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The map label for this gene is oprM [H]

Identifier: 238028496

GI number: 238028496

Start: 3349827

End: 3351344

Strand: Reverse

Name: oprM [H]

Synonym: bglu_1g29600

Alternate gene names: 238028496

Gene position: 3351344-3349827 (Counterclockwise)

Preceding gene: 238028497

Following gene: 238028494

Centisome position: 85.79

GC content: 71.08

Gene sequence:

>1518_bases
ATGCGAAAATTTGCTTTGACTGCAATGGCGGTCGCGCTGGTCGCCACCGGCTGCACGCTGGCCCCGCACTACGAGCGTCC
GGCCGCGCCGGTCGCGGGTACGTTCCCGTCCGACGGCGTCTACGCGACGCAGCCGGGCGCGAGCGCGACCCGCAGCGCGA
ACGGCCAGGCCGCGAGCGACATCGGCTGGCGCAACTTCTTCACCGATCCGCGGCTGCAGCGGCTGATCGAGCTGGCGCTG
AAGAACAACCGCGACCTGCGCGTATCGGTGCTCAACGTGGAGGCGGCGCGCGCGCAGTATCAGATCACCCGCGCCGAGCT
GCTGCCGGCCCTGAACGGCACCGGCACCGGCTCGATCCAGCGGCTGCCGGCCGGAGTCTCGACCACGGGGGCGCCGCTCA
TCTCGCGCACCTACAACGTCGGGCTCTCGGCGTCGTGGGAGCTGGACCTGTTCGGCCGGATCCAGAGCCTGAAGGATCAG
GCGCTCGCGAAATACCTGTCCACGGCGCAGGCGCGCAAGGCCGCGGAGATATCGCTCGTCTCGCAGGTGGCGGACCAGTA
TCTGACGGTGCTGTCCACCGACGACCTGCTGAAGGTGACGGAAGACACGCTGAAGACGGCGCAGGCGTCGTACAACCTGA
CCAAGCTGCAGTTCGACAACGGCACGGGCTCGGAGCTGGATCTGCGTCAGGCGCAGACGGTGGTCGAGCAGGCGCTGGCG
AACCAGCAGGCGCAGGCGCGGGCGCGGGCCCAGGCGCTCAACGCGCTGGTGGTGCTGGTGGGCGAGCCGCTGCCGGACGA
CCTGCCGGCCGGGCAGCCGCTCGACTCGCAGGACCTGCTGACGGACATCCCGGCCGGATTGCCGTCGGAGCTGCTCACGC
GCCGGCCCGACATCATGGAGGCCGAGCAGACGCTGCGCGCGGCCAACGCGAACATCGGCGCGGCGCGCGCGGCGTTCTTC
CCGCGCATCTCGCTGACGGGCGCGTTCGGCTCGGCGAGCCCGACGCTGGGCGGGCTGCTCAAGGCGGGGACGGCGGCCTG
GTCGTTCGCGCCGGACCTCACGCTGCCGATCTTCGCGGGCGGCTCGAACCTGGCGAACCTGGACCTGGCGCGCGTGCAGA
AGCGCATCGAGGTGGCGAACTACGAGAAGGCGATCCAGACGGCGTTCCAGGAGGTGTCGGACGGTCTGGCCGCGCGCGGG
ACGTACGACCAGCAGATCGCGGCGCTGCAGCGCAACGAGCAGGCGCAGCGGCGCCGCTACGACCTGTCGGAGCTGCGCTA
CAAGAACGGGGTCGACAACTACCTGACGGTGCTGACGGCGCAGACGGACCTGTATTCGGCGCAGCAGTCGCTGATCTCGG
CACGGCTGTCGCGCTGGACCAACCTGGTGGACCTGTACCGCGCGCTGGGCGGCGGCTGGCTCGAGCACTCGGGCGACACG
CCGCGCGAGGCGGATGCGCCGGCCGACTACGGCAAGGCCGCGCCTGCGGTGCCGGCTTCGCAGGCCACGGCGGGCTGA

Upstream 100 bases:

>100_bases
TCGGCGGCGAGAAGGAAGACGCCGACGAGGCGCTGCGTCTGGCGCAGGAGCACGCGCACCGGACCCAGGAAGGCGGCGAC
GGCAACAAGGAACAGTGACA

Downstream 100 bases:

>100_bases
GCCCGGCCGAATCCCGCGCGCGGCACGGCAGCACGTCACGACACCCCGGCAGGCTTCACGGCCTGCCGGGGTGTCGTTTT
TCTGCGCCGCAACGCGCCAC

Product: RND efflux system outer membrane lipoprotein NodT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MRKFALTAMAVALVATGCTLAPHYERPAAPVAGTFPSDGVYATQPGASATRSANGQAASDIGWRNFFTDPRLQRLIELAL
KNNRDLRVSVLNVEAARAQYQITRAELLPALNGTGTGSIQRLPAGVSTTGAPLISRTYNVGLSASWELDLFGRIQSLKDQ
ALAKYLSTAQARKAAEISLVSQVADQYLTVLSTDDLLKVTEDTLKTAQASYNLTKLQFDNGTGSELDLRQAQTVVEQALA
NQQAQARARAQALNALVVLVGEPLPDDLPAGQPLDSQDLLTDIPAGLPSELLTRRPDIMEAEQTLRAANANIGAARAAFF
PRISLTGAFGSASPTLGGLLKAGTAAWSFAPDLTLPIFAGGSNLANLDLARVQKRIEVANYEKAIQTAFQEVSDGLAARG
TYDQQIAALQRNEQAQRRRYDLSELRYKNGVDNYLTVLTAQTDLYSAQQSLISARLSRWTNLVDLYRALGGGWLEHSGDT
PREADAPADYGKAAPAVPASQATAG

Sequences:

>Translated_505_residues
MRKFALTAMAVALVATGCTLAPHYERPAAPVAGTFPSDGVYATQPGASATRSANGQAASDIGWRNFFTDPRLQRLIELAL
KNNRDLRVSVLNVEAARAQYQITRAELLPALNGTGTGSIQRLPAGVSTTGAPLISRTYNVGLSASWELDLFGRIQSLKDQ
ALAKYLSTAQARKAAEISLVSQVADQYLTVLSTDDLLKVTEDTLKTAQASYNLTKLQFDNGTGSELDLRQAQTVVEQALA
NQQAQARARAQALNALVVLVGEPLPDDLPAGQPLDSQDLLTDIPAGLPSELLTRRPDIMEAEQTLRAANANIGAARAAFF
PRISLTGAFGSASPTLGGLLKAGTAAWSFAPDLTLPIFAGGSNLANLDLARVQKRIEVANYEKAIQTAFQEVSDGLAARG
TYDQQIAALQRNEQAQRRRYDLSELRYKNGVDNYLTVLTAQTDLYSAQQSLISARLSRWTNLVDLYRALGGGWLEHSGDT
PREADAPADYGKAAPAVPASQATAG
>Mature_505_residues
MRKFALTAMAVALVATGCTLAPHYERPAAPVAGTFPSDGVYATQPGASATRSANGQAASDIGWRNFFTDPRLQRLIELAL
KNNRDLRVSVLNVEAARAQYQITRAELLPALNGTGTGSIQRLPAGVSTTGAPLISRTYNVGLSASWELDLFGRIQSLKDQ
ALAKYLSTAQARKAAEISLVSQVADQYLTVLSTDDLLKVTEDTLKTAQASYNLTKLQFDNGTGSELDLRQAQTVVEQALA
NQQAQARARAQALNALVVLVGEPLPDDLPAGQPLDSQDLLTDIPAGLPSELLTRRPDIMEAEQTLRAANANIGAARAAFF
PRISLTGAFGSASPTLGGLLKAGTAAWSFAPDLTLPIFAGGSNLANLDLARVQKRIEVANYEKAIQTAFQEVSDGLAARG
TYDQQIAALQRNEQAQRRRYDLSELRYKNGVDNYLTVLTAQTDLYSAQQSLISARLSRWTNLVDLYRALGGGWLEHSGDT
PREADAPADYGKAAPAVPASQATAG

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=474, Percent_Identity=41.7721518987342, Blast_Score=328, Evalue=4e-91,
Organism=Escherichia coli, GI2367348, Length=425, Percent_Identity=25.1764705882353, Blast_Score=102, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 53925; Mature: 53925

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKFALTAMAVALVATGCTLAPHYERPAAPVAGTFPSDGVYATQPGASATRSANGQAASD
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCHHC
IGWRNFFTDPRLQRLIELALKNNRDLRVSVLNVEAARAQYQITRAELLPALNGTGTGSIQ
CCCHHCCCCHHHHHHHHHHHCCCCCEEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCHH
RLPAGVSTTGAPLISRTYNVGLSASWELDLFGRIQSLKDQALAKYLSTAQARKAAEISLV
HCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SQVADQYLTVLSTDDLLKVTEDTLKTAQASYNLTKLQFDNGTGSELDLRQAQTVVEQALA
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHH
NQQAQARARAQALNALVVLVGEPLPDDLPAGQPLDSQDLLTDIPAGLPSELLTRRPDIME
HHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHCCCCHHH
AEQTLRAANANIGAARAAFFPRISLTGAFGSASPTLGGLLKAGTAAWSFAPDLTLPIFAG
HHHHHHHHCCCCCHHHHHHCCCEEEEECCCCCCCHHHHHHHHCCCCEECCCCCCEEEEEC
GSNLANLDLARVQKRIEVANYEKAIQTAFQEVSDGLAARGTYDQQIAALQRNEQAQRRRY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHC
DLSELRYKNGVDNYLTVLTAQTDLYSAQQSLISARLSRWTNLVDLYRALGGGWLEHSGDT
CHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PREADAPADYGKAAPAVPASQATAG
CCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRKFALTAMAVALVATGCTLAPHYERPAAPVAGTFPSDGVYATQPGASATRSANGQAASD
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCHHC
IGWRNFFTDPRLQRLIELALKNNRDLRVSVLNVEAARAQYQITRAELLPALNGTGTGSIQ
CCCHHCCCCHHHHHHHHHHHCCCCCEEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCHH
RLPAGVSTTGAPLISRTYNVGLSASWELDLFGRIQSLKDQALAKYLSTAQARKAAEISLV
HCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SQVADQYLTVLSTDDLLKVTEDTLKTAQASYNLTKLQFDNGTGSELDLRQAQTVVEQALA
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHH
NQQAQARARAQALNALVVLVGEPLPDDLPAGQPLDSQDLLTDIPAGLPSELLTRRPDIME
HHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHCCCCHHH
AEQTLRAANANIGAARAAFFPRISLTGAFGSASPTLGGLLKAGTAAWSFAPDLTLPIFAG
HHHHHHHHCCCCCHHHHHHCCCEEEEECCCCCCCHHHHHHHHCCCCEECCCCCCEEEEEC
GSNLANLDLARVQKRIEVANYEKAIQTAFQEVSDGLAARGTYDQQIAALQRNEQAQRRRY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHC
DLSELRYKNGVDNYLTVLTAQTDLYSAQQSLISARLSRWTNLVDLYRALGGGWLEHSGDT
CHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PREADAPADYGKAAPAVPASQATAG
CCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]