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The map label for this gene is dapF [H]

Identifier: 238892409

GI number: 238892409

Start: 172448

End: 173275

Strand: Direct

Name: dapF [H]

Synonym: KP1_0168

Alternate gene names: 238892409

Gene position: 172448-173275 (Clockwise)

Preceding gene: 238892408

Following gene: 238892410

Centisome position: 3.29

GC content: 59.42

Gene sequence:

>828_bases
ATGATGCAGTTCTCTAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTTGACGCGGTAACGCAGAATGTTTTTTTCTC
GCCGGAGCTGATTCGCCGCCTGGCGGATCGCCACCTTGGCGTCGGGTTCGATCAGCTATTGGTCGTCGAACCGCCCTACG
ATCCGGATCTGGATTTCCACTACCGGATCTTTAACGCCGACGGCAGCGAAGTGTCGCAGTGCGGCAACGGCGCGCGCTGT
TTTGCCCGCTTCGTCCGGCTGAAAGGCCTGACCAATAAACGTGATATCCGCGTGAGTACGGCCAACGGGCGTATGGTATT
AAGCGTAACGGAAGACGATCTGGTGCGGGTGAACATGGGCGAGCCCAACTTCGAGCCGTCCCAGGTGCCGTTCCGCGCCA
ACAAAGCGGAAAAGACCTATATCATGCGTGCCGCCGAGCAGACAGTATTGTGCGGCGTGGTGTCCATGGGAAATCCGCAC
TGCGTCATCCAGGTTGATGATGTGGATACGGCAGCGGTGGAAACTCTCGGCCCGGTGATGGAAAGCCATGAGCGTTTTCC
GGAGCGCGCCAATATCGGCTTTATGCAGGTGGTCGGCCGGTCTCATATCCGTCTGCGCGTCTACGAACGCGGCGCGGGGG
AAACCCAGGCCTGCGGCAGCGGCGCTTGCGCCGCGGTGGCGGTGGGGATCCAGCAGGGGCTGCTGGCGGAAACGGTACGC
GTGGAATTACCCGGCGGTCGTCTTGATATCGCCTGGAAAGGCCCGGGCCAGCCGCTGTTTATGACCGGCCCGGCGGCCCA
TGTCTATGATGGATTTATTCATTTATGA

Upstream 100 bases:

>100_bases
TTCAGAGCGCAACGCCGGATCGTAACGATCGTGGCGATACCGGTGGTCCGAGCCAGGTCAATTACTAACGATAACGTTCT
GTTCCGCGCAACTGGAGTAG

Downstream 100 bases:

>100_bases
AACCAATCGGGGAAGAACAGCAGGAGGTTGCCAGCGCGTTGAACGATCGCGCAGTGGTGGACTATCTGTTGCAACATCCT
GAATTTTTTATTCGTAATGC

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MMQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQLLVVEPPYDPDLDFHYRIFNADGSEVSQCGNGARC
FARFVRLKGLTNKRDIRVSTANGRMVLSVTEDDLVRVNMGEPNFEPSQVPFRANKAEKTYIMRAAEQTVLCGVVSMGNPH
CVIQVDDVDTAAVETLGPVMESHERFPERANIGFMQVVGRSHIRLRVYERGAGETQACGSGACAAVAVGIQQGLLAETVR
VELPGGRLDIAWKGPGQPLFMTGPAAHVYDGFIHL

Sequences:

>Translated_275_residues
MMQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQLLVVEPPYDPDLDFHYRIFNADGSEVSQCGNGARC
FARFVRLKGLTNKRDIRVSTANGRMVLSVTEDDLVRVNMGEPNFEPSQVPFRANKAEKTYIMRAAEQTVLCGVVSMGNPH
CVIQVDDVDTAAVETLGPVMESHERFPERANIGFMQVVGRSHIRLRVYERGAGETQACGSGACAAVAVGIQQGLLAETVR
VELPGGRLDIAWKGPGQPLFMTGPAAHVYDGFIHL
>Mature_275_residues
MMQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQLLVVEPPYDPDLDFHYRIFNADGSEVSQCGNGARC
FARFVRLKGLTNKRDIRVSTANGRMVLSVTEDDLVRVNMGEPNFEPSQVPFRANKAEKTYIMRAAEQTVLCGVVSMGNPH
CVIQVDDVDTAAVETLGPVMESHERFPERANIGFMQVVGRSHIRLRVYERGAGETQACGSGACAAVAVGIQQGLLAETVR
VELPGGRLDIAWKGPGQPLFMTGPAAHVYDGFIHL

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=274, Percent_Identity=95.2554744525548, Blast_Score=545, Evalue=1e-156,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 30209; Mature: 30209

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQLLVVEPPYDPDLDFH
CCCCHHHCCCCCCEEEEEEECCCEEECHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEE
YRIFNADGSEVSQCGNGARCFARFVRLKGLTNKRDIRVSTANGRMVLSVTEDDLVRVNMG
EEEEECCCHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEEECCCCEEEEECC
EPNFEPSQVPFRANKAEKTYIMRAAEQTVLCGVVSMGNPHCVIQVDDVDTAAVETLGPVM
CCCCCCCCCCEECCCCCHHHEEHHHCCEEEEEEEECCCCEEEEEECCCCHHHHHHHHHHH
ESHERFPERANIGFMQVVGRSHIRLRVYERGAGETQACGSGACAAVAVGIQQGLLAETVR
HHHHHCCCCCCCCCEEEECCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCEEEEEE
VELPGGRLDIAWKGPGQPLFMTGPAAHVYDGFIHL
EECCCCEEEEEECCCCCEEEEECCHHHHHCCEEEC
>Mature Secondary Structure
MMQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQLLVVEPPYDPDLDFH
CCCCHHHCCCCCCEEEEEEECCCEEECHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEE
YRIFNADGSEVSQCGNGARCFARFVRLKGLTNKRDIRVSTANGRMVLSVTEDDLVRVNMG
EEEEECCCHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEEECCCCEEEEECC
EPNFEPSQVPFRANKAEKTYIMRAAEQTVLCGVVSMGNPHCVIQVDDVDTAAVETLGPVM
CCCCCCCCCCEECCCCCHHHEEHHHCCEEEEEEEECCCCEEEEEECCCCHHHHHHHHHHH
ESHERFPERANIGFMQVVGRSHIRLRVYERGAGETQACGSGACAAVAVGIQQGLLAETVR
HHHHHCCCCCCCCCEEEECCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCEEEEEE
VELPGGRLDIAWKGPGQPLFMTGPAAHVYDGFIHL
EECCCCEEEEEECCCCCEEEEECCHHHHHCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA