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The map label for this gene is potH [H]

Identifier: 238893915

GI number: 238893915

Start: 1792036

End: 1792989

Strand: Direct

Name: potH [H]

Synonym: KP1_1850

Alternate gene names: 238893915

Gene position: 1792036-1792989 (Clockwise)

Preceding gene: 238893914

Following gene: 238893916

Centisome position: 34.14

GC content: 59.33

Gene sequence:

>954_bases
ATGAATACCGTTGAATCCCCGTCAGGGGCGAAAAAACCGGGCGGCTTCGCGCTGTGGGCGGCGCGCCTGCAGATGGCGCA
TGGCCGCAAGCTGGTGATTGCGCTGCCCTATCTGTGGCTGATCCTGTTGTTTATGCTGCCGTTCCTGATCGTGTTCAAGA
TAAGTCTTGCCGAGATGGCGCGGGCGATCCCTCCCTACACCGAGCTGATGGAGTGGGCGGACGGCCAGCTGACGCTGACG
CTTAACTTTGCCAACTTCCTACAGTTGACTGACGATCCGCTCTATTTTGAAGCTTATCTCCAGTCGCTGCAGGTGGCGGG
TATCTCGACGATCTGCTGCCTGCTGCTGGGCTATCCGCTGGCCTGGGCGGTGGCGCACAGCAAGCCGTCGACGCGTAATA
TTCTGCTGCTGTTGGTGATCCTCCCGTCGTGGACCTCGTTTTTGATCCGCGTCTATGCCTGGATGGGGCTGCTGAAGAGC
AACGGGGTGTTGAATAACTTCCTGCTGTGGTTAGGGGTTATCGATCAGCCGCTGGAGATCCTGCACACCAACCTGGCGGT
GTACATCGGTATCGTGTATGCCTATCTGCCGTTTATGGTGCTGCCTATCTACACCGCGCTGACCCGCATCGACTATTCGC
TGGTGGAGGCCTCGCTGGATCTCGGCGCCCGGCCGCTGAAGACCTTCTTCCAGGTGATCGTCCCGCTCACCAAAGGCGGC
ATTATCGCCGGCTCCATGCTGGTGTTTATCCCGGCGGTAGGCGAGTTCGTGATCCCGGAACTGCTGGGCGGGCCGGACAG
CATCATGATTGGCCGCGTGCTGTGGCAGGAGTTCTTTAACAACCGCGACTGGCCGGTCGCCTCGGCGGTGGCGATTGTCA
TGCTGCTGTTGCTGATCGTGCCGATCATGTGGTTCCACAAGCACCAGCAAAAGCAGATGGGAGAGCAGGGATGA

Upstream 100 bases:

>100_bases
GAACACCGCCATCGGAAAGGGACGCCCACCTGGGGCGATGAGGTGCGTTTATGCTGGGATGCTGACAGCTGCGTTGTGCT
GACGGTGTGAGGAGAGGGCG

Downstream 100 bases:

>100_bases
ACGACTTGCCGGTAGTACGCTCGCCGTGGCGCATTCTGATCCTCGTGCTCGGGTTCACCTTCCTCTACGCCCCGATGCTG
ATGCTGGTGATCTACTCGTT

Product: putrescine transporter subunit: membrane component of ABC superfamily

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MNTVESPSGAKKPGGFALWAARLQMAHGRKLVIALPYLWLILLFMLPFLIVFKISLAEMARAIPPYTELMEWADGQLTLT
LNFANFLQLTDDPLYFEAYLQSLQVAGISTICCLLLGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGLLKS
NGVLNNFLLWLGVIDQPLEILHTNLAVYIGIVYAYLPFMVLPIYTALTRIDYSLVEASLDLGARPLKTFFQVIVPLTKGG
IIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQEFFNNRDWPVASAVAIVMLLLLIVPIMWFHKHQQKQMGEQG

Sequences:

>Translated_317_residues
MNTVESPSGAKKPGGFALWAARLQMAHGRKLVIALPYLWLILLFMLPFLIVFKISLAEMARAIPPYTELMEWADGQLTLT
LNFANFLQLTDDPLYFEAYLQSLQVAGISTICCLLLGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGLLKS
NGVLNNFLLWLGVIDQPLEILHTNLAVYIGIVYAYLPFMVLPIYTALTRIDYSLVEASLDLGARPLKTFFQVIVPLTKGG
IIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQEFFNNRDWPVASAVAIVMLLLLIVPIMWFHKHQQKQMGEQG
>Mature_317_residues
MNTVESPSGAKKPGGFALWAARLQMAHGRKLVIALPYLWLILLFMLPFLIVFKISLAEMARAIPPYTELMEWADGQLTLT
LNFANFLQLTDDPLYFEAYLQSLQVAGISTICCLLLGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGLLKS
NGVLNNFLLWLGVIDQPLEILHTNLAVYIGIVYAYLPFMVLPIYTALTRIDYSLVEASLDLGARPLKTFFQVIVPLTKGG
IIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQEFFNNRDWPVASAVAIVMLLLLIVPIMWFHKHQQKQMGEQG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=317, Percent_Identity=89.2744479495268, Blast_Score=548, Evalue=1e-157,
Organism=Escherichia coli, GI1787369, Length=256, Percent_Identity=44.140625, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1787713, Length=237, Percent_Identity=33.3333333333333, Blast_Score=120, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 35477; Mature: 35477

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS00133 CARBOXYPEPT_ZN_2 ; PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTVESPSGAKKPGGFALWAARLQMAHGRKLVIALPYLWLILLFMLPFLIVFKISLAEMA
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAIPPYTELMEWADGQLTLTLNFANFLQLTDDPLYFEAYLQSLQVAGISTICCLLLGYPL
HHCCCHHHHHHCCCCEEEEEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGLLKSNGVLNNFLLWLGVIDQPLEI
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LHTNLAVYIGIVYAYLPFMVLPIYTALTRIDYSLVEASLDLGARPLKTFFQVIVPLTKGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
IIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQEFFNNRDWPVASAVAIVMLLLLIV
CHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
PIMWFHKHQQKQMGEQG
HHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNTVESPSGAKKPGGFALWAARLQMAHGRKLVIALPYLWLILLFMLPFLIVFKISLAEMA
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAIPPYTELMEWADGQLTLTLNFANFLQLTDDPLYFEAYLQSLQVAGISTICCLLLGYPL
HHCCCHHHHHHCCCCEEEEEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGLLKSNGVLNNFLLWLGVIDQPLEI
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LHTNLAVYIGIVYAYLPFMVLPIYTALTRIDYSLVEASLDLGARPLKTFFQVIVPLTKGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
IIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQEFFNNRDWPVASAVAIVMLLLLIV
CHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
PIMWFHKHQQKQMGEQG
HHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]