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The map label for this gene is btuB [H]

Identifier: 238892364

GI number: 238892364

Start: 117391

End: 119229

Strand: Direct

Name: btuB [H]

Synonym: KP1_0121

Alternate gene names: 238892364

Gene position: 117391-119229 (Clockwise)

Preceding gene: 238892363

Following gene: 238892365

Centisome position: 2.24

GC content: 55.95

Gene sequence:

>1839_bases
ATGATTAAAAAAGCTTCGCTGATGACGGCCTTATCCGTCACGGCATTTTCCGGCTGGGCGCAGGATAGCAATTCAGATAC
GTTGGTGGTGACAGCAAACCGTTTTCAACAGCCGGTCAATACCGTGCTGGCGCCGACCGACATTGTGACGCGCGATGACA
TCGACCGCTGGCAGTCCAAAGATTTAAACGATGTCATGCGTCGTCTTCCCGGGGTCGATATTGCCCGCAACGGCGGCATG
GGGCAGAGCGCTTCGCTGTATGTTCGGGGGACGGAGGCTCGTCACGTGCTGGTGCTGATCGACGGTGTGCCGATGGCGCG
TCCGGGGATCTCCAACGGCGTAGATATCAGTCAGATCCCTATCTCACTGGTCCAGCGGGTGGAATACATCCGCGGCCCGC
GCTCCGCGGTGTACGGCTCCGGAGCGATCGGCGGGGTGGTGAACATCATCACCATGACCGACGCTGAGCGTTCGCAGATC
AACGCCGGCGCGGGTACGAACGGCTATCAGTCCTATGACGGCGCCTTTAACAAGCGGTTTGGCGATACGTTGGTTACCGC
TGCTGGCGCCTATCAGACCACCAAAGGGTTTAACGTCCAGCCGAATTCCTCTTACAGCGGCGACAGCGATCGCGACGGCT
ACCGCAATAAAATGCTGTGGGGCGGGGTACAGCATCAGTTCGATGACAACTTCTCGGGGTTCTTCCGCGGCTATGGTTAT
TCTGCTAACGCTGACTATGACCAGGGTAACTGGGGCTACGCAGGTGGAAACGATGAAGATCAATCCTATACCCAATCCTG
GGATACCGGTCTGCACTACCACTCCGGAATTTACTCCTCCCAGCTGATTGCTAACTATCAGCGCATCAAAGATTACAACT
ACAGCAGCGACGCTGGCCGCTATGCCGCGGGCACCACCCTGGATGATATGGAACAGCGCTATATCCAGTGGGGAAATAAT
GTTGTGGTAGGCCATGGGGCAGTGAGCGGCGGCGTTGACTGGAAACAAGAGAAGCTGAAATCCAGCGGAACGACCAGTAC
CGACGTGTATAAGCGTGACACCACCGGTCTTTATCTGACGGGACAGCAGCAGATTGACAGCGTGACGCTGGAAGCTTCCG
GCCGTGAGGATCATGACGAGCAGTTTGGCTGGCACGGTACCTGGCAGACGGCCGCCGGCTGGGAATTTATCGACGGTTAT
CGGACAACGCTCTCGTACGGCACAGGATTCCTCGCCCCCTCCCTCGGGCAGCAGTACGGCGCAGAACGCTTTGGCATCGC
CTCTAACCCGAATCTGAAGCCAGAGGAGTCGAAGCAATGGGAAGCGGGCCTTGAAGGGTTAACGGGGCCGGTCGATTGGC
GACTCTCCGCGTATCGCTATGAGATTCAAAACCTCATCGATTACGACAACAACGCCTATTACAACGTCAAGTCGGCGACG
ATTAAGGGGCTGGAGTGGACGGGGAATATAACCACCGGGCCGGTGGAGCACCATTTGACGCTGCAGTATGTTGACCCTCG
CGATGATGAAACCAATAAGATCCTCTATCGCCGGGCGAAGCAGCAGGTGAAATACGAGCTGAACGGCCAGGTCTACGATC
TGGGGTGGGATGTGACGTATCACTACATCGGCAAGCGTTACGATTATGACTACGACAACTCGCGTACCGTCAATATGGGT
GGGTTGAGCCTCTGGGATGTCGGTTTATCGTATCCCGTCACCTCACACCTGACAGTTCGTGGTAAAATAGCCAACCTGTT
CGATAAAGATTACGAGACAGTTTATGGCTACCAATCTGCAGGACGGGAATACACCTTGTCTGGCAGCTACACCTTCTGA

Upstream 100 bases:

>100_bases
CAAGCCCGAAGACCTGCCGGAATACGTCGCACTGGGTTTTATCGTCGCGAGCAACTGATAAAACCTGCGGCATCCTTTTT
CTGTTTCGGATGCTTTTACA

Downstream 100 bases:

>100_bases
TCCACGTCCCACCGTGCTGGTGTTTGATTCCGGCGTCGGTGGGTTGTCGGTTTATAATGAGATTCGGCAGCTGCTGCCGA
ATCTGCATTACATCTATGCT

Product: vitamin B12/cobalamin outer membrane transporter

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 612; Mature: 612

Protein sequence:

>612_residues
MIKKASLMTALSVTAFSGWAQDSNSDTLVVTANRFQQPVNTVLAPTDIVTRDDIDRWQSKDLNDVMRRLPGVDIARNGGM
GQSASLYVRGTEARHVLVLIDGVPMARPGISNGVDISQIPISLVQRVEYIRGPRSAVYGSGAIGGVVNIITMTDAERSQI
NAGAGTNGYQSYDGAFNKRFGDTLVTAAGAYQTTKGFNVQPNSSYSGDSDRDGYRNKMLWGGVQHQFDDNFSGFFRGYGY
SANADYDQGNWGYAGGNDEDQSYTQSWDTGLHYHSGIYSSQLIANYQRIKDYNYSSDAGRYAAGTTLDDMEQRYIQWGNN
VVVGHGAVSGGVDWKQEKLKSSGTTSTDVYKRDTTGLYLTGQQQIDSVTLEASGREDHDEQFGWHGTWQTAAGWEFIDGY
RTTLSYGTGFLAPSLGQQYGAERFGIASNPNLKPEESKQWEAGLEGLTGPVDWRLSAYRYEIQNLIDYDNNAYYNVKSAT
IKGLEWTGNITTGPVEHHLTLQYVDPRDDETNKILYRRAKQQVKYELNGQVYDLGWDVTYHYIGKRYDYDYDNSRTVNMG
GLSLWDVGLSYPVTSHLTVRGKIANLFDKDYETVYGYQSAGREYTLSGSYTF

Sequences:

>Translated_612_residues
MIKKASLMTALSVTAFSGWAQDSNSDTLVVTANRFQQPVNTVLAPTDIVTRDDIDRWQSKDLNDVMRRLPGVDIARNGGM
GQSASLYVRGTEARHVLVLIDGVPMARPGISNGVDISQIPISLVQRVEYIRGPRSAVYGSGAIGGVVNIITMTDAERSQI
NAGAGTNGYQSYDGAFNKRFGDTLVTAAGAYQTTKGFNVQPNSSYSGDSDRDGYRNKMLWGGVQHQFDDNFSGFFRGYGY
SANADYDQGNWGYAGGNDEDQSYTQSWDTGLHYHSGIYSSQLIANYQRIKDYNYSSDAGRYAAGTTLDDMEQRYIQWGNN
VVVGHGAVSGGVDWKQEKLKSSGTTSTDVYKRDTTGLYLTGQQQIDSVTLEASGREDHDEQFGWHGTWQTAAGWEFIDGY
RTTLSYGTGFLAPSLGQQYGAERFGIASNPNLKPEESKQWEAGLEGLTGPVDWRLSAYRYEIQNLIDYDNNAYYNVKSAT
IKGLEWTGNITTGPVEHHLTLQYVDPRDDETNKILYRRAKQQVKYELNGQVYDLGWDVTYHYIGKRYDYDYDNSRTVNMG
GLSLWDVGLSYPVTSHLTVRGKIANLFDKDYETVYGYQSAGREYTLSGSYTF
>Mature_612_residues
MIKKASLMTALSVTAFSGWAQDSNSDTLVVTANRFQQPVNTVLAPTDIVTRDDIDRWQSKDLNDVMRRLPGVDIARNGGM
GQSASLYVRGTEARHVLVLIDGVPMARPGISNGVDISQIPISLVQRVEYIRGPRSAVYGSGAIGGVVNIITMTDAERSQI
NAGAGTNGYQSYDGAFNKRFGDTLVTAAGAYQTTKGFNVQPNSSYSGDSDRDGYRNKMLWGGVQHQFDDNFSGFFRGYGY
SANADYDQGNWGYAGGNDEDQSYTQSWDTGLHYHSGIYSSQLIANYQRIKDYNYSSDAGRYAAGTTLDDMEQRYIQWGNN
VVVGHGAVSGGVDWKQEKLKSSGTTSTDVYKRDTTGLYLTGQQQIDSVTLEASGREDHDEQFGWHGTWQTAAGWEFIDGY
RTTLSYGTGFLAPSLGQQYGAERFGIASNPNLKPEESKQWEAGLEGLTGPVDWRLSAYRYEIQNLIDYDNNAYYNVKSAT
IKGLEWTGNITTGPVEHHLTLQYVDPRDDETNKILYRRAKQQVKYELNGQVYDLGWDVTYHYIGKRYDYDYDNSRTVNMG
GLSLWDVGLSYPVTSHLTVRGKIANLFDKDYETVYGYQSAGREYTLSGSYTF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=622, Percent_Identity=57.2347266881029, Blast_Score=691, Evalue=0.0,
Organism=Escherichia coli, GI1788478, Length=692, Percent_Identity=24.5664739884393, Blast_Score=138, Evalue=1e-33,
Organism=Escherichia coli, GI1786798, Length=688, Percent_Identity=24.4186046511628, Blast_Score=98, Evalue=2e-21,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010101
- InterPro:   IPR010917 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 68110; Mature: 68110

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00430 TONB_DEPENDENT_REC_1 ; PS01156 TONB_DEPENDENT_REC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKASLMTALSVTAFSGWAQDSNSDTLVVTANRFQQPVNTVLAPTDIVTRDDIDRWQSK
CCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHCCHHHHCCCCCCCCCCCHHHHHCC
DLNDVMRRLPGVDIARNGGMGQSASLYVRGTEARHVLVLIDGVPMARPGISNGVDISQIP
CHHHHHHHCCCCEEECCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCCCHHHHH
ISLVQRVEYIRGPRSAVYGSGAIGGVVNIITMTDAERSQINAGAGTNGYQSYDGAFNKRF
HHHHHHHHHHCCCCHHEECCCCCCCEEEEEEECCCCHHHCCCCCCCCCCCCCCCHHHHHC
GDTLVTAAGAYQTTKGFNVQPNSSYSGDSDRDGYRNKMLWGGVQHQFDDNFSGFFRGYGY
CCHHEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHCCEEECCCCCCCCCCCCHHHHCCCC
SANADYDQGNWGYAGGNDEDQSYTQSWDTGLHYHSGIYSSQLIANYQRIKDYNYSSDAGR
CCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
YAAGTTLDDMEQRYIQWGNNVVVGHGAVSGGVDWKQEKLKSSGTTSTDVYKRDTTGLYLT
EECCCCHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHCCCCCCCEEEECCCCCEEEE
GQQQIDSVTLEASGREDHDEQFGWHGTWQTAAGWEFIDGYRTTLSYGTGFLAPSLGQQYG
CCCCCCEEEEEECCCCCCHHCCCCCCCCCCCCCCHHHCCHHHHHHCCCCCCCCHHHHHHC
AERFGIASNPNLKPEESKQWEAGLEGLTGPVDWRLSAYRYEIQNLIDYDNNAYYNVKSAT
CCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCE
IKGLEWTGNITTGPVEHHLTLQYVDPRDDETNKILYRRAKQQVKYELNGQVYDLGWDVTY
EECEEEECCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHEEEEECCEEEEECCCEEE
HYIGKRYDYDYDNSRTVNMGGLSLWDVGLSYPVTSHLTVRGKIANLFDKDYETVYGYQSA
EECCCEECCCCCCCEEEEECCEEEEECCCCCCCCCEEEEEEHHHHHHHCCHHHHHCHHHC
GREYTLSGSYTF
CCEEEECCCCCC
>Mature Secondary Structure
MIKKASLMTALSVTAFSGWAQDSNSDTLVVTANRFQQPVNTVLAPTDIVTRDDIDRWQSK
CCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHCCHHHHCCCCCCCCCCCHHHHHCC
DLNDVMRRLPGVDIARNGGMGQSASLYVRGTEARHVLVLIDGVPMARPGISNGVDISQIP
CHHHHHHHCCCCEEECCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCCCHHHHH
ISLVQRVEYIRGPRSAVYGSGAIGGVVNIITMTDAERSQINAGAGTNGYQSYDGAFNKRF
HHHHHHHHHHCCCCHHEECCCCCCCEEEEEEECCCCHHHCCCCCCCCCCCCCCCHHHHHC
GDTLVTAAGAYQTTKGFNVQPNSSYSGDSDRDGYRNKMLWGGVQHQFDDNFSGFFRGYGY
CCHHEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHCCEEECCCCCCCCCCCCHHHHCCCC
SANADYDQGNWGYAGGNDEDQSYTQSWDTGLHYHSGIYSSQLIANYQRIKDYNYSSDAGR
CCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
YAAGTTLDDMEQRYIQWGNNVVVGHGAVSGGVDWKQEKLKSSGTTSTDVYKRDTTGLYLT
EECCCCHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHCCCCCCCEEEECCCCCEEEE
GQQQIDSVTLEASGREDHDEQFGWHGTWQTAAGWEFIDGYRTTLSYGTGFLAPSLGQQYG
CCCCCCEEEEEECCCCCCHHCCCCCCCCCCCCCCHHHCCHHHHHHCCCCCCCCHHHHHHC
AERFGIASNPNLKPEESKQWEAGLEGLTGPVDWRLSAYRYEIQNLIDYDNNAYYNVKSAT
CCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCE
IKGLEWTGNITTGPVEHHLTLQYVDPRDDETNKILYRRAKQQVKYELNGQVYDLGWDVTY
EECEEEECCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHEEEEECCEEEEECCCEEE
HYIGKRYDYDYDNSRTVNMGGLSLWDVGLSYPVTSHLTVRGKIANLFDKDYETVYGYQSA
EECCCEECCCCCCCEEEEECCEEEEECCCCCCCCCEEEEEEHHHHHHHCCHHHHHCHHHC
GREYTLSGSYTF
CCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA