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The map label for this gene is braE [H]

Identifier: 238028399

GI number: 238028399

Start: 3243937

End: 3245109

Strand: Reverse

Name: braE [H]

Synonym: bglu_1g28630

Alternate gene names: 238028399

Gene position: 3245109-3243937 (Counterclockwise)

Preceding gene: 238028400

Following gene: 238028398

Centisome position: 83.07

GC content: 65.9

Gene sequence:

>1173_bases
ATGACTTCCATTCAACCGGTCGAAACCTCGACGACGCTCATCGAAGAGCGCAATACCGCGAAGACGCTCGCCATCGGCGT
AGTGGTCGCGCTGCTGGTGGCGGCGGCCCCGTTCCTGATCGGCGCGGCCGGCGGCAACTACTGGGTCCGCGTGCTCGATT
TCGCGATGCTGTACGTGATGCTGGCGCTGGGCCTGAACGTGGTGGTCGGTTTCGCCGGCCTGCTCGACCTCGGCTACATC
GCGTTCTACGCGGTGGGCGCCTACGTCGCCGCGCTGCTGACCTCGCCGCACCTGACCACGCAGTTCGCCTGGATCGCCGC
GATGTTCCCGAACGGCCTGCACACGCCGTACTGGGTGGTGGTGCCGGTCGCGATGGCGCTCGCCGCGCTCGCGGGCGTGC
TGCTCGGCGCACCGACGCTGCGCCTGCGCGGCGACTACCTGGCGATCGTCACGCTGGGCTTCGGGGAAATCGTCCGGATC
TTCATGAACAACCTGGACCGTCCGATCAACATCACCAACGGGCCGCAAGGGATCACGGGCGTCGCGCCGCTGCACTTCGG
CAGTTTCAACCTGTCGCAGACGCACACGCTGTTCGGCTTCAGCATCACCTCGGTCTACCTGTACTACTACGTGTTCGTGA
TCTGCTCGCTGCTGGTGATCTGGGTCTGCACGCGCTTGCAGCATTCGCGCATCGGCCGTGCCTGGGCCGCGATCCGCGAG
GACGAGATCGCCGCCAAGGCGATGGGCATCAACACGCGCAACGTCAAGCTGCTCGCGTTCGCGATGGGCGCCTCGTTCGG
CGGCCTGTCGGGTTCGATGTTCGGCGCGTTCCAGGGCTTCGTGTCGCCGGAATCGTTCACCTTCTGGGAATCGATCGTGG
TGCTGGCCTGCGTGGTGCTGGGCGGCATGGGCCATATTCCCGGCGTGATCCTCGGCGCGGTGCTGCTGGCCGTGTTCCCG
GAAGTGCTGCGCTCGACCATGGGTCCGCTGCAGCATGCGCTGTTCGGCCACGAGATCGTCGACACGGAAGTGATCCGCCA
GGCGCTCTACGGCCTCGCGATGGTGCTGATCATGCTGTACCGCTCGGAAGGCCTATGGCCGGCGCCGAAGCACGAGGACA
AGATCGCCAGGATCTCGAAGCGCAACGGCAAGAAGACGGCGGTTCGCGCGTAA

Upstream 100 bases:

>100_bases
CAGGACGTGTTCGCGTTCGTCGTGCTGATCGTGGTGCTGGTGTTCCGTCCGTCCGGCCTGCTGGGCGAGCGTGTGGCCGA
TCGCGCATAAGGAGACCAAC

Downstream 100 bases:

>100_bases
CGGACGGATACGGAGAAACAAGCAATGAGCGAGAACATTCGACTGTCCGTGAAGGGCGTGAACAAGCGCTTCGGCGGCCT
GCAGGCGCTGTCGGACGTCG

Product: Inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MTSIQPVETSTTLIEERNTAKTLAIGVVVALLVAAAPFLIGAAGGNYWVRVLDFAMLYVMLALGLNVVVGFAGLLDLGYI
AFYAVGAYVAALLTSPHLTTQFAWIAAMFPNGLHTPYWVVVPVAMALAALAGVLLGAPTLRLRGDYLAIVTLGFGEIVRI
FMNNLDRPINITNGPQGITGVAPLHFGSFNLSQTHTLFGFSITSVYLYYYVFVICSLLVIWVCTRLQHSRIGRAWAAIRE
DEIAAKAMGINTRNVKLLAFAMGASFGGLSGSMFGAFQGFVSPESFTFWESIVVLACVVLGGMGHIPGVILGAVLLAVFP
EVLRSTMGPLQHALFGHEIVDTEVIRQALYGLAMVLIMLYRSEGLWPAPKHEDKIARISKRNGKKTAVRA

Sequences:

>Translated_390_residues
MTSIQPVETSTTLIEERNTAKTLAIGVVVALLVAAAPFLIGAAGGNYWVRVLDFAMLYVMLALGLNVVVGFAGLLDLGYI
AFYAVGAYVAALLTSPHLTTQFAWIAAMFPNGLHTPYWVVVPVAMALAALAGVLLGAPTLRLRGDYLAIVTLGFGEIVRI
FMNNLDRPINITNGPQGITGVAPLHFGSFNLSQTHTLFGFSITSVYLYYYVFVICSLLVIWVCTRLQHSRIGRAWAAIRE
DEIAAKAMGINTRNVKLLAFAMGASFGGLSGSMFGAFQGFVSPESFTFWESIVVLACVVLGGMGHIPGVILGAVLLAVFP
EVLRSTMGPLQHALFGHEIVDTEVIRQALYGLAMVLIMLYRSEGLWPAPKHEDKIARISKRNGKKTAVRA
>Mature_389_residues
TSIQPVETSTTLIEERNTAKTLAIGVVVALLVAAAPFLIGAAGGNYWVRVLDFAMLYVMLALGLNVVVGFAGLLDLGYIA
FYAVGAYVAALLTSPHLTTQFAWIAAMFPNGLHTPYWVVVPVAMALAALAGVLLGAPTLRLRGDYLAIVTLGFGEIVRIF
MNNLDRPINITNGPQGITGVAPLHFGSFNLSQTHTLFGFSITSVYLYYYVFVICSLLVIWVCTRLQHSRIGRAWAAIRED
EIAAKAMGINTRNVKLLAFAMGASFGGLSGSMFGAFQGFVSPESFTFWESIVVLACVVLGGMGHIPGVILGAVLLAVFPE
VLRSTMGPLQHALFGHEIVDTEVIRQALYGLAMVLIMLYRSEGLWPAPKHEDKIARISKRNGKKTAVRA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=356, Percent_Identity=42.6966292134831, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1789866, Length=320, Percent_Identity=25.3125, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 42010; Mature: 41879

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSIQPVETSTTLIEERNTAKTLAIGVVVALLVAAAPFLIGAAGGNYWVRVLDFAMLYVM
CCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHH
LALGLNVVVGFAGLLDLGYIAFYAVGAYVAALLTSPHLTTQFAWIAAMFPNGLHTPYWVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHH
VPVAMALAALAGVLLGAPTLRLRGDYLAIVTLGFGEIVRIFMNNLDRPINITNGPQGITG
HHHHHHHHHHHHHHHCCCCEEECCCEEEEEECCHHHHHHHHHHCCCCCEECCCCCCCCCC
VAPLHFGSFNLSQTHTLFGFSITSVYLYYYVFVICSLLVIWVCTRLQHSRIGRAWAAIRE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEIAAKAMGINTRNVKLLAFAMGASFGGLSGSMFGAFQGFVSPESFTFWESIVVLACVVL
HHHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
GGMGHIPGVILGAVLLAVFPEVLRSTMGPLQHALFGHEIVDTEVIRQALYGLAMVLIMLY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
RSEGLWPAPKHEDKIARISKRNGKKTAVRA
HCCCCCCCCCCHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TSIQPVETSTTLIEERNTAKTLAIGVVVALLVAAAPFLIGAAGGNYWVRVLDFAMLYVM
CCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHH
LALGLNVVVGFAGLLDLGYIAFYAVGAYVAALLTSPHLTTQFAWIAAMFPNGLHTPYWVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHH
VPVAMALAALAGVLLGAPTLRLRGDYLAIVTLGFGEIVRIFMNNLDRPINITNGPQGITG
HHHHHHHHHHHHHHHCCCCEEECCCEEEEEECCHHHHHHHHHHCCCCCEECCCCCCCCCC
VAPLHFGSFNLSQTHTLFGFSITSVYLYYYVFVICSLLVIWVCTRLQHSRIGRAWAAIRE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEIAAKAMGINTRNVKLLAFAMGASFGGLSGSMFGAFQGFVSPESFTFWESIVVLACVVL
HHHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
GGMGHIPGVILGAVLLAVFPEVLRSTMGPLQHALFGHEIVDTEVIRQALYGLAMVLIMLY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
RSEGLWPAPKHEDKIARISKRNGKKTAVRA
HCCCCCCCCCCHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]