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The map label for this gene is purR [H]

Identifier: 238893663

GI number: 238893663

Start: 1524062

End: 1525189

Strand: Reverse

Name: purR [H]

Synonym: KP1_1581

Alternate gene names: 238893663

Gene position: 1525189-1524062 (Counterclockwise)

Preceding gene: 238893664

Following gene: 238893662

Centisome position: 29.06

GC content: 63.83

Gene sequence:

>1128_bases
GTGAGGTGGAGCACATCCGCTACCCGGCATAGGGCGCAAGGCGCTGCGCGACGACGCCTTTCTGTGATACCTTTTCAGGC
AGTGTGCGTGACCGACGCCCGCCCGGGCGTCGGCTTCCTGGAAAACAGACGGCGCGAAACGACGATGAGCGATAAAAAAT
GGATTAAGGCCGAGGACGTGGCCAGACTGGCCGGGGTCTCGCGCTCGGCGGTCTCCCGCACCTTCACCCCCGGCGCCTCG
GTGTCGGAGAAGACCCGGCAGAAGGTGCTGAGCGCCGCGGAAGCCCTGGGCTATCAGGTGAATATCATCGCCCGGACGAT
GATCACCGGCAGCAGTAATTTTATCGGCATCGTCACCGCCGGTTTCGACAACCCGTTTCGCAGCAAACTGCTGGCGCCGC
TGGTCCATCAGCTGGCGCTCAATGGCTTTATGCCGCTGCTGATGAACGCTGACGATCCGCAGCAGCTCGCCCCCTCGCTT
AAACTACTGCTGAGCTATCACGTCGCCGGGGTGATCATCACCTCCGGCGCGCCGCCGCTGTCGCTGGCGGAAGAGTATCT
CGCGCGCAAGATCCCCGTCACCCTGATCAACCGCCATGCGGACCTCGCCGGCTGCGACCGGGTGTGCAGCGATAACGCCC
AGGGAGCGAAGCTGGTGGCGGATCTGTTCAGCCGCCGAGGCTGGCGGCAGGTTGGCTTTATTGGCGAAAATCGCGAGAAC
TTCAGCACCCGCCAGCGCTATGAGGCTTTTATCGCCCGGACGTCGGATATGGCGGTGAGCAGCCGCTTCTGCGACGGCGG
CGGCTATCAGGCCGGCTACCAGGCTGCCAGAGAATTGGTTGCTGAAAACCCCGGGATGCAGGCGCTGTTTTGCGCCACCG
ATATGCTGGCGCTGGGCGCCCAGGACGGCCTGCGGGACGCCGCCGCCCCGCTGCCGGCCATCGTCGGCTTTGACGATATC
CCGCAGGCGGACTGGCAGCCCTATCAGCTCACCACCGTTCAGCAGAATACCGCCCTGCTGGCCCACCACGCGGTGGATTT
GTTAATGACGCGGATCGCCCGCTTCAGCCTGCCCTCGCGCCATCGCGAAGTGCCGGTGAAACTAATTATTCGCCATAGCG
CCAAATGA

Upstream 100 bases:

>100_bases
GCGAACCTGCTGATCCTCGATGCTGAAAACGACTACGAGGCCGTCCGCCGTCAGGCACGGGTGCTGACCTCCATTCGCCA
CGGCAGGGTGATTTTGCAGC

Downstream 100 bases:

>100_bases
AAAAATCATTTTATGCTGGAGCGATCACAGAATCTTCTGCGAAACCCGGCCACACTCATTCGGCCCTTCGGGGCTGTTTT
TTTCACGCAAAATGCACACG

Product: putative periplasmic binding protein/LacI transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MRWSTSATRHRAQGAARRRLSVIPFQAVCVTDARPGVGFLENRRRETTMSDKKWIKAEDVARLAGVSRSAVSRTFTPGAS
VSEKTRQKVLSAAEALGYQVNIIARTMITGSSNFIGIVTAGFDNPFRSKLLAPLVHQLALNGFMPLLMNADDPQQLAPSL
KLLLSYHVAGVIITSGAPPLSLAEEYLARKIPVTLINRHADLAGCDRVCSDNAQGAKLVADLFSRRGWRQVGFIGENREN
FSTRQRYEAFIARTSDMAVSSRFCDGGGYQAGYQAARELVAENPGMQALFCATDMLALGAQDGLRDAAAPLPAIVGFDDI
PQADWQPYQLTTVQQNTALLAHHAVDLLMTRIARFSLPSRHREVPVKLIIRHSAK

Sequences:

>Translated_375_residues
MRWSTSATRHRAQGAARRRLSVIPFQAVCVTDARPGVGFLENRRRETTMSDKKWIKAEDVARLAGVSRSAVSRTFTPGAS
VSEKTRQKVLSAAEALGYQVNIIARTMITGSSNFIGIVTAGFDNPFRSKLLAPLVHQLALNGFMPLLMNADDPQQLAPSL
KLLLSYHVAGVIITSGAPPLSLAEEYLARKIPVTLINRHADLAGCDRVCSDNAQGAKLVADLFSRRGWRQVGFIGENREN
FSTRQRYEAFIARTSDMAVSSRFCDGGGYQAGYQAARELVAENPGMQALFCATDMLALGAQDGLRDAAAPLPAIVGFDDI
PQADWQPYQLTTVQQNTALLAHHAVDLLMTRIARFSLPSRHREVPVKLIIRHSAK
>Mature_375_residues
MRWSTSATRHRAQGAARRRLSVIPFQAVCVTDARPGVGFLENRRRETTMSDKKWIKAEDVARLAGVSRSAVSRTFTPGAS
VSEKTRQKVLSAAEALGYQVNIIARTMITGSSNFIGIVTAGFDNPFRSKLLAPLVHQLALNGFMPLLMNADDPQQLAPSL
KLLLSYHVAGVIITSGAPPLSLAEEYLARKIPVTLINRHADLAGCDRVCSDNAQGAKLVADLFSRRGWRQVGFIGENREN
FSTRQRYEAFIARTSDMAVSSRFCDGGGYQAGYQAARELVAENPGMQALFCATDMLALGAQDGLRDAAAPLPAIVGFDDI
PQADWQPYQLTTVQQNTALLAHHAVDLLMTRIARFSLPSRHREVPVKLIIRHSAK

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=328, Percent_Identity=25.9146341463415, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787906, Length=272, Percent_Identity=30.8823529411765, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788474, Length=333, Percent_Identity=28.8288288288288, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787948, Length=328, Percent_Identity=24.0853658536585, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1789202, Length=329, Percent_Identity=27.355623100304, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790689, Length=326, Percent_Identity=24.8466257668712, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790369, Length=344, Percent_Identity=24.4186046511628, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787580, Length=253, Percent_Identity=27.6679841897233, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789456, Length=309, Percent_Identity=26.2135922330097, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1786540, Length=336, Percent_Identity=25, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790715, Length=279, Percent_Identity=24.0143369175627, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1789068, Length=292, Percent_Identity=23.6301369863014, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786268, Length=305, Percent_Identity=23.2786885245902, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI48994940, Length=276, Percent_Identity=24.6376811594203, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 41013; Mature: 41013

Theoretical pI: Translated: 10.29; Mature: 10.29

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRWSTSATRHRAQGAARRRLSVIPFQAVCVTDARPGVGFLENRRRETTMSDKKWIKAEDV
CCCCCHHHHHHHHHHHHHCCEECCEEEEEEECCCCCCCHHHCCCHHCCCCHHHCCCHHHH
ARLAGVSRSAVSRTFTPGASVSEKTRQKVLSAAEALGYQVNIIARTMITGSSNFIGIVTA
HHHHCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEEEEEECCCCEEEEEEE
GFDNPFRSKLLAPLVHQLALNGFMPLLMNADDPQQLAPSLKLLLSYHVAGVIITSGAPPL
CCCCHHHHHHHHHHHHHHHHHCCHHEEECCCCHHHHHHHHHHHHHHHHHEEEEECCCCCH
SLAEEYLARKIPVTLINRHADLAGCDRVCSDNAQGAKLVADLFSRRGWRQVGFIGENREN
HHHHHHHHHCCCHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCEEECCCCCCCCC
FSTRQRYEAFIARTSDMAVSSRFCDGGGYQAGYQAARELVAENPGMQALFCATDMLALGA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEHHHHHHHHHHCC
QDGLRDAAAPLPAIVGFDDIPQADWQPYQLTTVQQNTALLAHHAVDLLMTRIARFSLPSR
CCCHHHHHCCCCHHCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCC
HREVPVKLIIRHSAK
CCCCCEEEEEECCCC
>Mature Secondary Structure
MRWSTSATRHRAQGAARRRLSVIPFQAVCVTDARPGVGFLENRRRETTMSDKKWIKAEDV
CCCCCHHHHHHHHHHHHHCCEECCEEEEEEECCCCCCCHHHCCCHHCCCCHHHCCCHHHH
ARLAGVSRSAVSRTFTPGASVSEKTRQKVLSAAEALGYQVNIIARTMITGSSNFIGIVTA
HHHHCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEEEEEECCCCEEEEEEE
GFDNPFRSKLLAPLVHQLALNGFMPLLMNADDPQQLAPSLKLLLSYHVAGVIITSGAPPL
CCCCHHHHHHHHHHHHHHHHHCCHHEEECCCCHHHHHHHHHHHHHHHHHEEEEECCCCCH
SLAEEYLARKIPVTLINRHADLAGCDRVCSDNAQGAKLVADLFSRRGWRQVGFIGENREN
HHHHHHHHHCCCHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCEEECCCCCCCCC
FSTRQRYEAFIARTSDMAVSSRFCDGGGYQAGYQAARELVAENPGMQALFCATDMLALGA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEHHHHHHHHHHCC
QDGLRDAAAPLPAIVGFDDIPQADWQPYQLTTVQQNTALLAHHAVDLLMTRIARFSLPSR
CCCHHHHHCCCCHHCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCC
HREVPVKLIIRHSAK
CCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA