIAPIFICPPNADDDLLRQIASYGRGYTYLLSRA
HCCCCEEEECCCCCCCCCHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCHHHHHHHC
GVTGAENRAALPLHHLVEKLAEYHAAPPLQGFGISAPEQVSAAIDAGAAGAISGSAIVKI
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHH
IERHLDEPQTMLDELKAFVQSLKAATKTA
HHHHCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MERYETLFAQLKNRQEGAFVPFVTLGDPGPEQSLKIIDALIEGGADALELGIPFSDPLAD
CCHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCC
GPTIQGAALRAFAAGVTPAQCFEMLAAIRQKHPTIPIGLLMYANLVFSPGIDAFYAQCAR
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHH
VGVDSVLVADVPVEESAPFRQAAMRHNIAPIFICPPNADDDLLRQIASYGRGYTYLLSRA
HCCCCEEEECCCCCCCCCHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCHHHHHHHC
GVTGAENRAALPLHHLVEKLAEYHAAPPLQGFGISAPEQVSAAIDAGAAGAISGSAIVKI
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHH
IERHLDEPQTMLDELKAFVQSLKAATKTA
HHHHCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6262736; 4571778