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The map label for this gene is priC [H]

Identifier: 238893437

GI number: 238893437

Start: 1279282

End: 1279836

Strand: Reverse

Name: priC [H]

Synonym: KP1_1325

Alternate gene names: 238893437

Gene position: 1279836-1279282 (Counterclockwise)

Preceding gene: 238893447

Following gene: 238893436

Centisome position: 24.38

GC content: 65.05

Gene sequence:

>555_bases
TTGGCTGGAAGGGAATGGAGAGGTGTGGTGAAAACAGCTCAACTTTTACAAACGCTGAACGATCAGCTCAGCGAGCTGGC
CGCGCTGGTGGCGCCGCTGGCGGAGCACGCGACCCTCAGCCCGCGCTTTGACCGTCAGCTCTTCCACACCCGCAGCACGC
TGATGCAGGCCTATCTGGCGGAAGCCCAACACAACTTTAACCAGCTCCGCCAGGCCGTCGAGCGCCAGCAGCTGCCGCAG
GTGGTCTGGATTGCCGAGCGTCTGGCGGCACAAATCGCCGCGCTGCGCCGTGAAACCGCCACCTGGTCCTTGCGCAGTTG
GGACCACGCGTCGCCAACGTTGAGCCGCTGGCAGCGCCGTCGCCTGCAGCATCAGGAGTATGAGCGCCGCCTGCTGGCGA
TGCGCGACCAACGCCAGCGTCAGCTGGCGCAGGCGACCAGCCTTGACGAGCAGCAGCGGCTGGGGAAAGAAGTTGAAGCC
TACAGCGGCCGGTTGGCGCGCTGCCGACAGGCGCTGGACAAGATTGAAAACGTACTGGCGCGGCTGACGCGCTAA

Upstream 100 bases:

>100_bases
CCAGCGTACCCAGCGCTACCGCCAGCCAGCCGATAATGGTTAAAATAACAGGTGGCATAATGCGAATCGTTATCAAACAG
ATGAGGCTACTGTAGCACAG

Downstream 100 bases:

>100_bases
CAGGAGAGCCCGATGTCGCTGGAAAATGCATCCGATGAGGTCAAACTGGCCGTCGATTTAATCATGTTGCTGGAATCGCA
TCAGATCCCGGCGCAAACCG

Product: primosomal replication protein N''

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 184; Mature: 183

Protein sequence:

>184_residues
MAGREWRGVVKTAQLLQTLNDQLSELAALVAPLAEHATLSPRFDRQLFHTRSTLMQAYLAEAQHNFNQLRQAVERQQLPQ
VVWIAERLAAQIAALRRETATWSLRSWDHASPTLSRWQRRRLQHQEYERRLLAMRDQRQRQLAQATSLDEQQRLGKEVEA
YSGRLARCRQALDKIENVLARLTR

Sequences:

>Translated_184_residues
MAGREWRGVVKTAQLLQTLNDQLSELAALVAPLAEHATLSPRFDRQLFHTRSTLMQAYLAEAQHNFNQLRQAVERQQLPQ
VVWIAERLAAQIAALRRETATWSLRSWDHASPTLSRWQRRRLQHQEYERRLLAMRDQRQRQLAQATSLDEQQRLGKEVEA
YSGRLARCRQALDKIENVLARLTR
>Mature_183_residues
AGREWRGVVKTAQLLQTLNDQLSELAALVAPLAEHATLSPRFDRQLFHTRSTLMQAYLAEAQHNFNQLRQAVERQQLPQV
VWIAERLAAQIAALRRETATWSLRSWDHASPTLSRWQRRRLQHQEYERRLLAMRDQRQRQLAQATSLDEQQRLGKEVEAY
SGRLARCRQALDKIENVLARLTR

Specific function: PriA recognizes a specific hairpin sequence on bacteriophage phi X174 ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase [H]

COG id: COG3923

COG function: function code L; Primosomal replication protein N''

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786673, Length=175, Percent_Identity=59.4285714285714, Blast_Score=195, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010890 [H]

Pfam domain/function: PF07445 priB_priC [H]

EC number: NA

Molecular weight: Translated: 21529; Mature: 21398

Theoretical pI: Translated: 11.54; Mature: 11.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGREWRGVVKTAQLLQTLNDQLSELAALVAPLAEHATLSPRFDRQLFHTRSTLMQAYLA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
EAQHNFNQLRQAVERQQLPQVVWIAERLAAQIAALRRETATWSLRSWDHASPTLSRWQRR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
RLQHQEYERRLLAMRDQRQRQLAQATSLDEQQRLGKEVEAYSGRLARCRQALDKIENVLA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLTR
HHCC
>Mature Secondary Structure 
AGREWRGVVKTAQLLQTLNDQLSELAALVAPLAEHATLSPRFDRQLFHTRSTLMQAYLA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
EAQHNFNQLRQAVERQQLPQVVWIAERLAAQIAALRRETATWSLRSWDHASPTLSRWQRR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
RLQHQEYERRLLAMRDQRQRQLAQATSLDEQQRLGKEVEAYSGRLARCRQALDKIENVLA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLTR
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1856227; 9278503 [H]