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The map label for this gene is acrA [H]

Identifier: 238893432

GI number: 238893432

Start: 1272618

End: 1273811

Strand: Reverse

Name: acrA [H]

Synonym: KP1_1320

Alternate gene names: 238893432

Gene position: 1273811-1272618 (Counterclockwise)

Preceding gene: 238893435

Following gene: 238893431

Centisome position: 24.27

GC content: 56.2

Gene sequence:

>1194_bases
ATGAACAAAAACAGAGGGTTAACGCCTCTGGCGGTCGTTCTGATGCTCTCAGGCAGCTTAGCGCTAACAGGATGTGACGA
TAAACCGGCTCAACAGGGAGCCCAGCACATGCCGGAAGTCGGTATTGTGACGCTCAAATCCGCACCTCTACAAATAACCA
CCGAACTGCCAGGCCGCACCAGCGCCTATCGCATTGCGGAAGTCCGTCCTCAGGTCAGTGGCATTATTTTAAAACGTAAC
TTCGTGGAAGGTAGCGATATCCAGGCCGGCGTCTCTCTGTATCAGATCGATCCAGCCACCTATCAGGCCAGCTATGACAG
CGCCAAAGGCGACCTGGCAAAAGCCCAGGCGGCGGCAAACATGGATCAACTGACGGTCAAGCGTTATCAGAAACTGTTGG
GCACCAAATATATTAGTCAACAAGACTACGATACCGCCGTTGCGACGGCGCAGCAGAGCAATGCGGCCGTGGTCGCGGCG
AAAGCTGCCGTTGAAACCGCGCGCATCAATCTGGCCTACACCAAAGTCACCTCGCCGATCAGCGGCCGGATCGGTAAATC
CGCCGTGACCGAAGGGGCGCTGGTACAGAATGGTCAAACGACCGCCCTGGCAACCGTACAGCAGCTGGATCCGATCTATG
TTGACGTCACCCAGTCGAGCAATGATTTCCTGCGCCTGAAGCAGGAGCTAGCCGACGGCCGCCTGAAACAGGAAAACGGC
AAAGCGAAAGTGGAGCTGGTGACTAATGACGGTCTTAAGTATCCGCAGTCCGGCACGCTGGAATTCTCGGATGTCACCGT
CGATCAGACCACCGGCTCAATCACACTACGCGCTATTTTCCCGAACCCGGATCACACCCTGCTACCGGGGATGTTCGTCC
GTGCCCGTCTGGAAGAAGGGATTAACCCTGACGCCCTGCTGGTACCGCAACAGGGTGTTACCCGTACGCCGCGCGGCGAC
GCCAGCGTCATGGTAGTGGGGGAAGGCGATAAAGTCGAAGTCCGCCAGGTCACTGCTTCTCAGGCGATCGGCGATAAATG
GCTGGTCACTGACGGTCTGAAATCCGGCGATCGCGTTATCGTCACCGGCCTGCAAAAAATCAAACCAGGTGTGCAGGTAA
AAGCGCAGGAAGTAGCTTCTGATGATAAACAGCAAGCCGCAGGCAACGCGCCATCAGAACAAACCAAGTCTTAA

Upstream 100 bases:

>100_bases
AATGTATGTACCATAGCATGACCATAATAGAAAGACTGTAGTGGGTTTGTGGTTGTTTGAGCCACTGAACATTTTGAAAT
TGGACACTCGAGGTTTACAT

Downstream 100 bases:

>100_bases
CTTAAACAGGAGCCGTTAAGACATGCCTAATTTCTTTATCGATCGCCCCATATTTGCATGGGTGATCGCCATTATCATCA
TGCTGGCTGGGGGATTATCG

Product: acriflavine resistance protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MNKNRGLTPLAVVLMLSGSLALTGCDDKPAQQGAQHMPEVGIVTLKSAPLQITTELPGRTSAYRIAEVRPQVSGIILKRN
FVEGSDIQAGVSLYQIDPATYQASYDSAKGDLAKAQAAANMDQLTVKRYQKLLGTKYISQQDYDTAVATAQQSNAAVVAA
KAAVETARINLAYTKVTSPISGRIGKSAVTEGALVQNGQTTALATVQQLDPIYVDVTQSSNDFLRLKQELADGRLKQENG
KAKVELVTNDGLKYPQSGTLEFSDVTVDQTTGSITLRAIFPNPDHTLLPGMFVRARLEEGINPDALLVPQQGVTRTPRGD
ASVMVVGEGDKVEVRQVTASQAIGDKWLVTDGLKSGDRVIVTGLQKIKPGVQVKAQEVASDDKQQAAGNAPSEQTKS

Sequences:

>Translated_397_residues
MNKNRGLTPLAVVLMLSGSLALTGCDDKPAQQGAQHMPEVGIVTLKSAPLQITTELPGRTSAYRIAEVRPQVSGIILKRN
FVEGSDIQAGVSLYQIDPATYQASYDSAKGDLAKAQAAANMDQLTVKRYQKLLGTKYISQQDYDTAVATAQQSNAAVVAA
KAAVETARINLAYTKVTSPISGRIGKSAVTEGALVQNGQTTALATVQQLDPIYVDVTQSSNDFLRLKQELADGRLKQENG
KAKVELVTNDGLKYPQSGTLEFSDVTVDQTTGSITLRAIFPNPDHTLLPGMFVRARLEEGINPDALLVPQQGVTRTPRGD
ASVMVVGEGDKVEVRQVTASQAIGDKWLVTDGLKSGDRVIVTGLQKIKPGVQVKAQEVASDDKQQAAGNAPSEQTKS
>Mature_397_residues
MNKNRGLTPLAVVLMLSGSLALTGCDDKPAQQGAQHMPEVGIVTLKSAPLQITTELPGRTSAYRIAEVRPQVSGIILKRN
FVEGSDIQAGVSLYQIDPATYQASYDSAKGDLAKAQAAANMDQLTVKRYQKLLGTKYISQQDYDTAVATAQQSNAAVVAA
KAAVETARINLAYTKVTSPISGRIGKSAVTEGALVQNGQTTALATVQQLDPIYVDVTQSSNDFLRLKQELADGRLKQENG
KAKVELVTNDGLKYPQSGTLEFSDVTVDQTTGSITLRAIFPNPDHTLLPGMFVRARLEEGINPDALLVPQQGVTRTPRGD
ASVMVVGEGDKVEVRQVTASQAIGDKWLVTDGLKSGDRVIVTGLQKIKPGVQVKAQEVASDDKQQAAGNAPSEQTKS

Specific function: AcrAB is a drug efflux protein with a broad substrate specificity [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=397, Percent_Identity=84.8866498740554, Blast_Score=656, Evalue=0.0,
Organism=Escherichia coli, GI1789665, Length=375, Percent_Identity=68.2666666666667, Blast_Score=512, Evalue=1e-146,
Organism=Escherichia coli, GI1789929, Length=389, Percent_Identity=53.4704370179949, Blast_Score=410, Evalue=1e-116,
Organism=Escherichia coli, GI87082036, Length=365, Percent_Identity=29.041095890411, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI87081796, Length=380, Percent_Identity=23.6842105263158, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42257; Mature: 42257

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKNRGLTPLAVVLMLSGSLALTGCDDKPAQQGAQHMPEVGIVTLKSAPLQITTELPGRT
CCCCCCCCHHHHHHHHCCCEEEECCCCCHHHHHHHHCCCCEEEEEECCCEEEEECCCCCC
SAYRIAEVRPQVSGIILKRNFVEGSDIQAGVSLYQIDPATYQASYDSAKGDLAKAQAAAN
CEEEEEECCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCEECCCCCCCCHHHHHHHHCC
MDQLTVKRYQKLLGTKYISQQDYDTAVATAQQSNAAVVAAKAAVETARINLAYTKVTSPI
HHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEEHHHEEEEEEEEEEEEECCCC
SGRIGKSAVTEGALVQNGQTTALATVQQLDPIYVDVTQSSNDFLRLKQELADGRLKQENG
CCCCCCHHHCCCCEEECCCCCEEHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCEECCCC
KAKVELVTNDGLKYPQSGTLEFSDVTVDQTTGSITLRAIFPNPDHTLLPGMFVRARLEEG
CEEEEEEECCCCCCCCCCCEEEEEEEEECCCCEEEEEEECCCCCCCCCCHHHHHHHHHCC
INPDALLVPQQGVTRTPRGDASVMVVGEGDKVEVRQVTASQAIGDKWLVTDGLKSGDRVI
CCCCEEEECCCCCCCCCCCCCEEEEEECCCCEEEEEEHHHHHCCCCEEEECCCCCCCEEE
VTGLQKIKPGVQVKAQEVASDDKQQAAGNAPSEQTKS
EEEHHHCCCCCEEEHHHHCCCCHHHHCCCCCCCCCCC
>Mature Secondary Structure
MNKNRGLTPLAVVLMLSGSLALTGCDDKPAQQGAQHMPEVGIVTLKSAPLQITTELPGRT
CCCCCCCCHHHHHHHHCCCEEEECCCCCHHHHHHHHCCCCEEEEEECCCEEEEECCCCCC
SAYRIAEVRPQVSGIILKRNFVEGSDIQAGVSLYQIDPATYQASYDSAKGDLAKAQAAAN
CEEEEEECCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCEECCCCCCCCHHHHHHHHCC
MDQLTVKRYQKLLGTKYISQQDYDTAVATAQQSNAAVVAAKAAVETARINLAYTKVTSPI
HHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEEHHHEEEEEEEEEEEEECCCC
SGRIGKSAVTEGALVQNGQTTALATVQQLDPIYVDVTQSSNDFLRLKQELADGRLKQENG
CCCCCCHHHCCCCEEECCCCCEEHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCEECCCC
KAKVELVTNDGLKYPQSGTLEFSDVTVDQTTGSITLRAIFPNPDHTLLPGMFVRARLEEG
CEEEEEEECCCCCCCCCCCEEEEEEEEECCCCEEEEEEECCCCCCCCCCHHHHHHHHHCC
INPDALLVPQQGVTRTPRGDASVMVVGEGDKVEVRQVTASQAIGDKWLVTDGLKSGDRVI
CCCCEEEECCCCCCCCCCCCCEEEEEECCCCEEEEEEHHHHHCCCCEEEECCCCCCCEEE
VTGLQKIKPGVQVKAQEVASDDKQQAAGNAPSEQTKS
EEEHHHCCCCCEEEHHHHCCCCHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]