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The map label for this gene is holB [H]

Identifier: 238894133

GI number: 238894133

Start: 2033712

End: 2034716

Strand: Direct

Name: holB [H]

Synonym: KP1_2088

Alternate gene names: 238894133

Gene position: 2033712-2034716 (Clockwise)

Preceding gene: 238894132

Following gene: 238894134

Centisome position: 38.75

GC content: 63.38

Gene sequence:

>1005_bases
ATGAAATGGTATCCGTGGCTGCGCCCCTCGTTCGAGCAACTGGTCGGTAGCTATCAGGCCGGACGAGGCCACCACGCCTT
ACTGTTACAGTCCCTGAACGGGATGGGCGGTGAGGCGTTGATTTACGCGCTGTGCCGCTTCCTGATGTGCCGCCAGCCCG
AAGGACATAAAAGCTGCGGCCACTGCCATAGCTGTCAGCTGATGCAGGCCGGCACGCACCCCGATTACTATGCGCTCAGC
CCCGAGAAGGGCAAGAGCGCGCTGGGTATTGACGCGGTGCGCGATGTCAATGAAAAGCTCTATGAACATGCGCGCCTTGG
TGGGGCAAAAGTGGTCTGGATTAGCGATGCTGCCTTGCTGACCGATGCCGCCGCCAACGCGTTGCTGAAAACGCTGGAGG
AGCCCCCGGAGAATACCTGGTTCTTCCTCGCCTGCGAGGAGCCGGCGCGTCTGCTGACCACTCTGCGCAGCCGCTGTCGC
CTGCATCATCTGGCGCCGCCGTCTGAGCCTTATGCTCTGGCCTGGCTGGAGCGTGAGGTGAGCCTGCCGCAGGAGTCATT
GTTGACCGCGTTACGCCTGTGCGCCAGCGCGCCGGCTGCGGCGCTCGAACTGTTACAGGAACCGCTGTGGACGGCGCGGC
AGCAGTTGTGTCAGGCGCTGGCGGCTACCCTGGCGAGCGGTGACTGGCTGGCGCTGCTGCCGATACTCAATCATGAACAG
GCGGCGGTTCGCCTGCACTGGCTGGCGTCGCTGCTGGTCGATGCGCAAAAACGACAGCAGGGGATTACCCTGGTGAGTAA
CCCTGACGTCTGGCCGCTGCTGGAGCAGCTGGCGCACAGCCTGCCCGCCGCCCGGCTGCAGGCGATTGCCCATGACGTCT
GTACCTGCCGCGAGCAATTGCTCAACGTTATCGGTGTCAACCGCGAACTGCTGCTTACCGAGCGCCTGCTGCGCTGGGAA
CATTACCTGCAACCCGGGACGGGTTTACCCGTCTCCCACCTCTGA

Upstream 100 bases:

>100_bases
CCGATCCTTCTATCCGCACGGTTGACGCAACTCAGCCGCTGGATGCCGTGGCGCGCGACATTCGCGCCACGATCGCCCAG
TGGATGGCGGAGCAAGCGGC

Downstream 100 bases:

>100_bases
GTGAGACATTATGTTTTTAGTCGACTCCCACTGCCATCTTGATGGTCTGGATTATCAAACTCTACACAAAAACGTCGACG
ACGTGCTGGCAAAAGCGGCC

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MKWYPWLRPSFEQLVGSYQAGRGHHALLLQSLNGMGGEALIYALCRFLMCRQPEGHKSCGHCHSCQLMQAGTHPDYYALS
PEKGKSALGIDAVRDVNEKLYEHARLGGAKVVWISDAALLTDAAANALLKTLEEPPENTWFFLACEEPARLLTTLRSRCR
LHHLAPPSEPYALAWLEREVSLPQESLLTALRLCASAPAAALELLQEPLWTARQQLCQALAATLASGDWLALLPILNHEQ
AAVRLHWLASLLVDAQKRQQGITLVSNPDVWPLLEQLAHSLPAARLQAIAHDVCTCREQLLNVIGVNRELLLTERLLRWE
HYLQPGTGLPVSHL

Sequences:

>Translated_334_residues
MKWYPWLRPSFEQLVGSYQAGRGHHALLLQSLNGMGGEALIYALCRFLMCRQPEGHKSCGHCHSCQLMQAGTHPDYYALS
PEKGKSALGIDAVRDVNEKLYEHARLGGAKVVWISDAALLTDAAANALLKTLEEPPENTWFFLACEEPARLLTTLRSRCR
LHHLAPPSEPYALAWLEREVSLPQESLLTALRLCASAPAAALELLQEPLWTARQQLCQALAATLASGDWLALLPILNHEQ
AAVRLHWLASLLVDAQKRQQGITLVSNPDVWPLLEQLAHSLPAARLQAIAHDVCTCREQLLNVIGVNRELLLTERLLRWE
HYLQPGTGLPVSHL
>Mature_334_residues
MKWYPWLRPSFEQLVGSYQAGRGHHALLLQSLNGMGGEALIYALCRFLMCRQPEGHKSCGHCHSCQLMQAGTHPDYYALS
PEKGKSALGIDAVRDVNEKLYEHARLGGAKVVWISDAALLTDAAANALLKTLEEPPENTWFFLACEEPARLLTTLRSRCR
LHHLAPPSEPYALAWLEREVSLPQESLLTALRLCASAPAAALELLQEPLWTARQQLCQALAATLASGDWLALLPILNHEQ
AAVRLHWLASLLVDAQKRQQGITLVSNPDVWPLLEQLAHSLPAARLQAIAHDVCTCREQLLNVIGVNRELLLTERLLRWE
HYLQPGTGLPVSHL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=334, Percent_Identity=69.7604790419162, Blast_Score=432, Evalue=1e-122,
Organism=Escherichia coli, GI1786676, Length=174, Percent_Identity=32.7586206896552, Blast_Score=84, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008921
- InterPro:   IPR004622
- InterPro:   IPR015199 [H]

Pfam domain/function: PF09115 DNApol3-delta_C [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 37138; Mature: 37138

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKWYPWLRPSFEQLVGSYQAGRGHHALLLQSLNGMGGEALIYALCRFLMCRQPEGHKSCG
CCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCC
HCHSCQLMQAGTHPDYYALSPEKGKSALGIDAVRDVNEKLYEHARLGGAKVVWISDAALL
CCCCCHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCHHHH
TDAAANALLKTLEEPPENTWFFLACEEPARLLTTLRSRCRLHHLAPPSEPYALAWLEREV
HHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
SLPQESLLTALRLCASAPAAALELLQEPLWTARQQLCQALAATLASGDWLALLPILNHEQ
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHCCHH
AAVRLHWLASLLVDAQKRQQGITLVSNPDVWPLLEQLAHSLPAARLQAIAHDVCTCREQL
HHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
LNVIGVNRELLLTERLLRWEHYLQPGTGLPVSHL
HHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKWYPWLRPSFEQLVGSYQAGRGHHALLLQSLNGMGGEALIYALCRFLMCRQPEGHKSCG
CCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCC
HCHSCQLMQAGTHPDYYALSPEKGKSALGIDAVRDVNEKLYEHARLGGAKVVWISDAALL
CCCCCHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCHHHH
TDAAANALLKTLEEPPENTWFFLACEEPARLLTTLRSRCRLHHLAPPSEPYALAWLEREV
HHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
SLPQESLLTALRLCASAPAAALELLQEPLWTARQQLCQALAATLASGDWLALLPILNHEQ
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHCCHH
AAVRLHWLASLLVDAQKRQQGITLVSNPDVWPLLEQLAHSLPAARLQAIAHDVCTCREQL
HHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
LNVIGVNRELLLTERLLRWEHYLQPGTGLPVSHL
HHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8505303; 8509334; 8905232; 9278503; 8505304; 1575709; 9363942 [H]