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The map label for this gene is rlmL [H]

Identifier: 238894004

GI number: 238894004

Start: 1900358

End: 1902463

Strand: Direct

Name: rlmL [H]

Synonym: KP1_1949

Alternate gene names: 238894004

Gene position: 1900358-1902463 (Clockwise)

Preceding gene: 238894002

Following gene: 238894005

Centisome position: 36.21

GC content: 59.69

Gene sequence:

>2106_bases
ATGAATTCTCTGTTTGCCAGTACGGCTCGTGGGCTGGAAGAGCTGTTAAAAACTGAACTGGAAGGGCTTGGCGCGACGGA
CTGCCAGGTAGTCCAGGGCGGGGTCCATTTTCAGGGGGATACGCGCCTGCTGTATCAAAGCCTGATGTGGAGCCGGCTGG
CTTCGCGCATCATGCTGCCGCTGGGCGAATGTCGCGTATACAGCGATCTGGATCTGTACCTCGGCGTACAGGCCATTCCG
TGGACGGAGATGTTCAACCCTGGCGCCACCTTCGCGGTGCATTTTAGCGGCCTCAATGACGAGATCCGTAACAGCCAGTA
CGGCGCGCTGAAGGTTAAAGACGCCATTGTCGACAGCTTCACCCGTAAGAATCTGCCGCGCCCGAACGTCGATCGTGAGT
CGCCCGATCTGCGGATCAACGTCTGGCTGAACAAAGAGACGGCGCACATCTCGCTGGATCTGAGCGGCGAAGGCCTACAC
CTGCGCGGCTATCGCGATGGTACCGGAATGGCGCCGATCAAAGAAAATCTCGCGGCGGCGATCGTCATGCGCTCTGGCTG
GGTACCGGGTACGCCGCTGCTCGATCCGATGTGCGGCTCCGGTACCCTGCTGATTGAAGCGGCGATGCTGGCGACCGACC
GCGCGCCGGGCCTGCACCGCGGCCACTGGGGCTTTGGCGGCTGGGCGCAGCACGATGACGCCATCTGGAAAGAGGTGAAG
GCGGAAGCGCAAACCCGCGCTCGTCAGGGATTGGCAGCGTATGAGTCACGCTTCTACGGCTCAGACGTCGACGTGCGCGT
AATCGAGCGCGCGCGCCGCAACGCCCGCCGCGCCGGCATTGGCGAGCTGGTCGATTTTGACGTGAAAGACGTCGCCCAAC
TGAACAACCCGCTGCCGAAAGGCCCGTACGGGACGGTGATCAGTAACCCGCCGTACGGCGAACGTCTGGAAAGCGAGCCA
GCGCTGATCGCTCTGCACAGCCTGCTTGGCCGGATAATGAAAAGTCAGTTCGGCGGCTGGAACCTGTCGGTATTCAGCGC
TTCGCCGGAGCTGCTGAGTTGCCTGCAGCTGCGTGCCGACAAGCAGTTTAAAGCCAAGAACGGCCCGCTGGACTGCGTGC
AGAAGAATTATCACCTGGCGGAGAGCGAAGGCGGCAAGCCGGCGATGCTGGCGGAGGATTTTGCTAACCGTCTGCGTAAG
AACCTGAAGAAGTTTGAAAAATGGGCCCGCCAGGAAGGAATCGAATGCTATCGCCTGTACGATGCTGATCTACCGGAATA
CAACGTGGCCATTGACCGCTATGCCGACTGGGTGGTGGTCCAGGAGTATGCCCCGCCGAAAACCGTCGACGCGCATAAGG
CGCGCCAGCGTCTGTTCGACATCATCGCTGCGACTATCGCCGTACTGGACATGGCGCCTAACAAGCTGGTGCTGAAAACC
CGTGAACGGCAGAAGGGGAAAAACCAGTATCAGAAGATGGCGGAGAAGGGCGACTTTATTGAGGTACAGGAGTATAACGC
GCGCCTGTGGGTCAACCTCACCGACTACCTGGATACCGGTCTGTTCCTCGACCACCGTATCGCCCGTCGCATGCTCGGCC
AGATGAGTAAAGGTAAGGACTTCCTGAACCTGTTCTCCTATACCGGCAGCGCCAGCGTCCACGCAGGCCTCGGCGGTGCG
CGATCCACCACCACGGTGGATATGTCACGCACCTACCTGGAGTGGGCGGAACGCAACCTGCGTCTCAATGGCCTGACCGG
CCGCGCGCATCGCCTGATGCAGGCGGACGTGCTGGGTTGGCTGCGGGAGAGCACCGAGCAGTTCGATCTGATCTTTATCG
ATCCGCCGACCTTTTCCAACTCCAAACGAATGGAAGATGCGTTTGACGTCCAGCGCGATCACATTCGCCTGATGACCGAT
CTAAAACGTCTGCTGCGAAAAGGCGGCACCATTATGTTCTCGAATAACAAACGCGGTTTCCGTATGGATCATGACGGGCT
GGCTGCGCTTGGCCTGAAGGCGCAAGAGATTTCGCAAAAAACGCTGTCGCAGGATTTTGCCCGCAACCGTCAGATCCACA
ACTGCTGGCTGATTACCGCAGCCTGA

Upstream 100 bases:

>100_bases
TACGCTGCCTCCGGGTTCCAAAAATAAGCCTATAACTTTATGACATAGGGCACATATTGGCTATAATGCGCACCAATTTT
CTTTAAGTAAAAGTGACGAT

Downstream 100 bases:

>100_bases
AAGGAATAAATAATGTCACTGATTAGTATGCACGGCGCCTGGCTTTCCTTCAGCGATGCGCCGCTGCTGGATAATGCCGA
GCTGCATATTGAAGATAACG

Product: 23S rRNA m(2)G2445 methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 701; Mature: 701

Protein sequence:

>701_residues
MNSLFASTARGLEELLKTELEGLGATDCQVVQGGVHFQGDTRLLYQSLMWSRLASRIMLPLGECRVYSDLDLYLGVQAIP
WTEMFNPGATFAVHFSGLNDEIRNSQYGALKVKDAIVDSFTRKNLPRPNVDRESPDLRINVWLNKETAHISLDLSGEGLH
LRGYRDGTGMAPIKENLAAAIVMRSGWVPGTPLLDPMCGSGTLLIEAAMLATDRAPGLHRGHWGFGGWAQHDDAIWKEVK
AEAQTRARQGLAAYESRFYGSDVDVRVIERARRNARRAGIGELVDFDVKDVAQLNNPLPKGPYGTVISNPPYGERLESEP
ALIALHSLLGRIMKSQFGGWNLSVFSASPELLSCLQLRADKQFKAKNGPLDCVQKNYHLAESEGGKPAMLAEDFANRLRK
NLKKFEKWARQEGIECYRLYDADLPEYNVAIDRYADWVVVQEYAPPKTVDAHKARQRLFDIIAATIAVLDMAPNKLVLKT
RERQKGKNQYQKMAEKGDFIEVQEYNARLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSASVHAGLGGA
RSTTTVDMSRTYLEWAERNLRLNGLTGRAHRLMQADVLGWLRESTEQFDLIFIDPPTFSNSKRMEDAFDVQRDHIRLMTD
LKRLLRKGGTIMFSNNKRGFRMDHDGLAALGLKAQEISQKTLSQDFARNRQIHNCWLITAA

Sequences:

>Translated_701_residues
MNSLFASTARGLEELLKTELEGLGATDCQVVQGGVHFQGDTRLLYQSLMWSRLASRIMLPLGECRVYSDLDLYLGVQAIP
WTEMFNPGATFAVHFSGLNDEIRNSQYGALKVKDAIVDSFTRKNLPRPNVDRESPDLRINVWLNKETAHISLDLSGEGLH
LRGYRDGTGMAPIKENLAAAIVMRSGWVPGTPLLDPMCGSGTLLIEAAMLATDRAPGLHRGHWGFGGWAQHDDAIWKEVK
AEAQTRARQGLAAYESRFYGSDVDVRVIERARRNARRAGIGELVDFDVKDVAQLNNPLPKGPYGTVISNPPYGERLESEP
ALIALHSLLGRIMKSQFGGWNLSVFSASPELLSCLQLRADKQFKAKNGPLDCVQKNYHLAESEGGKPAMLAEDFANRLRK
NLKKFEKWARQEGIECYRLYDADLPEYNVAIDRYADWVVVQEYAPPKTVDAHKARQRLFDIIAATIAVLDMAPNKLVLKT
RERQKGKNQYQKMAEKGDFIEVQEYNARLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSASVHAGLGGA
RSTTTVDMSRTYLEWAERNLRLNGLTGRAHRLMQADVLGWLRESTEQFDLIFIDPPTFSNSKRMEDAFDVQRDHIRLMTD
LKRLLRKGGTIMFSNNKRGFRMDHDGLAALGLKAQEISQKTLSQDFARNRQIHNCWLITAA
>Mature_701_residues
MNSLFASTARGLEELLKTELEGLGATDCQVVQGGVHFQGDTRLLYQSLMWSRLASRIMLPLGECRVYSDLDLYLGVQAIP
WTEMFNPGATFAVHFSGLNDEIRNSQYGALKVKDAIVDSFTRKNLPRPNVDRESPDLRINVWLNKETAHISLDLSGEGLH
LRGYRDGTGMAPIKENLAAAIVMRSGWVPGTPLLDPMCGSGTLLIEAAMLATDRAPGLHRGHWGFGGWAQHDDAIWKEVK
AEAQTRARQGLAAYESRFYGSDVDVRVIERARRNARRAGIGELVDFDVKDVAQLNNPLPKGPYGTVISNPPYGERLESEP
ALIALHSLLGRIMKSQFGGWNLSVFSASPELLSCLQLRADKQFKAKNGPLDCVQKNYHLAESEGGKPAMLAEDFANRLRK
NLKKFEKWARQEGIECYRLYDADLPEYNVAIDRYADWVVVQEYAPPKTVDAHKARQRLFDIIAATIAVLDMAPNKLVLKT
RERQKGKNQYQKMAEKGDFIEVQEYNARLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSASVHAGLGGA
RSTTTVDMSRTYLEWAERNLRLNGLTGRAHRLMQADVLGWLRESTEQFDLIFIDPPTFSNSKRMEDAFDVQRDHIRLMTD
LKRLLRKGGTIMFSNNKRGFRMDHDGLAALGLKAQEISQKTLSQDFARNRQIHNCWLITAA

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=702, Percent_Identity=88.8888888888889, Blast_Score=1289, Evalue=0.0,
Organism=Escherichia coli, GI145693111, Length=244, Percent_Identity=28.6885245901639, Blast_Score=86, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR002296
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 78926; Mature: 78926

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: PS51165 THUMP ; PS00092 N6_MTASE ; PS01261 UPF0020

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSLFASTARGLEELLKTELEGLGATDCQVVQGGVHFQGDTRLLYQSLMWSRLASRIMLP
CCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCEEECCCHHHHHHHHHHHHHHHHHHCC
LGECRVYSDLDLYLGVQAIPWTEMFNPGATFAVHFSGLNDEIRNSQYGALKVKDAIVDSF
CCCCEEECCCHHHCCCEECCHHHHCCCCCEEEEEECCCCHHHCCCCCCEEEHHHHHHHHH
TRKNLPRPNVDRESPDLRINVWLNKETAHISLDLSGEGLHLRGYRDGTGMAPIKENLAAA
HHCCCCCCCCCCCCCCEEEEEEEECCEEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHH
IVMRSGWVPGTPLLDPMCGSGTLLIEAAMLATDRAPGLHRGHWGFGGWAQHDDAIWKEVK
HHHCCCCCCCCCCCCCCCCCCCEEEHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
AEAQTRARQGLAAYESRFYGSDVDVRVIERARRNARRAGIGELVDFDVKDVAQLNNPLPK
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHCCCCCC
GPYGTVISNPPYGERLESEPALIALHSLLGRIMKSQFGGWNLSVFSASPELLSCLQLRAD
CCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHCC
KQFKAKNGPLDCVQKNYHLAESEGGKPAMLAEDFANRLRKNLKKFEKWARQEGIECYRLY
CCCCCCCCCHHHHHCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
DADLPEYNVAIDRYADWVVVQEYAPPKTVDAHKARQRLFDIIAATIAVLDMAPNKLVLKT
CCCCCCCCEEHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEC
RERQKGKNQYQKMAEKGDFIEVQEYNARLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKD
HHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEHHHHCCCHHHHHHHHHHHHHHHHCCHH
FLNLFSYTGSASVHAGLGGARSTTTVDMSRTYLEWAERNLRLNGLTGRAHRLMQADVLGW
HHHHHHCCCCCCEECCCCCCCCCEEEHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHH
LRESTEQFDLIFIDPPTFSNSKRMEDAFDVQRDHIRLMTDLKRLLRKGGTIMFSNNKRGF
HHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCC
RMDHDGLAALGLKAQEISQKTLSQDFARNRQIHNCWLITAA
EECCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEC
>Mature Secondary Structure
MNSLFASTARGLEELLKTELEGLGATDCQVVQGGVHFQGDTRLLYQSLMWSRLASRIMLP
CCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCEEECCCHHHHHHHHHHHHHHHHHHCC
LGECRVYSDLDLYLGVQAIPWTEMFNPGATFAVHFSGLNDEIRNSQYGALKVKDAIVDSF
CCCCEEECCCHHHCCCEECCHHHHCCCCCEEEEEECCCCHHHCCCCCCEEEHHHHHHHHH
TRKNLPRPNVDRESPDLRINVWLNKETAHISLDLSGEGLHLRGYRDGTGMAPIKENLAAA
HHCCCCCCCCCCCCCCEEEEEEEECCEEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHH
IVMRSGWVPGTPLLDPMCGSGTLLIEAAMLATDRAPGLHRGHWGFGGWAQHDDAIWKEVK
HHHCCCCCCCCCCCCCCCCCCCEEEHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
AEAQTRARQGLAAYESRFYGSDVDVRVIERARRNARRAGIGELVDFDVKDVAQLNNPLPK
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHCCCCCC
GPYGTVISNPPYGERLESEPALIALHSLLGRIMKSQFGGWNLSVFSASPELLSCLQLRAD
CCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHCC
KQFKAKNGPLDCVQKNYHLAESEGGKPAMLAEDFANRLRKNLKKFEKWARQEGIECYRLY
CCCCCCCCCHHHHHCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
DADLPEYNVAIDRYADWVVVQEYAPPKTVDAHKARQRLFDIIAATIAVLDMAPNKLVLKT
CCCCCCCCEEHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEC
RERQKGKNQYQKMAEKGDFIEVQEYNARLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKD
HHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEHHHHCCCHHHHHHHHHHHHHHHHCCHH
FLNLFSYTGSASVHAGLGGARSTTTVDMSRTYLEWAERNLRLNGLTGRAHRLMQADVLGW
HHHHHHCCCCCCEECCCCCCCCCEEEHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHH
LRESTEQFDLIFIDPPTFSNSKRMEDAFDVQRDHIRLMTDLKRLLRKGGTIMFSNNKRGF
HHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCC
RMDHDGLAALGLKAQEISQKTLSQDFARNRQIHNCWLITAA
EECCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA