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The map label for this gene is dsbA [H]

Identifier: 238894088

GI number: 238894088

Start: 1988130

End: 1988798

Strand: Direct

Name: dsbA [H]

Synonym: KP1_2039

Alternate gene names: 238894088

Gene position: 1988130-1988798 (Clockwise)

Preceding gene: 238894087

Following gene: 238894091

Centisome position: 37.88

GC content: 40.66

Gene sequence:

>669_bases
ATGGATACTTGTAAGGGAAGCACAATGGTCGTACATGGGAAAGTTATCAAGTTATTAATTACTATTTTGATGGTGGGGTT
GTCATCCGCTGCTTATAGTAAAGATTATCAGGCAGGGAAAAACTTCACTGTTATTCATTCTACTGTTAAGCAACCGCCGC
CACTGGTTGAGTTTTTTTCCTTCTACTGCGGACCTTGTTATGCGTTTGCAGAGCGCATCAATGTTGATACAGCTATCAGA
AAGCGCCTGCCCGACGATATGAAACTGGAAAAATACCATGTCAGCCAGATGGGGCCTTTAGGACCAGCATTAACAGAAGC
ATGGGCCGTAGCACAATATGCTGGGGTAGATGGCAAGGTGGAAAAGTTATTGTTTGAGGGTTTACAGGTTAAGCGTGATA
TTAAAACGGCAGCTGATATTGTTAAGGTGTTTAATCAACTTGGCATTACGTCAGAGAAATATGCCGAAATGCAAAGTAAC
TTTATGGTAAAAGCTTTAATTGCCAGACAGGATAATCTGGTCGAAAAAATGAAAGTGCATGGCACGCCTTCTTTTTATGT
TTCAGGAAAATATCATATTAACAATGCATCACTCGCGCAAGATGACTATGATACCTATGCAGAGGATATGGCTAATCTGG
TTTTATTTTTGCTTAACAAGCCCCTGTAA

Upstream 100 bases:

>100_bases
TAGAATTTCTTCTCTGTAATACAAGAATATCTGCTTTGCTATACATAAGCGCCTTTCTAATAATTTCTTTCGTCTTACGC
CGTTTTACTCATTTAATTGT

Downstream 100 bases:

>100_bases
CACGAAGTGAGAAAGTATCATTAGGTTTGTCTTTTTCGTTCTGTCCGGGTGGCGTTGCCACTCGTCGAACCCCGGTCGGG
GGTTCTCATCCCCCCCGGCG

Product: putative DsbA oxidoreductase

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MDTCKGSTMVVHGKVIKLLITILMVGLSSAAYSKDYQAGKNFTVIHSTVKQPPPLVEFFSFYCGPCYAFAERINVDTAIR
KRLPDDMKLEKYHVSQMGPLGPALTEAWAVAQYAGVDGKVEKLLFEGLQVKRDIKTAADIVKVFNQLGITSEKYAEMQSN
FMVKALIARQDNLVEKMKVHGTPSFYVSGKYHINNASLAQDDYDTYAEDMANLVLFLLNKPL

Sequences:

>Translated_222_residues
MDTCKGSTMVVHGKVIKLLITILMVGLSSAAYSKDYQAGKNFTVIHSTVKQPPPLVEFFSFYCGPCYAFAERINVDTAIR
KRLPDDMKLEKYHVSQMGPLGPALTEAWAVAQYAGVDGKVEKLLFEGLQVKRDIKTAADIVKVFNQLGITSEKYAEMQSN
FMVKALIARQDNLVEKMKVHGTPSFYVSGKYHINNASLAQDDYDTYAEDMANLVLFLLNKPL
>Mature_222_residues
MDTCKGSTMVVHGKVIKLLITILMVGLSSAAYSKDYQAGKNFTVIHSTVKQPPPLVEFFSFYCGPCYAFAERINVDTAIR
KRLPDDMKLEKYHVSQMGPLGPALTEAWAVAQYAGVDGKVEKLLFEGLQVKRDIKTAADIVKVFNQLGITSEKYAEMQSN
FMVKALIARQDNLVEKMKVHGTPSFYVSGKYHINNASLAQDDYDTYAEDMANLVLFLLNKPL

Specific function: Required for disulfide bond formation in some periplasmic proteins such as PhoA or OmpA. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbA is reoxidized by DsbB. It is required for pilus biogenesis [H]

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Escherichia coli, GI1790291, Length=205, Percent_Identity=36.5853658536585, Blast_Score=151, Evalue=3e-38,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001853
- InterPro:   IPR023205
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01323 DSBA [H]

EC number: NA

Molecular weight: Translated: 24757; Mature: 24757

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: PS00194 THIOREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTCKGSTMVVHGKVIKLLITILMVGLSSAAYSKDYQAGKNFTVIHSTVKQPPPLVEFFS
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCEEEEEHHHCCCCCHHHHHH
FYCGPCYAFAERINVDTAIRKRLPDDMKLEKYHVSQMGPLGPALTEAWAVAQYAGVDGKV
HHHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHH
EKLLFEGLQVKRDIKTAADIVKVFNQLGITSEKYAEMQSNFMVKALIARQDNLVEKMKVH
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GTPSFYVSGKYHINNASLAQDDYDTYAEDMANLVLFLLNKPL
CCCCEEEECEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDTCKGSTMVVHGKVIKLLITILMVGLSSAAYSKDYQAGKNFTVIHSTVKQPPPLVEFFS
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCEEEEEHHHCCCCCHHHHHH
FYCGPCYAFAERINVDTAIRKRLPDDMKLEKYHVSQMGPLGPALTEAWAVAQYAGVDGKV
HHHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHH
EKLLFEGLQVKRDIKTAADIVKVFNQLGITSEKYAEMQSNFMVKALIARQDNLVEKMKVH
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GTPSFYVSGKYHINNASLAQDDYDTYAEDMANLVLFLLNKPL
CCCCEEEECEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10438793; 11586360; 12142430 [H]