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The map label for this gene is fruR [H]

Identifier: 238893060

GI number: 238893060

Start: 885115

End: 886119

Strand: Direct

Name: fruR [H]

Synonym: KP1_0900

Alternate gene names: 238893060

Gene position: 885115-886119 (Clockwise)

Preceding gene: 238893059

Following gene: 238893061

Centisome position: 16.86

GC content: 59.1

Gene sequence:

>1005_bases
GTGAAACTGGATGAAATCGCCCGGCTGGCCGGTGTTTCAAGAACCACCGCGAGCTATGTGATTAATGGCAAAGCGAAGCA
GTACCGCGTAAGCGATAAAACGGTCGAAAAAGTGATGGCTGTGGTGCGAGAGCACAACTATCACCCAAATGCGGTCGCTG
CTGGCCTGCGTGCAGGACGCACACGCTCTATCGGTCTGGTGATCCCGGATCTGGAAAACACCAGCTATACCCGGATTGCA
AATTATCTCGAACGTCAGGCGCGTCAGCGCGGCTATCAGCTGCTGATTGCCTGCTCGGAGGATCAGCCCGATAACGAGAT
GCGCTGTATCGAGCATCTGCTGCAGCGCCAGGTGGACGCTATCATTGTCTCTACTTCGCTGCCGCCGGAGCACCCTTTTT
ATCAGCGCTGGGCTAACGACCCGTTTCCGATTGTCGCCCTCGACCGCGCCCTCGATCGCGAGCACTTCACCAGCGTGGTT
GGCGCTGACCAGGATGACGCCGAGATGCTCGCAGCTGAGCTGCGGACGTTTCCTGGCGAAACGGTGCTCTATCTCGGCGC
GCTGCCGGAGCTGTCGGTGAGCTTCCTGCGCGAACAGGGCTTCCGTAAAGCCTGGCAGGACGATCCGCGTGAGGTGCATT
ATCTCTATGCCAACAGCTATGAGCGCGAAGCGGCGGCCCAGCTGTTTGAGAAATGGCTTGAAACCCATCCGATGCCACAG
GCGCTGTTTACCACCTCTTTCCCGCTGCTGCAGGGCGTAATGGATGTGACCTTGCGTCGCGAGGGTAAACTGCCCTCCGA
GCTGGCGATCGCCACCTTCGGTGATAATGAGCTGCTCGATTTTCTGCAGTGTCCGGTACTGGCCGTGGCCCAGCGTCATC
GCGATGTCGCTGAGCGGGTGCTGGAGATCGTCCTCGCCAGCCTCGACGAACCTCGCAAGCCGAAGCCGGGTCTTAGCCGT
ATTCGCCGTAATCTCTATCGTCGCGGTAGCCTGAATCGCCGCTAA

Upstream 100 bases:

>100_bases
AAGCGGTTGCTGCGCTAACTATTCTGCGCTTAGATGTTATAAAAAGGATTCCATACCTGAATAATGGTTATGTTTGGCAC
TTCTTACCCAAGGGGCAATT

Downstream 100 bases:

>100_bases
GGGCGCCATATCGCCAGCGCCCAGGTATAAACCTGGCAGGCCTGACCGTAAGCAGGCCGCTGGCGATAAATCAGGCAATT
GACGTGCAAAATGCAAAACT

Product: DNA-binding transcriptional regulator FruR

Products: NA

Alternate protein names: Catabolite repressor/activator [H]

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVREHNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIA
NYLERQARQRGYQLLIACSEDQPDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
GADQDDAEMLAAELRTFPGETVLYLGALPELSVSFLREQGFRKAWQDDPREVHYLYANSYEREAAAQLFEKWLETHPMPQ
ALFTTSFPLLQGVMDVTLRREGKLPSELAIATFGDNELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLSR
IRRNLYRRGSLNRR

Sequences:

>Translated_334_residues
MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVREHNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIA
NYLERQARQRGYQLLIACSEDQPDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
GADQDDAEMLAAELRTFPGETVLYLGALPELSVSFLREQGFRKAWQDDPREVHYLYANSYEREAAAQLFEKWLETHPMPQ
ALFTTSFPLLQGVMDVTLRREGKLPSELAIATFGDNELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLSR
IRRNLYRRGSLNRR
>Mature_334_residues
MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVREHNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIA
NYLERQARQRGYQLLIACSEDQPDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
GADQDDAEMLAAELRTFPGETVLYLGALPELSVSFLREQGFRKAWQDDPREVHYLYANSYEREAAAQLFEKWLETHPMPQ
ALFTTSFPLLQGVMDVTLRREGKLPSELAIATFGDNELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLSR
IRRNLYRRGSLNRR

Specific function: Represses some operons but activates others. It is implicated in the regulation of a large number of operons encoding enzymes which comprise central pathways of carbon metabolism. Binds D-fructose as an inducer [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786268, Length=333, Percent_Identity=96.0960960960961, Blast_Score=662, Evalue=0.0,
Organism=Escherichia coli, GI1790194, Length=320, Percent_Identity=27.5, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1787580, Length=289, Percent_Identity=23.5294117647059, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787948, Length=308, Percent_Identity=22.4025974025974, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1789068, Length=304, Percent_Identity=25, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1790369, Length=309, Percent_Identity=23.9482200647249, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790715, Length=284, Percent_Identity=25.7042253521127, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1789202, Length=128, Percent_Identity=32.03125, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788474, Length=207, Percent_Identity=26.0869565217391, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012781
- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 38096; Mature: 38096

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVREHNYHPNAVAAGLRAGR
CCHHHHHHHHCCCHHHHHHEECCCCCEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHCCC
TRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQPDNEMRCIEHLLQRQVDA
CCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHE
IIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVVGADQDDAEMLAAELRTFPGE
EEEECCCCCCCCHHHHHCCCCCCEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
TVLYLGALPELSVSFLREQGFRKAWQDDPREVHYLYANSYEREAAAQLFEKWLETHPMPQ
EEEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCH
ALFTTSFPLLQGVMDVTLRREGKLPSELAIATFGDNELLDFLQCPVLAVAQRHRDVAERV
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
LEIVLASLDEPRKPKPGLSRIRRNLYRRGSLNRR
HHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVREHNYHPNAVAAGLRAGR
CCHHHHHHHHCCCHHHHHHEECCCCCEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHCCC
TRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQPDNEMRCIEHLLQRQVDA
CCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHE
IIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVVGADQDDAEMLAAELRTFPGE
EEEECCCCCCCCHHHHHCCCCCCEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
TVLYLGALPELSVSFLREQGFRKAWQDDPREVHYLYANSYEREAAAQLFEKWLETHPMPQ
EEEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCH
ALFTTSFPLLQGVMDVTLRREGKLPSELAIATFGDNELLDFLQCPVLAVAQRHRDVAERV
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
LEIVLASLDEPRKPKPGLSRIRRNLYRRGSLNRR
HHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]