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The map label for this gene is metR [H]

Identifier: 238892425

GI number: 238892425

Start: 188724

End: 189677

Strand: Reverse

Name: metR [H]

Synonym: KP1_0185

Alternate gene names: 238892425

Gene position: 189677-188724 (Counterclockwise)

Preceding gene: 238892426

Following gene: 238892421

Centisome position: 3.61

GC content: 61.22

Gene sequence:

>954_bases
ATGATCGAAATTAAACATCTGAAAACGCTGCAAGCGTTGCGGAACAGCGGTTCACTGGCGGGTGCGGCAGCGGCCCTGCA
CCAGACCCAGTCCGCCTTGTCTCACCAGTTCAGCGATCTGGAACAGCGCCTTGGCTTTCGTCTCTTTGTGCGTAAGAGCC
AGCCGCTGCGTTTTACTCCTCAGGGGGAAATCCTCTTACAGCTGGCCAACCAGGTGCTGCCGCAGATCGCTCGCGCCCTG
CAGGACTGTAACGAACCACAGCAGACCCGGCTGAGGCTGGCTATCGAATGTCACAGCTGTATTCAGTGGCTGACGCCGGC
GCTGGAGAATTTCCGCGCCCGCTGGCCACATGTGGAGGTCGACTTCCATTCCGGCGTCACCTTCGACCCACAGCCTGCGC
TGCAGCAGGGTGAACTGGATCTGGTGATGACCTCCGATATTCTGCCGCGCAGCGGCCTGCACTACTCGCCGATGTTTGAT
TTTGAGGTGCGTCTGGTGCTGGCGCCCGAGCATCCGTTAGCTATGAAGACGCTGGTTACCCCGGAGGATCTGGCCGCGGA
AACGCTGTTGATTTATCCCGTGCAGCGCGGGCGCCTCGATATCTGGCGCCACTTCCTGCAGCCGGCCGGCATCAGCCCGC
ACCTGAAAAGCGTCGATAACACGCTGTTGTTGATCCAGATGGTGGCTGCCCGGATGGGCATCGCCGCGCTGCCGCACTGG
GTGGTGGAAAGCTTTGAACGCCAGGGCCTGGTGGTCACCAAAACCCTGGGTGAGGGATTATGGAGCCGGTTGTACGCGGC
CGTTCGCGATGGCGAACAGCGTCAACCGGTCACCGAAGCGTTTATTCGCTCTGCGCGGAACCACGCCTGCGATCATCTGC
CGTTTGTCCGGAGTGCGGAGCGACCCAGCGGCGATGGACCCACAGCGAGGCCAGGATCACCAACGCTCCGCTAA

Upstream 100 bases:

>100_bases
TATGACGAGTTTTAGACGTCCAGATGTTTACACATCCATAATTGACAGTTACTGTATATTCCTCAAGCGCAAATTGCTCA
TGGCGAAGTGAAGGACTTTC

Downstream 100 bases:

>100_bases
TAAAGCTCGGCCAGTGCGGCTGCTCCTGCCAGATGGCAAGGTTAACCAGCAGCCCGGCGGGGACATGGACGTTATTCATG
ATCCCCAGCGTGCCGGCATC

Product: regulator for metE and metH

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MIEIKHLKTLQALRNSGSLAGAAAALHQTQSALSHQFSDLEQRLGFRLFVRKSQPLRFTPQGEILLQLANQVLPQIARAL
QDCNEPQQTRLRLAIECHSCIQWLTPALENFRARWPHVEVDFHSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
FEVRLVLAPEHPLAMKTLVTPEDLAAETLLIYPVQRGRLDIWRHFLQPAGISPHLKSVDNTLLLIQMVAARMGIAALPHW
VVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQPVTEAFIRSARNHACDHLPFVRSAERPSGDGPTARPGSPTLR

Sequences:

>Translated_317_residues
MIEIKHLKTLQALRNSGSLAGAAAALHQTQSALSHQFSDLEQRLGFRLFVRKSQPLRFTPQGEILLQLANQVLPQIARAL
QDCNEPQQTRLRLAIECHSCIQWLTPALENFRARWPHVEVDFHSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
FEVRLVLAPEHPLAMKTLVTPEDLAAETLLIYPVQRGRLDIWRHFLQPAGISPHLKSVDNTLLLIQMVAARMGIAALPHW
VVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQPVTEAFIRSARNHACDHLPFVRSAERPSGDGPTARPGSPTLR
>Mature_317_residues
MIEIKHLKTLQALRNSGSLAGAAAALHQTQSALSHQFSDLEQRLGFRLFVRKSQPLRFTPQGEILLQLANQVLPQIARAL
QDCNEPQQTRLRLAIECHSCIQWLTPALENFRARWPHVEVDFHSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
FEVRLVLAPEHPLAMKTLVTPEDLAAETLLIYPVQRGRLDIWRHFLQPAGISPHLKSVDNTLLLIQMVAARMGIAALPHW
VVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQPVTEAFIRSARNHACDHLPFVRSAERPSGDGPTARPGSPTLR

Specific function: Control of the last step in methionine biosynthesis; metR is a positive activator of the metA, metE and metH genes. It is also a negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790262, Length=314, Percent_Identity=90.7643312101911, Blast_Score=593, Evalue=1e-171,
Organism=Escherichia coli, GI157672245, Length=236, Percent_Identity=26.6949152542373, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788887, Length=288, Percent_Identity=26.7361111111111, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787806, Length=290, Percent_Identity=23.7931034482759, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789204, Length=264, Percent_Identity=25.7575757575758, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI87082024, Length=176, Percent_Identity=30.6818181818182, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1787530, Length=213, Percent_Identity=25.8215962441315, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1790208, Length=301, Percent_Identity=27.2425249169435, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787879, Length=255, Percent_Identity=26.6666666666667, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1786401, Length=245, Percent_Identity=24.8979591836735, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1789506, Length=230, Percent_Identity=24.3478260869565, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI145693105, Length=240, Percent_Identity=24.1666666666667, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790399, Length=256, Percent_Identity=27.734375, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1789173, Length=188, Percent_Identity=27.1276595744681, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788706, Length=189, Percent_Identity=26.984126984127, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35558; Mature: 35558

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEIKHLKTLQALRNSGSLAGAAAALHQTQSALSHQFSDLEQRLGFRLFVRKSQPLRFTP
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEECC
QGEILLQLANQVLPQIARALQDCNEPQQTRLRLAIECHSCIQWLTPALENFRARWPHVEV
CHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
DFHSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFDFEVRLVLAPEHPLAMKTLVT
EECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHEECC
PEDLAAETLLIYPVQRGRLDIWRHFLQPAGISPHLKSVDNTLLLIQMVAARMGIAALPHW
CHHHHHCEEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQPVTEAFIRSARNHACDHLPFVRSAE
HHHHHHHCCEEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHCCC
RPSGDGPTARPGSPTLR
CCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIEIKHLKTLQALRNSGSLAGAAAALHQTQSALSHQFSDLEQRLGFRLFVRKSQPLRFTP
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEECC
QGEILLQLANQVLPQIARALQDCNEPQQTRLRLAIECHSCIQWLTPALENFRARWPHVEV
CHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
DFHSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFDFEVRLVLAPEHPLAMKTLVT
EECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHEECC
PEDLAAETLLIYPVQRGRLDIWRHFLQPAGISPHLKSVDNTLLLIQMVAARMGIAALPHW
CHHHHHCEEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQPVTEAFIRSARNHACDHLPFVRSAE
HHHHHHHCCEEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHCCC
RPSGDGPTARPGSPTLR
CCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]