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The map label for this gene is proP [H]

Identifier: 238892997

GI number: 238892997

Start: 807602

End: 808906

Strand: Direct

Name: proP [H]

Synonym: KP1_0830

Alternate gene names: 238892997

Gene position: 807602-808906 (Clockwise)

Preceding gene: 238892996

Following gene: 238893001

Centisome position: 15.39

GC content: 61.92

Gene sequence:

>1305_bases
ATGTCATATGCAAACCGACCGCTCAACCGCCAGGATTATAAAACGCTGACGCTGGCCGCCCTGGGCGGCGCGCTGGAGTT
TTATGACTTTATCATTTTCGTTTTCTTCGCCGCCGTCGTCGGCGAGCTCTTCTTCCCTGCGGATATTCCCGAATGGCTGC
GCCAGGTGCAAACCTTTGGCATTTTCGCCGCCGGTTACCTGGCGCGTCCGCTGGGGGGCATCATCATGGCCCACTTTGGC
GATCTGGTGGGGCGCAAGAAAATGTTCACCCTCAGCATCCTGCTGATGGCGCTGCCAACCCTGGCGATCGGTCTGCTACC
GACCTATAGCTCGGTAGGTATCATCGCCCCGCTGCTATTGCTGCTGATGCGTATTCTGCAGGGAGCGGCGATCGGCGGTG
AAGTTCCAGGCGCCTGGGTGTTTGTCGCTGAGCATGTCCCGGAAAAACGCATTGGTATCGCCTGCGGCACGCTGACGGCC
GGCCTGACGGTTGGCATTCTGCTGGGGTCGGTGGTGGCCACGCTTATCAATACCAGTCTGACGCCGCAGGGGATCCACGA
CGGCGGCTGGCGGATCCCCTTCCTGCTGGGCGGCGTATTTGGTCTGATAGCGATGTATCTGCGCCGCTGGCTGCAGGAGA
CGCCGATTTTTCTTGAGATGCAGCAGCGCAAAGCGCTGGCGCAGGAGCTGCCGGTAAAAGCGGTGGCGCTCAAGCACCAG
AAGGCGGTGGTGATCTCTATGCTGCTGACCTGGCTGCTGTCGGCTGGCGTGGTGGTGGTGATCCTGATGTCGCCGGTCTG
GCTGCAGAAGCACTATGGTTTTGCGCCGGCCATTACCCTGCAGGCCAACAGCATCGCCACCATTATGTTGTGCATCGGCT
GTCTGCTCGCCGGGCTGGCAGCGGACCGCTTCGGCGCCAGCCGCACTTTTATCGTCGGCAGCCTGCTGCTGGCGGTTGCC
AGCTGGGCATTTTACCATCTTGCCGGCGTCAGCCCGCAGCGGCTGTTTCTGCTCTACGGTACGGTGGGGCTTTGCGTCGG
CGTGGTGGGGGCGGTGCCCTATGTGATGGTGCGGGCCTTCCCGGCGGAAGTACGCTTCACCGGCATCTCTTTCTCCTATA
ACCTGTCGTACGCGATCTTTGGCGGCCTGACGCCGATTGCGGTCACCATGCTGATGGGGGTGTCGCCGATGGCGCCGGCC
TGGTATGTGCTGGCCCTGTCGCTGATGGGGCTGGGATTAGGTATCTGGCTGCGTCAGGAGCTGACGGCGCCGACGGGGAT
GCCGGAAGGGGAGTTGCAGCGTTAA

Upstream 100 bases:

>100_bases
TAACCTGCATCGCTAATGGTGTGCCGTTTTATTTACGATATAGTGATTTTTACTTTACACACTATAAAACACGCTGCACA
CCTCAGGATGAAATTGCGTT

Downstream 100 bases:

>100_bases
CGATGGCTAGCTGATGCTGATAATGACCTGCCGCCGCCCTGTGACCGGACGGCGGCGGAAGATTAGCGCGGTTCGCCGAT
CGGCAGCACGGTGCGGCCGA

Product: putative major facilitator superfamily proline/betaine transport system permease component

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MSYANRPLNRQDYKTLTLAALGGALEFYDFIIFVFFAAVVGELFFPADIPEWLRQVQTFGIFAAGYLARPLGGIIMAHFG
DLVGRKKMFTLSILLMALPTLAIGLLPTYSSVGIIAPLLLLLMRILQGAAIGGEVPGAWVFVAEHVPEKRIGIACGTLTA
GLTVGILLGSVVATLINTSLTPQGIHDGGWRIPFLLGGVFGLIAMYLRRWLQETPIFLEMQQRKALAQELPVKAVALKHQ
KAVVISMLLTWLLSAGVVVVILMSPVWLQKHYGFAPAITLQANSIATIMLCIGCLLAGLAADRFGASRTFIVGSLLLAVA
SWAFYHLAGVSPQRLFLLYGTVGLCVGVVGAVPYVMVRAFPAEVRFTGISFSYNLSYAIFGGLTPIAVTMLMGVSPMAPA
WYVLALSLMGLGLGIWLRQELTAPTGMPEGELQR

Sequences:

>Translated_434_residues
MSYANRPLNRQDYKTLTLAALGGALEFYDFIIFVFFAAVVGELFFPADIPEWLRQVQTFGIFAAGYLARPLGGIIMAHFG
DLVGRKKMFTLSILLMALPTLAIGLLPTYSSVGIIAPLLLLLMRILQGAAIGGEVPGAWVFVAEHVPEKRIGIACGTLTA
GLTVGILLGSVVATLINTSLTPQGIHDGGWRIPFLLGGVFGLIAMYLRRWLQETPIFLEMQQRKALAQELPVKAVALKHQ
KAVVISMLLTWLLSAGVVVVILMSPVWLQKHYGFAPAITLQANSIATIMLCIGCLLAGLAADRFGASRTFIVGSLLLAVA
SWAFYHLAGVSPQRLFLLYGTVGLCVGVVGAVPYVMVRAFPAEVRFTGISFSYNLSYAIFGGLTPIAVTMLMGVSPMAPA
WYVLALSLMGLGLGIWLRQELTAPTGMPEGELQR
>Mature_433_residues
SYANRPLNRQDYKTLTLAALGGALEFYDFIIFVFFAAVVGELFFPADIPEWLRQVQTFGIFAAGYLARPLGGIIMAHFGD
LVGRKKMFTLSILLMALPTLAIGLLPTYSSVGIIAPLLLLLMRILQGAAIGGEVPGAWVFVAEHVPEKRIGIACGTLTAG
LTVGILLGSVVATLINTSLTPQGIHDGGWRIPFLLGGVFGLIAMYLRRWLQETPIFLEMQQRKALAQELPVKAVALKHQK
AVVISMLLTWLLSAGVVVVILMSPVWLQKHYGFAPAITLQANSIATIMLCIGCLLAGLAADRFGASRTFIVGSLLLAVAS
WAFYHLAGVSPQRLFLLYGTVGLCVGVVGAVPYVMVRAFPAEVRFTGISFSYNLSYAIFGGLTPIAVTMLMGVSPMAPAW
YVLALSLMGLGLGIWLRQELTAPTGMPEGELQR

Specific function: May be a proton symporter invoved in the uptake of osmolytes such as proline and glycine betaine [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=429, Percent_Identity=30.3030303030303, Blast_Score=194, Evalue=8e-51,
Organism=Escherichia coli, GI1788292, Length=420, Percent_Identity=30, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI1789941, Length=381, Percent_Identity=32.2834645669291, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1788942, Length=431, Percent_Identity=29.0023201856148, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI87082231, Length=434, Percent_Identity=22.5806451612903, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI87082404, Length=408, Percent_Identity=22.0588235294118, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 46701; Mature: 46569

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HCCCCCCCCCCCCC
>Mature Secondary Structure 
SYANRPLNRQDYKTLTLAALGGALEFYDFIIFVFFAAVVGELFFPADIPEWLRQVQTFG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
IFAAGYLARPLGGIIMAHFGDLVGRKKMFTLSILLMALPTLAIGLLPTYSSVGIIAPLLL
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LLMRILQGAAIGGEVPGAWVFVAEHVPEKRIGIACGTLTAGLTVGILLGSVVATLINTSL
HHHHHHCCCCCCCCCCCCEEEEEHHCCCHHHCEEHHHHHHHHHHHHHHHHHHHHHHHCCC
TPQGIHDGGWRIPFLLGGVFGLIAMYLRRWLQETPIFLEMQQRKALAQELPVKAVALKHQ
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHEEHHHHHHHHHHCCHHHHHHHHH
KAVVISMLLTWLLSAGVVVVILMSPVWLQKHYGFAPAITLQANSIATIMLCIGCLLAGLA
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHH
ADRFGASRTFIVGSLLLAVASWAFYHLAGVSPQRLFLLYGTVGLCVGVVGAVPYVMVRAF
HHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PAEVRFTGISFSYNLSYAIFGGLTPIAVTMLMGVSPMAPAWYVLALSLMGLGLGIWLRQE
CCCEEEEEEEEEECCCHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LTAPTGMPEGELQR
HCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA