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The map label for this gene is metK [H]

Identifier: 238028903

GI number: 238028903

Start: 3829330

End: 3830520

Strand: Reverse

Name: metK [H]

Synonym: bglu_1g33680

Alternate gene names: 238028903

Gene position: 3830520-3829330 (Counterclockwise)

Preceding gene: 238028913

Following gene: 238028901

Centisome position: 98.05

GC content: 65.49

Gene sequence:

>1191_bases
GTGGCAAACGATTATCTCTTCACGTCCGAATCCGTTTCCGAAGGCCATCCGGACAAAGTTGCCGACCAGATCTCCGACGC
GATTCTCGATGCCATCCTCACGCAGGACAAATACTCGCGTGTTGCCGCCGAGACGCTGTGCAACACGGGGCTTGTCGTGC
TGGCGGGTGAGATCACCACGACCGCCAACATCGACTACATCCAGATCGCGCGCGACACGATCCGCCGCATCGGCTACGAC
AACACCGACTTCGGCATCGACTACAAGGGCTGCGCGGTGCTCGTCGCCTACGACAAGCAGTCGCCGGACATCGCGCAAGG
CGTTGATCGCGCGCATGACAACAACCTCGACCAGGGTGCCGGTGACCAGGGCCTGATGTTCGGTTACGCGTGCGATGAAA
CGCCCGAACTGATGCCGCTGCCGATTTACCTGGCGCACCGTCTCGTCGAGCGTCAGGCCAATCTGCGCCGCGACGGCCGC
CTGAGCTGGCTGCGTCCCGATGCGAAGTCGCAGGTCACGGTCCGCTACGTCGACGGCAAGCCGCACGCGATCGACACCGT
CGTGCTGTCGACGCAGCACGCCCCGGACATTGATCTGCCGCAATTGCGCGAAGCCGTGATCGAGGAGATCATCAAGCCGA
CGCTGCCGGCCGAATTGATCAAGGGCGACATCAAGTTCCTGGTGAACCCGACCGGCCGTTTCGTGATCGGTGGCCCGCAG
GGCGACTGCGGCCTGACGGGCCGCAAGATCATCGTCGATACCTACGGCGGTGCCGCGCCGCATGGCGGCGGCGCGTTCTC
GGGCAAGGACCCGTCGAAGGTCGACCGCTCGGCCGCCTACGCGGGCCGCTACGTCGCCAAGAACATCGTGGCCGCGGGCC
TGGCCTCGCGCGCGCTGATCCAGGTGTCGTATGCGATCGGCGTGGCCGAGCCGACCTCGGTGATGGTCAACACGTTCGGC
ACCGGCAAGGTCTCGGACGAGACGATCACGAAGCTCGTGCGCGAGCACTTCGACCTGCGTCCGAAGGGCATCGTGCAGAT
GCTCGATCTGCTGCGCCCGATCTACGAGAAGACGGCGGCCTACGGCCACTTCGGCCGTGCCGAGCCGGAGTTCTCTTGGG
AAGCGACCGACAAGGCGCTGGTCCTCGCCGAAGCAGCGGGCGTCGCCCCCGCCGTCGCGCAGCCGGCATGA

Upstream 100 bases:

>100_bases
TGCCTGTCCGGGTCAAGGAATCCGCTAAAGCGTCGCCACTTCATCCGGTTCCGAAGCTGGCTACGTGAATGCAACCGTAA
CCACCAAGGAGCTTAGCCAA

Downstream 100 bases:

>100_bases
AACGCGCATGATGCGCCGCGTTCCGATTTGGGCCTGAACGGCCTGAATGCAAAAAACCCCGGCTTGCCGGGGTTTTTTCT
TGGGCGCGCGCGGCGTGCAG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MANDYLFTSESVSEGHPDKVADQISDAILDAILTQDKYSRVAAETLCNTGLVVLAGEITTTANIDYIQIARDTIRRIGYD
NTDFGIDYKGCAVLVAYDKQSPDIAQGVDRAHDNNLDQGAGDQGLMFGYACDETPELMPLPIYLAHRLVERQANLRRDGR
LSWLRPDAKSQVTVRYVDGKPHAIDTVVLSTQHAPDIDLPQLREAVIEEIIKPTLPAELIKGDIKFLVNPTGRFVIGGPQ
GDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALIQVSYAIGVAEPTSVMVNTFG
TGKVSDETITKLVREHFDLRPKGIVQMLDLLRPIYEKTAAYGHFGRAEPEFSWEATDKALVLAEAAGVAPAVAQPA

Sequences:

>Translated_396_residues
MANDYLFTSESVSEGHPDKVADQISDAILDAILTQDKYSRVAAETLCNTGLVVLAGEITTTANIDYIQIARDTIRRIGYD
NTDFGIDYKGCAVLVAYDKQSPDIAQGVDRAHDNNLDQGAGDQGLMFGYACDETPELMPLPIYLAHRLVERQANLRRDGR
LSWLRPDAKSQVTVRYVDGKPHAIDTVVLSTQHAPDIDLPQLREAVIEEIIKPTLPAELIKGDIKFLVNPTGRFVIGGPQ
GDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALIQVSYAIGVAEPTSVMVNTFG
TGKVSDETITKLVREHFDLRPKGIVQMLDLLRPIYEKTAAYGHFGRAEPEFSWEATDKALVLAEAAGVAPAVAQPA
>Mature_395_residues
ANDYLFTSESVSEGHPDKVADQISDAILDAILTQDKYSRVAAETLCNTGLVVLAGEITTTANIDYIQIARDTIRRIGYDN
TDFGIDYKGCAVLVAYDKQSPDIAQGVDRAHDNNLDQGAGDQGLMFGYACDETPELMPLPIYLAHRLVERQANLRRDGRL
SWLRPDAKSQVTVRYVDGKPHAIDTVVLSTQHAPDIDLPQLREAVIEEIIKPTLPAELIKGDIKFLVNPTGRFVIGGPQG
DCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALIQVSYAIGVAEPTSVMVNTFGT
GKVSDETITKLVREHFDLRPKGIVQMLDLLRPIYEKTAAYGHFGRAEPEFSWEATDKALVLAEAAGVAPAVAQPA

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=380, Percent_Identity=58.9473684210526, Blast_Score=431, Evalue=1e-121,
Organism=Homo sapiens, GI5174529, Length=380, Percent_Identity=57.1052631578947, Blast_Score=419, Evalue=1e-117,
Organism=Escherichia coli, GI1789311, Length=384, Percent_Identity=70.5729166666667, Blast_Score=540, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI17538498, Length=397, Percent_Identity=55.919395465995, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17538494, Length=376, Percent_Identity=57.4468085106383, Blast_Score=422, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI25145633, Length=380, Percent_Identity=57.3684210526316, Blast_Score=421, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17551082, Length=389, Percent_Identity=55.2699228791774, Blast_Score=418, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI32565911, Length=349, Percent_Identity=54.4412607449857, Blast_Score=361, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI32565905, Length=376, Percent_Identity=52.3936170212766, Blast_Score=358, Evalue=4e-99,
Organism=Caenorhabditis elegans, GI25145635, Length=332, Percent_Identity=55.7228915662651, Blast_Score=352, Evalue=1e-97,
Organism=Caenorhabditis elegans, GI32565903, Length=301, Percent_Identity=56.8106312292359, Blast_Score=320, Evalue=7e-88,
Organism=Caenorhabditis elegans, GI32565909, Length=84, Percent_Identity=55.952380952381, Blast_Score=106, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6323209, Length=378, Percent_Identity=59.2592592592593, Blast_Score=429, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6320710, Length=379, Percent_Identity=58.5751978891821, Blast_Score=429, Evalue=1e-121,
Organism=Drosophila melanogaster, GI45552159, Length=376, Percent_Identity=55.5851063829787, Blast_Score=393, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580529, Length=376, Percent_Identity=55.5851063829787, Blast_Score=393, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580537, Length=376, Percent_Identity=55.5851063829787, Blast_Score=393, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580533, Length=376, Percent_Identity=55.5851063829787, Blast_Score=393, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580535, Length=376, Percent_Identity=55.5851063829787, Blast_Score=393, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580531, Length=376, Percent_Identity=55.5851063829787, Blast_Score=393, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580527, Length=374, Percent_Identity=55.0802139037433, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580525, Length=374, Percent_Identity=55.0802139037433, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580541, Length=375, Percent_Identity=52.8, Blast_Score=365, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580539, Length=119, Percent_Identity=54.6218487394958, Blast_Score=132, Evalue=4e-31,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 42680; Mature: 42549

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANDYLFTSESVSEGHPDKVADQISDAILDAILTQDKYSRVAAETLCNTGLVVLAGEITT
CCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECEEE
TANIDYIQIARDTIRRIGYDNTDFGIDYKGCAVLVAYDKQSPDIAQGVDRAHDNNLDQGA
ECCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCC
GDQGLMFGYACDETPELMPLPIYLAHRLVERQANLRRDGRLSWLRPDAKSQVTVRYVDGK
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEEEEECCC
PHAIDTVVLSTQHAPDIDLPQLREAVIEEIIKPTLPAELIKGDIKFLVNPTGRFVIGGPQ
CCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCEEEEECCCCCEEEECCC
GDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALI
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
QVSYAIGVAEPTSVMVNTFGTGKVSDETITKLVREHFDLRPKGIVQMLDLLRPIYEKTAA
EEEHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
YGHFGRAEPEFSWEATDKALVLAEAAGVAPAVAQPA
HCCCCCCCCCCCCCCCCCEEEEEHHCCCCCCCCCCC
>Mature Secondary Structure 
ANDYLFTSESVSEGHPDKVADQISDAILDAILTQDKYSRVAAETLCNTGLVVLAGEITT
CCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECEEE
TANIDYIQIARDTIRRIGYDNTDFGIDYKGCAVLVAYDKQSPDIAQGVDRAHDNNLDQGA
ECCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCC
GDQGLMFGYACDETPELMPLPIYLAHRLVERQANLRRDGRLSWLRPDAKSQVTVRYVDGK
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEEEEECCC
PHAIDTVVLSTQHAPDIDLPQLREAVIEEIIKPTLPAELIKGDIKFLVNPTGRFVIGGPQ
CCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCEEEEECCCCCEEEECCC
GDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALI
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
QVSYAIGVAEPTSVMVNTFGTGKVSDETITKLVREHFDLRPKGIVQMLDLLRPIYEKTAA
EEEHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
YGHFGRAEPEFSWEATDKALVLAEAAGVAPAVAQPA
HCCCCCCCCCCCCCCCCCEEEEEHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA