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The map label for this gene is potB [H]

Identifier: 238894169

GI number: 238894169

Start: 2072887

End: 2073744

Strand: Reverse

Name: potB [H]

Synonym: KP1_2129

Alternate gene names: 238894169

Gene position: 2073744-2072887 (Counterclockwise)

Preceding gene: 238894170

Following gene: 238894168

Centisome position: 39.51

GC content: 56.41

Gene sequence:

>858_bases
ATGAAGAACACCAGTAAATTCCAGAATGTGGTGATCGCCACTATCGTCGGTTGGCTGGTGCTGTTTGTCTTCCTGCCCAA
CCTGATGATCATCGCCACCAGCTTTTTGACCCGCGACGATGCCAACTTCGTCAAACTGGTCTTCACGCTGGATAACTATT
CGCGGCTACTGGATCCGCTCTATTATGACGTTCTGCTGCATTCGCTAAACATGGCGCTGCTGGCGACCCTCGCCTGCCTG
GCGCTGGGCTATCCGTTTGCGTGGTTCCTCGCCCGGCTGCCGCAGAAGGTACGGCCCCTGCTGCTGTTCCTGCTGATCGT
CCCTTTTTGGACCAACTCGTTGATCCGCATCTACGGGCTGAAGATTTTCCTCAGCACTAAAGGTTACCTGAACGAGTTTC
TGCTGTGGCTGGGGGTGATCGACACCCCGATACGCATTATGTTCACCCCCTCGGCGGTGATCATCGGCCTGGTGTACATC
CTGCTGCCGTTTATGGTGATGCCGCTCTACTCCAGCATTGAGAAACTGGATCGCCCGCTGCTGGAAGCAGCGAAAGATCT
CGGCGCCAGCAAGCTGCAGACCTTTATCCGCATTATCATCCCGCTGACCATGCCGGGGATCATCGCCGGCTGTCTGCTGG
TGATGCTGCCGGCGATGGGGTTGTTCTACGTTTCCGACCTGATGGGCGGCGCCAAGAACCTGCTGATCGGCAACGTGATC
AAAAGCCAGTTCCTCAATATCCGTGACTGGCCGTTCGGCGCGGCAACCAGCATTACCTTAACCCTGGTGATGGGCCTGAT
GCTGTTGATTTACTGGCGCGCGGCGCGCCTGCTGAATAAAAAGGTGGAGCTGGAATGA

Upstream 100 bases:

>100_bases
GTCAGCGAGTTCTTTAACGAAGACGACCCGGATTTCGACCACTCTCTTGACCAGAAAATGTCTATCAATTGGGTGGAAAG
CTGGGAGGTGGTGCTGGCCG

Downstream 100 bases:

>100_bases
TCGGTCGACTGCTGCGCGGCGGCTTTATGACCGCCATCTACGCGTATCTCTACATCCCTATTATTATTCTGATCGTTAAC
TCGTTTAACCGGTCGCGCTT

Product: spermidine/putrescine ABC transporter membrane protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MKNTSKFQNVVIATIVGWLVLFVFLPNLMIIATSFLTRDDANFVKLVFTLDNYSRLLDPLYYDVLLHSLNMALLATLACL
ALGYPFAWFLARLPQKVRPLLLFLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
LLPFMVMPLYSSIEKLDRPLLEAAKDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPAMGLFYVSDLMGGAKNLLIGNVI
KSQFLNIRDWPFGAATSITLTLVMGLMLLIYWRAARLLNKKVELE

Sequences:

>Translated_285_residues
MKNTSKFQNVVIATIVGWLVLFVFLPNLMIIATSFLTRDDANFVKLVFTLDNYSRLLDPLYYDVLLHSLNMALLATLACL
ALGYPFAWFLARLPQKVRPLLLFLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
LLPFMVMPLYSSIEKLDRPLLEAAKDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPAMGLFYVSDLMGGAKNLLIGNVI
KSQFLNIRDWPFGAATSITLTLVMGLMLLIYWRAARLLNKKVELE
>Mature_285_residues
MKNTSKFQNVVIATIVGWLVLFVFLPNLMIIATSFLTRDDANFVKLVFTLDNYSRLLDPLYYDVLLHSLNMALLATLACL
ALGYPFAWFLARLPQKVRPLLLFLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
LLPFMVMPLYSSIEKLDRPLLEAAKDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPAMGLFYVSDLMGGAKNLLIGNVI
KSQFLNIRDWPFGAATSITLTLVMGLMLLIYWRAARLLNKKVELE

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=275, Percent_Identity=93.4545454545455, Blast_Score=516, Evalue=1e-148,
Organism=Escherichia coli, GI1787080, Length=221, Percent_Identity=46.606334841629, Blast_Score=203, Evalue=1e-53,
Organism=Escherichia coli, GI1787713, Length=266, Percent_Identity=30.8270676691729, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1786980, Length=185, Percent_Identity=31.3513513513514, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1788764, Length=204, Percent_Identity=26.9607843137255, Blast_Score=64, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32169; Mature: 32169

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure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HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]