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The map label for this gene is murD [H]

Identifier: 238893068

GI number: 238893068

Start: 894214

End: 895530

Strand: Direct

Name: murD [H]

Synonym: KP1_0910

Alternate gene names: 238893068

Gene position: 894214-895530 (Clockwise)

Preceding gene: 238893067

Following gene: 238893069

Centisome position: 17.04

GC content: 61.2

Gene sequence:

>1317_bases
ATGGCAGATTATCAGGGTAAAAAAGTCGTCATTATTGGGCTGGGCATGACCGGCCTGTCATGCGTGGACTTTTTCATGGC
GCGCGGCGTAACCCCGCGCGTGATGGACACCCGCGTCGCGCCTCCGGGGCTGGATAAATTACCCGAATCGGTCGAATGCC
ATGTCGGCGGACTCAATGATACCTGGCTGCTGGCGGCGGACCTGATTGTCGCCAGTCCGGGGATTGCGCTGGCCCATCCT
TCGCTCAGCGCAGCGGCCGACGCTGGCGTAGAGATCGTTGGCGATATCGAACTGTTCTGCCGCGAAGCGCAGGCGCCGAT
TATCGCCATTACCGGTTCTAACGGCAAAAGCACTGTTACTACCCTGGTGGGCGAGATGGCGAAAGCCGCTGGAGTTAACG
TCGGCGTGGGCGGCAATATCGGCCTGCCGGCGCTGATGCTGCTGGACACCGATCGAGAGCTGTATGTGTTGGAGCTCTCC
AGCTTCCAGCTGGAAACCACCTTCAGCCTGCAGGCGGTGGCGGCAACCATCCTCAACGTGACTGAGGACCATATGGACCG
CTACCCGCTGGGGCTGCAGCAGTATCGCGCGGCGAAGCTGCGGATTTACGAGAATGCGAAGACCTGCGTGGTGAATGCGG
ACGATGCGCTCACCATGCCGGTACGCGGGGCGGACGAACGCTGCATTAGCTTTGGCGTCGATGTCGGTGACTACCACCTT
AACCGCCAGCAGGGCGAAACCTGGCTGCGCGTTAAAGGTGAGAAAGTCCTCAATGTGAAAGAGATGCCTCTCACCGGTCA
GCATAACTACAGCAACGCCCTGGCGGCGCTGGCGCTGGCCGATGCCGCAGGTCTGCCGCGGGCCAGCAGCCTGAAGGCGC
TGACCACCTTTACCGGTCTGGCGCACCGTTTCCAGTTGGTGCAGGAACACAACGGCGTACGCTGGATTAACGACTCGAAG
GCGACCAACGTCGGCAGCACTGAGGCGGCGCTGAACGGCCTGCAGCTGGACGGCACGCTCTATCTGCTGCTGGGCGGCGA
CGGCAAGTCCGCTGATTTCACCCCGCTGAAGCGCTACCTCGGCGGCGATCGTATTCGCCTGTACTGCTTTGGTCGCGATG
GCGCCGAGCTCGCTGAGCTGCGTCCTGAGGTGGCGGTACAAACTGAGACTATGGAACAGGCGATGCGTCAGATTGCTCCC
CTGGTGAAAGCGGGGGACATGGTGCTCCTGTCGCCGGCCTGCGCCAGCCTGGATCAGTTTAAGAACTTTGAACAACGGGG
CGAGATGTTTGCCCGTCTGGCGAAGGAGTTAGGTTGA

Upstream 100 bases:

>100_bases
AACTGAAAGGCTGGCCGGAGCCGCGCGTTATCGTGCGCTTCTGGATTATTTCGCTGATGCTGGTGCTGATTGGCCTGGCG
ACGCTGAAGGTACGTTAATC

Downstream 100 bases:

>100_bases
TGCGTTTCTCTCTCCCACGCCTGAGAATGCCGCAGCTGAGCCTGCCTCGGCTGAGACTGCCGCGACTGCCGGGGATGTTT
ATTTTCGCCTGGCTATTCGC

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MADYQGKKVVIIGLGMTGLSCVDFFMARGVTPRVMDTRVAPPGLDKLPESVECHVGGLNDTWLLAADLIVASPGIALAHP
SLSAAADAGVEIVGDIELFCREAQAPIIAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDTDRELYVLELS
SFQLETTFSLQAVAATILNVTEDHMDRYPLGLQQYRAAKLRIYENAKTCVVNADDALTMPVRGADERCISFGVDVGDYHL
NRQQGETWLRVKGEKVLNVKEMPLTGQHNYSNALAALALADAAGLPRASSLKALTTFTGLAHRFQLVQEHNGVRWINDSK
ATNVGSTEAALNGLQLDGTLYLLLGGDGKSADFTPLKRYLGGDRIRLYCFGRDGAELAELRPEVAVQTETMEQAMRQIAP
LVKAGDMVLLSPACASLDQFKNFEQRGEMFARLAKELG

Sequences:

>Translated_438_residues
MADYQGKKVVIIGLGMTGLSCVDFFMARGVTPRVMDTRVAPPGLDKLPESVECHVGGLNDTWLLAADLIVASPGIALAHP
SLSAAADAGVEIVGDIELFCREAQAPIIAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDTDRELYVLELS
SFQLETTFSLQAVAATILNVTEDHMDRYPLGLQQYRAAKLRIYENAKTCVVNADDALTMPVRGADERCISFGVDVGDYHL
NRQQGETWLRVKGEKVLNVKEMPLTGQHNYSNALAALALADAAGLPRASSLKALTTFTGLAHRFQLVQEHNGVRWINDSK
ATNVGSTEAALNGLQLDGTLYLLLGGDGKSADFTPLKRYLGGDRIRLYCFGRDGAELAELRPEVAVQTETMEQAMRQIAP
LVKAGDMVLLSPACASLDQFKNFEQRGEMFARLAKELG
>Mature_437_residues
ADYQGKKVVIIGLGMTGLSCVDFFMARGVTPRVMDTRVAPPGLDKLPESVECHVGGLNDTWLLAADLIVASPGIALAHPS
LSAAADAGVEIVGDIELFCREAQAPIIAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDTDRELYVLELSS
FQLETTFSLQAVAATILNVTEDHMDRYPLGLQQYRAAKLRIYENAKTCVVNADDALTMPVRGADERCISFGVDVGDYHLN
RQQGETWLRVKGEKVLNVKEMPLTGQHNYSNALAALALADAAGLPRASSLKALTTFTGLAHRFQLVQEHNGVRWINDSKA
TNVGSTEAALNGLQLDGTLYLLLGGDGKSADFTPLKRYLGGDRIRLYCFGRDGAELAELRPEVAVQTETMEQAMRQIAPL
VKAGDMVLLSPACASLDQFKNFEQRGEMFARLAKELG

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=438, Percent_Identity=88.8127853881279, Blast_Score=779, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 46952; Mature: 46821

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADYQGKKVVIIGLGMTGLSCVDFFMARGVTPRVMDTRVAPPGLDKLPESVECHVGGLND
CCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCHHHHCCCCCCCHHHCCCCCEEEECCCCC
TWLLAADLIVASPGIALAHPSLSAAADAGVEIVGDIELFCREAQAPIIAITGSNGKSTVT
HHHHHHHHHHCCCCCEEECCCCHHHHCCCCEEEECHHHHHHHCCCCEEEEECCCCCHHHH
TLVGEMAKAAGVNVGVGGNIGLPALMLLDTDRELYVLELSSFQLETTFSLQAVAATILNV
HHHHHHHHHCCCEECCCCCCCCCEEEEEECCCCEEEEEECCEEEEEEHHHHHHHHHHHHH
TEDHMDRYPLGLQQYRAAKLRIYENAKTCVVNADDALTMPVRGADERCISFGVDVGDYHL
HHHHHHCCCCCHHHHHHEEEEEEECCCEEEEECCCCEEECCCCCCCHHHEECCCCCCEEC
NRQQGETWLRVKGEKVLNVKEMPLTGQHNYSNALAALALADAAGLPRASSLKALTTFTGL
CHHCCCEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
AHRFQLVQEHNGVRWINDSKATNVGSTEAALNGLQLDGTLYLLLGGDGKSADFTPLKRYL
HHHHHHHHHCCCEEEECCCCCCCCCCHHHHHCCEEECCEEEEEECCCCCCCCCHHHHHHC
GGDRIRLYCFGRDGAELAELRPEVAVQTETMEQAMRQIAPLVKAGDMVLLSPACASLDQF
CCCEEEEEEECCCCHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHH
KNFEQRGEMFARLAKELG
HCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ADYQGKKVVIIGLGMTGLSCVDFFMARGVTPRVMDTRVAPPGLDKLPESVECHVGGLND
CCCCCCEEEEEEECCCHHHHHHHHHHCCCCCHHHHCCCCCCCHHHCCCCCEEEECCCCC
TWLLAADLIVASPGIALAHPSLSAAADAGVEIVGDIELFCREAQAPIIAITGSNGKSTVT
HHHHHHHHHHCCCCCEEECCCCHHHHCCCCEEEECHHHHHHHCCCCEEEEECCCCCHHHH
TLVGEMAKAAGVNVGVGGNIGLPALMLLDTDRELYVLELSSFQLETTFSLQAVAATILNV
HHHHHHHHHCCCEECCCCCCCCCEEEEEECCCCEEEEEECCEEEEEEHHHHHHHHHHHHH
TEDHMDRYPLGLQQYRAAKLRIYENAKTCVVNADDALTMPVRGADERCISFGVDVGDYHL
HHHHHHCCCCCHHHHHHEEEEEEECCCEEEEECCCCEEECCCCCCCHHHEECCCCCCEEC
NRQQGETWLRVKGEKVLNVKEMPLTGQHNYSNALAALALADAAGLPRASSLKALTTFTGL
CHHCCCEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
AHRFQLVQEHNGVRWINDSKATNVGSTEAALNGLQLDGTLYLLLGGDGKSADFTPLKRYL
HHHHHHHHHCCCEEEECCCCCCCCCCHHHHHCCEEECCEEEEEECCCCCCCCCHHHHHHC
GGDRIRLYCFGRDGAELAELRPEVAVQTETMEQAMRQIAPLVKAGDMVLLSPACASLDQF
CCCEEEEEEECCCCHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHH
KNFEQRGEMFARLAKELG
HCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]