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The map label for this gene is nqrF

Identifier: 238893221

GI number: 238893221

Start: 1063284

End: 1064507

Strand: Direct

Name: nqrF

Synonym: KP1_1089

Alternate gene names: 238893221

Gene position: 1063284-1064507 (Clockwise)

Preceding gene: 238893220

Following gene: 238893222

Centisome position: 20.26

GC content: 56.05

Gene sequence:

>1224_bases
ATGGAAATTATTCTTGGCGTCGTGATGTTCACGCTGATTGTGCTGGTGCTGTCGGGGCTTATCCTGGCAGCGCGCTCAAA
GCTGGTGAACGCGGGCGACGTGGTTATTGAGATCAATAACGAAGCGGATAAACAGATCCGCACCCCGGCGGGTGACAAAC
TGCTGAACACGCTGTCCAGCAACGGGATCTTCGTCTCCTCCGCCTGCGGCGGCGGCGGCTCCTGCGGCCAGTGTCGGGTA
ACTGTTAAAGAGGGCGGCGGGGATATCCTGCCGACCGAGCTTTCGCATATTACCAAGCGGGATGCCAAAGCCGGTTGCCG
TCTGGCCTGTCAGGTGGCGGTGAAGCAGAACATGAAGATTGAACTGCCGGAAGAGATTTTCGGCGTCAAAAAATGGGAGT
GTGAAGTTATCTCTAACGATAACAAAGCCACTTTCATTAAAGAGCTCAAGCTGCGGATCCCGGAGGGGGAGGTGGTGCCG
TTTCGCGCCGGGGGCTATATTCAGATTGAATGCCCGCCGCATAAAGTGGCCTATGCAGATTTTGATGTTCCCGATGAATA
CCGTTCAGACTGGGATAAGTTCAACCTGTTTCGCTATGTCTCTGAGGTGAAAGAGCCGACCCTGCGCGCCTACTCAATGG
CTAACTATCCGGAAGAGAAGGGCATCATTATGCTCAACGTGCGCATCGCGACGCCGCCGCCGAAGGTCCCGGATGCGCCG
CCGGGGATCATGTCATCCTATATCTGGTCGCTGAAGCCGGGCGATAAGGTGACGATTTCCGGGCCGTTTGGCGAGTTCTT
TGCTAAAGAAACCGACGCCGAGATGGTGTTTATCGGCGGCGGCGCCGGGATGGCGCCGATGCGTTCGCATATTTTCGACC
AGCTCAAGCGCCTGCACAGCACGCGCAAAATCAGCTTCTGGTACGGCGCCCGTTCGCTGCGCGAAATGTTCTACGATGAG
GAGTTTGAACAGCTGGCGCGCGACAATCCAAACTTTACCTTCCACGTTGCGCTCTCCGATCCGCTGCCGGAAGATAACTG
GACCGGGCATACCGGCTTTATCCATAACGTTCTGTACGAGAACTATCTACGCGATCACCCGGCGCCGGAAGACTGCGAGT
TCTACATGTGTGGGCCGCCGGTAATGAACGCCGCGGTAATTAAGATGCTCAAAGATCTGGGCGTCGAGGACGAGAATATT
CTCCTCGACGACTTTGGAGGCTGA

Upstream 100 bases:

>100_bases
CGCCGGTTTGCGCGGGTTAGGGATCACCTTTATCACCACCGGACTAATGGCGCTGGGCTTTATGTCCTTCTCCGGCGTGC
AGCTATAAGGGCGAATAAGT

Downstream 100 bases:

>100_bases
CGATGCTGACGGTATTTATCGCGACCTTTGTGATTTTTGCGCTGGTGATCCTCGGCATGTCGCTGGGCTACCTGGTGAAG
CGCAAGAGCATCCAGGGCAG

Product: Na(+)-translocating NADH-quinone reductase subunit F

Products: @TETR01txt*tetrahydropteridine!; NO3-; NAD(P); Proton [C]

Alternate protein names: Na(+)-NQR subunit F; Na(+)-translocating NQR subunit F; NQR complex subunit F; NQR-1 subunit F

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MEIILGVVMFTLIVLVLSGLILAARSKLVNAGDVVIEINNEADKQIRTPAGDKLLNTLSSNGIFVSSACGGGGSCGQCRV
TVKEGGGDILPTELSHITKRDAKAGCRLACQVAVKQNMKIELPEEIFGVKKWECEVISNDNKATFIKELKLRIPEGEVVP
FRAGGYIQIECPPHKVAYADFDVPDEYRSDWDKFNLFRYVSEVKEPTLRAYSMANYPEEKGIIMLNVRIATPPPKVPDAP
PGIMSSYIWSLKPGDKVTISGPFGEFFAKETDAEMVFIGGGAGMAPMRSHIFDQLKRLHSTRKISFWYGARSLREMFYDE
EFEQLARDNPNFTFHVALSDPLPEDNWTGHTGFIHNVLYENYLRDHPAPEDCEFYMCGPPVMNAAVIKMLKDLGVEDENI
LLDDFGG

Sequences:

>Translated_407_residues
MEIILGVVMFTLIVLVLSGLILAARSKLVNAGDVVIEINNEADKQIRTPAGDKLLNTLSSNGIFVSSACGGGGSCGQCRV
TVKEGGGDILPTELSHITKRDAKAGCRLACQVAVKQNMKIELPEEIFGVKKWECEVISNDNKATFIKELKLRIPEGEVVP
FRAGGYIQIECPPHKVAYADFDVPDEYRSDWDKFNLFRYVSEVKEPTLRAYSMANYPEEKGIIMLNVRIATPPPKVPDAP
PGIMSSYIWSLKPGDKVTISGPFGEFFAKETDAEMVFIGGGAGMAPMRSHIFDQLKRLHSTRKISFWYGARSLREMFYDE
EFEQLARDNPNFTFHVALSDPLPEDNWTGHTGFIHNVLYENYLRDHPAPEDCEFYMCGPPVMNAAVIKMLKDLGVEDENI
LLDDFGG
>Mature_407_residues
MEIILGVVMFTLIVLVLSGLILAARSKLVNAGDVVIEINNEADKQIRTPAGDKLLNTLSSNGIFVSSACGGGGSCGQCRV
TVKEGGGDILPTELSHITKRDAKAGCRLACQVAVKQNMKIELPEEIFGVKKWECEVISNDNKATFIKELKLRIPEGEVVP
FRAGGYIQIECPPHKVAYADFDVPDEYRSDWDKFNLFRYVSEVKEPTLRAYSMANYPEEKGIIMLNVRIATPPPKVPDAP
PGIMSSYIWSLKPGDKVTISGPFGEFFAKETDAEMVFIGGGAGMAPMRSHIFDQLKRLHSTRKISFWYGARSLREMFYDE
EFEQLARDNPNFTFHVALSDPLPEDNWTGHTGFIHNVLYENYLRDHPAPEDCEFYMCGPPVMNAAVIKMLKDLGVEDENI
LLDDFGG

Specific function: NQR complex catalyzes the reduction of ubiquinone-1 to ubiquinol by two successive reactions, coupled with the transport of Na(+) ions from the cytoplasm to the periplasm. The first step is catalyzed by nqrF, which accepts electrons from NADH and reduces

COG id: COG2871

COG function: function code C; Na+-transporting NADH:ubiquinone oxidoreductase, subunit NqrF

Gene ontology:

Cell location: Cell inner membrane (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain

Homologues:

Organism=Escherichia coli, GI1788903, Length=204, Percent_Identity=28.4313725490196, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323552, Length=191, Percent_Identity=26.1780104712042, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6322146, Length=163, Percent_Identity=31.9018404907975, Blast_Score=74, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6323510, Length=154, Percent_Identity=26.6233766233766, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NQRF_KLEP7 (A6T526)

Other databases:

- EMBL:   CP000647
- RefSeq:   YP_001333927.1
- ProteinModelPortal:   A6T526
- SMR:   A6T526
- STRING:   A6T526
- GeneID:   5339136
- GenomeReviews:   CP000647_GR
- KEGG:   kpn:KPN_00244
- eggNOG:   COG2871
- HOGENOM:   HBG680293
- OMA:   VQLECPP
- ProtClustDB:   PRK05464
- HAMAP:   MF_00430
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR001709
- InterPro:   IPR010205
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR017938
- Gene3D:   G3DSA:3.10.20.30
- PRINTS:   PR00371
- TIGRFAMs:   TIGR01941

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1; SSF54292 Ferredoxin; SSF63380 Riboflavin_synthase_like_b-brl

EC number: 1.5.1.34 [C]

Molecular weight: Translated: 45282; Mature: 45282

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00197 2FE2S_FER_1; PS51085 2FE2S_FER_2; PS51384 FAD_FR

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1a33b2dc)-;

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIILGVVMFTLIVLVLSGLILAARSKLVNAGDVVIEINNEADKQIRTPAGDKLLNTLSS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHCCCCHHHHHHHHCC
NGIFVSSACGGGGSCGQCRVTVKEGGGDILPTELSHITKRDAKAGCRLACQVAVKQNMKI
CCEEEEECCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEEHHCCCCE
ELPEEIFGVKKWECEVISNDNKATFIKELKLRIPEGEVVPFRAGGYIQIECPPHKVAYAD
ECCHHHHCCCCEEEEEECCCCCHHEEEHHHHCCCCCCEEEEECCCEEEEECCCCEEEEEC
FDVPDEYRSDWDKFNLFRYVSEVKEPTLRAYSMANYPEEKGIIMLNVRIATPPPKVPDAP
CCCCHHHHCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCC
PGIMSSYIWSLKPGDKVTISGPFGEFFAKETDAEMVFIGGGAGMAPMRSHIFDQLKRLHS
CCHHHHHHEECCCCCEEEEECCCHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHC
TRKISFWYGARSLREMFYDEEFEQLARDNPNFTFHVALSDPLPEDNWTGHTGFIHNVLYE
CCEEEEECCHHHHHHHHCCHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHH
NYLRDHPAPEDCEFYMCGPPVMNAAVIKMLKDLGVEDENILLDDFGG
HHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCEEEECCCH
>Mature Secondary Structure
MEIILGVVMFTLIVLVLSGLILAARSKLVNAGDVVIEINNEADKQIRTPAGDKLLNTLSS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHCCCCHHHHHHHHCC
NGIFVSSACGGGGSCGQCRVTVKEGGGDILPTELSHITKRDAKAGCRLACQVAVKQNMKI
CCEEEEECCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEEHHCCCCE
ELPEEIFGVKKWECEVISNDNKATFIKELKLRIPEGEVVPFRAGGYIQIECPPHKVAYAD
ECCHHHHCCCCEEEEEECCCCCHHEEEHHHHCCCCCCEEEEECCCEEEEECCCCEEEEEC
FDVPDEYRSDWDKFNLFRYVSEVKEPTLRAYSMANYPEEKGIIMLNVRIATPPPKVPDAP
CCCCHHHHCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCC
PGIMSSYIWSLKPGDKVTISGPFGEFFAKETDAEMVFIGGGAGMAPMRSHIFDQLKRLHS
CCHHHHHHEECCCCCEEEEECCCHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHC
TRKISFWYGARSLREMFYDEEFEQLARDNPNFTFHVALSDPLPEDNWTGHTGFIHNVLYE
CCEEEEECCHHHHHHHHCCHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHH
NYLRDHPAPEDCEFYMCGPPVMNAAVIKMLKDLGVEDENILLDDFGG
HHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCEEEECCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dihydropteridine; 2 nitric oxide; O2; NAD(P)H [C]

Specific reaction: NAD(P)H + dihydropteridine = NAD(P)+ @TETR01.txt*tetrahydropteridine! 2 nitric oxide + 2 O2 + NAD(P)H = 2 NO3- + NAD(P) + Proton [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA