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The map label for this gene is miaB [H]

Identifier: 238893721

GI number: 238893721

Start: 1584701

End: 1586311

Strand: Reverse

Name: miaB [H]

Synonym: KP1_1643

Alternate gene names: 238893721

Gene position: 1586311-1584701 (Counterclockwise)

Preceding gene: 238893723

Following gene: 238893720

Centisome position: 30.22

GC content: 55.18

Gene sequence:

>1611_bases
TTGTCATGGCTATTATCCTTAAAGCAGAATCCGTTAAGTTTACCGGATTTTTCGCCCTCTTGCGGCGATAACGCTCGCAC
TGGTCAGGGGGCGACCAAAGCATTACAATACGCGCCCTGGAATGCGAAATTCACCCTGTATATATCCCCGCGTTTTGGAT
ATCTATTACTGAGCATGAGCAAGTCAATGACAAAAAAACTCCATATTAAAACCTGGGGCTGTCAGATGAACGAGTATGAT
TCATCAAAGATGGCCGATCTGCTGGATGCGACACATGGGTATCAACTGACCGAAGTGGCGGAAGAAGCAGATGTGCTGTT
GCTCAATACCTGCTCAATTCGTGAGAAGGCCCAGGAAAAAGTTTTCCATCAGCTTGGCCGCTGGAAGCTGCTGAAAGAGA
AGAACCCGGACCTGATTATTGGCGTCGGCGGCTGCGTGGCTTCCCAGGAAGGCGACCATATTCGCCAGCGCGCGCACTAT
GTCGATATTATTTTCGGGCCGCAAACCCTGCACCGCCTGCCGGAGATGATTAACTCCGTGCGCGGCAACCGCAGTCCGGT
AGTTGATATCAGCTTCCCGGAAATTGAAAAATTCGATCGCCTGCCAGAGCCGCGCGCCGAAGGGCCGACCGCCTTTGTCT
CGATTATGGAAGGTTGCAATAAATACTGCACCTACTGCGTGGTGCCGTATACCCGCGGAGAAGAAGTTAGCCGTCCGTGC
GACGATATCCTGTTTGAAATCGCCCAGCTGGCGGCGCAGGGCGTGCGTGAGGTCAATCTGCTGGGGCAGAACGTTAACGC
CTGGCGCGGTGAGAATTACGACGGCACCACCGGCAGCTTTGCCGACCTGCTGCGCCTGGTCGCCGCCATCGACGGCATCG
ACCGCATTCGCTTCACCACCAGCCACCCGATCGAATTTACCGACGATATCATCGACGTTTATCGCGATACGCCGGAGCTG
GTGAGCTTCCTGCACCTGCCGGTGCAGAGTGGCTCCGACCGTGTGCTGAACCTGATGGGACGCACCCATACCGCGCTGGA
GTATAAAGCGATTATTCGCAAGCTGCGTGAAGCGCGCCCGGATATTCAGATAAGCTCCGACTTTATCGTCGGCTTCCCGG
GCGAAACCACGGAAGATTTCGAAAAGACCATGAAGCTCATCGCCGACGTCAATTTCGATATGAGCTACAGCTTTATCTTC
TCCGCTCGCCCGGGCACCCCGGCGGCCGATATGGTCGACGACGTGCCGGAGGCGGACAAAAAACAGCGTCTGTATATCCT
GCAGGAACGCATTAACCAGCAGGCGATGGCCTGGAGCCGTCGCATGCTCGGCACGGTACAGCGCATCCTGGTGGAAGGCA
CCTCGCGTAAGAACATCATGGAGCTTTCCGGACGTACGGAGAATAACCGGGTGGTCAACTTCGAAGGTACCCCGGACCTC
GTCGGTAAGTTTGTCGATGTCGAAATCGTCGATGTATACACCAACTCGCTGCGCGGTAAAATCGTGCGCACCGAAGCGGA
GATGGGTCTGCGTATCGCTGAATCACCCGAATCAGTGATTGCCCGCACCCGTAAAGAGAACGATCTTGGCGTGGGAATTT
ACCAGCCATGA

Upstream 100 bases:

>100_bases
TTCTCAAGCACCGTCACGGTAAATCCCTGTTGCGCCAGCCCTAACGCCAGGGCGCCGCCCACCATGCCGCCGCCCACGAT
GGCGACATCTGTTACGTGAA

Downstream 100 bases:

>100_bases
TCCCTTCAGGCCTGTCGGTTCCGGCAGGCCTTGTAATTCCTCTCTCCGTCCCTATATCTACAGCAATGCTTGCGCCATTT
TCCTGTGACGTAAATAATTT

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 536; Mature: 535

Protein sequence:

>536_residues
MSWLLSLKQNPLSLPDFSPSCGDNARTGQGATKALQYAPWNAKFTLYISPRFGYLLLSMSKSMTKKLHIKTWGCQMNEYD
SSKMADLLDATHGYQLTEVAEEADVLLLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGDHIRQRAHY
VDIIFGPQTLHRLPEMINSVRGNRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCTYCVVPYTRGEEVSRPC
DDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSFADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIDVYRDTPEL
VSFLHLPVQSGSDRVLNLMGRTHTALEYKAIIRKLREARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSYSFIF
SARPGTPAADMVDDVPEADKKQRLYILQERINQQAMAWSRRMLGTVQRILVEGTSRKNIMELSGRTENNRVVNFEGTPDL
VGKFVDVEIVDVYTNSLRGKIVRTEAEMGLRIAESPESVIARTRKENDLGVGIYQP

Sequences:

>Translated_536_residues
MSWLLSLKQNPLSLPDFSPSCGDNARTGQGATKALQYAPWNAKFTLYISPRFGYLLLSMSKSMTKKLHIKTWGCQMNEYD
SSKMADLLDATHGYQLTEVAEEADVLLLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGDHIRQRAHY
VDIIFGPQTLHRLPEMINSVRGNRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCTYCVVPYTRGEEVSRPC
DDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSFADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIDVYRDTPEL
VSFLHLPVQSGSDRVLNLMGRTHTALEYKAIIRKLREARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSYSFIF
SARPGTPAADMVDDVPEADKKQRLYILQERINQQAMAWSRRMLGTVQRILVEGTSRKNIMELSGRTENNRVVNFEGTPDL
VGKFVDVEIVDVYTNSLRGKIVRTEAEMGLRIAESPESVIARTRKENDLGVGIYQP
>Mature_535_residues
SWLLSLKQNPLSLPDFSPSCGDNARTGQGATKALQYAPWNAKFTLYISPRFGYLLLSMSKSMTKKLHIKTWGCQMNEYDS
SKMADLLDATHGYQLTEVAEEADVLLLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGDHIRQRAHYV
DIIFGPQTLHRLPEMINSVRGNRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCTYCVVPYTRGEEVSRPCD
DILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSFADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIDVYRDTPELV
SFLHLPVQSGSDRVLNLMGRTHTALEYKAIIRKLREARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSYSFIFS
ARPGTPAADMVDDVPEADKKQRLYILQERINQQAMAWSRRMLGTVQRILVEGTSRKNIMELSGRTENNRVVNFEGTPDLV
GKFVDVEIVDVYTNSLRGKIVRTEAEMGLRIAESPESVIARTRKENDLGVGIYQP

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872782, Length=489, Percent_Identity=36.40081799591, Blast_Score=290, Evalue=3e-78,
Organism=Homo sapiens, GI28872784, Length=478, Percent_Identity=36.8200836820084, Blast_Score=287, Evalue=2e-77,
Organism=Homo sapiens, GI93277076, Length=457, Percent_Identity=30.8533916849015, Blast_Score=181, Evalue=1e-45,
Organism=Escherichia coli, GI1786882, Length=474, Percent_Identity=95.7805907172996, Blast_Score=951, Evalue=0.0,
Organism=Escherichia coli, GI1787057, Length=413, Percent_Identity=28.0871670702179, Blast_Score=152, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI17553146, Length=462, Percent_Identity=32.4675324675325, Blast_Score=242, Evalue=3e-64,
Organism=Caenorhabditis elegans, GI71996771, Length=293, Percent_Identity=29.3515358361775, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI21356207, Length=434, Percent_Identity=37.5576036866359, Blast_Score=278, Evalue=1e-74,
Organism=Drosophila melanogaster, GI19922432, Length=463, Percent_Identity=29.5896328293737, Blast_Score=165, Evalue=8e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 60404; Mature: 60272

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWLLSLKQNPLSLPDFSPSCGDNARTGQGATKALQYAPWNAKFTLYISPRFGYLLLSMS
CCCEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHEECCCCCEEEEEECCCCCHHHHHHH
KSMTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTEVAEEADVLLLNTCSIREKAQEK
HHHHHEEEEEEECCCCCCCCHHHHHHHHHHHCCCCHHHHHCCCCEEEEECCHHHHHHHHH
VFHQLGRWKLLKEKNPDLIIGVGGCVASQEGDHIRQRAHYVDIIFGPQTLHRLPEMINSV
HHHHHCCEEEECCCCCCEEEECCCEECCCCCHHHHHHCCEEEEEECHHHHHHHHHHHHHH
RGNRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCTYCVVPYTRGEEVSRPC
CCCCCCEEEECCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCH
DDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSFADLLRLVAAIDGIDRIRFTT
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHEEEEC
SHPIEFTDDIIDVYRDTPELVSFLHLPVQSGSDRVLNLMGRTHTALEYKAIIRKLREARP
CCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCC
DIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEADK
CEEECCCEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHCCCCCCH
KQRLYILQERINQQAMAWSRRMLGTVQRILVEGTSRKNIMELSGRTENNRVVNFEGTPDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEECCCCCCCEEEEECCCHHH
VGKFVDVEIVDVYTNSLRGKIVRTEAEMGLRIAESPESVIARTRKENDLGVGIYQP
HHHHHCEEEEEEHHHCCCCEEEEEHHHCCCEEECCCHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SWLLSLKQNPLSLPDFSPSCGDNARTGQGATKALQYAPWNAKFTLYISPRFGYLLLSMS
CCEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHEECCCCCEEEEEECCCCCHHHHHHH
KSMTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTEVAEEADVLLLNTCSIREKAQEK
HHHHHEEEEEEECCCCCCCCHHHHHHHHHHHCCCCHHHHHCCCCEEEEECCHHHHHHHHH
VFHQLGRWKLLKEKNPDLIIGVGGCVASQEGDHIRQRAHYVDIIFGPQTLHRLPEMINSV
HHHHHCCEEEECCCCCCEEEECCCEECCCCCHHHHHHCCEEEEEECHHHHHHHHHHHHHH
RGNRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCTYCVVPYTRGEEVSRPC
CCCCCCEEEECCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCH
DDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSFADLLRLVAAIDGIDRIRFTT
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHEEEEC
SHPIEFTDDIIDVYRDTPELVSFLHLPVQSGSDRVLNLMGRTHTALEYKAIIRKLREARP
CCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCC
DIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEADK
CEEECCCEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHCCCCCCH
KQRLYILQERINQQAMAWSRRMLGTVQRILVEGTSRKNIMELSGRTENNRVVNFEGTPDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEECCCCCCCEEEEECCCHHH
VGKFVDVEIVDVYTNSLRGKIVRTEAEMGLRIAESPESVIARTRKENDLGVGIYQP
HHHHHCEEEEEEHHHCCCCEEEEEHHHCCCEEECCCHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA