Click here to switch to the map view.

The map label for this gene is amtB [H]

Identifier: 238028723

GI number: 238028723

Start: 3619257

End: 3620738

Strand: Reverse

Name: amtB [H]

Synonym: bglu_1g31870

Alternate gene names: 238028723

Gene position: 3620738-3619257 (Counterclockwise)

Preceding gene: 339905979

Following gene: 238028722

Centisome position: 92.68

GC content: 68.96

Gene sequence:

>1482_bases
ATGCGTAAACTTTTGATGTCCCTGATGATGGCCGGCTCGCTCGTCACGACCGGCATCGGCGCCGCGCGGACCGACGACGC
GTCGTCCGCGCCTGCCGTGGCTTCGGCCGCCGTCGCCTCCGACGCGTCGGCCAGCGCGCCCGCCGCGGCCCCGGCTGCCG
CCTCCGCTTCGGCCACGAGCGCCGCGGCCGCCGCACCGGCTTCGGGCGCCTCGGCCGCCGCCGCACCGGCCGCGCCGACC
GCGCCGTTCTCGGTCGATTCGTCGAAGATCAACTCGGGCGATACCGCCTGGATGCTGACCTCGGTCGCCCTCGTGCTGTT
CATGACGATTCCGGGCCTCGCGCTGTTCTACGGCGGCATGGTCCGCAAGAAGAACGTGCTGGCCACGGTGATGCAGAGCT
TCGCGATCACGGCCCTGATCACGGTGCTCTGGACCGTGGTGGGCTATAGCCTCGCGTTCACGCCGGGCAACGGCTTCATC
GGCGGCTTCTCGCGCGTGCTGCTGTCGGGCATGGCCTACGTGAAGGGCGACACGGCCACCTCGCTGACCGTCAGCCACCT
CGCCACCACGATCCCGGAATCGGTCTACTGCGTGTTCCAGATGACCTTCGCGATCATCACGCCGGCGCTGATCTGCGGCG
CGTTCGCCGATCGCATGAAGTTCTCGGCGATGCTTGTGTTCATGACGATCTGGTCGCTGATCGTCTACGTGCCGATCGCC
CACATGGTCTGGGAGCCGACCGGCTGGCTGGCCGCCGACGGCGTGCTCGACTTCGCGGGCGGCACGGTGGTCCACATCAA
CGCCGGTATCGCGGGCCTGATGGCGGCGCTCGTGCTGGGCAAGCGGGTCGGCTACGGCCGCGAATCGATGGCGCCGCACA
ACCTGGTGCTGACGCTGATCGGCGGCTCGATGCTGTGGGTGGGCTGGTTCGGCTTCAACGCGGGCTCGGCGGTGGCCGCT
GACGGCCGCGCCGGCTTCGCGATGCTGACCACGCAGATCGCCACGGCCTGCGCCGCGCTCGGCTGGATGTTCGCGGAGTG
GATCGCGAAGGGCAAGCCCTCGGTGCTCGGCATCGTCTCGGGCGCGGTGGCGGGGCTGGTGGCGATCACCCCGGCGGCCG
GCTTCGTCGGCGTGGGCGGTGCGCTCGTGATCGGCATCGCGGCGGGCGTGGTCTGCTTCTGGTCGGCCACCTGGCTCAAG
ATCAAGCTCGGATACGACGATTCGCTCGACGCGTTCGGCGTGCACGGCGTGGGCGGCATTCTCGGCGCGCTGCTGACCGG
CGTGTTCGCGGTCAAGGACATCGGCGGCTTCGACGGCAGCCTGCTGGTGCAGGCCAAGGGCGTGGCGATCACGCTGCTCT
ACAGCGGCATCGTGACCTTCATCATCCTGAAGGTGATCGACATGGTGATGGGCCTGCGCGTCTCGGAAGAGGAAGAGCGC
GAGGGTCTGGACGTGATCCTGCATGGCGAACACGTCGAATGA

Upstream 100 bases:

>100_bases
GATCTTCGTCGCGCCGATCGAACAGGTTATCCGGATTCGCACCGAGGAGACCGGCGCCGACGCCCTGTAAATCAACGACA
CGCCACACGAGGAAACCAAC

Downstream 100 bases:

>100_bases
TCGAATGACGCACCGGTAATCCGGCGGGGCGGCGCCATTCGGCCGCCCCGCCCACAGAATGAGCGCAGCGACTTCTGCCC
GCCTTTCCGGCGGGTTCTTT

Product: Ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MRKLLMSLMMAGSLVTTGIGAARTDDASSAPAVASAAVASDASASAPAAAPAAASASATSAAAAAPASGASAAAAPAAPT
APFSVDSSKINSGDTAWMLTSVALVLFMTIPGLALFYGGMVRKKNVLATVMQSFAITALITVLWTVVGYSLAFTPGNGFI
GGFSRVLLSGMAYVKGDTATSLTVSHLATTIPESVYCVFQMTFAIITPALICGAFADRMKFSAMLVFMTIWSLIVYVPIA
HMVWEPTGWLAADGVLDFAGGTVVHINAGIAGLMAALVLGKRVGYGRESMAPHNLVLTLIGGSMLWVGWFGFNAGSAVAA
DGRAGFAMLTTQIATACAALGWMFAEWIAKGKPSVLGIVSGAVAGLVAITPAAGFVGVGGALVIGIAAGVVCFWSATWLK
IKLGYDDSLDAFGVHGVGGILGALLTGVFAVKDIGGFDGSLLVQAKGVAITLLYSGIVTFIILKVIDMVMGLRVSEEEER
EGLDVILHGEHVE

Sequences:

>Translated_493_residues
MRKLLMSLMMAGSLVTTGIGAARTDDASSAPAVASAAVASDASASAPAAAPAAASASATSAAAAAPASGASAAAAPAAPT
APFSVDSSKINSGDTAWMLTSVALVLFMTIPGLALFYGGMVRKKNVLATVMQSFAITALITVLWTVVGYSLAFTPGNGFI
GGFSRVLLSGMAYVKGDTATSLTVSHLATTIPESVYCVFQMTFAIITPALICGAFADRMKFSAMLVFMTIWSLIVYVPIA
HMVWEPTGWLAADGVLDFAGGTVVHINAGIAGLMAALVLGKRVGYGRESMAPHNLVLTLIGGSMLWVGWFGFNAGSAVAA
DGRAGFAMLTTQIATACAALGWMFAEWIAKGKPSVLGIVSGAVAGLVAITPAAGFVGVGGALVIGIAAGVVCFWSATWLK
IKLGYDDSLDAFGVHGVGGILGALLTGVFAVKDIGGFDGSLLVQAKGVAITLLYSGIVTFIILKVIDMVMGLRVSEEEER
EGLDVILHGEHVE
>Mature_493_residues
MRKLLMSLMMAGSLVTTGIGAARTDDASSAPAVASAAVASDASASAPAAAPAAASASATSAAAAAPASGASAAAAPAAPT
APFSVDSSKINSGDTAWMLTSVALVLFMTIPGLALFYGGMVRKKNVLATVMQSFAITALITVLWTVVGYSLAFTPGNGFI
GGFSRVLLSGMAYVKGDTATSLTVSHLATTIPESVYCVFQMTFAIITPALICGAFADRMKFSAMLVFMTIWSLIVYVPIA
HMVWEPTGWLAADGVLDFAGGTVVHINAGIAGLMAALVLGKRVGYGRESMAPHNLVLTLIGGSMLWVGWFGFNAGSAVAA
DGRAGFAMLTTQIATACAALGWMFAEWIAKGKPSVLGIVSGAVAGLVAITPAAGFVGVGGALVIGIAAGVVCFWSATWLK
IKLGYDDSLDAFGVHGVGGILGALLTGVFAVKDIGGFDGSLLVQAKGVAITLLYSGIVTFIILKVIDMVMGLRVSEEEER
EGLDVILHGEHVE

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=409, Percent_Identity=50.6112469437653, Blast_Score=399, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17550182, Length=446, Percent_Identity=27.5784753363229, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17550180, Length=437, Percent_Identity=27.2311212814645, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI32564042, Length=434, Percent_Identity=27.6497695852535, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI133931100, Length=453, Percent_Identity=24.7240618101545, Blast_Score=94, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324187, Length=430, Percent_Identity=36.046511627907, Blast_Score=236, Evalue=8e-63,
Organism=Saccharomyces cerevisiae, GI6325396, Length=423, Percent_Identity=34.0425531914894, Blast_Score=224, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6321559, Length=414, Percent_Identity=33.5748792270531, Blast_Score=215, Evalue=1e-56,
Organism=Drosophila melanogaster, GI161078314, Length=416, Percent_Identity=27.4038461538462, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 50073; Mature: 50073

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLLMSLMMAGSLVTTGIGAARTDDASSAPAVASAAVASDASASAPAAAPAAASASATS
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCH
AAAAAPASGASAAAAPAAPTAPFSVDSSKINSGDTAWMLTSVALVLFMTIPGLALFYGGM
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCH
VRKKNVLATVMQSFAITALITVLWTVVGYSLAFTPGNGFIGGFSRVLLSGMAYVKGDTAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHCCHHCCCCCCH
SLTVSHLATTIPESVYCVFQMTFAIITPALICGAFADRMKFSAMLVFMTIWSLIVYVPIA
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HMVWEPTGWLAADGVLDFAGGTVVHINAGIAGLMAALVLGKRVGYGRESMAPHNLVLTLI
HHHCCCCCCEEECCEEECCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEE
GGSMLWVGWFGFNAGSAVAADGRAGFAMLTTQIATACAALGWMFAEWIAKGKPSVLGIVS
CCCEEEEEEECCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHH
GAVAGLVAITPAAGFVGVGGALVIGIAAGVVCFWSATWLKIKLGYDDSLDAFGVHGVGGI
HHHHHHHHHCCCCCCHHCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHH
LGALLTGVFAVKDIGGFDGSLLVQAKGVAITLLYSGIVTFIILKVIDMVMGLRVSEEEER
HHHHHHHHHHHHHCCCCCCEEEEEECCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
EGLDVILHGEHVE
CCCEEEEECCCCC
>Mature Secondary Structure
MRKLLMSLMMAGSLVTTGIGAARTDDASSAPAVASAAVASDASASAPAAAPAAASASATS
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCH
AAAAAPASGASAAAAPAAPTAPFSVDSSKINSGDTAWMLTSVALVLFMTIPGLALFYGGM
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCH
VRKKNVLATVMQSFAITALITVLWTVVGYSLAFTPGNGFIGGFSRVLLSGMAYVKGDTAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHCCHHCCCCCCH
SLTVSHLATTIPESVYCVFQMTFAIITPALICGAFADRMKFSAMLVFMTIWSLIVYVPIA
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HMVWEPTGWLAADGVLDFAGGTVVHINAGIAGLMAALVLGKRVGYGRESMAPHNLVLTLI
HHHCCCCCCEEECCEEECCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEE
GGSMLWVGWFGFNAGSAVAADGRAGFAMLTTQIATACAALGWMFAEWIAKGKPSVLGIVS
CCCEEEEEEECCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHH
GAVAGLVAITPAAGFVGVGGALVIGIAAGVVCFWSATWLKIKLGYDDSLDAFGVHGVGGI
HHHHHHHHHCCCCCCHHCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHH
LGALLTGVFAVKDIGGFDGSLLVQAKGVAITLLYSGIVTFIILKVIDMVMGLRVSEEEER
HHHHHHHHHHHHHCCCCCCEEEEEECCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
EGLDVILHGEHVE
CCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]