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The map label for this gene is gcvA [H]

Identifier: 238028839

GI number: 238028839

Start: 3761247

End: 3762125

Strand: Direct

Name: gcvA [H]

Synonym: bglu_1g33040

Alternate gene names: 238028839

Gene position: 3761247-3762125 (Clockwise)

Preceding gene: 238028834

Following gene: 238028853

Centisome position: 96.28

GC content: 73.83

Gene sequence:

>879_bases
ATGACCCGCCTCCTGCCCCCGTTCCCGGCGCTGCGTGCCTTCGAGGCCGCCGCTCGCCATGAAAGCTTCAGCGCCGCCGG
CGACGAGCTGCATGTGACCCACGGTGCGATCAGCCGCCAGGTGGCGGCGCTCGAGGCCTGGCTCGGCCGGCCGGTGTTCC
AGCGCCACGGCAAGCGCGTGCGGCTGACCGAGGACGGGCGCCGCTATCTGGCCTCGGTGTCCGCCGCGTTCGAGCAGATC
GCCACCGCCACCGCGCGCATGCGCGACACCGCGCCCGAGCACGTGCTGCGCATCAACGCGCAGTCGACCATCGCGATGAA
ATGGCTGCTGCCGCGGCTGTCGCGGTTCCAGCGCGACATGCCCAACGTCGAGCTGAAGCTGTCCACCTCGAGCGCGCCGC
TCGACCAGCTCGACGGCCAGTTCGACGTGGCGATCCGCAGCGGCCCGGGCCACTGGCCGAACCGCGCCACCGGCCACTTC
CTCGACGAGACGCTGCTGCCGGTGTGCAGCCCGGCGCTCCTCAAGCGCATGCCGATCGCGCAGGCCGACGACCTGTCGCG
CCACGTGCTGCTGCACTCCGACACGCGGCCCACCGCGTGGCGCGACTGGTTCGCGGCGGCCGGCACGCCGCTGCGGGCCC
GCAAGAAGCAGTCGTTCGATCATTTCTACCTGGCGCTGCAGGCGGCCGTGGACGGGCTCGGCGTGGCGCTCGGCCCGCTG
CCGCTGATCGACGACGAACTCGCGGGCGGCCGGCTCGTGCTGCCGCTCGACGGCCCGCGCATCGCCTCGCGCCGCTACTG
GTGGATCGCCGCGCGCGAGGCCGCGCCGCCCGTCGTGCAGTTCTGCGCCTGGCTCGACGCGCAGGCGCGGCGCGCCTGA

Upstream 100 bases:

>100_bases
GAAATCCTTGGTGAAGTGGCCGATGCGCCGAGACTACGCCGGTCGGGGACGCCGGCAAAACGATTTTTCGGCGCGCGCCT
TGTGAGCTAAACTCACATGC

Downstream 100 bases:

>100_bases
GCGGCCGCCGGCCGCGCCGGCTCAGACCACCGGGCCCGGCTCCTTCTCCCGGCGCAGCAGCGCATAGAGCAGGATCGCGC
CGAACGTGGCGGTGCCGATC

Product: DNA-binding transcriptional activator GcvA

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MTRLLPPFPALRAFEAAARHESFSAAGDELHVTHGAISRQVAALEAWLGRPVFQRHGKRVRLTEDGRRYLASVSAAFEQI
ATATARMRDTAPEHVLRINAQSTIAMKWLLPRLSRFQRDMPNVELKLSTSSAPLDQLDGQFDVAIRSGPGHWPNRATGHF
LDETLLPVCSPALLKRMPIAQADDLSRHVLLHSDTRPTAWRDWFAAAGTPLRARKKQSFDHFYLALQAAVDGLGVALGPL
PLIDDELAGGRLVLPLDGPRIASRRYWWIAAREAAPPVVQFCAWLDAQARRA

Sequences:

>Translated_292_residues
MTRLLPPFPALRAFEAAARHESFSAAGDELHVTHGAISRQVAALEAWLGRPVFQRHGKRVRLTEDGRRYLASVSAAFEQI
ATATARMRDTAPEHVLRINAQSTIAMKWLLPRLSRFQRDMPNVELKLSTSSAPLDQLDGQFDVAIRSGPGHWPNRATGHF
LDETLLPVCSPALLKRMPIAQADDLSRHVLLHSDTRPTAWRDWFAAAGTPLRARKKQSFDHFYLALQAAVDGLGVALGPL
PLIDDELAGGRLVLPLDGPRIASRRYWWIAAREAAPPVVQFCAWLDAQARRA
>Mature_291_residues
TRLLPPFPALRAFEAAARHESFSAAGDELHVTHGAISRQVAALEAWLGRPVFQRHGKRVRLTEDGRRYLASVSAAFEQIA
TATARMRDTAPEHVLRINAQSTIAMKWLLPRLSRFQRDMPNVELKLSTSSAPLDQLDGQFDVAIRSGPGHWPNRATGHFL
DETLLPVCSPALLKRMPIAQADDLSRHVLLHSDTRPTAWRDWFAAAGTPLRARKKQSFDHFYLALQAAVDGLGVALGPLP
LIDDELAGGRLVLPLDGPRIASRRYWWIAAREAAPPVVQFCAWLDAQARRA

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=293, Percent_Identity=34.1296928327645, Blast_Score=177, Evalue=5e-46,
Organism=Escherichia coli, GI1786448, Length=253, Percent_Identity=35.1778656126482, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1788706, Length=291, Percent_Identity=28.8659793814433, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI145693193, Length=300, Percent_Identity=30.6666666666667, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1786401, Length=261, Percent_Identity=27.9693486590038, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1787128, Length=136, Percent_Identity=34.5588235294118, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI157672245, Length=160, Percent_Identity=30, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789440, Length=264, Percent_Identity=24.2424242424242, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1790208, Length=169, Percent_Identity=30.7692307692308, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1790262, Length=284, Percent_Identity=26.056338028169, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1787589, Length=256, Percent_Identity=25.78125, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI87081978, Length=255, Percent_Identity=23.1372549019608, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32418; Mature: 32286

Theoretical pI: Translated: 10.61; Mature: 10.61

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRLLPPFPALRAFEAAARHESFSAAGDELHVTHGAISRQVAALEAWLGRPVFQRHGKRV
CCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCHHHHHCCCEE
RLTEDGRRYLASVSAAFEQIATATARMRDTAPEHVLRINAQSTIAMKWLLPRLSRFQRDM
EECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEECCCHHHHHHHHHHHHHHHHHCC
PNVELKLSTSSAPLDQLDGQFDVAIRSGPGHWPNRATGHFLDETLLPVCSPALLKRMPIA
CCEEEEEECCCCCHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCHHHHHHCCCC
QADDLSRHVLLHSDTRPTAWRDWFAAAGTPLRARKKQSFDHFYLALQAAVDGLGVALGPL
CCCCCCCEEEEECCCCCCHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHCCHHCCCC
PLIDDELAGGRLVLPLDGPRIASRRYWWIAAREAAPPVVQFCAWLDAQARRA
CCCCCCCCCCEEEEECCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TRLLPPFPALRAFEAAARHESFSAAGDELHVTHGAISRQVAALEAWLGRPVFQRHGKRV
CCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCHHHHHCCCEE
RLTEDGRRYLASVSAAFEQIATATARMRDTAPEHVLRINAQSTIAMKWLLPRLSRFQRDM
EECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEECCCHHHHHHHHHHHHHHHHHCC
PNVELKLSTSSAPLDQLDGQFDVAIRSGPGHWPNRATGHFLDETLLPVCSPALLKRMPIA
CCEEEEEECCCCCHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCHHHHHHCCCC
QADDLSRHVLLHSDTRPTAWRDWFAAAGTPLRARKKQSFDHFYLALQAAVDGLGVALGPL
CCCCCCCEEEEECCCCCCHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHCCHHCCCC
PLIDDELAGGRLVLPLDGPRIASRRYWWIAAREAAPPVVQFCAWLDAQARRA
CCCCCCCCCCEEEEECCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]