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The map label for this gene is aes [H]

Identifier: 238892289

GI number: 238892289

Start: 41625

End: 42542

Strand: Reverse

Name: aes [H]

Synonym: KP1_0036

Alternate gene names: 238892289

Gene position: 42542-41625 (Counterclockwise)

Preceding gene: 238892292

Following gene: 238892283

Centisome position: 0.81

GC content: 60.46

Gene sequence:

>918_bases
ATGGCGCTGGAAAAAGGGATTGCTTCGCTGGTTGAGGCATTTATTGCGGCAGGTCGTCCGTCATCACGCGATCAGCATAT
TGATGATCGGAGGGCCGGCTATATCGCCAGTGCGGTACTCGCCGGGGAGACGGAAACGCGAGTGCGGGTGGAGGATATCA
CCCTGGAGGGTATGCACTTCCGCGTCGTTTCACCGCCCACTGCCGACGGCCTGCTACCCACGCTTATCTACTACCACGGC
GGCTGTTTTGTCAGCGGCGGCTTTACCACCCACGATAACCAACTCCGTCAGTTAGCCATGTTCAGCGGTTGCCGGGTGAT
CGCTGTACAGTACCGCCTGGCGCCGGAGCATACCTTTCCGGCGGCGCACGATGACGCAGAACAAGGTGTCATGATTATCC
ACCGGCACGCGGAGCAGCTTGGCGTTGACGCATCACGTATCACCCTGGCTGGCGACAGTGCCGGCGGGCACCTCGCTCTG
GTCACGGCCCTGCGCCTGAAAGCGAAAGCGGCGTGGCAACCCGCGCAACTGATCCTGATTTATCCGATGCTTGACCCCAC
CGCCAGCATGGCGAGCTACCTCAGCAATGGCGATGACTATGTGATTACCCGGGACACGCTACTGAGCGGCTACGAAATGT
ACCTCGCCTCGACGCCGGCAAACCATCCGGATGCCTGTCCGCTGTGGCGCGAAGATTTTAACGGGCTGCCACCGGTACAC
ATTCTGACGGCGGAGTTCGATCCTTTACGTGATGAGGGCGAAGTGCTGTACCGAAGGCTGACGGAGCAAGGGGTGGAGAG
TTCCTGTCAGCGCTATCTGGGGGTGATCCACGGCTTCTTTCAGCTGGGAGGCGTCAGCAACGCCGCGCGTGATGCCATGC
GGGATATCGCCTGGCGTGTGGCATCGCCAGGACGATAA

Upstream 100 bases:

>100_bases
GAGCCGCTTTCCATAATGCACAGGCGACCTCAATAGGCCTGTCTTTTCGCCATAATCCAGGCTATAACACTGGATATTAA
AACAGTATAAGGAACTTCAT

Downstream 100 bases:

>100_bases
CTGCCGCAGGCGCTTACTCTTCCTCGTTGCCGCCCTCTTCGTACGAGCCAAAGGGCTTCGCCGGCAGCACGATGTAGATC
CCCTCGAAAACGGCGCCCTG

Product: acetyl esterase

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MALEKGIASLVEAFIAAGRPSSRDQHIDDRRAGYIASAVLAGETETRVRVEDITLEGMHFRVVSPPTADGLLPTLIYYHG
GCFVSGGFTTHDNQLRQLAMFSGCRVIAVQYRLAPEHTFPAAHDDAEQGVMIIHRHAEQLGVDASRITLAGDSAGGHLAL
VTALRLKAKAAWQPAQLILIYPMLDPTASMASYLSNGDDYVITRDTLLSGYEMYLASTPANHPDACPLWREDFNGLPPVH
ILTAEFDPLRDEGEVLYRRLTEQGVESSCQRYLGVIHGFFQLGGVSNAARDAMRDIAWRVASPGR

Sequences:

>Translated_305_residues
MALEKGIASLVEAFIAAGRPSSRDQHIDDRRAGYIASAVLAGETETRVRVEDITLEGMHFRVVSPPTADGLLPTLIYYHG
GCFVSGGFTTHDNQLRQLAMFSGCRVIAVQYRLAPEHTFPAAHDDAEQGVMIIHRHAEQLGVDASRITLAGDSAGGHLAL
VTALRLKAKAAWQPAQLILIYPMLDPTASMASYLSNGDDYVITRDTLLSGYEMYLASTPANHPDACPLWREDFNGLPPVH
ILTAEFDPLRDEGEVLYRRLTEQGVESSCQRYLGVIHGFFQLGGVSNAARDAMRDIAWRVASPGR
>Mature_304_residues
ALEKGIASLVEAFIAAGRPSSRDQHIDDRRAGYIASAVLAGETETRVRVEDITLEGMHFRVVSPPTADGLLPTLIYYHGG
CFVSGGFTTHDNQLRQLAMFSGCRVIAVQYRLAPEHTFPAAHDDAEQGVMIIHRHAEQLGVDASRITLAGDSAGGHLALV
TALRLKAKAAWQPAQLILIYPMLDPTASMASYLSNGDDYVITRDTLLSGYEMYLASTPANHPDACPLWREDFNGLPPVHI
LTAEFDPLRDEGEVLYRRLTEQGVESSCQRYLGVIHGFFQLGGVSNAARDAMRDIAWRVASPGR

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21328446, Length=123, Percent_Identity=37.3983739837398, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI206597554, Length=298, Percent_Identity=26.1744966442953, Blast_Score=76, Evalue=4e-14,
Organism=Escherichia coli, GI1786682, Length=225, Percent_Identity=34.6666666666667, Blast_Score=119, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI71996133, Length=293, Percent_Identity=27.9863481228669, Blast_Score=103, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI72001146, Length=326, Percent_Identity=26.9938650306748, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17567059, Length=139, Percent_Identity=35.2517985611511, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17540028, Length=112, Percent_Identity=35.7142857142857, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI115533412, Length=135, Percent_Identity=31.1111111111111, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI115533410, Length=135, Percent_Identity=31.1111111111111, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24656084, Length=110, Percent_Identity=34.5454545454545, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24656076, Length=110, Percent_Identity=34.5454545454545, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI20130169, Length=110, Percent_Identity=34.5454545454545, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 33330; Mature: 33199

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS01174 LIPASE_GDXG_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALEKGIASLVEAFIAAGRPSSRDQHIDDRRAGYIASAVLAGETETRVRVEDITLEGMHF
CCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEECCEEE
RVVSPPTADGLLPTLIYYHGGCFVSGGFTTHDNQLRQLAMFSGCRVIAVQYRLAPEHTFP
EEECCCCCCCHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCCEEEEEEEEECCCCCCC
AAHDDAEQGVMIIHRHAEQLGVDASRITLAGDSAGGHLALVTALRLKAKAAWQPAQLILI
CCCCCCCCCEEEEEECHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEE
YPMLDPTASMASYLSNGDDYVITRDTLLSGYEMYLASTPANHPDACPLWREDFNGLPPVH
EECCCCHHHHHHHHCCCCCEEEEHHHHHCCCEEEEEECCCCCCCCCCCHHHHCCCCCCEE
ILTAEFDPLRDEGEVLYRRLTEQGVESSCQRYLGVIHGFFQLGGVSNAARDAMRDIAWRV
EEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
ASPGR
CCCCC
>Mature Secondary Structure 
ALEKGIASLVEAFIAAGRPSSRDQHIDDRRAGYIASAVLAGETETRVRVEDITLEGMHF
CHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEECCEEE
RVVSPPTADGLLPTLIYYHGGCFVSGGFTTHDNQLRQLAMFSGCRVIAVQYRLAPEHTFP
EEECCCCCCCHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCCEEEEEEEEECCCCCCC
AAHDDAEQGVMIIHRHAEQLGVDASRITLAGDSAGGHLALVTALRLKAKAAWQPAQLILI
CCCCCCCCCEEEEEECHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEE
YPMLDPTASMASYLSNGDDYVITRDTLLSGYEMYLASTPANHPDACPLWREDFNGLPPVH
EECCCCHHHHHHHHCCCCCEEEEHHHHHCCCEEEEEECCCCCCCCCCCHHHHCCCCCCEE
ILTAEFDPLRDEGEVLYRRLTEQGVESSCQRYLGVIHGFFQLGGVSNAARDAMRDIAWRV
EEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
ASPGR
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA