Click here to switch to the map view.

The map label for this gene is puuA [H]

Identifier: 238028060

GI number: 238028060

Start: 2856214

End: 2857548

Strand: Reverse

Name: puuA [H]

Synonym: bglu_1g25110

Alternate gene names: 238028060

Gene position: 2857548-2856214 (Counterclockwise)

Preceding gene: 238028061

Following gene: 238028059

Centisome position: 73.15

GC content: 66.44

Gene sequence:

>1335_bases
ATGCAAGACCTTGACGATTTCATGAAGCAGCACCGGATCACCGAAGTCGAGGCGATCATTCCCGACATGGCCGGCATTGC
GCGCGGCAAGATCATCCCGCGCAACAAGTTCGAGACGGGCGAATCGATGCGCCTGCCGCAGGCGGTGATGGTGCAGACCG
TCACCGGCGACTATCCCGAGGATGGCTCGCTGTCCGGCGTGACCGATCCCGACATGGTCTGCGTGCCGGACCCGTCCACC
ATCTGCCTGATCCCGTGGGCCGTCGATCCGACCGCCCAGGTGATCCACGACTGCGTCCATTTCGACGGCTCGCCGGTGGC
GATCTCGCCGCGCTACGTGCTGCGGCGCGTGCTGGAACTGTACCGCCAGCGGGGCTGGCGGCCGGTGGTGGCGCCCGAGC
TCGAGTTCTACCTGGTGGAGATGAACAAGGACCCCGACCTGCCGCTGCGCCCGCCGGTGGGCCGCACCGGGCGCGCGGAG
ACGGGCCGCCAGTCGTACTCGATCGAGGCCGTCAACGAATTCGATCCGCTGTTCGAGGACATCTACGAATACTGCGACAT
GCAGGGCCTCGACATCGACACGCTGATCCACGAGGTCGGCGCCGCGCAGATGGAGATCAACTTCCAGCACGGCGATGCGC
TCTCGCTGGCCGACCAGGTGTTCCTGTTCAAGCGCACCGTGCGCGAGGCCGCGCTGCGCCACAACATGTACGCGACGTTC
ATGGCCAAGCCGATGGAGAACGAGCCCGGCTCGGCGATGCACATCCACCAGAGCATCGTCGACCTCGAAGACGGGCGCAA
CCTGTTCGTCGACGCACAGGGCGAATCGACCGACATGTTCCGCAGCTATCTGGCGGGCCTGCAGAAATACACGCCGGCGC
TGATGCCGATCTTCGCGCCGTACATCAACTCGTACCGGCGGCTGTCGCGCTTCATGGCCGCGCCGATCAACGTGCAGTGG
GGCTACGACAACCGCACGGTCGGCTTCCGGATCCCGCACGCGGCGCCGGCCGCACGGCGCATCGAGAACCGCATCCCCGG
CGTGGACTGCAACCCGTACCTGGCACTGGCCGGCACGCTCGCGGCCGGCTATCTGGGCATCACGCAGCGCCTGAATCCGA
CCGCGCCGCTCGCGGCCGACGGCTACAGCCTGCCGTACCAGCTGCCGCGCAACCTGGAGGAGGGGCTCACGCTGATGGCC
GCTTCCACGCCGCTTGCCGAGATTCTCGGCGAGAAGTTCGTGAAGGCGTATCTGGCGCTGAAGGAAACCGAGTACGAGGC
GTTCTTCCGCGTGATCAGCTCGTGGGAGCGGCGCCATCTGCTGCTGCACGTGTGA

Upstream 100 bases:

>100_bases
GGCGTTCGGCGAGGCCTGCCGAGCCCGCCGGCGCAAAGCAGCCGAAGCGGCCTAGGCGGCCGAGCCCGCGGGCGCGGGCA
ACCATCACAACGAGGCTGAC

Downstream 100 bases:

>100_bases
CGAAACCCGAACCCGCGGCGCTCGCGCCGCCCCACGGAGGACTCATCATGACGCAACGAATCGGCGAATCGGCCTACGTC
CAGCCGGTGCGCGCACCGGC

Product: putative glutamine synthetase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MQDLDDFMKQHRITEVEAIIPDMAGIARGKIIPRNKFETGESMRLPQAVMVQTVTGDYPEDGSLSGVTDPDMVCVPDPST
ICLIPWAVDPTAQVIHDCVHFDGSPVAISPRYVLRRVLELYRQRGWRPVVAPELEFYLVEMNKDPDLPLRPPVGRTGRAE
TGRQSYSIEAVNEFDPLFEDIYEYCDMQGLDIDTLIHEVGAAQMEINFQHGDALSLADQVFLFKRTVREAALRHNMYATF
MAKPMENEPGSAMHIHQSIVDLEDGRNLFVDAQGESTDMFRSYLAGLQKYTPALMPIFAPYINSYRRLSRFMAAPINVQW
GYDNRTVGFRIPHAAPAARRIENRIPGVDCNPYLALAGTLAAGYLGITQRLNPTAPLAADGYSLPYQLPRNLEEGLTLMA
ASTPLAEILGEKFVKAYLALKETEYEAFFRVISSWERRHLLLHV

Sequences:

>Translated_444_residues
MQDLDDFMKQHRITEVEAIIPDMAGIARGKIIPRNKFETGESMRLPQAVMVQTVTGDYPEDGSLSGVTDPDMVCVPDPST
ICLIPWAVDPTAQVIHDCVHFDGSPVAISPRYVLRRVLELYRQRGWRPVVAPELEFYLVEMNKDPDLPLRPPVGRTGRAE
TGRQSYSIEAVNEFDPLFEDIYEYCDMQGLDIDTLIHEVGAAQMEINFQHGDALSLADQVFLFKRTVREAALRHNMYATF
MAKPMENEPGSAMHIHQSIVDLEDGRNLFVDAQGESTDMFRSYLAGLQKYTPALMPIFAPYINSYRRLSRFMAAPINVQW
GYDNRTVGFRIPHAAPAARRIENRIPGVDCNPYLALAGTLAAGYLGITQRLNPTAPLAADGYSLPYQLPRNLEEGLTLMA
ASTPLAEILGEKFVKAYLALKETEYEAFFRVISSWERRHLLLHV
>Mature_444_residues
MQDLDDFMKQHRITEVEAIIPDMAGIARGKIIPRNKFETGESMRLPQAVMVQTVTGDYPEDGSLSGVTDPDMVCVPDPST
ICLIPWAVDPTAQVIHDCVHFDGSPVAISPRYVLRRVLELYRQRGWRPVVAPELEFYLVEMNKDPDLPLRPPVGRTGRAE
TGRQSYSIEAVNEFDPLFEDIYEYCDMQGLDIDTLIHEVGAAQMEINFQHGDALSLADQVFLFKRTVREAALRHNMYATF
MAKPMENEPGSAMHIHQSIVDLEDGRNLFVDAQGESTDMFRSYLAGLQKYTPALMPIFAPYINSYRRLSRFMAAPINVQW
GYDNRTVGFRIPHAAPAARRIENRIPGVDCNPYLALAGTLAAGYLGITQRLNPTAPLAADGYSLPYQLPRNLEEGLTLMA
ASTPLAEILGEKFVKAYLALKETEYEAFFRVISSWERRHLLLHV

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=441, Percent_Identity=25.8503401360544, Blast_Score=149, Evalue=5e-36,
Organism=Escherichia coli, GI87081870, Length=450, Percent_Identity=35.1111111111111, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1790301, Length=406, Percent_Identity=28.3251231527094, Blast_Score=129, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 49997; Mature: 49997

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDLDDFMKQHRITEVEAIIPDMAGIARGKIIPRNKFETGESMRLPQAVMVQTVTGDYPE
CCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHEEEECCCCCCC
DGSLSGVTDPDMVCVPDPSTICLIPWAVDPTAQVIHDCVHFDGSPVAISPRYVLRRVLEL
CCCCCCCCCCCEEEECCCCEEEEEEECCCCHHHHHHHHHHCCCCCEEECHHHHHHHHHHH
YRQRGWRPVVAPELEFYLVEMNKDPDLPLRPPVGRTGRAETGRQSYSIEAVNEFDPLFED
HHHCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHH
IYEYCDMQGLDIDTLIHEVGAAQMEINFQHGDALSLADQVFLFKRTVREAALRHNMYATF
HHHHHCCCCCCHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHE
MAKPMENEPGSAMHIHQSIVDLEDGRNLFVDAQGESTDMFRSYLAGLQKYTPALMPIFAP
EECCCCCCCCCEEEHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCHHHHHHHHH
YINSYRRLSRFMAAPINVQWGYDNRTVGFRIPHAAPAARRIENRIPGVDCNPYLALAGTL
HHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCCCHHHHHHHHCCCCCCCCCHHHHHHHH
AAGYLGITQRLNPTAPLAADGYSLPYQLPRNLEEGLTLMAASTPLAEILGEKFVKAYLAL
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHCCEEEECCCCHHHHHHHHHHHHHHHH
KETEYEAFFRVISSWERRHLLLHV
HHHHHHHHHHHHHHHHHCEEEEEC
>Mature Secondary Structure
MQDLDDFMKQHRITEVEAIIPDMAGIARGKIIPRNKFETGESMRLPQAVMVQTVTGDYPE
CCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHEEEECCCCCCC
DGSLSGVTDPDMVCVPDPSTICLIPWAVDPTAQVIHDCVHFDGSPVAISPRYVLRRVLEL
CCCCCCCCCCCEEEECCCCEEEEEEECCCCHHHHHHHHHHCCCCCEEECHHHHHHHHHHH
YRQRGWRPVVAPELEFYLVEMNKDPDLPLRPPVGRTGRAETGRQSYSIEAVNEFDPLFED
HHHCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHH
IYEYCDMQGLDIDTLIHEVGAAQMEINFQHGDALSLADQVFLFKRTVREAALRHNMYATF
HHHHHCCCCCCHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHE
MAKPMENEPGSAMHIHQSIVDLEDGRNLFVDAQGESTDMFRSYLAGLQKYTPALMPIFAP
EECCCCCCCCCEEEHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCHHHHHHHHH
YINSYRRLSRFMAAPINVQWGYDNRTVGFRIPHAAPAARRIENRIPGVDCNPYLALAGTL
HHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCCCHHHHHHHHCCCCCCCCCHHHHHHHH
AAGYLGITQRLNPTAPLAADGYSLPYQLPRNLEEGLTLMAASTPLAEILGEKFVKAYLAL
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHCCEEEECCCCHHHHHHHHHHHHHHHH
KETEYEAFFRVISSWERRHLLLHV
HHHHHHHHHHHHHHHHHCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]