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The map label for this gene is ybfF [H]

Identifier: 238893738

GI number: 238893738

Start: 1603495

End: 1604268

Strand: Reverse

Name: ybfF [H]

Synonym: KP1_1662

Alternate gene names: 238893738

Gene position: 1604268-1603495 (Counterclockwise)

Preceding gene: 238893742

Following gene: 238893737

Centisome position: 30.57

GC content: 63.7

Gene sequence:

>774_bases
ATGAAATTAAATAGCCGAGCGCAATCTGCACAGAATCCGCACAACCACTCCCCGATCGTGCTGGTCCACGGTCTGTTTGG
CAGCCTGGACAACCTGGGCATCCTCGCCCGCGACCTGATCGCCGACCATGATATTGTGCAGGTCGATATGCGCAACCACG
GTTTGTCGCCGCGGTCGCCAGAGATGACCTACCCCGCCATGGCGCAGGATCTGCTTGATACGCTCGACGCGCACCAGATC
GAGCGCGCCACCTTTATCGGCCATTCGATGGGCGGGAAAGCGGTAATGGCGCTTACCGCCCTGGCGCCTGAACGCATCAG
CGGTCTGGTGGCGATCGATATCGCGCCGGTGGATTATCACGTTCGCCGCCATGATGAGATCTTCGCCGCTATCCGCGCCG
TCAGCGAATCCGCCGCCAGCACCCGCCAGCAGGCGGCGCAGGTGATGCGCGAGCACCTGCAGGAAGAAGGCGTGATTCAG
TTCCTGCTGAAATCATTCGTCGACGGCGACTGGCGCTTCAACGTGCCGGTGCTGTGGGACCAGTATCCGCATATCGTCGG
CTGGGAAACCATCCCGGCCTGGCCGCACCCGACGCAGTTTATTCCCGGCGGCAACTCGCCCTATGTGACCGACGCCTACC
GCGACGCGCTGCTGGCCCAGTTCCCGCAGGCCCGGGCCCACGTCATCGCCGGCGCCGGACACTGGGTCCATGCCGAGAAA
CCCGAGGCGGTGTTACGCGCCATCCGCCGCTATCTGACGAGCATCGCCGCCTGA

Upstream 100 bases:

>100_bases
GATGGCGGTAAAACTCAGAAAACTTCTGGGTCTTGCAGCCGGATGGCAGAATGTCCAACATAATACCCTAACCCGACGGA
GCGAAAAAAAGAACTTTGCC

Downstream 100 bases:

>100_bases
TTGGCTAAAATATGCCCGACCGCGCCGCAGAATGCCCGCGGTCGTTGGCGCAGCCGTTCGCCTGATGTATGATGGCGCGC
TATCTGCGCCGGCTAGAGCC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MKLNSRAQSAQNPHNHSPIVLVHGLFGSLDNLGILARDLIADHDIVQVDMRNHGLSPRSPEMTYPAMAQDLLDTLDAHQI
ERATFIGHSMGGKAVMALTALAPERISGLVAIDIAPVDYHVRRHDEIFAAIRAVSESAASTRQQAAQVMREHLQEEGVIQ
FLLKSFVDGDWRFNVPVLWDQYPHIVGWETIPAWPHPTQFIPGGNSPYVTDAYRDALLAQFPQARAHVIAGAGHWVHAEK
PEAVLRAIRRYLTSIAA

Sequences:

>Translated_257_residues
MKLNSRAQSAQNPHNHSPIVLVHGLFGSLDNLGILARDLIADHDIVQVDMRNHGLSPRSPEMTYPAMAQDLLDTLDAHQI
ERATFIGHSMGGKAVMALTALAPERISGLVAIDIAPVDYHVRRHDEIFAAIRAVSESAASTRQQAAQVMREHLQEEGVIQ
FLLKSFVDGDWRFNVPVLWDQYPHIVGWETIPAWPHPTQFIPGGNSPYVTDAYRDALLAQFPQARAHVIAGAGHWVHAEK
PEAVLRAIRRYLTSIAA
>Mature_257_residues
MKLNSRAQSAQNPHNHSPIVLVHGLFGSLDNLGILARDLIADHDIVQVDMRNHGLSPRSPEMTYPAMAQDLLDTLDAHQI
ERATFIGHSMGGKAVMALTALAPERISGLVAIDIAPVDYHVRRHDEIFAAIRAVSESAASTRQQAAQVMREHLQEEGVIQ
FLLKSFVDGDWRFNVPVLWDQYPHIVGWETIPAWPHPTQFIPGGNSPYVTDAYRDALLAQFPQARAHVIAGAGHWVHAEK
PEAVLRAIRRYLTSIAA

Specific function: Displays esterase activity toward palmitoyl-CoA, malonyl-CoA and pNP-butyrate [H]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

Organism=Homo sapiens, GI23200012, Length=247, Percent_Identity=35.6275303643725, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI23200008, Length=247, Percent_Identity=35.6275303643725, Blast_Score=143, Evalue=1e-34,
Organism=Homo sapiens, GI223941839, Length=242, Percent_Identity=30.5785123966942, Blast_Score=101, Evalue=6e-22,
Organism=Escherichia coli, GI1786902, Length=254, Percent_Identity=84.6456692913386, Blast_Score=450, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI17508535, Length=255, Percent_Identity=32.5490196078431, Blast_Score=133, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI17507063, Length=251, Percent_Identity=27.8884462151394, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6321468, Length=262, Percent_Identity=32.0610687022901, Blast_Score=104, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24640348, Length=272, Percent_Identity=28.3088235294118, Blast_Score=115, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 28469; Mature: 28469

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00285 POTATO_INHIBITOR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNSRAQSAQNPHNHSPIVLVHGLFGSLDNLGILARDLIADHDIVQVDMRNHGLSPRSP
CCCCCHHHHCCCCCCCCCEEEEEEHHCCCCHHHHHHHHHHCCCCEEEEEHHHCCCCCCCC
EMTYPAMAQDLLDTLDAHQIERATFIGHSMGGKAVMALTALAPERISGLVAIDIAPVDYH
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCHHHHCCEEEEEEECCCHH
VRRHDEIFAAIRAVSESAASTRQQAAQVMREHLQEEGVIQFLLKSFVDGDWRFNVPVLWD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCEEEC
QYPHIVGWETIPAWPHPTQFIPGGNSPYVTDAYRDALLAQFPQARAHVIAGAGHWVHAEK
CCCCEECCCCCCCCCCCCCCCCCCCCCEECHHHHHHHHHHCCHHHHEEEECCCCEECCCC
PEAVLRAIRRYLTSIAA
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLNSRAQSAQNPHNHSPIVLVHGLFGSLDNLGILARDLIADHDIVQVDMRNHGLSPRSP
CCCCCHHHHCCCCCCCCCEEEEEEHHCCCCHHHHHHHHHHCCCCEEEEEHHHCCCCCCCC
EMTYPAMAQDLLDTLDAHQIERATFIGHSMGGKAVMALTALAPERISGLVAIDIAPVDYH
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCHHHHCCEEEEEEECCCHH
VRRHDEIFAAIRAVSESAASTRQQAAQVMREHLQEEGVIQFLLKSFVDGDWRFNVPVLWD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCEEEC
QYPHIVGWETIPAWPHPTQFIPGGNSPYVTDAYRDALLAQFPQARAHVIAGAGHWVHAEK
CCCCEECCCCCCCCCCCCCCCCCCCCCEECHHHHHHHHHHCCHHHHEEEECCCCEECCCC
PEAVLRAIRRYLTSIAA
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10493123 [H]