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The map label for this gene is proS [H]

Identifier: 238893191

GI number: 238893191

Start: 1029448

End: 1031166

Strand: Reverse

Name: proS [H]

Synonym: KP1_1053

Alternate gene names: 238893191

Gene position: 1031166-1029448 (Counterclockwise)

Preceding gene: 238893192

Following gene: 238893190

Centisome position: 19.65

GC content: 58.12

Gene sequence:

>1719_bases
ATGCGTACTAGCCAATATCTGCTCTCCACTCTGAAGGAGACACCTGCCGACGCCGAGGTAATCAGCCATCAGCTGATGCT
GCGCGCCGGGATGATCCGCAAGCTGGCCTCCGGGTTATACACCTGGCTGCCGACCGGCGTGCGCGTCCTGAAAAAAGTCG
AAAACATCGTGCGTGAAGAAATGAACAACGCCGGTGCGATCGAGGTGTTAATGCCGGTCGTTCAGCCATCTGAACTGTGG
CAGGAGAGCGGGCGCTGGGAGCAGTACGGCCCGGAACTGTTGCGCATTGCCGATCGCGGCGATCGTCCGTTCGTCCTCGG
CCCAACCCACGAAGAGGTCATTACCGATCTGATCCGCAACGAGCTGAACTCCTATAAACAGCTGCCGCTGAACTTCTATC
AGATCCAGACCAAATTCCGCGACGAAGTACGCCCGCGTTTCGGGGTGATGCGCTCCCGTGAATTCCTGATGAAAGATGCC
TACTCTTTCCATACTTCGCAGGAATCGCTGCAGGAAACCTACGACGCGATGTATGCCGCTTACAGCAAAATCTTCAGCCG
TATGGGGCTGGATTTCCGCGCGGTACAGGCTGACACCGGTTCTATCGGCGGCAGCGCCTCTCATGAATTCCAGGTGCTGG
CGCAGAGCGGCGAAGACGATGTGATCTTCTCCGACAGCTCCGACTACGCGGCAAACATCGAGTTTGCGGAAGCCGTTGCG
CCGAAAGAGCCGCGCGCAGCCGCCACCCAGGAGATGACCCTGGTCGATACGCCGAACGCCAAAACCATCGCTGAGCTGGT
GGAGCAGTTCAACCTGCCGATCGAAAAAACCGTGAAAACTCTGCTGGTGAAAGCGGTTGAAGATAGCGCTTCCCCGCTGG
TTGCCCTGCTGGTGCGCGGCGATCACGAGCTGAACGAAGTGAAAGCGGAAAAACTGCCGCAGGTCGCCAGCCCGCTGACC
TTCGCCACCGAAGAAGAGATCCGCGCCCTGGTGAACGCCGGTCCGGGCTCCCTGGGCCCGGTGAACATGCCGGTTCCGGT
GATTATCGACCGTACTGTAGCGGTGATGAGCGATTTCGCCGCTGGCGCCAATATCGATGGCAAACACTACTTCGGCATCA
ACTGGGACCGCGATGTCGCGACGCCGGAAGTGGCCGACATCCGTAACGTCGTCGCCGGCGACCCGAGCCCGGACGGTAAA
GGTACCCTGCTTATTAAGCGCGGTATCGAAGTGGGTCATATCTTCCAGCTGGGGACGAAGTACTCCGAGGCGATGAAAGC
CGCGGTCCAGGGCGAAGATGGGCGTAACCAGATCCTGACCATGGGCTGCTATGGTATCGGGGTGACTCGCGTGGTAGCGG
CGGCGATTGAGCAGAACTTTGACGATCGCGGTATCGTCTGGCCGGATGCGATTGCGCCTTTCCAGGTGGCGATCCTGCCG
ATGAACATGCACAAGTCTTACCGTGTGCAGGAGCTGGCGGAGAAACTGTACACCGAGCTGAGCGCCCAGGGTATTGAAGT
GCTGATGGACGATCGTAAAGAGCGTCCGGGCGTGATGTTCGCCGATATGGAGCTGATCGGTATCCCGCACACCATCGTGC
TGGGCGATCGTAACCTCGACAACGATGATATCGAATACAAGTATCGTCGCAACGGTGAGAAGCAGCTGATTAAGACCGGG
GATATTGTGGAATATCTGGTTAAAGCGATAAAAGGCTAA

Upstream 100 bases:

>100_bases
CTCTTTTGGCATCTTTGCCACACTGGTAAACTAAACCACTTTTTTTGTTTCAGGCTGGGCAACCAGCCTGTTCCGCTCGT
TTAACTGGAACCGTTACAAC

Downstream 100 bases:

>100_bases
CACCCACCTCAGGGCCCTGAACGGATGTCAGGGCCTTTTTTTTTACAAGGACAGTACCAATGACTGCACATCTCCTCCGC
AGCGGACTGCTTACTTTTGC

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLPTGVRVLKKVENIVREEMNNAGAIEVLMPVVQPSELW
QESGRWEQYGPELLRIADRGDRPFVLGPTHEEVITDLIRNELNSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGEDDVIFSDSSDYAANIEFAEAVA
PKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEKTVKTLLVKAVEDSASPLVALLVRGDHELNEVKAEKLPQVASPLT
FATEEEIRALVNAGPGSLGPVNMPVPVIIDRTVAVMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVAGDPSPDGK
GTLLIKRGIEVGHIFQLGTKYSEAMKAAVQGEDGRNQILTMGCYGIGVTRVVAAAIEQNFDDRGIVWPDAIAPFQVAILP
MNMHKSYRVQELAEKLYTELSAQGIEVLMDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTG
DIVEYLVKAIKG

Sequences:

>Translated_572_residues
MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLPTGVRVLKKVENIVREEMNNAGAIEVLMPVVQPSELW
QESGRWEQYGPELLRIADRGDRPFVLGPTHEEVITDLIRNELNSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGEDDVIFSDSSDYAANIEFAEAVA
PKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEKTVKTLLVKAVEDSASPLVALLVRGDHELNEVKAEKLPQVASPLT
FATEEEIRALVNAGPGSLGPVNMPVPVIIDRTVAVMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVAGDPSPDGK
GTLLIKRGIEVGHIFQLGTKYSEAMKAAVQGEDGRNQILTMGCYGIGVTRVVAAAIEQNFDDRGIVWPDAIAPFQVAILP
MNMHKSYRVQELAEKLYTELSAQGIEVLMDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTG
DIVEYLVKAIKG
>Mature_572_residues
MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLPTGVRVLKKVENIVREEMNNAGAIEVLMPVVQPSELW
QESGRWEQYGPELLRIADRGDRPFVLGPTHEEVITDLIRNELNSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGEDDVIFSDSSDYAANIEFAEAVA
PKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEKTVKTLLVKAVEDSASPLVALLVRGDHELNEVKAEKLPQVASPLT
FATEEEIRALVNAGPGSLGPVNMPVPVIIDRTVAVMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVAGDPSPDGK
GTLLIKRGIEVGHIFQLGTKYSEAMKAAVQGEDGRNQILTMGCYGIGVTRVVAAAIEQNFDDRGIVWPDAIAPFQVAILP
MNMHKSYRVQELAEKLYTELSAQGIEVLMDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTG
DIVEYLVKAIKG

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=226, Percent_Identity=45.575221238938, Blast_Score=218, Evalue=2e-56,
Organism=Escherichia coli, GI1786392, Length=572, Percent_Identity=94.5804195804196, Blast_Score=1125, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115532348, Length=261, Percent_Identity=38.3141762452107, Blast_Score=176, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI193203271, Length=82, Percent_Identity=39.0243902439024, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71984192, Length=167, Percent_Identity=25.1497005988024, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71984184, Length=167, Percent_Identity=25.1497005988024, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320931, Length=548, Percent_Identity=30.2919708029197, Blast_Score=250, Evalue=3e-67,
Organism=Drosophila melanogaster, GI24656200, Length=220, Percent_Identity=38.1818181818182, Blast_Score=180, Evalue=2e-45,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 63554; Mature: 63554

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLPTGVRVLKKVENIVREE
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNNAGAIEVLMPVVQPSELWQESGRWEQYGPELLRIADRGDRPFVLGPTHEEVITDLIRN
HCCCCHHHHHHHHCCHHHHHHHCCCCHHHCHHHHHHHCCCCCCEEECCCHHHHHHHHHHH
ELNSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDAYSFHTSQESLQETYDAMYAA
HHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
YSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGEDDVIFSDSSDYAANIEFAEAVA
HHHHHHHHCCCEEEEECCCCCCCCCCCCHHHEEECCCCCCEEEECCCCCEECCCHHHHCC
PKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEKTVKTLLVKAVEDSASPLVALLVRG
CCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEEECC
DHELNEVKAEKLPQVASPLTFATEEEIRALVNAGPGSLGPVNMPVPVIIDRTVAVMSDFA
CCHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEHHHHHHHHHHH
AGANIDGKHYFGINWDRDVATPEVADIRNVVAGDPSPDGKGTLLIKRGIEVGHIFQLGTK
CCCCCCCCEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHH
YSEAMKAAVQGEDGRNQILTMGCYGIGVTRVVAAAIEQNFDDRGIVWPDAIAPFQVAILP
HHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEE
MNMHKSYRVQELAEKLYTELSAQGIEVLMDDRKERPGVMFADMELIGIPHTIVLGDRNLD
ECCCCCHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCEEEECEEEECCCEEEEECCCCCC
NDDIEYKYRRNGEKQLIKTGDIVEYLVKAIKG
CCCCCCHHHCCCCHHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure
MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLPTGVRVLKKVENIVREE
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNNAGAIEVLMPVVQPSELWQESGRWEQYGPELLRIADRGDRPFVLGPTHEEVITDLIRN
HCCCCHHHHHHHHCCHHHHHHHCCCCHHHCHHHHHHHCCCCCCEEECCCHHHHHHHHHHH
ELNSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDAYSFHTSQESLQETYDAMYAA
HHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
YSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGEDDVIFSDSSDYAANIEFAEAVA
HHHHHHHHCCCEEEEECCCCCCCCCCCCHHHEEECCCCCCEEEECCCCCEECCCHHHHCC
PKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEKTVKTLLVKAVEDSASPLVALLVRG
CCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEEECC
DHELNEVKAEKLPQVASPLTFATEEEIRALVNAGPGSLGPVNMPVPVIIDRTVAVMSDFA
CCHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEHHHHHHHHHHH
AGANIDGKHYFGINWDRDVATPEVADIRNVVAGDPSPDGKGTLLIKRGIEVGHIFQLGTK
CCCCCCCCEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHH
YSEAMKAAVQGEDGRNQILTMGCYGIGVTRVVAAAIEQNFDDRGIVWPDAIAPFQVAILP
HHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEE
MNMHKSYRVQELAEKLYTELSAQGIEVLMDDRKERPGVMFADMELIGIPHTIVLGDRNLD
ECCCCCHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCEEEECEEEECCCEEEEECCCCCC
NDDIEYKYRRNGEKQLIKTGDIVEYLVKAIKG
CCCCCCHHHCCCCHHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA