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The map label for this gene is yhfM [C]

Identifier: 238893289

GI number: 238893289

Start: 1131682

End: 1133061

Strand: Reverse

Name: yhfM [C]

Synonym: KP1_1165

Alternate gene names: 238893289

Gene position: 1133061-1131682 (Counterclockwise)

Preceding gene: 238893297

Following gene: 238893288

Centisome position: 21.59

GC content: 64.42

Gene sequence:

>1380_bases
ATGACAGAGCACACCACCACCTTAAAAAGAACGCTCGGCAGTTTTCGTTTATGGGGGATCGCCGTCGGACTGGTGATATC
CGGGGAGTACTTCGGCTGGAGCTACGGCTGGTCGCAGGCGGGCACGATGGGGTTTATGGTCGTCGCCCTGGCGGTCGCCG
CGATGTACTGCGCCTTTATTTTCAGCTTTACCGAGCTGACCACCGCCATCCCGCACGCTGGCGGCCCTTTCGCCTATGCC
TACCGCGCCTTCGGGCCCACCGGCGGCTTTATCGCTGGTTTTGCCACCCTGATTGAATTCGTCTTCGCCCCGCCGGCCAT
CGCCATGGCCATCGGGGCCTACCTCAACGTGCAGTTTCCGGCGCTGGATCCGAAATGGGTCGCCTGCGGCGCCTACGTGA
TCTTTATGACGCTCAATATTCTCGGCGTCGGCATCGCCGCCACCTTCGAGCTGATCGTCACTCTGCTGGCCATCTTCGAG
CTGCTGGTGTTCATGGGCGTAGTCGCCCCCGGCTTCTCCTGGAGCCACTTCACCACTAACGGCTGGGCGGGCGCGGATAG
CTTCAGCGGCCTGGCGCTGCCGGGGATGTTCGCCGCCATTCCGTTCGCCATCTGGTTCTTCCTCGCCATTGAGGGGGCCT
CCATGGCGGCCGAAGAGGCCAAAGATCCGCAGCGCACCATTCCCCGCGCCCTGGGCGGCGGCATTCTGACGCTGACGGTG
CTGGCGATCGGCGTGATGGTCTTCGCCGGCGGCGTCGGCGACTGGCGCGCGCTGTCCAATATTAACGATCCGCTGCCCCA
GGCGATGAAGACGGTGGTGGGCAACGGCAGCGGCTGGCTGCATATGCTGGTGTGGCTGGGGCTGTTTGGCCTCGTCGCCT
CCTTCCACGGCATCATTATGGGCTACTCGCGGCAGATCTACTCCCTCGCCCGCGCCGGCTATCTGCCCGCCGGGCTGGCC
TCCCTCAACCGCCGCACCCGCACCCCGCATCTGGCGATCCTCGCCGGCGGCGTGGTCGGGATCGCCGCGATCTTCTCCGA
CTCGCTGATCACCATCAGCGGCATGCCGCTGACCGCCTGCATCGTCACCATGTCGGTATTTGGGGCTATTGTTATGTATA
TCACTTCCATGGCGGCGCTGTTCAAACTGCGCCGTAGCGAGCCGAAGCTGATCCGTCCGTTCCGCGCCCCGCTCTACCCG
CTGGCGCCGGCCTTTGCCCTCGGCATGGCGGTGCTCTGCCTGGTGGCGATGGTCTGGTATAACCTGCTGTTGGCGCTGAT
TTTCGCGGCGATGATGCTCGGCGGCTACCTGTGGTTCCGCAAAACCGCAGCGGCCCGTGAACGGGCGCCAGTGGATCCTC
AACTGCGCACGGTCTCCTGA

Upstream 100 bases:

>100_bases
GGGCGCGCCACGGACTGACGCCTTATCGCGGCAGACAACGTTCTGGCACTCTCTTTGCAGCCTGTCGTTCAGCACCTTTC
TATCTGGCGAGGCAATACGT

Downstream 100 bases:

>100_bases
GGAGGCGGCATGTATAAAACCACGCTATCGGGCCAGGTATGGCGCTTTGATAGTCTGAAAACGTTGATGGCGAAAGCGTC
GCCAGCCCGCTCCGGCGACG

Product: putative amino acid/amine transport protein

Products: L-tryptophan [Cytoplasm]; Proton [Cytoplasm]; Proton [Cytoplasm]; L-tyrosine [Cytoplasm]; Proton [Cytoplasm]; L-phenylalanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MTEHTTTLKRTLGSFRLWGIAVGLVISGEYFGWSYGWSQAGTMGFMVVALAVAAMYCAFIFSFTELTTAIPHAGGPFAYA
YRAFGPTGGFIAGFATLIEFVFAPPAIAMAIGAYLNVQFPALDPKWVACGAYVIFMTLNILGVGIAATFELIVTLLAIFE
LLVFMGVVAPGFSWSHFTTNGWAGADSFSGLALPGMFAAIPFAIWFFLAIEGASMAAEEAKDPQRTIPRALGGGILTLTV
LAIGVMVFAGGVGDWRALSNINDPLPQAMKTVVGNGSGWLHMLVWLGLFGLVASFHGIIMGYSRQIYSLARAGYLPAGLA
SLNRRTRTPHLAILAGGVVGIAAIFSDSLITISGMPLTACIVTMSVFGAIVMYITSMAALFKLRRSEPKLIRPFRAPLYP
LAPAFALGMAVLCLVAMVWYNLLLALIFAAMMLGGYLWFRKTAAARERAPVDPQLRTVS

Sequences:

>Translated_459_residues
MTEHTTTLKRTLGSFRLWGIAVGLVISGEYFGWSYGWSQAGTMGFMVVALAVAAMYCAFIFSFTELTTAIPHAGGPFAYA
YRAFGPTGGFIAGFATLIEFVFAPPAIAMAIGAYLNVQFPALDPKWVACGAYVIFMTLNILGVGIAATFELIVTLLAIFE
LLVFMGVVAPGFSWSHFTTNGWAGADSFSGLALPGMFAAIPFAIWFFLAIEGASMAAEEAKDPQRTIPRALGGGILTLTV
LAIGVMVFAGGVGDWRALSNINDPLPQAMKTVVGNGSGWLHMLVWLGLFGLVASFHGIIMGYSRQIYSLARAGYLPAGLA
SLNRRTRTPHLAILAGGVVGIAAIFSDSLITISGMPLTACIVTMSVFGAIVMYITSMAALFKLRRSEPKLIRPFRAPLYP
LAPAFALGMAVLCLVAMVWYNLLLALIFAAMMLGGYLWFRKTAAARERAPVDPQLRTVS
>Mature_458_residues
TEHTTTLKRTLGSFRLWGIAVGLVISGEYFGWSYGWSQAGTMGFMVVALAVAAMYCAFIFSFTELTTAIPHAGGPFAYAY
RAFGPTGGFIAGFATLIEFVFAPPAIAMAIGAYLNVQFPALDPKWVACGAYVIFMTLNILGVGIAATFELIVTLLAIFEL
LVFMGVVAPGFSWSHFTTNGWAGADSFSGLALPGMFAAIPFAIWFFLAIEGASMAAEEAKDPQRTIPRALGGGILTLTVL
AIGVMVFAGGVGDWRALSNINDPLPQAMKTVVGNGSGWLHMLVWLGLFGLVASFHGIIMGYSRQIYSLARAGYLPAGLAS
LNRRTRTPHLAILAGGVVGIAAIFSDSLITISGMPLTACIVTMSVFGAIVMYITSMAALFKLRRSEPKLIRPFRAPLYPL
APAFALGMAVLCLVAMVWYNLLLALIFAAMMLGGYLWFRKTAAARERAPVDPQLRTVS

Specific function: Probable amino-acid or metabolite transport protein [H]

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily [H]

Homologues:

Organism=Homo sapiens, GI7657683, Length=352, Percent_Identity=25.2840909090909, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI4507047, Length=448, Percent_Identity=23.8839285714286, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1786302, Length=473, Percent_Identity=24.7357293868922, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI87082250, Length=381, Percent_Identity=25.4593175853018, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87081708, Length=477, Percent_Identity=23.8993710691824, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI87081869, Length=410, Percent_Identity=25.8536585365854, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1786789, Length=366, Percent_Identity=22.6775956284153, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI87082023, Length=422, Percent_Identity=26.0663507109005, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI2367353, Length=339, Percent_Identity=26.2536873156342, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1786602, Length=406, Percent_Identity=20.4433497536946, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17540018, Length=438, Percent_Identity=26.2557077625571, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17532491, Length=366, Percent_Identity=23.7704918032787, Blast_Score=69, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI71982612, Length=274, Percent_Identity=23.7226277372263, Blast_Score=65, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI71982608, Length=274, Percent_Identity=23.7226277372263, Blast_Score=65, Evalue=8e-11,
Organism=Drosophila melanogaster, GI221331183, Length=363, Percent_Identity=25.068870523416, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17647653, Length=363, Percent_Identity=25.068870523416, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24664379, Length=363, Percent_Identity=25.068870523416, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 49002; Mature: 48871

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEHTTTLKRTLGSFRLWGIAVGLVISGEYFGWSYGWSQAGTMGFMVVALAVAAMYCAFI
CCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
FSFTELTTAIPHAGGPFAYAYRAFGPTGGFIAGFATLIEFVFAPPAIAMAIGAYLNVQFP
HHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHEEECC
ALDPKWVACGAYVIFMTLNILGVGIAATFELIVTLLAIFELLVFMGVVAPGFSWSHFTTN
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
GWAGADSFSGLALPGMFAAIPFAIWFFLAIEGASMAAEEAKDPQRTIPRALGGGILTLTV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHCCHHHHHHH
LAIGVMVFAGGVGDWRALSNINDPLPQAMKTVVGNGSGWLHMLVWLGLFGLVASFHGIIM
HHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
GYSRQIYSLARAGYLPAGLASLNRRTRTPHLAILAGGVVGIAAIFSDSLITISGMPLTAC
HHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEHHHHHHHHHHHCCCCEEECCCHHHHH
IVTMSVFGAIVMYITSMAALFKLRRSEPKLIRPFRAPLYPLAPAFALGMAVLCLVAMVWY
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCHHHCCCCHHHHHHHHHHHHHHHHHHHHH
NLLLALIFAAMMLGGYLWFRKTAAARERAPVDPQLRTVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TEHTTTLKRTLGSFRLWGIAVGLVISGEYFGWSYGWSQAGTMGFMVVALAVAAMYCAFI
CCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
FSFTELTTAIPHAGGPFAYAYRAFGPTGGFIAGFATLIEFVFAPPAIAMAIGAYLNVQFP
HHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHEEECC
ALDPKWVACGAYVIFMTLNILGVGIAATFELIVTLLAIFELLVFMGVVAPGFSWSHFTTN
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
GWAGADSFSGLALPGMFAAIPFAIWFFLAIEGASMAAEEAKDPQRTIPRALGGGILTLTV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHCCHHHHHHH
LAIGVMVFAGGVGDWRALSNINDPLPQAMKTVVGNGSGWLHMLVWLGLFGLVASFHGIIM
HHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
GYSRQIYSLARAGYLPAGLASLNRRTRTPHLAILAGGVVGIAAIFSDSLITISGMPLTAC
HHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEHHHHHHHHHHHCCCCEEECCCHHHHH
IVTMSVFGAIVMYITSMAALFKLRRSEPKLIRPFRAPLYPLAPAFALGMAVLCLVAMVWY
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCHHHCCCCHHHHHHHHHHHHHHHHHHHHH
NLLLALIFAAMMLGGYLWFRKTAAARERAPVDPQLRTVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-tryptophan [Periplasm]; Proton [Periplasm]; Proton [Periplasm]; L-tyrosine [Periplasm]; Proton [Periplasm]; L-phenylalanine [Periplasm] [C]

Specific reaction: L-tryptophan [Periplasm] + Proton [Periplasm] = L-tryptophan [Cytoplasm] + Proton [Cytoplasm] Proton [Periplasm] + L-tyrosine [Periplasm] = Proton [Cytoplasm] + L-tyrosine [Cytoplasm] Proton [Periplasm] + L-phenylalanine [Periplasm] = Proton [Cytoplasm] +

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]