Click here to switch to the map view.

The map label for this gene is nagE [H]

Identifier: 238893728

GI number: 238893728

Start: 1595006

End: 1596961

Strand: Direct

Name: nagE [H]

Synonym: KP1_1651

Alternate gene names: 238893728

Gene position: 1595006-1596961 (Clockwise)

Preceding gene: 238893722

Following gene: 238893729

Centisome position: 30.39

GC content: 59.82

Gene sequence:

>1956_bases
ATGAATATTTTAGGTTTCTTTCAGCGACTGGGTAGGGCGTTGCAGCTCCCTATCGCAGTGCTGCCGGTCGCGGCATTGCT
GCTGCGTTTCGGGCAACCAGACCTGTTGAATGTACCCTTCATCGCGCAAGCGGGTGGGGCGATCTTCGATAATCTGGCGC
TGATCTTCGCAATTGGTGTGGCATCAAGCTGGTCCAAGGATAACGCCGGTTCTGCGGCGCTGGCCGGTGCGGTAGGGTAT
TTCGTGATGACCAAAGCGATGGTCACCATCAACCCGGAAATCAATATGGGCGTGCTGGCCGGTATCATTACCGGTCTGGT
GGCAGGGGCGGTATACAACCGCTGGGCCGGCATCAAGCTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACGCTTCGTGC
CGATCGCCACCGGCTTCTTCTGTCTGATCCTCGCGGCGATCTTCGGCTACGTCTGGCCGCCGGTGCAGCACGCTATCCAC
TCTGGCGGCGAATGGATCGTCTCCGCAGGGGCGCTGGGTTCCGGTATCTTTGGTTTCATCAACCGTCTGCTGATCCCAAC
CGGTCTGCATCAGGTGCTGAACACCATCGCCTGGTTCCAGATTGGTGAGTTCACTAACGCCGCTGGCGCTGTGTTCCACG
GCGACATCAACCGCTTCTACGCCGGCGACGGCACCGCGGGGATGTTCATGTCCGGCTTCTTCCCAATCATGATGTTTGGT
CTGCCGGGTGCGGCGCTGGCGATGTATCTGGCGGCGCCGAAAGCGCGTCGTCCGATGGTAGGCGGTATGCTGCTGTCCGT
GGCCATCACCGCGTTCCTGACCGGGGTAACCGAGCCGCTGGAATTCCTGTTCATGTTCCTGGCTCCGCTGCTGTACCTCC
TGCATGCGGTGCTGACCGGTATCAGCCTGTTCATCGCAACGGCGCTCGGGATCCATGCGGGCTTCTCCTTCTCCGCAGGT
GCAATTGACTACGTGCTGATGTACAGCCTGCCGGCCGCCAGCAAAAACGTCTGGATGCTGCTGGTGATGGGCGTGGTGTT
CTTCTTCGTCTACTTCCTGCTGTTCAGCGCGGTGATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGACAAAGCCG
CTGACGTGGTAACGGAAGAAGCCAACAGCAACACTGAAGAGGGGCTGACCCAGCTGGCGACCAGCTATATCGCCGCGGTG
GGCGGAACGGACAACCTGAAAGCCATTGACGCCTGTATCACCCGTCTGCGTCTGACCGTGGGCGATTCGGCGAAGGTGAA
CGACGCCGCCTGTAAACGTCTCGGCGCGTCCGGGGTGGTGAAACTGAATAAGCAAACCATTCAGGTTATCGTCGGTGCGA
AAGCAGAGTCTATCGGCGATGAGATGAAAAAAGTGGTTACCCGCGGCCCGGTAGCGGCAGCGGCAGCCGCACCGGCTGGC
AACGTCGCCACCGCAGCGCCCGCGGCTAAACCGCAGGCGGTAGCGAACGCGAAGACCGTTGAGTCGCTGGTTTCGCCGAT
TACCGGTGACGTGGTGGCCCTTGAGCAGGTGCCGGACGAAGCCTTCGCCAGCAAAGCCGTTGGCGACGGGATCGCGGTGA
AACCGACCAGCAACATCGTGGTGGCCCCGGCCGCCGGTACCGTGGTGAAAATTTTCAACACTAACCACGCGTTCTGCCTC
GAAACCAACAACGGTGCGGAAATCGTCGTTCACATGGGTATCGACACCGTGGCGCTGGAAGGCAAGGGCTTCAAACGTCT
GGTTGAAGAGGGCACGGACGTGAAGGCGGGTGAGCCGATTCTGGAAATGGATCTGGACTTCCTCAACGCCAACGCGCGCT
CAATGATCAGTCCGGTCGTCTGCAGCAACAGCGATGACTACAGCGCGCTGGTGATACTGGTGAGCGGTAAGGTCGTTGCT
GGCCAGACGCCGCTGTATGAGATTAAAGGCAAGTAA

Upstream 100 bases:

>100_bases
TTTTCACACACTTTGCCGGTAAGTGATAAAACCGACCTTGTTACACAATAAAAAAGTGGCTTTGAAATGAGCCTCATCGG
GGTCTCATAGGGGGAATAAG

Downstream 100 bases:

>100_bases
GGCTCCTGAGTCGAGTTTTTGCCTCAGCGGCGAAGGGAAACCTTCGCCGCTTTTTTTTCCAGCAATCGGCCCCATAATCC
TCTTCTGATACCTATTTTAC

Product: PTS system N-acetyl glucosamine specific transporter subunits IIABC

Products: NA

Alternate protein names: EIICBA-Nag; EII-Nag; N-acetylglucosamine permease IIC component; PTS system N-acetylglucosamine-specific EIIC component; N-acetylglucosamine-specific phosphotransferase enzyme IIB component; PTS system N-acetylglucosamine-specific EIIB component; N-acetylglucosamine-specific phosphotransferase enzyme IIA component; PTS system N-acetylglucosamine-specific EIIA component [H]

Number of amino acids: Translated: 651; Mature: 651

Protein sequence:

>651_residues
MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVPFIAQAGGAIFDNLALIFAIGVASSWSKDNAGSAALAGAVGY
FVMTKAMVTINPEINMGVLAGIITGLVAGAVYNRWAGIKLPDFLSFFGGKRFVPIATGFFCLILAAIFGYVWPPVQHAIH
SGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGAVFHGDINRFYAGDGTAGMFMSGFFPIMMFG
LPGAALAMYLAAPKARRPMVGGMLLSVAITAFLTGVTEPLEFLFMFLAPLLYLLHAVLTGISLFIATALGIHAGFSFSAG
AIDYVLMYSLPAASKNVWMLLVMGVVFFFVYFLLFSAVIRMFNLKTPGREDKAADVVTEEANSNTEEGLTQLATSYIAAV
GGTDNLKAIDACITRLRLTVGDSAKVNDAACKRLGASGVVKLNKQTIQVIVGAKAESIGDEMKKVVTRGPVAAAAAAPAG
NVATAAPAAKPQAVANAKTVESLVSPITGDVVALEQVPDEAFASKAVGDGIAVKPTSNIVVAPAAGTVVKIFNTNHAFCL
ETNNGAEIVVHMGIDTVALEGKGFKRLVEEGTDVKAGEPILEMDLDFLNANARSMISPVVCSNSDDYSALVILVSGKVVA
GQTPLYEIKGK

Sequences:

>Translated_651_residues
MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVPFIAQAGGAIFDNLALIFAIGVASSWSKDNAGSAALAGAVGY
FVMTKAMVTINPEINMGVLAGIITGLVAGAVYNRWAGIKLPDFLSFFGGKRFVPIATGFFCLILAAIFGYVWPPVQHAIH
SGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGAVFHGDINRFYAGDGTAGMFMSGFFPIMMFG
LPGAALAMYLAAPKARRPMVGGMLLSVAITAFLTGVTEPLEFLFMFLAPLLYLLHAVLTGISLFIATALGIHAGFSFSAG
AIDYVLMYSLPAASKNVWMLLVMGVVFFFVYFLLFSAVIRMFNLKTPGREDKAADVVTEEANSNTEEGLTQLATSYIAAV
GGTDNLKAIDACITRLRLTVGDSAKVNDAACKRLGASGVVKLNKQTIQVIVGAKAESIGDEMKKVVTRGPVAAAAAAPAG
NVATAAPAAKPQAVANAKTVESLVSPITGDVVALEQVPDEAFASKAVGDGIAVKPTSNIVVAPAAGTVVKIFNTNHAFCL
ETNNGAEIVVHMGIDTVALEGKGFKRLVEEGTDVKAGEPILEMDLDFLNANARSMISPVVCSNSDDYSALVILVSGKVVA
GQTPLYEIKGK
>Mature_651_residues
MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVPFIAQAGGAIFDNLALIFAIGVASSWSKDNAGSAALAGAVGY
FVMTKAMVTINPEINMGVLAGIITGLVAGAVYNRWAGIKLPDFLSFFGGKRFVPIATGFFCLILAAIFGYVWPPVQHAIH
SGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGAVFHGDINRFYAGDGTAGMFMSGFFPIMMFG
LPGAALAMYLAAPKARRPMVGGMLLSVAITAFLTGVTEPLEFLFMFLAPLLYLLHAVLTGISLFIATALGIHAGFSFSAG
AIDYVLMYSLPAASKNVWMLLVMGVVFFFVYFLLFSAVIRMFNLKTPGREDKAADVVTEEANSNTEEGLTQLATSYIAAV
GGTDNLKAIDACITRLRLTVGDSAKVNDAACKRLGASGVVKLNKQTIQVIVGAKAESIGDEMKKVVTRGPVAAAAAAPAG
NVATAAPAAKPQAVANAKTVESLVSPITGDVVALEQVPDEAFASKAVGDGIAVKPTSNIVVAPAAGTVVKIFNTNHAFCL
ETNNGAEIVVHMGIDTVALEGKGFKRLVEEGTDVKAGEPILEMDLDFLNANARSMISPVVCSNSDDYSALVILVSGKVVA
GQTPLYEIKGK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786894, Length=649, Percent_Identity=88.7519260400616, Blast_Score=1103, Evalue=0.0,
Organism=Escherichia coli, GI1787343, Length=485, Percent_Identity=42.0618556701031, Blast_Score=378, Evalue=1e-106,
Organism=Escherichia coli, GI1787908, Length=510, Percent_Identity=33.921568627451, Blast_Score=259, Evalue=5e-70,
Organism=Escherichia coli, GI1788757, Length=151, Percent_Identity=52.317880794702, Blast_Score=161, Evalue=1e-40,
Organism=Escherichia coli, GI1790159, Length=166, Percent_Identity=36.144578313253, Blast_Score=110, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001127
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010974 [H]

Pfam domain/function: PF00358 PTS_EIIA_1; PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 68169; Mature: 68169

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00371 PTS_EIIA_TYPE_1_HIS ; PS51093 PTS_EIIA_TYPE_1 ; PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVPFIAQAGGAIFDNLALIFAIGV
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHH
ASSWSKDNAGSAALAGAVGYFVMTKAMVTINPEINMGVLAGIITGLVAGAVYNRWAGIKL
CCCCCCCCCCCHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
PDFLSFFGGKRFVPIATGFFCLILAAIFGYVWPPVQHAIHSGGEWIVSAGALGSGIFGFI
HHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEECCCCCCHHHHHH
NRLLIPTGLHQVLNTIAWFQIGEFTNAAGAVFHGDINRFYAGDGTAGMFMSGFFPIMMFG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEECCCCCHHHHHHHHHHHHHHC
LPGAALAMYLAAPKARRPMVGGMLLSVAITAFLTGVTEPLEFLFMFLAPLLYLLHAVLTG
CCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
ISLFIATALGIHAGFSFSAGAIDYVLMYSLPAASKNVWMLLVMGVVFFFVYFLLFSAVIR
HHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MFNLKTPGREDKAADVVTEEANSNTEEGLTQLATSYIAAVGGTDNLKAIDACITRLRLTV
HHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEE
GDSAKVNDAACKRLGASGVVKLNKQTIQVIVGAKAESIGDEMKKVVTRGPVAAAAAAPAG
CCCCCCCHHHHHHCCCCCEEEECCEEEEEEECCCHHHHHHHHHHHHHCCCCHHHHCCCCC
NVATAAPAAKPQAVANAKTVESLVSPITGDVVALEQVPDEAFASKAVGDGIAVKPTSNIV
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEHHHCCCHHHHHHHCCCCEEECCCCCEE
VAPAAGTVVKIFNTNHAFCLETNNGAEIVVHMGIDTVALEGKGFKRLVEEGTDVKAGEPI
EEECCCCEEEEEECCCEEEEECCCCCEEEEEECCCEEEECCCCHHHHHHCCCCCCCCCCH
LEMDLDFLNANARSMISPVVCSNSDDYSALVILVSGKVVAGQTPLYEIKGK
HHHHHHHHCCCHHHHHCCHHCCCCCCCEEEEEEEECEEEECCCCCEEECCC
>Mature Secondary Structure
MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVPFIAQAGGAIFDNLALIFAIGV
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHH
ASSWSKDNAGSAALAGAVGYFVMTKAMVTINPEINMGVLAGIITGLVAGAVYNRWAGIKL
CCCCCCCCCCCHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
PDFLSFFGGKRFVPIATGFFCLILAAIFGYVWPPVQHAIHSGGEWIVSAGALGSGIFGFI
HHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEECCCCCCHHHHHH
NRLLIPTGLHQVLNTIAWFQIGEFTNAAGAVFHGDINRFYAGDGTAGMFMSGFFPIMMFG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEECCCCCHHHHHHHHHHHHHHC
LPGAALAMYLAAPKARRPMVGGMLLSVAITAFLTGVTEPLEFLFMFLAPLLYLLHAVLTG
CCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
ISLFIATALGIHAGFSFSAGAIDYVLMYSLPAASKNVWMLLVMGVVFFFVYFLLFSAVIR
HHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MFNLKTPGREDKAADVVTEEANSNTEEGLTQLATSYIAAVGGTDNLKAIDACITRLRLTV
HHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEE
GDSAKVNDAACKRLGASGVVKLNKQTIQVIVGAKAESIGDEMKKVVTRGPVAAAAAAPAG
CCCCCCCHHHHHHCCCCCEEEECCEEEEEEECCCHHHHHHHHHHHHHCCCCHHHHCCCCC
NVATAAPAAKPQAVANAKTVESLVSPITGDVVALEQVPDEAFASKAVGDGIAVKPTSNIV
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEHHHCCCHHHHHHHCCCCEEECCCCCEE
VAPAAGTVVKIFNTNHAFCLETNNGAEIVVHMGIDTVALEGKGFKRLVEEGTDVKAGEPI
EEECCCCEEEEEECCCEEEEECCCCCEEEEEECCCEEEECCCCHHHHHHCCCCCCCCCCH
LEMDLDFLNANARSMISPVVCSNSDDYSALVILVSGKVVAGQTPLYEIKGK
HHHHHHHHCCCHHHHHCCHHCCCCCCCEEEEEEEECEEEECCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1745234 [H]