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The map label for this gene is ntrB [H]

Identifier: 238028048

GI number: 238028048

Start: 2839755

End: 2840897

Strand: Reverse

Name: ntrB [H]

Synonym: bglu_1g24990

Alternate gene names: 238028048

Gene position: 2840897-2839755 (Counterclockwise)

Preceding gene: 238028049

Following gene: 238028047

Centisome position: 72.72

GC content: 67.19

Gene sequence:

>1143_bases
ATGGTTCTGAAGAATCTGATCAAGGCCAGGGCAGGGCAGCCGGAGCGGCTGTCGGACGATGAGCGGCTGCTCCGCTCGGG
GCTGCTCGCGGGGCTCGAGGCACTGCCCACGGTGGTGCTGGTGCTCGACCGCCGCACGCTGCGCATCGCATTCGCGAATC
CGTCGGCCGAGTCGATGCTCGACATCTCGCGCCGCCAGCTCGCGCAGATGCCGTGGAGCGACCTGTTTCCGAATTCCACC
GAACTGGCCACCACCATCACCGCGATCGGCGAGGGGCGCTTCCAGGCCACCCATCTCGACACCGTGCTCGAGCGGCCCGG
CCGCGAGGCGCTGCACGTGCATGCGATCGTCGGTTTCCTCGAGGACGCGCCCGACTACGTGCTGGTCGAGCTGTTCGAGA
ACGAGCGGCAGTCGCGCACCGACCGCGAGGAGCGGATCCACGACCTGACCGCGGTCAACAAGCACCTGATCCGCAATCTC
GCGCACGAGATCAAGAACCCGCTGGGGGGGATCCGCGGCGCCGCGCAGCTGCTCGAGTTCGAGCTCGGCGAGCGCGAGCG
CGGCGAGCTGCGCGAATACACCCAGGTAATCATCAAGGAATCGGACCGGCTGCAGACGCTGGTCGACCGGCTGCTCGAAC
CGCACCGGCATCCGCATATCGTCGGCGACGTGAACGTCCATGAGGTGTGCGAGCGCGTGCGCGCCGTGATGCTCGCCGAA
TTCCCGCGCGGGCTCACCATCGAGCGCGACTACGACGTCAGCGTGCCGGACCTGCGCGGCGACAAGGAGCAGCTGATCCA
GGCGCTGCTCAACATCGTGCGCAACGCCGCACAGGCCTTGCGCGAGCGGATCGCGCAGGGCGACGCGCGCATCGAGCTGC
GCACGCGCGTGGCGCGCAAGATCACGATCGGCAAGAAACTGTTCAAGCTGGCACTGGACTTGCACGTGATCGACAACGGT
CCCGGCATTCCCGAGGACATCCGCGATCGGATTTTCTACCCGCTCGTGTCGGGGCGCGAGGACGGCAGCGGCCTCGGCCT
CACGCTCGCGCAGACCTTCGTGCAGCAGCACGACGGCACGATCGAGGTGGAAAGCCGTCCGGGCCGCACCGAATTTCAGA
TTTTGCTGCCGCTCGACCATTGA

Upstream 100 bases:

>100_bases
CGCGCGCTGCCGTGAGCCGCGAACATGCGAAGGGGACGGCCTCCGGCCGTCCCTTTTTCGTTGCAGGGTTTTTTCAGCAA
GGAACGGGTTCCCAAGCAAG

Downstream 100 bases:

>100_bases
GCGGCGGCGCCGCGACTCACTGAGACATCTGACCGACCTATGAAGCCGATCTGGATAGTAGACGACGACCAATCGATCCG
CTGGGTGCTTGAAAAGGCGC

Product: Signal transduction histidine kinase, nitrogen specific

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MVLKNLIKARAGQPERLSDDERLLRSGLLAGLEALPTVVLVLDRRTLRIAFANPSAESMLDISRRQLAQMPWSDLFPNST
ELATTITAIGEGRFQATHLDTVLERPGREALHVHAIVGFLEDAPDYVLVELFENERQSRTDREERIHDLTAVNKHLIRNL
AHEIKNPLGGIRGAAQLLEFELGERERGELREYTQVIIKESDRLQTLVDRLLEPHRHPHIVGDVNVHEVCERVRAVMLAE
FPRGLTIERDYDVSVPDLRGDKEQLIQALLNIVRNAAQALRERIAQGDARIELRTRVARKITIGKKLFKLALDLHVIDNG
PGIPEDIRDRIFYPLVSGREDGSGLGLTLAQTFVQQHDGTIEVESRPGRTEFQILLPLDH

Sequences:

>Translated_380_residues
MVLKNLIKARAGQPERLSDDERLLRSGLLAGLEALPTVVLVLDRRTLRIAFANPSAESMLDISRRQLAQMPWSDLFPNST
ELATTITAIGEGRFQATHLDTVLERPGREALHVHAIVGFLEDAPDYVLVELFENERQSRTDREERIHDLTAVNKHLIRNL
AHEIKNPLGGIRGAAQLLEFELGERERGELREYTQVIIKESDRLQTLVDRLLEPHRHPHIVGDVNVHEVCERVRAVMLAE
FPRGLTIERDYDVSVPDLRGDKEQLIQALLNIVRNAAQALRERIAQGDARIELRTRVARKITIGKKLFKLALDLHVIDNG
PGIPEDIRDRIFYPLVSGREDGSGLGLTLAQTFVQQHDGTIEVESRPGRTEFQILLPLDH
>Mature_380_residues
MVLKNLIKARAGQPERLSDDERLLRSGLLAGLEALPTVVLVLDRRTLRIAFANPSAESMLDISRRQLAQMPWSDLFPNST
ELATTITAIGEGRFQATHLDTVLERPGREALHVHAIVGFLEDAPDYVLVELFENERQSRTDREERIHDLTAVNKHLIRNL
AHEIKNPLGGIRGAAQLLEFELGERERGELREYTQVIIKESDRLQTLVDRLLEPHRHPHIVGDVNVHEVCERVRAVMLAE
FPRGLTIERDYDVSVPDLRGDKEQLIQALLNIVRNAAQALRERIAQGDARIELRTRVARKITIGKKLFKLALDLHVIDNG
PGIPEDIRDRIFYPLVSGREDGSGLGLTLAQTFVQQHDGTIEVESRPGRTEFQILLPLDH

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=351, Percent_Identity=41.5954415954416, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI1788549, Length=352, Percent_Identity=27.8409090909091, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1790436, Length=249, Percent_Identity=30.5220883534137, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1790346, Length=243, Percent_Identity=27.5720164609054, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 42983; Mature: 42983

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLKNLIKARAGQPERLSDDERLLRSGLLAGLEALPTVVLVLDRRTLRIAFANPSAESML
CCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHH
DISRRQLAQMPWSDLFPNSTELATTITAIGEGRFQATHLDTVLERPGREALHVHAIVGFL
HHHHHHHHHCCCHHCCCCCHHHHHHHHHHCCCCEEHHHHHHHHHCCCHHHHHHHHHHHHH
EDAPDYVLVELFENERQSRTDREERIHDLTAVNKHLIRNLAHEIKNPLGGIRGAAQLLEF
HCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELGERERGELREYTQVIIKESDRLQTLVDRLLEPHRHPHIVGDVNVHEVCERVRAVMLAE
HCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHH
FPRGLTIERDYDVSVPDLRGDKEQLIQALLNIVRNAAQALRERIAQGDARIELRTRVARK
CCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHH
ITIGKKLFKLALDLHVIDNGPGIPEDIRDRIFYPLVSGREDGSGLGLTLAQTFVQQHDGT
HHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCE
IEVESRPGRTEFQILLPLDH
EEECCCCCCCEEEEEEECCC
>Mature Secondary Structure
MVLKNLIKARAGQPERLSDDERLLRSGLLAGLEALPTVVLVLDRRTLRIAFANPSAESML
CCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHH
DISRRQLAQMPWSDLFPNSTELATTITAIGEGRFQATHLDTVLERPGREALHVHAIVGFL
HHHHHHHHHCCCHHCCCCCHHHHHHHHHHCCCCEEHHHHHHHHHCCCHHHHHHHHHHHHH
EDAPDYVLVELFENERQSRTDREERIHDLTAVNKHLIRNLAHEIKNPLGGIRGAAQLLEF
HCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELGERERGELREYTQVIIKESDRLQTLVDRLLEPHRHPHIVGDVNVHEVCERVRAVMLAE
HCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHH
FPRGLTIERDYDVSVPDLRGDKEQLIQALLNIVRNAAQALRERIAQGDARIELRTRVARK
CCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHH
ITIGKKLFKLALDLHVIDNGPGIPEDIRDRIFYPLVSGREDGSGLGLTLAQTFVQQHDGT
HHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCE
IEVESRPGRTEFQILLPLDH
EEECCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2574025 [H]