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The map label for this gene is pncA [H]

Identifier: 238894256

GI number: 238894256

Start: 2144728

End: 2144940

Strand: Reverse

Name: pncA [H]

Synonym: KP1_2227

Alternate gene names: 238894256

Gene position: 2144940-2144728 (Counterclockwise)

Preceding gene: 238894257

Following gene: 238894255

Centisome position: 40.87

GC content: 64.32

Gene sequence:

>213_bases
GTGGAGCTGACCGTTCTCGGCCTCGCCACCGATTACTGCGTTAAGTTCACCGTGCTGGATGCCCTGGCGCTGGGCTACGC
GGTCAACGTCATCACCGACGGCTGTCGCGGCGTAAATCTGCAGCCGCAGGACAGCAGCCAGGCGTTTATGGAGATGGCCG
CCGCGGGCGCCACGCTCTATACCCTCGACGACTGGCGGGAAACTCAGGCCTGA

Upstream 100 bases:

>100_bases
AAGGGCAAAACCGGATCATCGACAGCTATAGCGCTTTCTTTGACAACGGTCACCGGCAGAAAACCGAACTGGACGGCTGG
CTCTGCGTGGTCAGGGGATC

Downstream 100 bases:

>100_bases
TCCCTTCCCCCCGGCGCCCTCGCGCCGGGTCTTCTTCTGCCGCCTCGCTATTGTCCATAAATTGAACTCTCTCGCAGTTT
CAACAGGTTATTGCTGCTAG

Product: nicotinamidase and pyrazinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 70; Mature: 70

Protein sequence:

>70_residues
MELTVLGLATDYCVKFTVLDALALGYAVNVITDGCRGVNLQPQDSSQAFMEMAAAGATLYTLDDWRETQA

Sequences:

>Translated_70_residues
MELTVLGLATDYCVKFTVLDALALGYAVNVITDGCRGVNLQPQDSSQAFMEMAAAGATLYTLDDWRETQA
>Mature_70_residues
MELTVLGLATDYCVKFTVLDALALGYAVNVITDGCRGVNLQPQDSSQAFMEMAAAGATLYTLDDWRETQA

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=69, Percent_Identity=84.0579710144928, Blast_Score=124, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 7549; Mature: 7549

Theoretical pI: Translated: 3.75; Mature: 3.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELTVLGLATDYCVKFTVLDALALGYAVNVITDGCRGVNLQPQDSSQAFMEMAAAGATLY
CCEEEEECCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCHHHHHHHHHCCCEEE
TLDDWRETQA
EECCCCCCCC
>Mature Secondary Structure
MELTVLGLATDYCVKFTVLDALALGYAVNVITDGCRGVNLQPQDSSQAFMEMAAAGATLY
CCEEEEECCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCHHHHHHHHHCCCEEE
TLDDWRETQA
EECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]