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The map label for this gene is cca [H]

Identifier: 238028856

GI number: 238028856

Start: 3778694

End: 3779941

Strand: Reverse

Name: cca [H]

Synonym: bglu_1g33210

Alternate gene names: 238028856

Gene position: 3779941-3778694 (Counterclockwise)

Preceding gene: 238028857

Following gene: 238028852

Centisome position: 96.76

GC content: 72.76

Gene sequence:

>1248_bases
ATGAACATCTATGTGGTAGGCGGTGCGATCCGCGATGCCCTGCTCGGCCTGCCGGTGCAGGACCGCGACTACGTGGTGGT
GGGCGCCACGCCGGAGCAGCTGGCCGCGCGCGGCTTCCGCCCGGTGGGCAAGGATTTCCCGGTGTTCCTGCACCCGCAGA
CGCACGAGGAGTACGCGCTCGCGCGCACCGAGCGCAAGACGGCGGCCGGCTATCACGGCTTCCAGTTCCATTACGCGCCG
GACGTGACGCTCGACGAGGATCTGGCGCGCCGCGACCTGACGATCAACGCGATGGCGCGCGAGATCGATCCGGACGGCCG
GATCACGGGCGAGCTGATCGACCCGTTCGGCGGGCGCGCCGATCTGGCCGCGAAGCGGTTCCGCCACGTCGGGCCGGCGT
TCGTCGAGGACCCGGTGCGGATTCTGCGCGTCGCGCGCTTCGCGGCCCGTTTCGCCGATTTCACGGTGGCGCCCGAGACG
CTCGCGCTGATGCGCGAGATGGTGGCGGCGGGCGAGGTGGACGCGCTGGTGCCGGAGCGGGTCTGGCAGGAGGTGTCGCG
CGGGCTGATGGAGGCGAAGCCGTCGCGCATGTTCGCGGTGCTGCGCGAGTGCGGTGCGCTGGCGCGCATCCTGCCCGAGG
TGGACGCGCTGTTCGGCGTGCCGCAGCGCGCCGACTACCACCCCGAGGTGGATACCGGGGTGCACGTGATGATGGTGATC
GATCACGCGGCCAAGCATGCCTATTCGCTGCCGGTGCGGTTCGCCGCGCTGACCCACGATCTCGGCAAGGCCACCACGCC
GGCCGAGGTGCTGCCGCGCCACATCGGCCACGAGGGCCGCAGCGTCGATCTGCTCAGGCCGCTCTGCGAGCGGCTGCGCG
TGCCGAACGAGTGTCGCGACCTGGCGCTGCTGGTGGCGCGCGAGCACGGCAACCTGCACCAGGTGATGTCGATGAGTGCC
GCCGCGCTGGTGCGCCTGCTCGAACGCGCCGACGCGCTGCGCAAGCCGGGGCGCTTCGCCGAGATGCTGCAGGCCTGCGA
GGCGGACGCGCGAGGCCGGCTCGGGCTCGAGACGCAGCCGTATCCGCAGGCCGAGCGGCTGCGGCGCGCGCTGGTCGCCG
CGCGCGCGGTCGATGCCGGGCAGATCGCGGCCGGCCATCGCGGCAATCCGGACGCGATCCGCGATGCCGTGCAGCGAGCA
CGCACCGACGCGGTGGCGGCGGCGCTCGCGACCGGCGAGCACGGCTGA

Upstream 100 bases:

>100_bases
TCGAGCGCATGCTCGCGCTGCCGGCGGTCGCGGCCTGGAACGAGGGCGCGCGCCGCGAGACGCGCATGGTCGAATACTGA
GAAGGCCGGGAGGCAAGCGC

Downstream 100 bases:

>100_bases
GGCTGGGAGGGTAGGACGGCGGGACGAGACGGTGGCCGGTCGGGCGCCGTGACGAGTCGGTCGGACGGCGCCTGCCGAAG
CGGCGGCGCCGGGGCGGTCG

Product: multifunctional tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase/phosphatase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MNIYVVGGAIRDALLGLPVQDRDYVVVGATPEQLAARGFRPVGKDFPVFLHPQTHEEYALARTERKTAAGYHGFQFHYAP
DVTLDEDLARRDLTINAMAREIDPDGRITGELIDPFGGRADLAAKRFRHVGPAFVEDPVRILRVARFAARFADFTVAPET
LALMREMVAAGEVDALVPERVWQEVSRGLMEAKPSRMFAVLRECGALARILPEVDALFGVPQRADYHPEVDTGVHVMMVI
DHAAKHAYSLPVRFAALTHDLGKATTPAEVLPRHIGHEGRSVDLLRPLCERLRVPNECRDLALLVAREHGNLHQVMSMSA
AALVRLLERADALRKPGRFAEMLQACEADARGRLGLETQPYPQAERLRRALVAARAVDAGQIAAGHRGNPDAIRDAVQRA
RTDAVAAALATGEHG

Sequences:

>Translated_415_residues
MNIYVVGGAIRDALLGLPVQDRDYVVVGATPEQLAARGFRPVGKDFPVFLHPQTHEEYALARTERKTAAGYHGFQFHYAP
DVTLDEDLARRDLTINAMAREIDPDGRITGELIDPFGGRADLAAKRFRHVGPAFVEDPVRILRVARFAARFADFTVAPET
LALMREMVAAGEVDALVPERVWQEVSRGLMEAKPSRMFAVLRECGALARILPEVDALFGVPQRADYHPEVDTGVHVMMVI
DHAAKHAYSLPVRFAALTHDLGKATTPAEVLPRHIGHEGRSVDLLRPLCERLRVPNECRDLALLVAREHGNLHQVMSMSA
AALVRLLERADALRKPGRFAEMLQACEADARGRLGLETQPYPQAERLRRALVAARAVDAGQIAAGHRGNPDAIRDAVQRA
RTDAVAAALATGEHG
>Mature_415_residues
MNIYVVGGAIRDALLGLPVQDRDYVVVGATPEQLAARGFRPVGKDFPVFLHPQTHEEYALARTERKTAAGYHGFQFHYAP
DVTLDEDLARRDLTINAMAREIDPDGRITGELIDPFGGRADLAAKRFRHVGPAFVEDPVRILRVARFAARFADFTVAPET
LALMREMVAAGEVDALVPERVWQEVSRGLMEAKPSRMFAVLRECGALARILPEVDALFGVPQRADYHPEVDTGVHVMMVI
DHAAKHAYSLPVRFAALTHDLGKATTPAEVLPRHIGHEGRSVDLLRPLCERLRVPNECRDLALLVAREHGNLHQVMSMSA
AALVRLLERADALRKPGRFAEMLQACEADARGRLGLETQPYPQAERLRRALVAARAVDAGQIAAGHRGNPDAIRDAVQRA
RTDAVAAALATGEHG

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=199, Percent_Identity=34.1708542713568, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI1789436, Length=410, Percent_Identity=54.1463414634146, Blast_Score=430, Evalue=1e-122,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 45545; Mature: 45545

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIYVVGGAIRDALLGLPVQDRDYVVVGATPEQLAARGFRPVGKDFPVFLHPQTHEEYAL
CEEEEECHHHHHHHHCCCCCCCCEEEECCCHHHHHHCCCCCCCCCCCEEECCCCCHHHHH
ARTERKTAAGYHGFQFHYAPDVTLDEDLARRDLTINAMAREIDPDGRITGELIDPFGGRA
HHHHHHHHCCCCCEEEEECCCCCCCHHHHHHCCHHHHHHHHCCCCCCEEHHHHCCCCCHH
DLAAKRFRHVGPAFVEDPVRILRVARFAARFADFTVAPETLALMREMVAAGEVDALVPER
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHH
VWQEVSRGLMEAKPSRMFAVLRECGALARILPEVDALFGVPQRADYHPEVDTGVHVMMVI
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEHHH
DHAAKHAYSLPVRFAALTHDLGKATTPAEVLPRHIGHEGRSVDLLRPLCERLRVPNECRD
HHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHH
LALLVAREHGNLHQVMSMSAAALVRLLERADALRKPGRFAEMLQACEADARGRLGLETQP
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCC
YPQAERLRRALVAARAVDAGQIAAGHRGNPDAIRDAVQRARTDAVAAALATGEHG
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNIYVVGGAIRDALLGLPVQDRDYVVVGATPEQLAARGFRPVGKDFPVFLHPQTHEEYAL
CEEEEECHHHHHHHHCCCCCCCCEEEECCCHHHHHHCCCCCCCCCCCEEECCCCCHHHHH
ARTERKTAAGYHGFQFHYAPDVTLDEDLARRDLTINAMAREIDPDGRITGELIDPFGGRA
HHHHHHHHCCCCCEEEEECCCCCCCHHHHHHCCHHHHHHHHCCCCCCEEHHHHCCCCCHH
DLAAKRFRHVGPAFVEDPVRILRVARFAARFADFTVAPETLALMREMVAAGEVDALVPER
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHH
VWQEVSRGLMEAKPSRMFAVLRECGALARILPEVDALFGVPQRADYHPEVDTGVHVMMVI
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEHHH
DHAAKHAYSLPVRFAALTHDLGKATTPAEVLPRHIGHEGRSVDLLRPLCERLRVPNECRD
HHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHH
LALLVAREHGNLHQVMSMSAAALVRLLERADALRKPGRFAEMLQACEADARGRLGLETQP
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCC
YPQAERLRRALVAARAVDAGQIAAGHRGNPDAIRDAVQRARTDAVAAALATGEHG
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA