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The map label for this gene is dcuB [H]

Identifier: 238892459

GI number: 238892459

Start: 235977

End: 237317

Strand: Direct

Name: dcuB [H]

Synonym: KP1_0224

Alternate gene names: 238892459

Gene position: 235977-237317 (Clockwise)

Preceding gene: 238892458

Following gene: 238892471

Centisome position: 4.5

GC content: 55.56

Gene sequence:

>1341_bases
ATGGAATTCGCGATACAACTCATTATTATATTAATTTGTCTGTTTTATGGTGCCCGAAAAGGGGGGATTGCGCTGGGGCT
GCTGGGCGGCATCGGTTTGGTGATCCTCGTCTTTGTTTTTCATCTCCAGCCGGGCAAACCGCCGGTTGATGTGATGCTGG
TGATCATTGCAGTGGTAGCCGCCTCGGCGACCCTGCAGGCGTCCGGCGGGTTAGACGTGATGCTGCAGATTGCCGAGAAG
CTGCTGCGGCGTAACCCGAAATATGTTTCTATCGTGGCGCCTTTCGTCACCTGCACGCTGACCATCCTCTGCGGTACCGG
CCACGTGGTGTATACCATCCTGCCGATTATCTACGATGTGGCGATTAAGAATAACATTCGTCCGGAGCGTCCGATGGCCG
CCAGCTCCATTGGCGCTCAGATGGGGATCATCGCCAGCCCGGTCTCGGTGGCGGTGGTATCGCTGGTGGCGATGCTGGGA
AATTTTACCTTTAACGGGAAACACCTGGAGTTTCTCGATCTGCTGGCGATAACCATTCCGTCCACGCTGCTGGGTATTTT
AGCCATCGGTATCTTCAGCTGGTTCCGCGGCAAAGACCTGGATCAGGACGAAGCCTTCCAGGCGTTTATTGCGCTCCCGG
AGAACCGCCATTACGTCTATGGCGATACGGCGACGCTGCTGGACAAAAAGCTGCCGACCAGCAACTGGATAGCAATGTGG
ATTTTTCTGGCCTCTATCGCCGTGGTGGCGCTGCTTGGCGCTTTTTCTGAACTGCGCCCGGCCTTTGACGGTAAACCACT
GTCGATGGTGCTGGTTATCCAGATGTTTATGCTGCTCTCCGGAGCGCTGATCATCATCATTACAAAAACTAACCCGGCAT
CGATTTCCAAAAATGAGGTCTTTCGTTCGGGAATGATCGCTATCGTCGCGGTGTACGGCATCGCGTGGATGGCGGAGACC
ATGTTTGGCGCGCACATGACTGAGATTAAAGGCGTGCTTGGTGAGATGGTAAAAGAGTATCCGTGGGCTTATGCCATCGT
GCTGCTACTGGTTTCGAAATTTGTGAACTCCCAGGCGGCAGCGCTGGCGGCGATTGTGCCAGTAGCCCTGGCCATCGGCG
TCGACCCGGCCTATATCGTGGCTTCTGCGCCGGCCTGCTATGGCTACTATATTCTGCCGACCTACCCAAGCGATCTGGCG
GCGATCCAGTTTGATCGCTCAGGGACCACCCATATCGGCCGCTTTGTGATTAACCACAGCTTTATCCTGCCCGGGCTCAT
CGGCGTCGGCGTCTCCTGCGTGTTTGGCTGGGTATTCGCGGCGATGTACGGCTTTCTGTAA

Upstream 100 bases:

>100_bases
ATAGCCGCATCTCATGCTTCTCCCGGTCTGGCGCAGAAATAATCCCGAGAGGCAGGTAGGGATCATTTTTAGATCTGAGT
GGACAGGCTCTGGGGGCTGC

Downstream 100 bases:

>100_bases
GAAGGACGGGCGCCGGCAGCGACGCCCGTTAAATCCGTGAGATTATTGCGCGAGGGGCAGACGACGATAGAGTTCAATCA
TGTCGGTCGCCAGATCCTGA

Product: anaerobic C4-dicarboxylate transporter

Products: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MEFAIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQPGKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEK
LLRRNPKYVSIVAPFVTCTLTILCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
NFTFNGKHLEFLDLLAITIPSTLLGILAIGIFSWFRGKDLDQDEAFQAFIALPENRHYVYGDTATLLDKKLPTSNWIAMW
IFLASIAVVALLGAFSELRPAFDGKPLSMVLVIQMFMLLSGALIIIITKTNPASISKNEVFRSGMIAIVAVYGIAWMAET
MFGAHMTEIKGVLGEMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYILPTYPSDLA
AIQFDRSGTTHIGRFVINHSFILPGLIGVGVSCVFGWVFAAMYGFL

Sequences:

>Translated_446_residues
MEFAIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQPGKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEK
LLRRNPKYVSIVAPFVTCTLTILCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
NFTFNGKHLEFLDLLAITIPSTLLGILAIGIFSWFRGKDLDQDEAFQAFIALPENRHYVYGDTATLLDKKLPTSNWIAMW
IFLASIAVVALLGAFSELRPAFDGKPLSMVLVIQMFMLLSGALIIIITKTNPASISKNEVFRSGMIAIVAVYGIAWMAET
MFGAHMTEIKGVLGEMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYILPTYPSDLA
AIQFDRSGTTHIGRFVINHSFILPGLIGVGVSCVFGWVFAAMYGFL
>Mature_446_residues
MEFAIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQPGKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEK
LLRRNPKYVSIVAPFVTCTLTILCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
NFTFNGKHLEFLDLLAITIPSTLLGILAIGIFSWFRGKDLDQDEAFQAFIALPENRHYVYGDTATLLDKKLPTSNWIAMW
IFLASIAVVALLGAFSELRPAFDGKPLSMVLVIQMFMLLSGALIIIITKTNPASISKNEVFRSGMIAIVAVYGIAWMAET
MFGAHMTEIKGVLGEMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYILPTYPSDLA
AIQFDRSGTTHIGRFVINHSFILPGLIGVGVSCVFGWVFAAMYGFL

Specific function: Responsible for the transport of C4-dicarboxylates from the periplasm across the inner membrane [H]

COG id: COG2704

COG function: function code R; Anaerobic C4-dicarboxylate transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dcuA/dcuB transporter (TC 2.A.13.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790565, Length=432, Percent_Identity=94.4444444444444, Blast_Score=763, Evalue=0.0,
Organism=Escherichia coli, GI1790580, Length=439, Percent_Identity=36.6742596810934, Blast_Score=265, Evalue=3e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004668 [H]

Pfam domain/function: PF03605 DcuA_DcuB [H]

EC number: NA

Molecular weight: Translated: 47879; Mature: 47879

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFAIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQPGKPPVDVMLVIIAVVA
CHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
ASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTILCGTGHVVYTILPIIYDV
HHHHEECCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
AIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLGNFTFNGKHLEFLDLLAITIP
HHHCCCCCCCCCHHHHHCCHHCHHHCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
STLLGILAIGIFSWFRGKDLDQDEAFQAFIALPENRHYVYGDTATLLDKKLPTSNWIAMW
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCEEECCHHHHHHCCCCCCHHHHHH
IFLASIAVVALLGAFSELRPAFDGKPLSMVLVIQMFMLLSGALIIIITKTNPASISKNEV
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHH
FRSGMIAIVAVYGIAWMAETMFGAHMTEIKGVLGEMVKEYPWAYAIVLLLVSKFVNSQAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHH
ALAAIVPVALAIGVDPAYIVASAPACYGYYILPTYPSDLAAIQFDRSGTTHIGRFVINHS
HHHHHHHHHHHHCCCCHHHEECCCCCCCEEEECCCCCCCEEEEECCCCCHHHHHHHHHCC
FILPGLIGVGVSCVFGWVFAAMYGFL
HHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEFAIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQPGKPPVDVMLVIIAVVA
CHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
ASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTILCGTGHVVYTILPIIYDV
HHHHEECCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
AIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLGNFTFNGKHLEFLDLLAITIP
HHHCCCCCCCCCHHHHHCCHHCHHHCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
STLLGILAIGIFSWFRGKDLDQDEAFQAFIALPENRHYVYGDTATLLDKKLPTSNWIAMW
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCEEECCHHHHHHCCCCCCHHHHHH
IFLASIAVVALLGAFSELRPAFDGKPLSMVLVIQMFMLLSGALIIIITKTNPASISKNEV
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHH
FRSGMIAIVAVYGIAWMAETMFGAHMTEIKGVLGEMVKEYPWAYAIVLLLVSKFVNSQAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHH
ALAAIVPVALAIGVDPAYIVASAPACYGYYILPTYPSDLAAIQFDRSGTTHIGRFVINHS
HHHHHHHHHHHHCCCCHHHEECCCCCCCEEEECCCCCCCEEEEECCCCCHHHHHHHHHCC
FILPGLIGVGVSCVFGWVFAAMYGFL
HHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Specific reaction: C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] = C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]