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The map label for this gene is pncA [H]

Identifier: 238894257

GI number: 238894257

Start: 2144931

End: 2145371

Strand: Reverse

Name: pncA [H]

Synonym: KP1_2228

Alternate gene names: 238894257

Gene position: 2145371-2144931 (Counterclockwise)

Preceding gene: 238894258

Following gene: 238894256

Centisome position: 40.88

GC content: 62.81

Gene sequence:

>441_bases
ATGGCACATCGAGCGCTGTTACTGGTTGATTTGCAGAACGACTTTTGCGCCGGCGGCGCGCTGGCCGTTCCTGAAGGCGA
CAGTACCGTCGACGTAGCCAATGCGCTTATCGACTGGAGCCTGGCGCGCGGCGAGCCCATCGTCGCCAGCCAGGACTGGC
ATCCGGCGGATCACGGCAGTTTCGCCAGCCAGCACCAGGTGGAACCCTACACCGAGGGCGAGCTGGACGGCCTGGCGCAA
ACTTTCTGGCCGGATCACTGCGTGCAGCACAGCGAAGGCGCGGCGCTGCATCCGCTGCTCAAGCAGCAGGCGATCGCCGC
TGTGTTCCACAAAGGGCAAAACCGGATCATCGACAGCTATAGCGCTTTCTTTGACAACGGTCACCGGCAGAAAACCGAAC
TGGACGGCTGGCTCTGCGTGGTCAGGGGATCGTGGAGCTGA

Upstream 100 bases:

>100_bases
CTATCTGCTTAGCCAGCAGCTGGACGTTGACGCCATTCGCGCGGCGATGCAGCAAAATCTGCGTGGCGAACTCACCCCCG
ACGAGGCTTAAGGAGGACGT

Downstream 100 bases:

>100_bases
CCGTTCTCGGCCTCGCCACCGATTACTGCGTTAAGTTCACCGTGCTGGATGCCCTGGCGCTGGGCTACGCGGTCAACGTC
ATCACCGACGGCTGTCGCGG

Product: nicotinamidase and pyrazinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 146; Mature: 145

Protein sequence:

>146_residues
MAHRALLLVDLQNDFCAGGALAVPEGDSTVDVANALIDWSLARGEPIVASQDWHPADHGSFASQHQVEPYTEGELDGLAQ
TFWPDHCVQHSEGAALHPLLKQQAIAAVFHKGQNRIIDSYSAFFDNGHRQKTELDGWLCVVRGSWS

Sequences:

>Translated_146_residues
MAHRALLLVDLQNDFCAGGALAVPEGDSTVDVANALIDWSLARGEPIVASQDWHPADHGSFASQHQVEPYTEGELDGLAQ
TFWPDHCVQHSEGAALHPLLKQQAIAAVFHKGQNRIIDSYSAFFDNGHRQKTELDGWLCVVRGSWS
>Mature_145_residues
AHRALLLVDLQNDFCAGGALAVPEGDSTVDVANALIDWSLARGEPIVASQDWHPADHGSFASQHQVEPYTEGELDGLAQT
FWPDHCVQHSEGAALHPLLKQQAIAAVFHKGQNRIIDSYSAFFDNGHRQKTELDGWLCVVRGSWS

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=138, Percent_Identity=81.8840579710145, Blast_Score=238, Evalue=9e-65,
Organism=Caenorhabditis elegans, GI193206735, Length=155, Percent_Identity=36.1290322580645, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17543130, Length=155, Percent_Identity=36.1290322580645, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71999339, Length=156, Percent_Identity=32.6923076923077, Blast_Score=68, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6321401, Length=148, Percent_Identity=31.0810810810811, Blast_Score=72, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24648247, Length=156, Percent_Identity=34.6153846153846, Blast_Score=78, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 15985; Mature: 15854

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHRALLLVDLQNDFCAGGALAVPEGDSTVDVANALIDWSLARGEPIVASQDWHPADHGS
CCCCEEEEEECCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCC
FASQHQVEPYTEGELDGLAQTFWPDHCVQHSEGAALHPLLKQQAIAAVFHKGQNRIIDSY
CCCCCCCCCCCCCCHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHH
SAFFDNGHRQKTELDGWLCVVRGSWS
HHHHCCCCCCCCCCCCEEEEEECCCC
>Mature Secondary Structure 
AHRALLLVDLQNDFCAGGALAVPEGDSTVDVANALIDWSLARGEPIVASQDWHPADHGS
CCCEEEEEECCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCC
FASQHQVEPYTEGELDGLAQTFWPDHCVQHSEGAALHPLLKQQAIAAVFHKGQNRIIDSY
CCCCCCCCCCCCCCHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHH
SAFFDNGHRQKTELDGWLCVVRGSWS
HHHHCCCCCCCCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]