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The map label for this gene is mexA [H]

Identifier: 238028498

GI number: 238028498

Start: 3354557

End: 3355852

Strand: Reverse

Name: mexA [H]

Synonym: bglu_1g29620

Alternate gene names: 238028498

Gene position: 3355852-3354557 (Counterclockwise)

Preceding gene: 238028500

Following gene: 238028497

Centisome position: 85.9

GC content: 69.91

Gene sequence:

>1296_bases
ATGCGCGTCGAACGGGTTCCATACCGCTTAATCAGTGTTGCCGCGGCAGCCGTATTCCTTGCCGCTTGTGGAAAAAAACA
ACCGGCACCGCAACAGCAGATGCCCGAAGTCGGCGTCGTGACGGTGCAGCCGCAATCGGTACCGGTGATCACCGAGCTGC
CGGGGCGCACCACCGCGTTCCTCGTTGCCCAGGTGCGCGCGCGCGTGGACGGCATCGTGCTGCGCCGCGAGTTTACCGAG
GGCTCGGACGTGAAGGCCGGCCAGCGCCTCTACAAGATCGACCCGGCGCCGTACCTTGCCGCGCTCAACAGCGCCAAGGC
CACGCTCGCGAAGGCCCAGGCGAACCTGGTCTCGACCAACGCGCAGGCCGCGCGCTACAAGGTGCTGGTGGCCGCCAACG
CGGTCAGCAAGCAGGACTACGACAACGCGGTGGCCGCCGCGGGGCAGGCCGCGGCCGAGGTCGCGGCGGGCAAGGCGGCC
GTCGACACCGCGCAGATCAACCTGGGCTATACCGACGTGCTCTCGCCGATCTCGGGCCGGGTGGGGATCTCGCAGGTCAC
GCCGGGCGCCTACGTGCAGGCGAGCGCGGCGACGCTGATGTCGACCGTGCAGCAGCTCGACCCGATGTACGTCGACCTGA
CCCAGTCCAGCCTGGACGGGCTGAAGCTGCGCCAGGACATCCAGAGCGGCCGCCTGAAGACGGCGGGTCCGAACGCGGCG
AAGGTGTCGCTGATCCTGGAGGACGGCCGGACCTATTCCACCGAGGGCAAGCTGCAGTTCACCGACGTGACGGTGGATCA
GACCACGGGGTCGGTGACGATCCGCGCGATCTTCCCGAACCCCAACCGGGTGCTGCTGCCCGGCATGTTCGTGCGCGCCC
GGATCGAGGAAGGGGTGAACGACAACGCGTTCCTGGTGCCGCAGATCGGCGTGCAGCACGATCCGAAGGGCCAGCCGACG
GCGCTGGTGGTGGGGCAGGACGGCAAGGTGGCCACGCGCGTGCTGAAGACCAGCGGCACGCACGGTCAGGACTGGGTCGT
GGAAGGCGGTCTGCAGGCGGGCGACCGCGTGATCGTGCAGGGCGTGGCGAAGGTGCAGCCGGGCATGACCGTCAAGCCGG
TGGCCGCGCAGTTGCCGCCCGCGCCGGATGCGAGCGCGCCGCAAAGCGCGGCCGACAGCGGCAATGCGAACGCGAACGCG
TCGGCGGCATCGGGGGCTTCCGGCGCCTCGTCGGCGGCACCCGCGAGTGCGGCTGCCGGTTCGGCAACGAGTGCGACGGC
GGCTTCGGGCGCGTAA

Upstream 100 bases:

>100_bases
CAGTCCCGCATCTCTGGTGGGTTCGATCAGCGCCGCGAGGCGCGTTCGTGTCGTGGCTCCAGCCCGCACCCAATTCTTTT
GTTACAAACGAGGTCGCTCC

Downstream 100 bases:

>100_bases
TCACAGGGGGCCTGTTTCATGGCAAAGTTTTTTATCGATCGCCCGATCTTCGCGTGGGTGATCGCCATCATCCTGATGCT
CGCGGGTATCGCGGCGATCT

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MRVERVPYRLISVAAAAVFLAACGKKQPAPQQQMPEVGVVTVQPQSVPVITELPGRTTAFLVAQVRARVDGIVLRREFTE
GSDVKAGQRLYKIDPAPYLAALNSAKATLAKAQANLVSTNAQAARYKVLVAANAVSKQDYDNAVAAAGQAAAEVAAGKAA
VDTAQINLGYTDVLSPISGRVGISQVTPGAYVQASAATLMSTVQQLDPMYVDLTQSSLDGLKLRQDIQSGRLKTAGPNAA
KVSLILEDGRTYSTEGKLQFTDVTVDQTTGSVTIRAIFPNPNRVLLPGMFVRARIEEGVNDNAFLVPQIGVQHDPKGQPT
ALVVGQDGKVATRVLKTSGTHGQDWVVEGGLQAGDRVIVQGVAKVQPGMTVKPVAAQLPPAPDASAPQSAADSGNANANA
SAASGASGASSAAPASAAAGSATSATAASGA

Sequences:

>Translated_431_residues
MRVERVPYRLISVAAAAVFLAACGKKQPAPQQQMPEVGVVTVQPQSVPVITELPGRTTAFLVAQVRARVDGIVLRREFTE
GSDVKAGQRLYKIDPAPYLAALNSAKATLAKAQANLVSTNAQAARYKVLVAANAVSKQDYDNAVAAAGQAAAEVAAGKAA
VDTAQINLGYTDVLSPISGRVGISQVTPGAYVQASAATLMSTVQQLDPMYVDLTQSSLDGLKLRQDIQSGRLKTAGPNAA
KVSLILEDGRTYSTEGKLQFTDVTVDQTTGSVTIRAIFPNPNRVLLPGMFVRARIEEGVNDNAFLVPQIGVQHDPKGQPT
ALVVGQDGKVATRVLKTSGTHGQDWVVEGGLQAGDRVIVQGVAKVQPGMTVKPVAAQLPPAPDASAPQSAADSGNANANA
SAASGASGASSAAPASAAAGSATSATAASGA
>Mature_431_residues
MRVERVPYRLISVAAAAVFLAACGKKQPAPQQQMPEVGVVTVQPQSVPVITELPGRTTAFLVAQVRARVDGIVLRREFTE
GSDVKAGQRLYKIDPAPYLAALNSAKATLAKAQANLVSTNAQAARYKVLVAANAVSKQDYDNAVAAAGQAAAEVAAGKAA
VDTAQINLGYTDVLSPISGRVGISQVTPGAYVQASAATLMSTVQQLDPMYVDLTQSSLDGLKLRQDIQSGRLKTAGPNAA
KVSLILEDGRTYSTEGKLQFTDVTVDQTTGSVTIRAIFPNPNRVLLPGMFVRARIEEGVNDNAFLVPQIGVQHDPKGQPT
ALVVGQDGKVATRVLKTSGTHGQDWVVEGGLQAGDRVIVQGVAKVQPGMTVKPVAAQLPPAPDASAPQSAADSGNANANA
SAASGASGASSAAPASAAAGSATSATAASGA

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=368, Percent_Identity=55.1630434782609, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI1789929, Length=368, Percent_Identity=48.0978260869565, Blast_Score=328, Evalue=4e-91,
Organism=Escherichia coli, GI1789665, Length=361, Percent_Identity=49.0304709141274, Blast_Score=328, Evalue=4e-91,
Organism=Escherichia coli, GI87082036, Length=346, Percent_Identity=31.2138728323699, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI87081796, Length=332, Percent_Identity=26.5060240963855, Blast_Score=62, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 44094; Mature: 44094

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVERVPYRLISVAAAAVFLAACGKKQPAPQQQMPEVGVVTVQPQSVPVITELPGRTTAF
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCEEEECCCCCCEEEECCCCHHHH
LVAQVRARVDGIVLRREFTEGSDVKAGQRLYKIDPAPYLAALNSAKATLAKAQANLVSTN
HHHHHHHHHCEEEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCC
AQAARYKVLVAANAVSKQDYDNAVAAAGQAAAEVAAGKAAVDTAQINLGYTDVLSPISGR
CCHHEEEEEEEECCCCCCCCCHHHHHCCHHHHHHHCCCCEEEEEEEECCHHHHHHCCCCC
VGISQVTPGAYVQASAATLMSTVQQLDPMYVDLTQSSLDGLKLRQDIQSGRLKTAGPNAA
CCCEECCCCCEEEHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCEECCCCCCE
KVSLILEDGRTYSTEGKLQFTDVTVDQTTGSVTIRAIFPNPNRVLLPGMFVRARIEEGVN
EEEEEEECCCEECCCCEEEEEEEEEECCCCCEEEEEECCCCCEEEECCHHHHHHHHCCCC
DNAFLVPQIGVQHDPKGQPTALVVGQDGKVATRVLKTSGTHGQDWVVEGGLQAGDRVIVQ
CCEEEEEECCCCCCCCCCCEEEEEECCCCHHHHHHHCCCCCCCCEEEECCCCCCCEEEEE
GVAKVQPGMTVKPVAAQLPPAPDASAPQSAADSGNANANASAASGASGASSAAPASAAAG
HHHHCCCCCEECCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SATSATAASGA
CCCCCCCCCCC
>Mature Secondary Structure
MRVERVPYRLISVAAAAVFLAACGKKQPAPQQQMPEVGVVTVQPQSVPVITELPGRTTAF
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCEEEECCCCCCEEEECCCCHHHH
LVAQVRARVDGIVLRREFTEGSDVKAGQRLYKIDPAPYLAALNSAKATLAKAQANLVSTN
HHHHHHHHHCEEEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCC
AQAARYKVLVAANAVSKQDYDNAVAAAGQAAAEVAAGKAAVDTAQINLGYTDVLSPISGR
CCHHEEEEEEEECCCCCCCCCHHHHHCCHHHHHHHCCCCEEEEEEEECCHHHHHHCCCCC
VGISQVTPGAYVQASAATLMSTVQQLDPMYVDLTQSSLDGLKLRQDIQSGRLKTAGPNAA
CCCEECCCCCEEEHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCEECCCCCCE
KVSLILEDGRTYSTEGKLQFTDVTVDQTTGSVTIRAIFPNPNRVLLPGMFVRARIEEGVN
EEEEEEECCCEECCCCEEEEEEEEEECCCCCEEEEEECCCCCEEEECCHHHHHHHHCCCC
DNAFLVPQIGVQHDPKGQPTALVVGQDGKVATRVLKTSGTHGQDWVVEGGLQAGDRVIVQ
CCEEEEEECCCCCCCCCCCEEEEEECCCCHHHHHHHCCCCCCCCEEEECCCCCCCEEEEE
GVAKVQPGMTVKPVAAQLPPAPDASAPQSAADSGNANANASAASGASGASSAAPASAAAG
HHHHCCCCCEECCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SATSATAASGA
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]