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The map label for this gene is puuB [H]

Identifier: 238893557

GI number: 238893557

Start: 1402511

End: 1403812

Strand: Reverse

Name: puuB [H]

Synonym: KP1_1462

Alternate gene names: 238893557

Gene position: 1403812-1402511 (Counterclockwise)

Preceding gene: 238893565

Following gene: 238893556

Centisome position: 26.75

GC content: 58.76

Gene sequence:

>1302_bases
ATGAACATCAAGATCGATGCGCTGAACTACTACGGCGCGACGAAGAAATATCACCTCCATTTCCCGGCGCTACGGGAAGA
TATTGAAGCCGATGTGGTAATTATCGGCGGCGGCTTCTCTGGCATTAACACCGCCCTTGAGCTGGCGGAACAGGGGATCA
CCAACGTGGTGGTGCTGGAGGCGCGCCATCTCGGCTACGGCGGCACCGGGCGCAACGGCGGCCAGGTGATGGCCGGTATC
GGCCACGATATCGAAGCGGTAAAAAAGCACGTCGGCAAAGAGGGGCTGGAGACGCTGTTTAAAATCGCCAACCTCGGCGC
GGGCATCATTCGCGAACGCATCCGCAAATACAACATCGACGCCGATTTCGTGCCCGGCTACGGCTATCTTGCCTATAACC
AGCGACAGCTTAAAACGCTGCGCCAGTGGGAGAAGGAGTTTAAAGCCGCTACCCCGGATGAAGAGATCGAACTGTATACC
GGAAAAGAGGTGCAGCAGGTGGTGGGCTCCGAGGTCTACTGCGGCGCGCTGAAACATATGGGCGGCGGCCAGATCCATTC
ACTCAATATGCTGCTCGGTTCGGCGCAGGCCGCCCATTCGCTGGGGGTGAAGATTTTCGAATCCTCGCCGGTGGTGGAAG
TGAATTACGGCAAACAGGTGCGGGTACGCACCGCGATGGGCAGCGTCAAAGCCGCCAAACTGCTGTGGGCCTGCGACAGC
TTCCTCAACAATATGGAGCCGGAGATCTACAACAAGACGCTGGTCACTTACTCCTACCAGGTGTCGACCGAGCCGCTCTC
CGATGAACTGATCGAACGCATCAGCCCACTGCGCGGCGCCTTCAGCGATATTCGCCCGGTAATTAACTACTACCGCGTCA
CCCGGGAAAACCGCCTGCTGTTCGGCAGCGCCACCCGCTTTGTGGAGTATACGCCGAACGATTTCGCCGCCTGGAACCGC
ACCTTGCTGGCGGAGGTCTTCCCCTATCTCAGGGATGTGAAGATCGATTTTGCGTGGGGCGGGCCGATGGCCTGTAGCGC
CAATCTGTTCCCGCAGATCGGCACCCTACGCGATCACAACAACGTCTTTTACGTCCAGGGGTACTCCGGCTTCGGCGTCA
CCCCGTCGCACATCGTGTGCAAAATCCTCGCCGAAGGGATCAACGGCGGTTCCGACCGCTATCGCCTGCTGAGCAGCATC
CCCCACGCGACCATCCACGGTCGTGACAGTCTGCGCTTGCTATTAGTCACGGCCGGAAAACTGATGCATCAAACCGCCGG
TTTCTGGAAAGGCCGGAGTTAA

Upstream 100 bases:

>100_bases
CTATCGTTCTAAAGAACGTCATTCTTCCGGACGATATTCAACGCCCGCGCGGCGTGCTGTGATGAGGGTCTTGAGCCAGG
CAAGACCACAGGGTAACGCG

Downstream 100 bases:

>100_bases
CCAGGAGAACGCTATGCACGCTTTTAAACTTAAACACCCGGTACCTGATCTGCAGCCGATCGGCAGCGTCAGCCTGCTCG
GCGCGACGCCGACCGCCGGC

Product: hypothetical protein

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MNIKIDALNYYGATKKYHLHFPALREDIEADVVIIGGGFSGINTALELAEQGITNVVVLEARHLGYGGTGRNGGQVMAGI
GHDIEAVKKHVGKEGLETLFKIANLGAGIIRERIRKYNIDADFVPGYGYLAYNQRQLKTLRQWEKEFKAATPDEEIELYT
GKEVQQVVGSEVYCGALKHMGGGQIHSLNMLLGSAQAAHSLGVKIFESSPVVEVNYGKQVRVRTAMGSVKAAKLLWACDS
FLNNMEPEIYNKTLVTYSYQVSTEPLSDELIERISPLRGAFSDIRPVINYYRVTRENRLLFGSATRFVEYTPNDFAAWNR
TLLAEVFPYLRDVKIDFAWGGPMACSANLFPQIGTLRDHNNVFYVQGYSGFGVTPSHIVCKILAEGINGGSDRYRLLSSI
PHATIHGRDSLRLLLVTAGKLMHQTAGFWKGRS

Sequences:

>Translated_433_residues
MNIKIDALNYYGATKKYHLHFPALREDIEADVVIIGGGFSGINTALELAEQGITNVVVLEARHLGYGGTGRNGGQVMAGI
GHDIEAVKKHVGKEGLETLFKIANLGAGIIRERIRKYNIDADFVPGYGYLAYNQRQLKTLRQWEKEFKAATPDEEIELYT
GKEVQQVVGSEVYCGALKHMGGGQIHSLNMLLGSAQAAHSLGVKIFESSPVVEVNYGKQVRVRTAMGSVKAAKLLWACDS
FLNNMEPEIYNKTLVTYSYQVSTEPLSDELIERISPLRGAFSDIRPVINYYRVTRENRLLFGSATRFVEYTPNDFAAWNR
TLLAEVFPYLRDVKIDFAWGGPMACSANLFPQIGTLRDHNNVFYVQGYSGFGVTPSHIVCKILAEGINGGSDRYRLLSSI
PHATIHGRDSLRLLLVTAGKLMHQTAGFWKGRS
>Mature_433_residues
MNIKIDALNYYGATKKYHLHFPALREDIEADVVIIGGGFSGINTALELAEQGITNVVVLEARHLGYGGTGRNGGQVMAGI
GHDIEAVKKHVGKEGLETLFKIANLGAGIIRERIRKYNIDADFVPGYGYLAYNQRQLKTLRQWEKEFKAATPDEEIELYT
GKEVQQVVGSEVYCGALKHMGGGQIHSLNMLLGSAQAAHSLGVKIFESSPVVEVNYGKQVRVRTAMGSVKAAKLLWACDS
FLNNMEPEIYNKTLVTYSYQVSTEPLSDELIERISPLRGAFSDIRPVINYYRVTRENRLLFGSATRFVEYTPNDFAAWNR
TLLAEVFPYLRDVKIDFAWGGPMACSANLFPQIGTLRDHNNVFYVQGYSGFGVTPSHIVCKILAEGINGGSDRYRLLSSI
PHATIHGRDSLRLLLVTAGKLMHQTAGFWKGRS

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=412, Percent_Identity=35.1941747572816, Blast_Score=265, Evalue=3e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 48000; Mature: 48000

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS00626 RCC1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIKIDALNYYGATKKYHLHFPALREDIEADVVIIGGGFSGINTALELAEQGITNVVVLE
CCEEEEEEEECCCCCEEEEECHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCCCEEEEEE
ARHLGYGGTGRNGGQVMAGIGHDIEAVKKHVGKEGLETLFKIANLGAGIIRERIRKYNID
EECCCCCCCCCCCCEEEEECCCCHHHHHHHHCHHHHHHHHHHHHCCHHHHHHHHHHCCCC
ADFVPGYGYLAYNQRQLKTLRQWEKEFKAATPDEEIELYTGKEVQQVVGSEVYCGALKHM
CCCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHCCHHHHHHHHHC
GGGQIHSLNMLLGSAQAAHSLGVKIFESSPVVEVNYGKQVRVRTAMGSVKAAKLLWACDS
CCCCEEEHHHHHHCHHHHHHCCEEEECCCCEEEECCCCEEEEEEHHCCHHHHHHHHHHHH
FLNNMEPEIYNKTLVTYSYQVSTEPLSDELIERISPLRGAFSDIRPVINYYRVTRENRLL
HHCCCCCHHHCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
FGSATRFVEYTPNDFAAWNRTLLAEVFPYLRDVKIDFAWGGPMACSANLFPQIGTLRDHN
EECCCEEEEECCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCEECCCCCCCCCCEECCC
NVFYVQGYSGFGVTPSHIVCKILAEGINGGSDRYRLLSSIPHATIHGRDSLRLLLVTAGK
CEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEECCCCEEEEEEHHHH
LMHQTAGFWKGRS
HHHHHCCCCCCCC
>Mature Secondary Structure
MNIKIDALNYYGATKKYHLHFPALREDIEADVVIIGGGFSGINTALELAEQGITNVVVLE
CCEEEEEEEECCCCCEEEEECHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCCCEEEEEE
ARHLGYGGTGRNGGQVMAGIGHDIEAVKKHVGKEGLETLFKIANLGAGIIRERIRKYNID
EECCCCCCCCCCCCEEEEECCCCHHHHHHHHCHHHHHHHHHHHHCCHHHHHHHHHHCCCC
ADFVPGYGYLAYNQRQLKTLRQWEKEFKAATPDEEIELYTGKEVQQVVGSEVYCGALKHM
CCCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHCCHHHHHHHHHC
GGGQIHSLNMLLGSAQAAHSLGVKIFESSPVVEVNYGKQVRVRTAMGSVKAAKLLWACDS
CCCCEEEHHHHHHCHHHHHHCCEEEECCCCEEEECCCCEEEEEEHHCCHHHHHHHHHHHH
FLNNMEPEIYNKTLVTYSYQVSTEPLSDELIERISPLRGAFSDIRPVINYYRVTRENRLL
HHCCCCCHHHCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
FGSATRFVEYTPNDFAAWNRTLLAEVFPYLRDVKIDFAWGGPMACSANLFPQIGTLRDHN
EECCCEEEEECCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCEECCCCCCCCCCEECCC
NVFYVQGYSGFGVTPSHIVCKILAEGINGGSDRYRLLSSIPHATIHGRDSLRLLLVTAGK
CEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEECCCCEEEEEEHHHH
LMHQTAGFWKGRS
HHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]